References of "Thiry, Marc"
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See detailHuman papillomavirus entry into NK cells requires CD16 expression and triggers cytotoxic activity and cytokine secretion.
Renoux, Virginie ULg; Bisig, Bettina ULg; Langers, Inge ULg et al

in European journal of immunology (2011), 41(11), 3240-3252

Human papillomavirus (HPV) infections account for more than 50% of infection-linked cancers in women worldwide. The immune system controls, at least partially, viral infection and around 90% of HPV ... [more ▼]

Human papillomavirus (HPV) infections account for more than 50% of infection-linked cancers in women worldwide. The immune system controls, at least partially, viral infection and around 90% of HPV-infected women clear the virus within two years. However, it remains unclear which immune cells are implicated in this process and no study has evaluated the direct interaction between HPVs and NK cells, a key player in host resistance to viruses and tumors. We demonstrated an NK cell infiltration in HPV-associated pre-neoplastic cervical lesions. Since HPVs cannot grow in vitro, virus-like particles (VLPs) were used as a model for studying the NK cell response against the virus. Interestingly, NK cells displayed higher cytotoxic activity and cytokine production (TNF-alpha and IFN-gamma) in the presence of HPV-VLPs. Using flow cytometry and microscopy we observed that NK cell stimulation was linked to rapid VLP entry into these cells by macropinocytosis. Using CD16(+) and CD16(-) NK cell lines and a CD16-blocking antibody, we demonstrated that CD16 is necessary for HPV-VLP internalization, as well as for degranulation and cytokine production. Thus, we show for the first time that NK cells interact with HPVs and can participate in the immune response against HPV-induced lesions. [less ▲]

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See detailDigging deeper into lymphatic vessel formation in vitro and in vivo
Detry, Benoît ULg; Bruyère, F.; Erpicum, Charlotte ULg et al

in BMC Cell Biology (2011), 12

Background Abnormal lymphatic vessel formation (lymphangiogenesis) is associated with different pathologies such as cancer, lymphedema, psoriasis and graft rejection. Lymphatic vasculature displays ... [more ▼]

Background Abnormal lymphatic vessel formation (lymphangiogenesis) is associated with different pathologies such as cancer, lymphedema, psoriasis and graft rejection. Lymphatic vasculature displays distinctive features than blood vasculature, and mechanisms underlying the formation of new lymphatic vessels during physiological and pathological processes are still poorly documented. Most studies on lymphatic vessel formation are focused on organism development rather than lymphangiogenic events occurring in adults. We have here studied lymphatic vessel formation in two in vivo models of pathological lymphangiogenesis (corneal assay and lymphangioma). These data have been confronted to those generated in the recently set up in vitro model of lymphatic ring assay. Ultrastructural analyses through Transmission Electron Microscopy (TEM) were performed to investigate tube morphogenesis, an important differentiating process observed during endothelial cell organization into capillary structures. [less ▲]

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See detailSpatio-temporal localization of the cytoskeleton during auditory organ development in mammalia
Johnen, Nicolas ULg; Thelen, Nicolas ULg; Cloes, Marie ULg et al

Poster (2011, March 31)

The auditory organ, the organ of Corti (OC), is a highly specialized structure composed by specific cellular types. The sensory cells (HC) are characterized by stereocilia at their apex and are necessary ... [more ▼]

The auditory organ, the organ of Corti (OC), is a highly specialized structure composed by specific cellular types. The sensory cells (HC) are characterized by stereocilia at their apex and are necessary for the sound perception. Theses cells are supported by supporting cells. Based on their morphology and physiology, at least four types of supporting cells (SC) can be identified in the OC: inner and outer pillar cells (PC), phalangeal cell and Deiter’s cells. Sensory and supporting cells possess characteristic cytoskeleton proteins in direct relation with their morphological features and their development. Indeed, this organ had morphological changes such as the setting up of the sensory epithelium after the birth or the openings of the Corti’s tunnel at P8 and of the Nuel’s spaces at P10. In the present study, by using confocal microscopy, we investigated the spatio-temporal localization of the three cellular cytoskeletal filaments : microtubules (β-1, 2, 3, 4-tubulin), microfilaments (cytoplasmic β- and γ-actin) and intermediate filaments (CK4, 5, 7, 8, CKpan and vimentin) during the development of the OC in rat from the embryonic day 18 (E18) to the post-natal day 25 (P25). The immunolabellings indicated clearly that β-1, 2, 3-tubulins were only present the SC and nervous fibers during development whereas β-4-tubulin was found firstly in the HC and then in the SC. The two actin-isotypes were detected in the HC apex but were also seen in the PC from P8 to P25 for β-actin isoform and in the basal membrane from E18 to P8 for the γ-actin isoform. All intermediate filament proteins were only found in the SC, especially between P8 and P12. Our results show that the localization of the cytoskeleton proteins during the auditory organ development depends on the cellular type and the developmental stage. A profound modification of cytoskeleton occurs between P8 and P12. [less ▲]

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See detailNucleolar structure across evolution : the transition between bi- and tricompartmentalized nucleoli lies within the class Reptilia
Franck, Claire; Lamaye, Françoise; Thelen, Nicolas ULg et al

Poster (2011, January 31)

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See detailSpatio-temporal localization of the glial fibrillary acidic protein (GFAP) in the spiral ganglion from the 16th embryonic day until the 25th postnatal day in rats
Cloes, Marie ULg; Thelen, Nicolas ULg; Johnen, Nicolas ULg et al

Poster (2011, January 31)

The spiral ganglion (SG) is responsible for the conduction of the information between the sensory epithelium of the auditory organ (the organ of Corti) and the central nervous system. The origin and ... [more ▼]

The spiral ganglion (SG) is responsible for the conduction of the information between the sensory epithelium of the auditory organ (the organ of Corti) and the central nervous system. The origin and nature of the SG glial cells in mammals are barely known, although glial cells are essential to the development and the working of the nervous system. Using confocal microscopy, we studied the spatio-temporal distribution of the GFAP in the rat SG from the 16th embryonic day (E16) until the 25th postnatal day (P25). We performed a double-labelling experiment targeting GFAP- and betaIIItubulin-positive cells. BetaIII-tubulin is used for the labelling of (pro)neural cells, according to a previous preliminary study from our team. Our first results show clearly that the GFAP is expressed in the SG from P0 until P25. A homogenous labelling is found in the cytoplasm of a few dispersed unidentified cells among the (pro)neurons, whereas a granular labelling appears among a group of cells neighbouring the bundle of fibers innervating the organ of Corti. The identity of the GFAP-positive cells will be further investigated by electron microscopy. The reason why the labelling of the GFAP adopts those two different aspects is still unknown. Moreover, it seems that, surprisingly, some cells of the ganglion are not labelled by either marker. The possibility that such cells correspond to fibroblasts will be tested thanks to the labelling of vimentin. [less ▲]

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See detailDistribution of glycogen during the development of the organ of Corti
Thelen, Nicolas ULg; Cloes, Marie ULg; Johnen, Nicolas ULg et al

Poster (2011, January 31)

Although the structure of the auditory organ in mature mammals, the organ of Corti, is clearly established, its development is far from being elucidated. Using cytochemical methods at the light and ... [more ▼]

Although the structure of the auditory organ in mature mammals, the organ of Corti, is clearly established, its development is far from being elucidated. Using cytochemical methods at the light and electron microscope levels, we examined the spatiotemporal distribution of polysaccharides during the development of the organ of Corti in rats from embryonic day 16 (E16) to postnatal day 15 (P15). At E16, small polysaccharide inclusions were detected in the cytoplasm of the future inner pillar cells by electron microscope only. These inclusions became obvious at the light microscope level at E17. At E19, the polysaccharide deposits were important within the inner pillar cells and they arose in the Hensen cells cytoplasm. Polysaccharide accumulations also appeared in the outer pillar cells and the Deiters cells from P3-P4. As the organ of Corti developed, the amount of polysaccharide inclusions within the inner and outer pillar cells decreased. At P15, large amount of polysaccharide deposits were visible in the Deiters cells whereas they had almost disappeared from the inner and outer pillar cells. Finally, we showed that the polysaccharide deposits present in the developing organ of Corti are PAS-positive and can be digested with a salivary amylase, suggesting that they are essentially constituted of glycogen. [less ▲]

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See detailSpatio-temporal localisation of β-actin and γ-actin isoforms during the development of the organ of Corti in rat from the embryonic day 18 (E18) to the post-natal day 25 (P25).
Johnen, Nicolas ULg; Thelen, Nicolas ULg; Cloes, Marie ULg et al

Poster (2011, January 31)

The auditory organ, the organ of Corti (OC), is a highly specialized structure composed by specific cellular types. The sensory cells (HC) are characterized by stereocilia at their apex and are necessary ... [more ▼]

The auditory organ, the organ of Corti (OC), is a highly specialized structure composed by specific cellular types. The sensory cells (HC) are characterized by stereocilia at their apex and are necessary for the sound perception. Theses cells are supported by supporting cells. Based on their morphology and physiology, at least four types of supporting cells can be identified in the OC: inner and outer pillar cells (PC), phalangeal cell and Deiter’s cells. Sensory and supporting cells have cytoskeletons containing β-actin and γ-actin isoforms. In the adult mammalian cochlea, amounts of γ-actin increase and β-actin decrease in the order: outer pillar cells, inner pillar cells, Deiters’ cells and hair cells. In sensory cells, γ-actin appears to be the most prominent component with an apparent γ-actin/β-actin ratio of approximately 2:1 (Hofer et al., 1997). β-actin is present in the cuticular plate but is more concentrated in the stereocilia, especially at the base where the stereocilia insert into the cuticular plate. The amount of γ-actin differs less between these structures, the stereocilia and cuticular plate, although its expression is apparently higher towards the tip of stereocilia and it is the predominant isoform of the hair cell's lateral wall (Furness et al., 2005). The differential subcellular localization of two actin isoforms suggests they may play different functions in auditory organ. In the brain, β-actin is restricted to dynamic structures whereas γ-actin is more ubiquitously distributed and occurs in relatively quiescent regions (Micheva et al., 1998). In the present study, by using confocal microscopy, we investigated the spatio-temporal localisation of β-actin and γ-actin isoforms during the development of the OC in rat from the embryonic day 18 (E18) to the post-natal day 25 (P25). Our results indicated that the labelling for both actin isoforms changed during the OC development. Between E18 and P25, we observed a labelling for β-actin in the apical region of the HC. Between P8 and P25, the feet of PC are also β-actin-positive. Unlike β-actin, between E18 and P10, γ-actin is detected in the basal region of supporting cells. Between P12 and P25, the labelling for γ-actin is preferentially localized in the apical surface of the HC. Our results revealed that during development β-actin isoform preceded γ-actin isoform in the apical region of HC. They also suggest that γ-actin isoform might be involved in attachment of supporting cells with their basal membrane and that β-actin isoform might play a role in PC reorganization during the formation of Corti tunnel. [less ▲]

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See detailLa structure géométrique de l’ADN
Thiry, Marc ULg; Cloes, Marie

in Tangente (2011), 42

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See detailLes spirales cachées du vivant
Cloes, Marie ULg; Thiry, Marc ULg

in Tangente (2011), 42

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See detailLa structure géométrique de l’ADN
Thiry, Marc ULg; Cloes, Marie ULg

in Tangente (2011), 42

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See detailMacropinocytosis of human papillomaviruses in natural killer cells via CD16 induces cytotoxic granule exocytosis and cytokine secretion
Renoux, V; Langers, I; Clémenceau, B et al

Poster (2011)

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See detailTémoignage 2: les mathématiques dans le programme du cursus en sciences biologiques
Thiry, Marc ULg

Diverse speeche and writing (2011)

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See detailThe nucleolus: When 2 became 3.
Thiry, Marc ULg; Lamaye, Françoise ULg; Lafontaine, Denis L. J.

in Nucleus (2011), 2(4),

Though the nucleolus is considered today as a multifunctional domain, its primary function is ribosome biogenesis. We have shown at the ultrastructural level that there are primarily two types of ... [more ▼]

Though the nucleolus is considered today as a multifunctional domain, its primary function is ribosome biogenesis. We have shown at the ultrastructural level that there are primarily two types of nucleolar organization: nucleoli containing three components in amniotes and two components in all other eukaryotes. In a recent report we made the additional, and surprising, finding that both types of nucleolar arrangement are found among living reptiles, viz. a bicompartmentalized nucleolus in turtles and a tricompartmentalized nucleolus in lizards, crocodiles and snakes. This latter organization occurs regardless of the species, the tissue or the developmental stages analyzed. These results are compatible with the view that the transition between bipartite and tripartite nucleoli coincided with the emergence of the amniotes within the Reptilia. They also support the previous hypothesis that turtles are primitive reptiles. The emergence in amniote vertebrates of a third nucleolar compartment might have imparted novel regulatory functions to the nucleolus, as well as perhaps, expanding the adaptability of ribosome synthesis to an ever changing environment, thus, enhancing the overall fitness of amniotic vertebrates. [less ▲]

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See detailNucleolar structure across evolution: the transition between bi- and tri-compartmentalized nucleoli lies within the class Reptilia.
Lamaye, Francoise; Galliot, Sonia; Alibardi, Lorenzo et al

in Journal of Structural Biology (2011), 174(2), 352-9

Two types of nucleolus can be distinguished among eukaryotic cells: a tri-compartmentalized nucleolus in amniotes and a bi-compartmentalized nucleolus in all the others. However, though the nucleolus ... [more ▼]

Two types of nucleolus can be distinguished among eukaryotic cells: a tri-compartmentalized nucleolus in amniotes and a bi-compartmentalized nucleolus in all the others. However, though the nucleolus' ultrastructure is well characterized in mammals and birds, it has been so far much less studied in reptiles. In this work, we examined the ultrastructural organization of the nucleolus in various tissues from different reptilian species (three turtles, three lizards, two crocodiles, and three snakes). Using cytochemical and immunocytological methods, we showed that in reptiles both types of nucleolus are present: a bi-compartmentalized nucleolus in turtles and a tri-compartmentalized nucleolus in the other species examined in this study. Furthermore, in a given species, the same type of nucleolus is present in all the tissues, however, the importance and the repartition of those nucleolar components could vary from one tissue to another. We also reveal that, contrary to the mammalian nucleolus, the reptilian fibrillar centers contain small clumps of condensed chromatin and that their surrounding dense fibrillar component is thicker. Finally, we also report that Cajal bodies are detected in reptiles. Altogether, we believe that these results have profound evolutionarily implications since they indicate that the point of transition between bipartite and tripartite nucleoli lies at the emergence of the amniotes within the class Reptilia. [less ▲]

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See detailHPV triggers NK cell cytotoxic activity and cytokine secretion
Jacobs, Nathalie ULg; Renoux, Virginie ULg; Bisig, Bettina ULg et al

Conference (2011)

Background The immune system controls, at least partially, human papillomavirus (HPV) infection and subsequent tumor development as demonstrated by a higher tumor prevalence in immunodeficient patients ... [more ▼]

Background The immune system controls, at least partially, human papillomavirus (HPV) infection and subsequent tumor development as demonstrated by a higher tumor prevalence in immunodeficient patients. Around 90% of HPV-infected women will clear the virus within two years. However, it remains unclear which immune cells are implicated in this process and no study has been performed evaluating the direct interaction between HPV and Natural Killer (NK) cells although these cells play a key role in host resistance to virus and tumor. Methods/Results By immunochemistry, we demonstrated an NK cell infiltration in HPV+ squamous pre-neoplasic lesions. Since HPV cannot grow in vitro, virus-like particles (VLP) were used as a model for studying the NK cell response against the virus. Interestingly, NK cells displayed a higher cytotoxic activity (CD107 and chromium release assays) and cytokine production (TNF-α and IFN-γ) in the presence of HPV-VLP. Uptake of HPV-VLP by dendritic cells (DC) has been shown to induce their activation, therefore, we investigated by flow cytometry and microscopy whether the stimulation of NK cell activity is linked to VLP internalization. We observed a faster entry into these cells compared to DC. Furthermore, virus uptake by NK cells is mediated by macropinocytosis, whereas this entry is dependent on clathrin or caveolin endocytosis pathways in DC. Using NK cell lines expressing or not CD16 and blocking antibody, we demonstrated that CD16 is necessary for HPV-VLP internalization, but also for degranulation and cytokine production. Conclusion Thus, we show for the first time that NK cells interact with HPV and could participate in the immune response against HPV-induced tumors. [less ▲]

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See detailSPATIO-TEMPORAL LOCALIZATION OF BETA TUBULIN III IN THE ORGAN OF CORTI AND IN THE SPIRAL GANGLIA BETWEEN THE EMBRYONIC DAY (E18) AND THE POST-NATAL DAY (P25) IN RAT.
Johnen, Nicolas ULg; Thelen, Nicolas ULg; Cloes, Marie ULg et al

Poster (2010, October 22)

The mammalian auditory organ, the organ of Corti (OC), is composed of mechanosensory hair cells and nonsensory supporting cell types. Based on their morphology and physiology, at least two types of ... [more ▼]

The mammalian auditory organ, the organ of Corti (OC), is composed of mechanosensory hair cells and nonsensory supporting cell types. Based on their morphology and physiology, at least two types of sensory cells can be identified in the OC: inner and outer hair cells. The structure of this organ is well reported in adult but its development is still little-known. By using confocal microscopy, we studied the spatial-temporal distribution of beta tubulin III during the differentiation of the OC in rat from the embryonic day 18 (E18) to the postnatal day (P25). The beta tubulin III is typical for neural cells in the OC. We observed that beta III tubulin is present in the extensions innerving the row of inner hair cells at E18. At E19, the extensions innerving the inner hair cells and the two first rows of outer hair cells were immunolabelled. From E21 to P25, all of hair cells were connected to the spiral ganglion. In the latter, the intensity of immunolabelling decreased between E18 to P25 and the labelling only concerned some cells. These results reveal that beta III tubulin appears before birth in the nervous extensions connecting the sensory cells of the OC according to a modiolar-to-striolar gradient. In the spiral ganglia, the labelling progressively decreases during its development. [less ▲]

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See detailhCG: a pregnancy-related hormone stimulating angiogenesis and pericyte recruitment
Berndt, S; Blacher, Silvia ULg; Perrier d’Hauterive, S et al

Poster (2010)

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