References of "Tits, Monique"
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See detailAntioxidant and Antiradical Activities of Manihot esculenta Crantz (Euphorbiaceae) Leaves and Other Selected Tropical Green Vegetables Investigated on Lipoperoxidation and Phorbol-12-myristate-13-acetate (PMA) Activated Monocytes
Tsumbu, César Ndele ULg; Deby-Dupont, Ginette; Tits, Monique ULg et al

in Nutrients (2011), 3(9), 818-838

Abelmoschus esculentus (Malvaceae), Hibiscus acetosella (Malvaceae), Manihot esculenta Crantz (Euphorbiaceae) and Pteridium aquilinum (Dennstaedtiaceae) leaves are currently consumed as vegetables by ... [more ▼]

Abelmoschus esculentus (Malvaceae), Hibiscus acetosella (Malvaceae), Manihot esculenta Crantz (Euphorbiaceae) and Pteridium aquilinum (Dennstaedtiaceae) leaves are currently consumed as vegetables by migrants from sub-Saharan Africa living in Western Europe and by the people in the origin countries, where these plants are also used in the folk medicine. Manihot leaves are also eaten in Latin America and some Asian countries. This work investigated the capacity of aqueous extracts prepared from those vegetables to inhibit the peroxidation of a linoleic acid emulsion. Short chain, volatile C-compounds as markers of advanced lipid peroxidation were measured by gas chromatography by following the ethylene production. The generation of lipid hydroperoxides, was monitored by spectroscopy using N-N′-dimethyl-p-phenylene-diamine (DMPD). The formation of intermediate peroxyl, and other free radicals, at the initiation of the lipid peroxidation was investigated by electron spin resonance, using α-(4-pyridyl-1-oxide)-N-tert-butylnitrone as spin trap agent. The ability of the extracts to decrease the cellular production of reactive oxygen species (ROS) in “inflammation like” conditions was studied by fluorescence technique using 2′,7′-dichlorofluorescine-diacetate as fluorogenic probe, in a cell model of human monocytes (HL-60 cells) activated with phorbol ester. Overall the extracts displayed efficient concentration-dependent inhibitory effects. Their total polyphenol and flavonoid content was determined by classic colorimetric methods. An HPLC-UV/DAD analysis has clearly identified the presence of some polyphenolic compounds, which explains at least partially the inhibitions observed in our models. The role of these plants in the folk medicine by sub-Saharan peoples as well as in the prevention of oxidative stress and ROS related diseases requires further consideration. [less ▲]

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See detail17-O-Acetyl,10-hydroxycorynantheol, a Selective Antiplasmodial Alkaloid Isolated from Strychnos usambarensis Leaves
Cao, Martine ULg; Muganga, Raymond ULg; Tits, Monique ULg et al

in Planta Medica (2011), 77

In the course of our investigations on Strychnos usambarensis leaves in order to isolate isostrychnopentamine, the main alkaloid responsible for the antiplasmodial activity of the plant, a new tertiary ... [more ▼]

In the course of our investigations on Strychnos usambarensis leaves in order to isolate isostrychnopentamine, the main alkaloid responsible for the antiplasmodial activity of the plant, a new tertiary indolic alkaloid has been isolated: 17-O-acetyl, 10-hydroxycorynantheol 1. Its structure was determined by means of spectroscopic and spectrometric methods such as UV, IR, CD, NMR and ESI-MS. 17-O-acetyl, 10-hydroxycorynantheol 1 is one of the most active monomeric indole alkaloid known to date showing an in vitro activity against Plasmodium falciparum close to 5 µM and a high selectivity. [less ▲]

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See detail17-O-acetyl, 10-hydroxycorynantheol, a selective antiplasmodial alkaloid isolated from Strychnos usambarensis leaves
Cao, Martine ULg; Muganga, Raymond ULg; Tits, Monique ULg et al

Conference (2011, May 13)

In the course of our investigations on Strychnos usambarensis leaves in order to isolate isostrychnopentamine, the main alkaloid responsible for the antiplasmodial activity of the plant, a new tertiary ... [more ▼]

In the course of our investigations on Strychnos usambarensis leaves in order to isolate isostrychnopentamine, the main alkaloid responsible for the antiplasmodial activity of the plant, a new tertiary indolic alkaloid has been isolated: 17-O-acetyl, 10-hydroxycorynantheol 1. Its structure was determined by means of spectroscopic and spectrometric methods such as UV, IR, CD, NMR and ESI-MS. 17-O-acetyl, 10-hydroxycorynantheol 1 is one of the most active monomeric indole alkaloid known to date showing an in vitro activity against Plasmodium falciparum close to 5 µM and a high selectivity. [less ▲]

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See detailPharmacognosie volume III
Frederich, Michel ULg; Tits, Monique ULg; Angenot, Luc ULg

Learning material (2011)

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See detailPharmacognosie volume II
Frederich, Michel ULg; Tits, Monique ULg; Angenot, Luc ULg

Learning material (2011)

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See detailQuality Assessment of Polygonum cuspidatum and Polygonum multiflorum by 1H NMR Metabolite Fingerprinting and Profiling Analysis.
Frederich, Michel ULg; Wauters, Jean-Noël ULg; Tits, Monique ULg et al

in Planta Medica (2011), 77

The quality assessment and control of traditional Chinese medicines (TCM) nowadays receives a great deal of attention worldwide and particularly in Europe with its increasing local use. POLYGONUM ... [more ▼]

The quality assessment and control of traditional Chinese medicines (TCM) nowadays receives a great deal of attention worldwide and particularly in Europe with its increasing local use. POLYGONUM CUSPIDATUM Siebold & Zucc. and POLYGONUM MULTIFLORUM Thunb. are two members of the Polygonaceae family, which are widely used as Chinese medicinal plants. The aim of this study was to achieve an overview of the quality of P. CUSPIDATUM and P. MULTIFLORUM samples available on the Chinese market and to identify important metabolites for their discrimination, using (1)H NMR-based metabolomics. (1)H NMR and multivariate analysis techniques were applied to almost 60 plant samples collected in different places in China. Using (1)H NMR metabolomics, it was possible, without previous evaporation or separation steps, to obtain metabolic fingerprints to distinguish between the species. The important metabolites for discrimination were stilbene derivatives. Finally, a clear distinction between the two species was possible and the discriminant metabolites were identified. [less ▲]

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See detailLC-SPE-NMR-MS analysis of Strychnos usambarensis fruits from Rwanda
Cao, Martine ULg; Tits, Monique ULg; Muganga, Raymond et al

in Planta Medica (2010, September), 76(12), 1241-1242

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See detailPharmacognosie volume I
Frederich, Michel ULg; Tits, Monique ULg; Angenot, Luc ULg

Learning material (2010)

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See detailAntioxidant activity of Passiflora edulis and Passiflora alata fruits
Yariwake, J.; Zeraik, M.; Serteyn, Didier ULg et al

in Planta Medica (2010, September), 76(12), 1274-1275

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See detailPlace et importance de la Phytothérapie en thérapeutique moderne
Tits, Monique ULg

Conference (2010, March)

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See detailPharmacognosie - Volume 2
Angenot, Luc ULg; Tits, Monique ULg; Frederich, Michel ULg

Learning material (2010)

Le volume 2 est consacré aux grands classiques de la pharmacognosie dont ont été isolés de très nombreux principes actifs (souvent des alcaloïdes) qui ont marqué l'histoire de la pharmacie et de la ... [more ▼]

Le volume 2 est consacré aux grands classiques de la pharmacognosie dont ont été isolés de très nombreux principes actifs (souvent des alcaloïdes) qui ont marqué l'histoire de la pharmacie et de la pharmacologie.Les chapitres suivants seront abordés: analgésiques (pavot,opium, colchique...); Solanacées à activité parasympatholytiques ( belladone, stramoine, jusquiames...); dérivés de l'ergot de seigle; médicaments des troubles cérébraux de la sénescence ( ginkgo, amaryllidacées à galanthamine...); drogues alcaloïdiques psychoactives ( coca, éphédra, khat, psilocybes, peyotl, harmel, yagé, iboga...); hallucinogènes non alcaloïdiques ( cannabis, sauge des devins..); poisons agissant sur la neurotransmission :cholinergiques ( jaborandi, éséré...), paralysants neuro-musculaires (curares) et antagonistes de la glycine ( noix-vomique); antiparasitaires (malaria et amibiase): quinquinas, armoises, ipécas; anticancéreux : lignanes ( Podophyllum), diterpènes des ifs (Taxus sp), alcaloïdes indoliques ( Catharanthus roseus, Camptotheca ...), divers ( origine marine, épices...); plantes toxiques de l'environnement ( toxicité par contact ou par ingestion) [less ▲]

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See detailPharmacognosie Volume 3
Angenot, Luc ULg; Tits, Monique ULg; Frederich, Michel ULg

Learning material (2010)

Le volume 3 est principalement consacré à la phytothérapie (intérêt et limites) avec des notions concernant aussi bien la médecine traditionnelle que les développements les plus récents ( y compris pour ... [more ▼]

Le volume 3 est principalement consacré à la phytothérapie (intérêt et limites) avec des notions concernant aussi bien la médecine traditionnelle que les développements les plus récents ( y compris pour certains s'appuyant sur des études cliniques) en matière de phytomédicaments. La classification se fait suivant les activités pharmacologiques de ces plantes. Au terme de ce cours, les étudiants doivent pouvoir faire la distinction entre les différentes formes pharmaceutiques à base de produits d'origine naturelle ( poudres, extraits sec et autres), les concentrations en principes actifs et traceurs y étant bien différentes. Les différences entre les médicaments enregistrés et les compléments alimentaires sont également bien mises en évidence car les exigences relatives au contrôle de qualité et à la stabilité des produits finis sont très différentes. A ce sujet l'annexe du volume 3 est consacrée au contrôle de qualité des plantes en insistant sur les méthodes et monographies de la pharmacopée européenne qui joue un rôle essentiel dans ce domaine. [less ▲]

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See detailMetabolomic analysis of Echinacea spp. by (1)H nuclear magnetic resonance spectrometry and multivariate data analysis technique.
Frederich, Michel ULg; Jansen, C.; De Tullio, Pascal ULg et al

in Phytochemical Analysis [=PCA] (2010), 21(1), 61-65

Introduction - The genus Echinacea (Asteraceae) comprises about 10 species originally distributed in North America. Three species are very well known as they are used worldwide as medicinal plants ... [more ▼]

Introduction - The genus Echinacea (Asteraceae) comprises about 10 species originally distributed in North America. Three species are very well known as they are used worldwide as medicinal plants: Echinacea purpurea, E. pallida, E. angustifolia.Objective - To discriminate between these three Echinacea species and E. simulata by (1)H NMR-based metabolomics.Methodology - (1)H NMR and multivariate analysis techniques were applied to diverse Echinacea plants including roots and aerial parts, authentic plants, commercial plants and commercial dry extracts.Results - Using the (1)H NMR metabolomics, it was possible, without previous evaporation or separation steps, to obtain a metabolic fingerprint to distinguish between species.Conclusion - A clear distinction between the three pharmaceutical species was possible and some useful metabolites were identified. Copyright (c) 2009 John Wiley & Sons, Ltd. [less ▲]

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See detailAntiplasmodial and cytotoxic activities of Rwandan medicinal plants used in the treatment of malaria.
Muganga, R.; Angenot, Luc ULg; Tits, Monique ULg et al

in Journal of Ethnopharmacology (2010), 128

AIM OF THE STUDY: In our study, methanol, dichloromethane and aqueous extracts of 13 Rwandan medicinal plants used in the treatment of malaria were tested for in vitro antiplasmodial activity. MATERIALS ... [more ▼]

AIM OF THE STUDY: In our study, methanol, dichloromethane and aqueous extracts of 13 Rwandan medicinal plants used in the treatment of malaria were tested for in vitro antiplasmodial activity. MATERIALS AND METHODS: The growth inhibition of chloroquine-sensitive Plasmodium falciparum strain (3D7) was evaluated using the measurement of lactate dehydrogenase activity. The active extracts were also tested against the chloroquine-resistant Plasmodium falciparum strain (W2) and for cytotoxicity assay using human normal foetal lung fibroblasts (WI-38). RESULTS: The majority of the plants tested showed an antiplasmodial activity and the best results were observed with dichloromethane leaf and flower extracts of Tithonia diversifolia, leaf extract of Microglossa pyrifolia and root extract of Rumex abyssinicus, methanol leaf extract of Fuerstia africana, root bark extracts of Zanthoxylum chalybeum and methanol bark extract of Terminalia mollis. Those extracts were active (IC(50)<15mug/ml) on both chloroquine-sensitive and resistant strains of Plasmodium falciparum. Zanthoxylum chalybeum, Solanecio mannii and Terminalia mollis presented the best selectivity index. CONCLUSIONS: The traditional use of most of the plant evaluated was confirmed by the antiplasmodial test. This study revealed for the first time the antiplasmodial activity of two plants: Terminalia mollis and Rumex abyssinicus. [less ▲]

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See detailEvaluation of 13 selected medicinal plants from Burkina Faso for their antiplasmodial properties.
Jansen, Olivia ULg; Angenot, Luc ULg; Tits, Monique ULg et al

in Journal of Ethnopharmacology (2010), 130

AIM OF THE STUDY: The aim of this study was to evaluate the antiplasmodial properties of 13 plants used against malaria in traditional medicine in Burkina Faso. MATERIALS AND METHODS: In vitro ... [more ▼]

AIM OF THE STUDY: The aim of this study was to evaluate the antiplasmodial properties of 13 plants used against malaria in traditional medicine in Burkina Faso. MATERIALS AND METHODS: In vitro antiplasmodial activity of dichloromethane, methanol and aqueous crude extracts obtained from vegetal samples collected in Burkina Faso was first evaluated on the Plasmodium falciparum 3D7 chloroquine-sensitive strain using a colorimetric method. RESULTS: Thirteen extracts obtained from 8 different species were found to exhibit antiplasmodial activity (IC(50)<50mug/ml). Five species demonstrated a moderate activity (15mug/ml<IC(50)<50mug/ml): Boswellia dalzielii (leaves), Waltheria indica (roots and aerial parts), Bergia suffruticosa (whole plant), Vitellaria paradoxa (bark) and Jatropha gossypiifolia (leaves). The best results were obtained with extracts from the Dicoma tomentosa whole plant, from Psorospermum senegalense leaves and from Gardenia sokotensis leaves. These extracts found to display promising antiplasmodial activity, with IC(50) values ranging from 7.0 to 14.0mug/ml. The most active plant extracts were then tested for in vitro activity on the Plasmodium falciparum W2 chloroquine-resistant strain and also for in vitro cytotoxicity on normal human fibroblasts (WI-38) in order to determine the selectivity index. CONCLUSIONS: Dicoma tomentosa (Asteraceae) and Psorospermum senegalense (Clusiaceae) appeared to be the best candidates for further investigation of their antiplasmodial properties, reported for the first time by this study. [less ▲]

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See detailIn vitro and in vivo antimalarial and cytotoxic activity of five plants used in Congolese traditional medicine.
Lusakibanza, M.; Mesia, G.; Tona, G. et al

in Journal of Ethnopharmacology (2010), 129

AIM OF THE STUDY: The in vitro antiplasmodial activity and cytotoxicity of methanolic and dichloromethane extracts from five Congolese plants were evaluated. The plants were selected following an ... [more ▼]

AIM OF THE STUDY: The in vitro antiplasmodial activity and cytotoxicity of methanolic and dichloromethane extracts from five Congolese plants were evaluated. The plants were selected following an ethnobotanical survey conducted in D.R. Congo and focusing on plants used traditionally to treat malaria. The in vivo antimalarial activity of aqueous and methanolic extracts active in vitro was also determined in mice infected by Plasmodium berghei berghei. MATERIALS AND METHODS: The growth inhibition of Plasmodium falciparum strains was evaluated using the measurement of lactate dehydrogenase activity. The extracts (aqueous, CH(3)OH, EtOH and CH(2)Cl(2)) were prepared by maceration and tested in vitro against the 3D7 (chloroquine sensitive) and W2 (chloroquine resistant) strains of Plasmodium falciparum and against the human normal fetal lung fibroblasts WI-38 to determine the selectivity index. Some extracts were also used at the dose of 300mg/kg to evaluate their activity in mice infected since 4 days by Plasmodium berghei. RESULTS: Two plants presented a very high activity (IC(50)<3mug/ml). These plants were Strychnos icaja roots bark (MeOH and CH(2)Cl(2)) and Physalis angulata leaves (MeOH and CH(2)Cl(2)). One plant (Anisopappus chinensis whole plant, MeOH and CH(2)Cl(2)) presented a high activity (IC50<15mug/ml). The extracts of Anisopappus chinensis and Physalis angulata showed also a good inhibition of parasitemia in vivo. Flavonoids, phenolic acids and terpenes were identified in these plants by a general phytochemical screening method. CONCLUSION: Three plants showed a very interesting antiplasmodial activity (Anisopappus chinensis, Physalis angulata and Strychnos icaja) and one of them showed a good selectivity index (>10, Anisopappus chinensis). Anisopappus chinensis and Physalis angulata were also active in vivo. [less ▲]

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See detailEvaluation des potentialités antiplasmodiales de plantes utilisées en médecine traditionnelle au Burkina Faso
Jansen, Olivia ULg; Nikiéma, Jean-Baptiste; Angenot, Luc ULg et al

Conference (2009, October 07)

Detailed reference viewed: 69 (9 ULg)
See detailPharmacognosie Volume 1
Angenot, Luc ULg; Tits, Monique ULg

Learning material (2009)

Le volume 1 est principalement consacré à la phytochimie. Le cours est subdivisé suivant les différentes classes chimiques des métabolites primaires et secondaires impliqués soit dans l'activité des ... [more ▼]

Le volume 1 est principalement consacré à la phytochimie. Le cours est subdivisé suivant les différentes classes chimiques des métabolites primaires et secondaires impliqués soit dans l'activité des plantes soit dans leur toxicité ou encore présentant des applications en pharmacotechnie ou dans l'alimentation. La connaissance des structures ( basée sur leur origine biosynthétique) est très importante pour évaluer leur solubilité, leur stabilité et ainsi mieux comprendre les processus d'extraction et les méthodes spécifiques de contrôle ( plantes entières ou pulvérisées, , extraits, huiles essentielles, huiles grasses, exsudats...). dans de nombreux cas, une relation structure-activité pharmacologique ou physiologique est établie. [less ▲]

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See detailAntisickling properties of divanilloylquinic acids isolated from Fagara zanthoxyloides Lam. (Rutaceae).
Ouattara, Badiore; Jansen, Olivia ULg; Angenot, Luc ULg et al

in Phytomedicine : International Journal of Phytotherapy and Phytopharmacology (2009), 16(2-3), 125-129

Fagara zanthoxyloides Lam. (syn. Zanthoxylum zanthoxyloides) (Rutaceae) is the most cited Fagara species for the treatment and the prevention of sickle cell disease crisis. Sickle cell anemia (SCA) is a ... [more ▼]

Fagara zanthoxyloides Lam. (syn. Zanthoxylum zanthoxyloides) (Rutaceae) is the most cited Fagara species for the treatment and the prevention of sickle cell disease crisis. Sickle cell anemia (SCA) is a public health problem in many countries particularly in Africa. The present study was designed to evaluate the antisickling properties of three isomeric divanilloylquinic acids (3,4-O-divanilloylquinic acid or burkinabin A; 3,5-O-divanilloylquinic acid or burkinabin B and 4,5-O-divanilloylquinic acid or burkinabin C) identified previously by LC/MS/NMR analysis in the root bark of F. zanthoxyloides [Ouattara et al., 2004. LC/MS/NMR analysis of isomeric divanilloylquinic acids from the root bark of Fagara zanthoxyloides Lam. Phytochemistry 65, 1145-1151]. The three isomers showed interesting antisickling properties which increased from burkinabins A to C. [less ▲]

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