References of "Thiry, Marc"
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See detailImmunoelectron microscope localization of DNase I sensitive sequences within the cell nucleus
Thiry, Marc ULg

in Cell Biology International Reports (1990), 14

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See detailLocalization of DNase I sensitive sequences in specific regions of nuclear architecture
Thiry, Marc ULg

in Archives Internationales de Physiologie et de Biochimie (1990), 98

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See detailComputerized three dimensional visualization of silver stained nucleolar organizer regions studied by high voltage electron microscopy
Ploton, Dominique; Beorchia, A; Ménager, Monique et al

in Biology of the Cell (1990), 69

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See detailNick translation : un outil pour la microscopie électronique
Thiry, Marc ULg

Scientific conference (1990)

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See detailElectron microscope localization of ribosomal RNA and DNA after in situ hybridization
Thiry, Marc ULg; Thiry-Blaise, Lydia

in Harris, J R; Zbarsky, I B (Eds.) Nuclear structure and function (1990)

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See detailSpatial distribution of DNA and histones within Ehrlich tumor cell nucleoli by immunoelectron microscopy
Thiry, Marc ULg

in Harris, J R; Zbarsky, I B (Eds.) Nuclear structure and function (1990)

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See detailUltrastructural cytochemistry of the mammalian cell nucleolus.
Derenzini, Massimo; Thiry, Marc ULg; Goessens, Guy ULg

in Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society (1990), 38(9), 1237-56

In the present review on the organization of the mammalian cell nucleolus, we report and discuss data obtained during the past 10 years by means of cytochemical and immunocytochemical ultrastructural ... [more ▼]

In the present review on the organization of the mammalian cell nucleolus, we report and discuss data obtained during the past 10 years by means of cytochemical and immunocytochemical ultrastructural techniques. Particular emphasis is placed on the following topics: location of the nucleolus organizer regions in interphasic nucleolar components, structure of nucleolar chromatin in situ, and the structure-function relationship of the nucleolar components. The cytochemical and immunocytochemical results are compared and the concordant data are stressed for each topic. [less ▲]

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See detailUltrastructural and cytochemical studies on extranucleolar bodies in rat oocytes at the preovulatory follicle stage.
Antoine, Nadine ULg; Thiry, Marc ULg; Goessens, Guy ULg

in Biology of the Cell (1989), 65(1), 61-6

At the antral follicle stage, the nucleolus is entirely composed of a homogeneous proteinic compact mass. This nucleolar compaction during oogenesis seems to be a general feature in mammalian oocytes ... [more ▼]

At the antral follicle stage, the nucleolus is entirely composed of a homogeneous proteinic compact mass. This nucleolar compaction during oogenesis seems to be a general feature in mammalian oocytes. However, when oocyte maturation is induced by gonadotropin hormone (LH), oocytes enter into preovulatory stage. All the nucleoli are vacuolated and extranucleolar bodies appear in the germinal vesicle near the nucleolar mass. Based on the results obtained by ultrastructural cytochemical stainings, we postulate that these extranucleolar bodies originate from the nucleolar mass itself. The presence of the extranucleolar bodies could reflect the extrusion of nucleolar material, essentially ribonucleoproteins, into the ooplasm. This material could persist after fertilization in the pronuclei until the resumption of transcription at the early stage of embryogenesis. [less ▲]

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See detailIn situ hybridization at the electron microscope level: an improved method for precise localization of ribosomal DNA and RNA.
Thiry, Marc ULg; Thiry-Blaise, L.

in European Journal of Cell Biology (1989), 50(1), 235-43

In situ hybridization using biotinylated rDNA probes and secondary antibody coupled to gold particles was developed on ultrathin sections of Lowicryl-embedded Ehrlich tumor cells for precise localization ... [more ▼]

In situ hybridization using biotinylated rDNA probes and secondary antibody coupled to gold particles was developed on ultrathin sections of Lowicryl-embedded Ehrlich tumor cells for precise localization of ribosomal RNA (rRNA) and ribosomal DNA (rDNA). For the detection of rDNA, an immunocytochemical approach involving an antibody against single-stranded DNA was used in order to determine the more efficient denaturation procedure. Using this technique, rDNA can be visualized in the fibrillar centers of nucleoli, especially in their peripheral regions at the proximity of both the dense fibrils and the nucleolar interstices as well as within the latter. rDNA was occasionally detected in some clumps of dense nucleolus-associated chromatin. Besides the presence of rRNA in the ribosome-rich cytoplasmic areas and in the dense fibrillar component and the granular component of the nucleolus, rRNA was also found in the fibrillar center areas close to the boundary region to the dense fibrillar component. These results are discussed in the light of the present knowledge on the functional organization of the nucleolus. [less ▲]

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See detailUltrastructural distribution of histones within Ehrlich tumor cell nucleoli: a cytochemical and immunocytochemical study.
Thiry, Marc ULg; Muller, S.

in Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society (1989), 37(6), 853-62

We investigated the ultrastructural distribution of histones within Ehrlich tumor cell nucleoli by means of three cytochemical methods and by a Lowicryl post-embedding immunogold labeling procedure ... [more ▼]

We investigated the ultrastructural distribution of histones within Ehrlich tumor cell nucleoli by means of three cytochemical methods and by a Lowicryl post-embedding immunogold labeling procedure involving anti-histone (H2B, H3, H4) antisera as well as antibodies to synthetic peptides of histones. With the two technical approaches, labeling was particularly concentrated over the perinucleolar chromatin and over its intranucleolar invaginations which penetrate the nucleolar body and come in close contact with the fibrillar centers. Furthermore, the high-resolution immunocytochemical technique revealed the presence of a small amount of the three histones in the fibrillar centers, preferentially located towards their peripheral regions. In addition, colocalization of DNA at all the histone-positive sites could be visualized after double immunogold staining using a monoclonal anti-DNA antibody and the anti-histone antisera. These results appear to indicate that all the DNA detected within the nucleolus was associated with histones. This finding suggests that the ribosomal DNA, including transcriptionally active genes, is bound to histones. [less ▲]

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See detailReactivity of autoantibodies in systemic lupus erythematosus with synthetic core histone peptides.
Muller, S.; Bonnier, D.; Thiry, Marc ULg et al

in International Archives of Allergy and Applied Immunology (1989), 89(2-3), 288-96

The specificity of autoantibodies present in the serum of 151 patients with systemic lupus erythematosus (SLE) was investigated by ELISA using as antigen individual histones as well as 17 different core ... [more ▼]

The specificity of autoantibodies present in the serum of 151 patients with systemic lupus erythematosus (SLE) was investigated by ELISA using as antigen individual histones as well as 17 different core histone synthetic peptides. Many of the sera reacted with four terminal peptides (residues 1-21 and 130-135 of H3, 1-29 of H4 and 1-25 of H2B) while fewer reacted with internal peptides (residues 65-85 of H2A and 40-55 of H3). Of the 151 SLE sera, 88% reacted with one or more of the six core histone peptides whereas only 57% reacted with one or more of the complete core histone molecules. Antibodies to mononucleosomes from chicken erythrocytes were also prepared in rabbits. The rabbit antisera were tested by ELISA using as antigen chromatin subunits, native and denatured DNA, individual histones and 23 natural and synthetic peptides of histones. The antinucleosome antibodies were found to recognize the same peptide fragments as those recognized by the SLE sera. [less ▲]

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See detailAnti-bromodeoxyuridine monoclonal antibody: an alternative tool for the identification of replicated DNA at the electron microscope level.
Thiry, Marc ULg; Dombrowicz, D.

in Biology of the Cell (1988), 62(1), 99-102

A new method for identifying the replicated DNA at the electron microscope level is described. Cells were first exposed in vitro to 5-bromodeoxyuridine (BUdR) in conjunction with 5-fluorodeoxyuridine ... [more ▼]

A new method for identifying the replicated DNA at the electron microscope level is described. Cells were first exposed in vitro to 5-bromodeoxyuridine (BUdR) in conjunction with 5-fluorodeoxyuridine (FUdR) and BUdR incorporated into DNA was then detected on Lowicryl-embedded sections by immunogold technique using a monoclonal anti-BUdR antibody. After using this method, chromatin and chromosomes are strongly labelled. [less ▲]

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See detailElectron microscopy proves Jo-1 antigen to be predominantly cytoplasmic but also nuclear.
Thiry, Marc ULg; Humbel, R.; Dicato, M. et al

in Biomedicine & Pharmacotherapy (1988), 42(7), 469-71

The Jo-1 antigen is a specific marker for autoimmune myositis with an excellent correlation with associated interstitial lung disease. Using an electron microscopy immunogold technique, we were able to ... [more ▼]

The Jo-1 antigen is a specific marker for autoimmune myositis with an excellent correlation with associated interstitial lung disease. Using an electron microscopy immunogold technique, we were able to show that the antigen was predominantly cytoplasmic. [less ▲]

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