References of "Thonart, Philippe"
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See detailScreening of new microorganisms for pectinases production
Ciza, A.; Sinnaeve, G.; Deconinck, T. et al

Poster (1999, July)

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See detailMicrobiological and chemical study of « Tiéré », senegalese millet fermented food.
Leite, N.; Diop, M.; Tine, E. et al

Poster (1999, July)

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See detailThe monitoring of the enzymatic depolymerisation of pectins by spectroscopic techniques.
Sinnaeve, G.; Ciza, A.; Deconinck, T. et al

Poster (1999, July)

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See detailScreening of pectolytic microorganisms by near infrared reflectance spectroscopy.
Sinnaeve, G.; Ciza, A.; Deconinck, T. et al

Poster (1999, June)

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See detailAntifungal lipopeptides from B. subtilis induce defense-related phenolics in potato
Ongena, Marc ULg; Akpa, E.; Thonart, Philippe ULg et al

Poster (1999, May 04)

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See detailEpidemiological Typing of Extended-Spectrum Beta-Lactamase-Producing Klebsiella Pneumoniae Isolates by Pulsed-Field Gel Electrophoresis and Antibiotic Susceptibility Patterns
Chetoui, H.; Delhalle, E.; Melin, Pierrette ULg et al

in Research in Microbiology (1999), 150(4), 265-72

Over a 16-month period, Klebsiella pneumoniae isolates from 102 patients admitted to a university hospital in Liege (Belgium) produced extended-spectrum beta-lactamases. Pulsed-field gel electrophoresis ... [more ▼]

Over a 16-month period, Klebsiella pneumoniae isolates from 102 patients admitted to a university hospital in Liege (Belgium) produced extended-spectrum beta-lactamases. Pulsed-field gel electrophoresis of genome macrorestriction patterns with XbaI and antibiotic susceptibility patterns subdivided 39 isolates into eight clonally related groups. Two of them were implicated in the course of this outbreak. They were responsible for successive waves of infection or colonization in different wards of the hospital while the others were encountered sporadically. A beta-lactamase with an isoelectric point of 7.6 and consistent with type SHV-2 characterized all nine isolates chosen among both major groups. [less ▲]

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See detailStructural and functional organization of the fengycin synthetase multienzyme system from Bacillus subtilis b213 and A1/3
Steller, S.; Vollenbroich, D.; Leenders, F. et al

in Chemistry & Biology (1999), 6(31-41),

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See detailDevelopment of a technique to determine bile salt hydrolase activity by Lactobacilli spp
Thonart, Philippe ULg; El Mejdoub, T.; Roblain, D. et al

Poster (1999)

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See detailSurface composition and physico-chemical properties of Bacillus subtilis
Ahimou, F.; Boonaert, C.J.P.; Jacques, Philippe ULg et al

Poster (1999)

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See detailStructure, interfacial properties, and functional qualities in foams and emulsions of surfactin, a lipopeptidefrom Bacillus subtilis
Deleu, Magali ULg; Paquot, Michel ULg; Razafindralambo, Hary ULg et al

in Structure, Interfacial properties and functional qualities in foams (1999)

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See detailIn vitro root cultures of Panax ginseng and P-quinquefolium
Kevers, Claire ULg; Jacques, Philippe ULg; Thonart, Philippe ULg et al

in Plant Growth Regulation (1999), 27(3), 173-178

The paper describes a procedure for the initiation, subculture and continued proliferation of adventitious roots of Panax ginseng and Panax quinquefolium, which resemble hairy roots. The technique took ... [more ▼]

The paper describes a procedure for the initiation, subculture and continued proliferation of adventitious roots of Panax ginseng and Panax quinquefolium, which resemble hairy roots. The technique took advantage of the high powerful activity of a new synthetic auxin: benzo[b]selenienyl acetic acid (BSAA). Such initiation from root explants was dependent upon the season, the type and concentration of auxin. The hairy-like roots of ginseng could be subcultured by transfer every 4 weeks to fresh liquid medium either in agitated Erlenmeyer flasks or in bioreactors. Optimal conditions for a continued multiplication (up to 14 per month) were determined. The only practical problem was the limitation of the fresh mass as inoculum: the multiplication rate decreased with the increased quantity of roots. It is postulated that a root growth inhibiting substance was released into the media by the proliferating ginseng hairy roots. [less ▲]

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See detailComputer Simulation Of Surfactin Conformation At A Hydrophobic/Hydrophilic Interface
Gallet, X.; Deleu, Magali ULg; Razafindralambo, Hary ULg et al

in Langmuir (1999), 15(7), 2409-13

Using a molecular modeling method, different conformations of surfactin at a hydrophobic/hydrophilic interface are established. Two conformations of the peptide ring (S1 and S2) provided by NMR ... [more ▼]

Using a molecular modeling method, different conformations of surfactin at a hydrophobic/hydrophilic interface are established. Two conformations of the peptide ring (S1 and S2) provided by NMR experiments built with three different aliphatic chains in folded or extended configurations were studied. For the structures including the S2 peptide ring conformation, the theoretical interfacial molecular area corresponds to the experimental limiting area A0 value obtained with a Langmuir film balance. The peptide ring is positioned in the plane of the interface with the two acidic chains close to each other and protruding in the aqueous phase, and the β-hydroxy fatty acid chain, folded to interact mainly with the Leu2 side chain and also with the Val4 side chain. This design has the largest calculated molecular area and would correspond to the most stable amphipathic structure representing the surfactin experimental behavior in weak compression. [less ▲]

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See detailConversion of green note aldehydes into alcohols by yeast alcohol dehydrogenase
Fauconnier, Marie-Laure ULg; Mpambara, A.; Delcarte, Jérôme et al

in Biotechnology Letters (1999), 21(7), 629-633

‘Green note’ aldehydes were successfully reduced into their corresponding alcohol by commercial yeast alcohol dehydrogenase. Among different yeasts tested for their ability to convert (Z)-3-hexenal into ... [more ▼]

‘Green note’ aldehydes were successfully reduced into their corresponding alcohol by commercial yeast alcohol dehydrogenase. Among different yeasts tested for their ability to convert (Z)-3-hexenal into (Z)-3-hexenol, Pichia anomala gave the best results. Conversion yields higher than 90% were also obtained by directly conducting the reaction in the medium where (Z)-3-hexenal is produced by the action of lipoxygenase and hydroperoxide lyase on <br />linolenic acid. [less ▲]

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See detailThe structure of two fengycins from Bacillus subtilis S499.
Schneider, J.; Taraz, K.; Budzikiewicz, H. et al

in Zeitschrift für Naturforschung. C, Journal of Biosciences (1999), 54(11), 859-66

The structures of the two fengycins, lipopeptides from Bacillus subtilis, were elucidated by spectroscopic methods and chemical degradation. They show a close structural relationship to the plipastatins ... [more ▼]

The structures of the two fengycins, lipopeptides from Bacillus subtilis, were elucidated by spectroscopic methods and chemical degradation. They show a close structural relationship to the plipastatins from Bacillus cereus differing only in the stereochemistry of the Tyr residues. [less ▲]

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See detailNanometer scale organization of mixed surfactin/phosphatidylcholine monolayers
Deleu, Magali ULg; Paquot, Michel ULg; Jacques, Philippe ULg et al

in Biophysical Journal (1999), 77(4), 2304-2310

Mixed monolayers of the surface-active lipopeptide surfactin-C-15 and of dipalmitoyl phosphatidylcholine (DPPC) were deposited on mica and their nanometer scale organization was investigated using atomic ... [more ▼]

Mixed monolayers of the surface-active lipopeptide surfactin-C-15 and of dipalmitoyl phosphatidylcholine (DPPC) were deposited on mica and their nanometer scale organization was investigated using atomic force microscopy (AFM) and x-ray photoelectron spectroscopy (XPS). AFM topographic images revealed phase separation for mixed monolayers prepared at 0.1, 0.25, and 0.5 surfactin molar ratios. This was in agreement with the monolayer properties at the air-water interface indicating a tendency of the two compounds to form bidimensional domains in the mixed systems. The step height measured between the surfactin and the DPPC domains was 1.2 +/- 0.1 nm, pointing to a difference in molecular orientation: while DPPC had a vertical orientation, the large peptide ring of surfactin was lying on the mica surface. The N/C atom concentration ratios obtained by XPS for pure monolayers were compatible with two distinct geometric models: a random layer for surfactin and for DPPC, a layer of vertically-oriented molecules in which the polar headgroups are in contact with mica. XPS data for mixed systems were accounted for by a combination of the two pure monolayers, considering respective surface coverages that were in excellent agreement with those measured by AFM. These results illustrate the complementarity of AFM and XPS to directly probe the molecular organization of multicomponent monolayers. [less ▲]

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See detailInterfacial and emulsifying properties of lipopeptides from Bacillus subtilis
Deleu, Magali ULg; Razafindralambo, Hary ULg; Popineau, Y. et al

in Colloids and Surfaces A : Physicochemical and Engineering Aspects (1999), 152(1-2), 3-10

The fundamental surface-active properties at the oil/water interface and emulsifying properties of surfactin, iturin A and fengycin, lipopeptides from Bacillus subtilis, were investigated. All ... [more ▼]

The fundamental surface-active properties at the oil/water interface and emulsifying properties of surfactin, iturin A and fengycin, lipopeptides from Bacillus subtilis, were investigated. All lipopeptides reduce rapidly the dynamic interfacial tension. Among lipopeptide families, surfactin is the most effective in terms of fundamental dynamic and equilibrium interfacial properties. Lipopeptides present intermediate properties in comparison with sodium dodecyl sulfate and beta-lactoglobulin concerning the stabilizing effect towards creaming-flocculation and the resistance to coalescence. Among lipopeptides, iturin A seems to show the best resistance to creaming-flocculation whereas fengycin exhibits the highest resistance to coalescence properties. (C) 1999 Elsevier Science B.V. All rights reserved. [less ▲]

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See detailImproving The Catabolic Functions Of Desiccation-Tolerant Soil Bacteria
Weekers, F.; Jacques, Philippe ULg; Springael, D. et al

in Applied Biochemistry and Biotechnology (1999), 77-9

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