References of "Thonart, Philippe"
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See detailMicroorganisms from aphids attract hoverflies and enhance their efficacy
Leroy, Pascal ULg; Sabri, Ahmed ULg; Heuskin, Stéphanie ULg et al

Poster (2011, March 05)

Aphids are some of the most serious pests of crops worldwide, causing major yield and economic losses. They damage plants by feeding on the phloem sap, excreting copious amounts of honeydew and, in some ... [more ▼]

Aphids are some of the most serious pests of crops worldwide, causing major yield and economic losses. They damage plants by feeding on the phloem sap, excreting copious amounts of honeydew and, in some cases, vectoring plant diseases. Here, we report the first isolation of a bacterium from the pea aphid Acyrthosiphon pisum honeydew, Staphylococcus sciuri, which is involved in the release of semiochemicals acting as a kairomone for aphid natural enemies. These semiochemicals were identified by Solid-Phase Microextraction (SPME) and Gas Chromatography-Mass Spectrometry (GC-MS). Our results show that this bacterium plays a key role in the interactions between aphids and natural enemies because it is the direct source of volatiles used by the aphidophagous hoverfly Episyrphus balteatus (De Geer) (Diptera: Syrphidae) to locate its aphid prey. Through wind-tunnel experiments, some specific semiochemicals produced by S. sciuri were identified as significant attractants and ovipositional stimulants. Also, assays under greenhouses and in potato fields have demonstrated that a culture medium containing the bacterium S. sciuri strongly attracts and induces the oviposition of hoverflies, enhancing their efficiency as biological control agents. The use of this no pathogenic bacterium could provide a very novel approach towards enhancing the efficacy of biological control agents to control aphids in field crops and greenhouse systems. [less ▲]

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See detailIn vitro culture of the parasitic wasp Aphidius ervi: Sweet dream or Reality
Sabri, Ahmed ULg; Leroy, Pascal ULg; Haubruge, Eric ULg et al

Poster (2011, March 03)

Aphidius ervi is an entomophagous parasitoid and represents an effective parasitoid of several aphid species that cause great damages in agriculture. Several investigations, for its in vitro mass ... [more ▼]

Aphidius ervi is an entomophagous parasitoid and represents an effective parasitoid of several aphid species that cause great damages in agriculture. Several investigations, for its in vitro mass production, have achieved a limited success and suggest that in vitro culture of this valuable biological control agent is rather closer to a dream than reality. Our work provides a chronological study of A. ervi development, from the oviposition until hatching of the first instar larva, in the body of the pea aphid Acyrthosiphon pisum. We show that this parasitoid has some characteristics in its embryological development that are rather complex and different from most other reported insects, which can be phylogenetically very close. Some of these characteristics concern extraembryonic membranes and could be among the causes of the limited success achieved in the in vitro culture. [less ▲]

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See detailBiomass production and small-scale testing of freeze-dried lactic acid bacteria starter strains for cassava fermentations
Edward, V.; Huch, M.; Dortu, C. et al

in Food Control (2011), 22(3-4), 389395

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See detailAphid-host plant interactions: Does aphid honeydew exactly reflect the host plant amino acid composition?
Leroy, Pascal ULg; Wathelet, Bernard ULg; Sabri, Ahmed ULg et al

in Arthropod-Plant Interactions (2011), 5

Plants provide aphids with unbalanced and low concentrations of amino acids. Likely, intracellular symbionts improve the aphid nutrition by participating to the synthesis of essential amino acids. To ... [more ▼]

Plants provide aphids with unbalanced and low concentrations of amino acids. Likely, intracellular symbionts improve the aphid nutrition by participating to the synthesis of essential amino acids. To compare the aphid amino acid uptakes from the host plant and the aphids amino acid excretion into the honeydew, host plant exudates (phloem + xylem) from infested and uninfested Vicia faba L. plants were compared to the honeydew produced by two aphid species (Acyrthosiphon pisum Harris and Megoura viciae Buckton) feeding on V. faba. Our results show that an aphid infestation modifies the amino acid composition of the infested broad bean plant since the global concentration of amino acids significantly increased into the host plant in response to aphid infestations. Specifically, the concentrations of two amino acids glutamine and asparagine were strongly enhanced. The amino acid profiles from honeydews were similar for the two aphid species, but the concentrations found into the honeydews were generally lower than those measured in the exudates of infested plants (aphids uptakes). This work also highlights that aphids take large amounts of amino acids into the host plant, especially glutamine and asparagine which are converted into glutamic and aspartic acids but also into other essential amino acids. The amino acid profiles differed between the host plant exudates and the aphid excretion product. Finally, this study highlights that the pea aphid - a “specialist” for the V. faba host plant - induced more important modifications into the host plant amino acid composition than the “generalist” aphid M. viciae. [less ▲]

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See detailIn vitro antagonistic activity evaluation of Lactic Acid Bacteria (LAB) combined with cellulase enzyme against Campylobacter jejuni growth in co-culture
Dubois Dauphin, Robin ULg; vandeplas, Sabrina; Didderen, Isabelle et al

in Journal of Microbiology and Biotechnology (2011), 21(1), 62-70

The antibacterial effects of nine Lactic Acid Bacteria (LAB) on Campylobacter jejuni were investigated using agar gel diffusion and co-culture assay. Inhibition potential was not the same between both ... [more ▼]

The antibacterial effects of nine Lactic Acid Bacteria (LAB) on Campylobacter jejuni were investigated using agar gel diffusion and co-culture assay. Inhibition potential was not the same between both techniques. Only two LAB, Lb. pentosus CWBI B78 and E. faecium THT, showed an anti-campylobacter activity in co-culture assay in using dehydrated poultry excreta mixed with ground straw (DPE/GS) as the only growth substrate source. It was observed that the complementation with Cellulase A complex (Beldem S.A.) of this medium enhanced antimicrobial effect of both bacteria. The co-culture medium acidification was correlated with the concentration in supplemented enzyme. The antibacterial effect was characterized by the production of lactic acid by the homofermentative E. faecium THT and the lactic and acetic acids production by the heterofermentative Lb. pentosus CWBI B78. The antagonistic properties from bacteria-enzyme cooperation could reduce the prevalence of Campylobacter consequently the risk of human infection. [less ▲]

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See detailAction des cultures protectrices : cas des germes lactiques sur la flore alimentaire indésirable.
Privat, Kouakou; Thonart, Philippe ULg

in Biotechnologie, Agronomie, Société et Environnement = Biotechnology, Agronomy, Society and Environment [=BASE] (2011), 15(2), 339-348

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See detailBiochemistry of lactone formation in yeast and fungi and its utilisation for the production of flavour and fragrance compounds
Romero-Guido, C.; Belo, I.; Ta, T. M. N. et al

in Applied Microbiology & Biotechnology (2011), 89

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See detailProduction potentielle de bioéthanol, de biométhane et de pellets à partir des déchets de biomasse lignocellulosique du bananier (Musa spp.) au Cameroun
Kamdem, Irenée ULg; Tomekpe, Kodjo; Thonart, Philippe ULg

in Biotechnologie, Agronomie, Société et Environnement = Biotechnology, Agronomy, Society and Environment [=BASE] (2011), 15(3), 471-483

Like most African countries who are producers and exporters of banana, Cameroon is facing a major energy deficit. Yet, the country is generating annually about 4,500,000 tons of fresh banana plant ... [more ▼]

Like most African countries who are producers and exporters of banana, Cameroon is facing a major energy deficit. Yet, the country is generating annually about 4,500,000 tons of fresh banana plant lignocellulosic waste biomass matter equivalent to 402,750 tons of dry matter. The dry matter contained about 80,57% organic matter which are not exploited. Under the sustainable development, which is linked to environmental protection, the biotransformation of these residues can potentially produce about 93,800; 92,133; 447,500 tons of bioethanol, biomethane and pellets respectively. The waste transformation could reduce the energy deficit and create jobs opportunities. Productions of this renewable energy or biofuel also constitute a new area which could assure an important source of income for the banana cultivators and the entire country. [less ▲]

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See detailDimensionnement et extrapolation des bioréacteurs sur base de paramètres physiologiques : cas de la production de lipase par Yarrowia lipolytica
Kar, Tambi ULg; Delvigne, Frank ULg; Destain, Jacqueline ULg et al

in Biotechnologie, Agronomie, Société et Environnement = Biotechnology, Agronomy, Society and Environment [=BASE] (2011), 15(4), 585-595

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See detailEarthworms smell microorganisms in soil
Zirbes, Lara ULg; Verheggen, François ULg; Mescher, Mark et al

Poster (2011)

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See detailSynthesis by sol-gel process of visible light sensitive-TiO2 for the degradation of pollutants and microorganisms
Tasseroul, Ludivine ULg; Lambert, Stéphanie ULg; Páez Martínez, Carlos ULg et al

Poster (2011)

Since the discovery of photocatalytic decomposition of water on TiO2 electrodes by Fujishima and Honda [1], heterogenous photocatalysis has been widely studied for environmental applications ... [more ▼]

Since the discovery of photocatalytic decomposition of water on TiO2 electrodes by Fujishima and Honda [1], heterogenous photocatalysis has been widely studied for environmental applications. Photocatalytic processes are new technologies for waste water treatment because new compounds (alkanes, pesticides, dyes, etc.) in effluents are not readily degraded by the conventional treatments [2]. Oxidative processes can completely destroy organic pollutants [3] or microorganisms (bacteria, viruses, fungi, etc.) [4,5]. At this moment, the commercial Degussa P25 is the mostly used photocatalyst. Nevertheless, it requires UV light to be activated, that is why scientists try to activate TiO2 under visible light. The aim of this study is to synthesize photocatalysts activated by visible light and to evaluate their activity for the degradation of pollutants and microorganisms. The sol-gel process allows the direct introduction of visible light sensitive species like porphyrins inside the TiO2 matrix during the synthesis [6]. Two tetracarboxyphenyl porphyrins are synthesized for the sensitization of TiO2 to visible light : the metal free tetracarboxyphenyl porphyrin and the nickel tetracarboxyphenyl porphyrin. These porphyrins are characterized by 1H NMR, FT-IR and UV/Vis spectroscopies. They are introduced during the sol-gel synthesis of TiO2 matrix. Diffuse reflectance and FT-IR spectroscopies are used to ensure that porphyrins are incorporated inside the matrix of TiO2. Indeed, characteristic porphyrins peaks appear on spectra. The cristallinity and specific surfaces of catalysts are also determined by XRD and nitrogen adsorption-desorption measurements respectively. The depolluting efficiency of photocatalysts is evaluated for the degradation in water of p-nitrophenol, which is listed as one of the 114 organic pollutants by the Environmental Protection Agency in the US. After 6 h under visible light, 40 % of p-nitrophenol are degraded with some catalysts. The activity is influenced by i) the presence of porphyrins : the photoactivity increases with the amount of porphyrins ; ii) the nature of porphyrins : nickel porphyrins are more active than free metal porphyrins ; and iii) the crystallinity of catalysts : amorphous catalysts are less active than crystallized samples. The photocatalysts with the highest degradation rating of dyes will be selected for the degradation in water of Escherichia coli and Lactobacillus acidophilus. [1] A. Fujishima, K. Honda, Nature 238, 37-38 (1972) [2] P. Gogate, A. Pandit, Advances in Environmental Research 8, 501-551 (2004). [3] D. Chen, A. Ray, Water Research 32, 3223-3234 (1998) [4] R. Watts, S. Kong, M. Orr, G. Miller, B. Henry, Water Research 29, 95-100 (1995). [5] J. Hong, M. Otaki, Journal of Bioscience and Bioengineering 101, 185-189 (2006). [6] C. Wang, J. Li, G. Mele, G.M. Yang, F.-X. Zhang, L. Palmisano, G. Vasapollo, Applied catalysis 78, 218-226 (2007). [less ▲]

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See detailBeneficial effect of the rhizosphere microbial community for plant growth and health.
Nihorimbere, V.; Ongena, Marc ULg; Smargiassi, M. et al

in Biotechnologie, Agronomie, Société et Environnement = Biotechnology, Agronomy, Society and Environment [=BASE] (2011), 15(2), 327-337

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See detailApplicability of GFP Microbial Whole Cell Biosensors to Bioreactor Operations : Mathematical Modeling and Related Experimental Tools
Delvigne, Frank ULg; Brognaux, Alison ULg; Gorret, Nathalie et al

in Biosensors : emerging materials and applications (2011)

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See detailHigh-level production of extracellular lipase by Yarrowia lipolytica mutants from methyl oleate
Darvishi, F.; Destain, Jacqueline ULg; Nahvi, I. et al

in New Biotechnology (2011), 28(6), 756-760

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See detailImprovement of the Composition of Tunisian Myrtle Berries (Myrtus 2 communis L.) Alcohol Extracts
Snoussi, A.; Ben haj Koubaier, H.; Essaidi, Ismahen et al

in Journal of Agricultural and Food Chemistry (2011), 3b2(9),

Detailed reference viewed: 17 (5 ULg)