References of "Thiry, Marc"
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See detailImmunoelectron microscopic study of nucleolar DNA during mitosis in Ehrlich tumor cells.
Thiry, Marc ULg; Scheer, U.; Goessens, Guy ULg

in European Journal of Cell Biology (1988), 47(2), 346-57

In order to investigate the DNA localization within Ehrlich tumor cell nucleoli during mitosis, two recent immunocytochemical methods using either an anti-DNA or an anti-bromodeoxyuridine (BrdU ... [more ▼]

In order to investigate the DNA localization within Ehrlich tumor cell nucleoli during mitosis, two recent immunocytochemical methods using either an anti-DNA or an anti-bromodeoxyuridine (BrdU) monoclonal antibody have been applied. In both cases, the immunogold labeling has been performed on ultrathin sections of cells embedded either in Lowicryl K4M or in Epon, respectively. Identical results are observed with both immunocytochemical approaches. In the interphase nucleolus, besides the labeling of the perinucleolar chromatin shell and of its intranucleolar invaginations which penetrate into the nucleolar body and often terminate at the fibrillar centers, a few gold particles are also preferentially found towards the peripheral region of the fibrillar centers. In contrast, the dense fibrillar component and the granular component are never labeled. During mitosis, the fibrillar centers persist at the chromosomal nucleolus organizing regions (NOR's) and can be selectively stained by the silver method. However, these metaphase fibrillar centers are no longer decorated by the DNA- or BrdU antibodies. These results indicate that until the end of prophase, rRNA genes are present inside the fibrillar center material, disappear during metaphase and reappear in reconstituting nucleoli during telophase. Thus, fibrillar centers appear to represent structures sui generis, which are populated by rRNA genes only when the nucleolus is functionally active. In segregated nucleoli after actinomycin D treatment, the DNA labeling is exclusively restricted to the perinucleolar chromatin blocks. These findings also suggest that the DNA content of the fibrillar center material varies according to the rRNA transcription level of the cells. The results are discussed in the light of the present knowledge of the functional organization of the nucleolus. [less ▲]

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See detailEtude cytochimique et immunocytochimique du nucléole
Thiry, Marc ULg

Scientific conference (1987)

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See detailUltrastructure of nucleolus organizing regions in Ehrlich tumour cells
Thiry, Marc ULg

in Biology of the Cell (1987), 60

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See detailUltrastructural and cytochemical study on nucleolus in rat oocytes using RNase gold complex
Antoine, Nadine ULg; Thiry, Marc ULg; Baeckeland, E et al

in Biology of the Cell (1987), 60

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See detailAnti SSA and anti SSB autoantibodies: an immunofluorescence, ultrastructural and immunoblotting study
Thiry, Marc ULg; Vigneron, Alain; Humbel, René et al

Poster (1987)

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See detailLe nucléole : un organite cellulaire fondamental
Lepoint, Alain; Goessens, Guy ULg; Thiry, Marc ULg

in Revue de l'Association Belge des Technologies de Laboratoire (1987), 14

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See detailRelations between nucleoli and nucleolus-organizing regions during the cell cycle
Goessens, Guy ULg; Thiry, Marc ULg; Lepoint, Alain

in Chromosomes Today (1987), 9

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See detailUltrastructural and cytochemical study of nucleoli of Ehrlich tumour cell using a RNase gold complex
Thiry, Marc ULg

in Micron and Microsc. Acta (1987), 18

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See detailBehaviour of nucleolus during mitosis. A comparative ultrastructural study of various cancerous cell lines using the Ag-NOR staining procedure.
Ploton, D.; Thiry, Marc ULg; Menager, M. et al

in Chromosoma (1987), 95(2), 95-107

The aim of the present work was to study the distribution and the behaviour of the silver-staining nucleolar organizer region (Ag-NOR) proteins at the ultrastructural level during interphase and mitosis ... [more ▼]

The aim of the present work was to study the distribution and the behaviour of the silver-staining nucleolar organizer region (Ag-NOR) proteins at the ultrastructural level during interphase and mitosis in five human and murine cancerous cell lines each characterized by a typical nucleolar morphology. During interphase the Ag-NOR proteins are restricted to the fibrillar centres (F.C.) and/or to the dense fibrillar component (D.F.C.). During prophase the silver-staining components come into close contact with some chromosomes and are arranged with a typical polarity: chromosome, F.C. and D.F.C. Then F.C. and D.F.C. together form roundish silver-stained structures and integrate in part within indentations at the periphery of the metaphase chromosomes. During anaphase and telophase large and small spherical silver-staining structures may be seen. They correspond respectively to the metaphase NORs and to numerous structures which appear de novo within ribonucleoprotein (RNP) material localized between the chromosomes. During late telophase the number of the small silver-staining structures decreases whereas the size of the larger ones increases. Then the interphase nucleoli recover their typical shape. These results suggest that when rRNA synthesis is impaired during mitosis the inactive NORs assume a structure and a localization which are not typical of the cell line. In contrast the F.C. and D.F.C. are probably two aspects of the NORs whose typical distribution, relative to the other nucleolar components, gives the interphasic nucleolus its characteristic morphology. [less ▲]

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See detailUltrastructural localisation of AG NOR proteins during interphase and mitosis in human or mouse cancerous cells
Ploton, Dominique; Thiry, Marc ULg; Menager, Monique et al

in Acta Biologica Hungarica (1986), 37

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See detailEssais de localisation des RNA polymérases dans des cellules tumorales d'Ehrlich par immunocytochimie ultrastructurale
Thiry, Marc ULg; Hendrick, J C; Lepoint, Alain et al

in Biology of the Cell (1986), 57

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See detailEtude immunocytochimique ultrastructurale de cellules tumorales d'Ehrlich à l'aide d'anticorps dirigés contre la RNA polymérase d'E. Coli
Thiry, Marc ULg; Hendrick, J C; Lepoint, Alain et al

in Bulletin de l'Association des Anatomistes (1986), 70

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See detailAntinucleolar autoantibodies: ultrastructural localization of antigens on HEp 2 cells
Thiry, Marc ULg; Vigneron, Alain; Humbel, René L et al

Poster (1986)

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See detailUltrastructural localization of antigens on HEp 2 cells using antinucleolar autoantibodies
Thiry, Marc ULg; Vigneron, Alain; Humbel, René L et al

in Immunobiology (1986), 173

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See detailUltrastructural study of nucleolar organizing regions by means of serial sections in Ehrlich tumour cells
Thiry, Marc ULg

in Bulletin de la Société Royale des Sciences de Liège (1986), 4

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See detailUltrastructure of HEp 2 cells
Thiry, Marc ULg; Humbel, R; Goessens, Guy ULg

in Peeters, H (Ed.) Protides of the Biological Fluids (1986)

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