References of "Thiry, Marc"
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See detailF1FO ATP synthase mutants in Chlamydomonas: Stability and oligomycin resistance mediated by atypical Asa7 protein; interaction between chloroplastic and mitochondrial bioenergetics
Lapaille, Marie ULg; Escobar-Ramírez, Adelma; Degand, Hervé et al

in Biochimica et Biophysica Acta (BBA) - Bioenergetics (2010), 1797(Supplement 1), 29

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See detailHuman Papillomavirus Virus-Like particles and NK cell interactions:role of CD16
Renoux, Virginie ULg; Langers Inge; Clémenceau Béatrice et al

in International Immunology (2010), 22(suppl Pt 5), 17

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See detailSPATIO-TEMPORAL LOCALIZATION OF INTERMEDIATE FILAMENTS IN THE ORGAN OF CORTI BETWEEN THE EMBRYONIC DAY 18 (E18) AND THE POST-NATAL DAY 15 (P15) IN RAT
Johnen, Nicolas ULg; Thelen, Nicolas ULg; Malgrange, Brigitte ULg et al

Poster (2009, October 17)

The mammalian auditory organ, the organ of Corti (OC), is composed of mechanosensory hair cells and nonsensory supporting cell types. Based on their morphology and physiology, a least four types of ... [more ▼]

The mammalian auditory organ, the organ of Corti (OC), is composed of mechanosensory hair cells and nonsensory supporting cell types. Based on their morphology and physiology, a least four types of supporting cells can be identified in the OC: inner pillar cell, outer pillar cell, phalangeal cell and Deiter’s cells. The structure of this organ is well reported in adult but its development is still little known. Using antibodies directed against different proteins of intermediate filaments cytoskeleton, we studied the spatial-temporal localization of cytokeratins (typical of epithelial cells) and vimentin (typical of mesenchymal cells) during the differentiation of the OC in rat from the embryonic day 18 (E18) to the postnatal day (P15). Whatever the antibody used, we observed an obvious labelling over the supporting cells after the birth. In particular, an intense labelling is observed in the pillar cells and in the Deiters’ cells at P8 and at P10. These results suggest that the epithelial-mesenchymal transition might be implicated in the opening of Corti’s tunnel between the pillar cells and the formation of the Nuel’s spaces between the Deiters’ cell and their outer hair cells, at P8 and at P10 respectively. [less ▲]

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See detailSupporting cell cytoskeleton during development of the organ of Corti in rat
Johnen, Nicolas ULg; Thelen, Nicolas ULg; Malgrange, Brigitte ULg et al

Poster (2009, May 11)

The mammalian auditory organ, the organ of Corti (OC), is composed of mechanosensory hair cells and nonsensory supporting cell types. Based on their morphology and physiology, a least four types of ... [more ▼]

The mammalian auditory organ, the organ of Corti (OC), is composed of mechanosensory hair cells and nonsensory supporting cell types. Based on their morphology and physiology, a least four types of supporting cells can be identified in the OC: inner pillar cell, outer pillar cell, phalangeal cell and Deiter’s cells. All supporting cells are highly specialized cells that are characterized by the presence of bundled microtubules with 15 protofilaments instead of 13. Using antibobies against different proteins of cytoskeleton (tubulin, cutokeratin and vimentin), we investigated by confocal microscopy the setting up of supporting cells' cytoskeleton during the differentiation of the OC in art from the embryonic day 18 (E18) to the postnatal 15 (P15). We showed that the inner pillar cells are labelled with an anti-beta IV tubulin from P0. Using an antibody to cytokeratin, a labelling appeared in Deiters' cells from E22. We also revealed that during the development of the OC, supporting cells were labelled with an anti-vimentin antibody from P0. [less ▲]

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See detailInnate lymphocytes in human papillomavirus (HPV)-associated cervical cancers
Renoux, V; Bisig, B; Thiry, Marc ULg et al

Poster (2009)

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See detailMembrane-Type 4 Matrix Metalloproteinase (MT4-MMP) induces lung metastasis by alteration of primary breast tumor vascular architecture
Chabottaux, Vincent; Ricaud, Stéphanie; Host, Lorin et al

in Journal of Cellular & Molecular Medicine (2009)

The present study aims at investigating the mechanism by which MT4-MMP, a membrane-anchored MMP expressed by human breast tumor cells promotes the metastatic dissemination into lung. We applied ... [more ▼]

The present study aims at investigating the mechanism by which MT4-MMP, a membrane-anchored MMP expressed by human breast tumor cells promotes the metastatic dissemination into lung. We applied experimental (intravenous) and spontaneous (subcutaneous) models of lung metastasis using human breast adenocarcinoma MDA-MB-231 cells overexpressing or not MT4-MMP. We found that MT4-MMP does not affect lymph node colonization nor extravasation of cells from the bloodstream, but increases the intravasation step leading to metastasis. Ultrastructural and fluorescent microscopic observations coupled with automatic computer-assisted quantifications revealed that MT4-MMP expression induces blood vessel enlargement and promotes the detachment of mural cells from the vascular tree, thus causing an increased tumor vascular leak. On this basis, we propose that MT4-MMP promotes lung metastasis by disturbing the tumor vessel integrity and thereby facilitating tumor cell intravasation. [less ▲]

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See detailChorionic Gonadotropin Stimulation of Angiogenesis and Pericyte Recruitment
Berndt, Sarah; Blacher, Silvia ULg; PERRIER d'HAUTERIVE, Sophie ULg et al

in Journal of Clinical Endocrinology and Metabolism (2009), 94(11), 4567-74

During the periimplantation period, human chorionic gonadotropin (hCG) plays a key role by increasing the uterine blood flow through uterine vessel vasodilatation but also through angiogenesis. Indeed, we ... [more ▼]

During the periimplantation period, human chorionic gonadotropin (hCG) plays a key role by increasing the uterine blood flow through uterine vessel vasodilatation but also through angiogenesis. Indeed, we previously demonstrated that hCG contributes to endothelial cell recruitment and vessel formation. OBJECTIVE: In this study, hCG was proposed as an arteriogenic factor that could promote perivascular cell recruitment and vessel stabilization. DESIGN: The aortic ring assay, a three-dimensional ex vivo angiogenesis system mimicking all the steps of the angiogenesis process was used to study the impact of hCG on pericyte recruitment and vessel maturation. SETTING: The study was conducted at a university hospital laboratory. MAIN OUTCOME MEASURES: Perivascular cell proliferation, migration, and apposition were quantified by computerized image analysis. RESULTS: Physiological concentrations of hCG (10-400 IU/ml) significantly enhanced pericyte sprouting and migration and gave rise to the maturation and coverage of endothelial capillaries. In a three-dimensional coculture model of endothelial and perivascular cells, hCG enhanced vessel tube formation and endothelial/mural cell adhesion. In addition, hCG stimulated the proliferation of human umbilical vein endothelial cells and smooth muscle cells. The specificity of these effects was determined by using an anti-hCG blocking antibody. Signaling pathways implicated on this hCG effect is protein kinase A and phospholipase C/protein kinase C dependent for the proliferative effect but only phospholipase C/protein kinase C for the migrative process. CONCLUSIONS: Our findings highlight a novel paracrine role of this early embryonic signal in vessel maturation by stimulating perivascular cell recruitment, migration, and proliferation. [less ▲]

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See detailEffects of acriflavine on trypanosomatids: ultrastructural, biochemical and molecular approaches
Manchester, T; Cavalcanti, DP; Simas, C et al

Poster (2009)

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See detail4th International Symposium on Chemosynthesis-based Ecosystems
Ponsard, J; Cambon-Bonavita, M-A; Lepoint, G et al

Poster (2009)

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See detailInnate lymphocytes in human papillomavirus (HPV)-associated cervical cancers
Renoux, V; Bisig, B; Thiry, Marc ULg et al

Poster (2009)

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See detailLocalization of Nopp140 within mammalian cells during interphase and mitosis
Thiry, Marc ULg; Cheutin, Thierry; Lamaye, Françoise et al

Poster (2009)

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See detailTurtle cell cultures for nucleolar studies
Lamaye, Françoise; Thiry, Marc ULg

Poster (2009)

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See detailReptile cell cultures for nucleolar studies
Lamaye, Françoise; Thiry, Marc ULg

Poster (2009)

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See detailVisite guidée au sein du noyau cellulaire
Thiry, Marc ULg

Scientific conference (2009)

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See detailSox10 promotes the survival of cochlear progenitors during the establishment of the organ of Corti
Breuskin, Ingrid ULg; Bodson, Morgan ULg; Thelen, Nicolas ULg et al

in Developmental Biology (2009), 15(335), 327-339

Transcription factors of the SoxE family are critical players that underlie various embryological processes. However, little is known about their function during inner ear development. Here, we show that ... [more ▼]

Transcription factors of the SoxE family are critical players that underlie various embryological processes. However, little is known about their function during inner ear development. Here, we show that Sox10 is initially expressed throughout the otic vesicle epithelium and becomes later restricted to supporting cells as cell differentiation proceeds in the organ of Corti. Morphological analyses of Sox10 mutant mice reveal a significant shortening of the cochlear duct likely resulting from the progressive depletion of cochlear progenitors. While Sox10 appears dispensable for the differentiation and patterning of the inner ear prosensory progenitors, our data support a critical role for this transcription factor in the promotion of their survival. We provide genetic evidences that Sox10, in a concentration-dependant manner, could play a role in the regulation of Jagged1, a gene known to be important for inner ear prosensory development. Together, our results demonstrate that Sox10 regulates the biology of early cochlear progenitors during inner ear development, but, in contrast to neural crest-derived cells, this transcription factor is dispensable for their differentiation. Evidence also suggests that this effect occurs via the activation of the Jagged1 gene. [less ▲]

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See detailConditional knockout of nucleolin in DT40 cells reveals the functional redundancy of its RNA-binding domains.
Storck, Sebastien; Thiry, Marc ULg; Bouvet, Philippe

in Biology of the Cell (2009), 101(3), 153-67

BACKGROUND INFORMATION: Nucleolin is a major nucleolar protein which is highly expressed in rapidly dividing cells and cancer cell lines. This protein is claimed to be multifunctional and could play a ... [more ▼]

BACKGROUND INFORMATION: Nucleolin is a major nucleolar protein which is highly expressed in rapidly dividing cells and cancer cell lines. This protein is claimed to be multifunctional and could play a role in rRNA (ribosomal RNA) synthesis, as well as in cell division or response to cellular stresses. Therefore, how nucleolin influences cell proliferation remained elusive so far. RESULTS: We have generated conditional nucleolin-knockout cells using the chicken B lymphocyte cell line DT40. Our results indicate that nucleolin is absolutely required for the proliferation and for the survival of these cells. Depletion of nucleolin drastically inhibits rDNA (ribosomal DNA) transcription while only slightly affecting pre-rRNA processing. This inhibition is accompanied by modifications of the shape and the structure of the nucleolus. The analysis of mutants of nucleolin, which lack two or three RNA-binding domains, shows that these domains harbour redundant functions and that nucleolin's roles in transcription, rRNA maturation and nucleolar shape can be partially uncoupled. CONCLUSIONS: The function of nucleolin in ribosomal synthesis could account for its effect on cell division and survival, but this vital role does not seem to be linked to sequence-specific RNA binding. [less ▲]

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