Showing results 101 to 120 of 352
 Neonatal development of the auditory organ: the inner pillar cell goes its own wayThelen, Nicolas  ; ; Thiry, Marc Poster (2007) Detailed reference viewed: 7 (0 ULg)Role of Sox 10 in the development of the inner ear; ; Thelen, Nicolas et alPoster (2007) Detailed reference viewed: 7 (0 ULg) The inner pillar cells: an unusual cell type of the auditory organ during neonatal developmentThelen, Nicolas ; Malgrange, Brigitte ; Thiry, Marc Poster (2007) Detailed reference viewed: 1 (0 ULg)A TEM study of the reptilian nucleolus by means of cytochemical and immunocytological methods; Thiry, Marc Poster (2007) Detailed reference viewed: 4 (0 ULg)Ultrastructural organization of the nucleolus in reptiles; Thiry, Marc Poster (2007) Detailed reference viewed: 4 (1 ULg)Cooperation between a tumor protease (MT4-MMP) and a host-derived protease inhibitor (PAI-1) in tumor growth and metastatic dissemination; Thiry, Marc ; et alPoster (2007) Detailed reference viewed: 3 (0 ULg)Ultrastructural organization of the reptilian nucleolus; Thiry, Marc Poster (2007) Detailed reference viewed: 3 (1 ULg) Inactivation of nucleolin leads to nucleolar disruption, cell cycle arrest and defects in centrosome duplication.; ; et al in BMC Molecular Biology (2007), 8 BACKGROUND: Nucleolin is a major component of the nucleolus, but is also found in other cell compartments. This protein is involved in various aspects of ribosome biogenesis from transcription regulation ... [more ▼] BACKGROUND: Nucleolin is a major component of the nucleolus, but is also found in other cell compartments. This protein is involved in various aspects of ribosome biogenesis from transcription regulation to the assembly of pre-ribosomal particles; however, many reports suggest that it could also play an important role in non nucleolar functions. To explore nucleolin function in cell proliferation and cell cycle regulation we used siRNA to down regulate the expression of nucleolin. RESULTS: We found that, in addition to the expected effects on pre-ribosomal RNA accumulation and nucleolar structure, the absence of nucleolin results in a cell growth arrest, accumulation in G2, and an increase of apoptosis. Numerous nuclear alterations, including the presence of micronuclei, multiple nuclei or large nuclei are also observed. In addition, a large number of mitotic cells showed a defect in the control of centrosome duplication, as indicated by the presence of more than 2 centrosomes per cell associated with a multipolar spindle structure in the absence of nucleolin. This phenotype is very similar to that obtained with the inactivation of another nucleolar protein, B23. CONCLUSION: Our findings uncovered a new role for nucleolin in cell division, and highlight the importance of nucleolar proteins for centrosome duplication. [less ▲] Detailed reference viewed: 21 (3 ULg)The nuclear phenotypic plasticity observed in fish during rRNA regulation entails Cajal bodies dynamics.; ; Thiry, Marc et alin Biochemical and Biophysical Research Communications (2007), 360(1), 40-5 Cajal bodies (CBs) are small mobile organelles found throughout the nucleoplasm of animal and plant cells. The dynamics of these organelles involves interactions with the nucleolus. The later has been ... [more ▼] Cajal bodies (CBs) are small mobile organelles found throughout the nucleoplasm of animal and plant cells. The dynamics of these organelles involves interactions with the nucleolus. The later has been found to play a substantial role in the compensatory response that evolved in eurythermal fish to adapt to the cyclic seasonal habitat changes, i.e., temperature and photoperiod. Contrary to being constitutive, rRNA synthesis is dramatically regulated between summer and winter, thus affecting ribosomal biogenesis which plays a central role in the acclimatization process. To examine whether CBs, up to now, never described in fish, were also sustaining the phenotypic plasticity observed in nuclei of fish undergoing seasonal acclimatization, we identified these organelles both, by transmission electronic microscopy and immunodetection with the marker protein p80-coilin. We found transcripts in all tissues analyzed. Furthermore we assessed that p80-coilin gene expression was always higher in summer-acclimatized fish when compared to that adapted to the cold season, indicating that p80-coilin expression is modulated upon seasonal acclimatization. Concurrently, CBs were more frequently found in summer-acclimatized carp which suggests that the organization of CBs is involved in adaptive processes and contribute to the phenotypic plasticity of fish cell nuclei observed concomitantly with profound reprogramming of nucleolar components and regulation of ribosomal rRNAs. [less ▲] Detailed reference viewed: 21 (3 ULg)Tumoral and choroidal vascularization: differential cellular mechanisms involving plasminogen activator inhibitor type I.; Maillard, Catherine ; Lecomte, Julie et alin American Journal of Pathology (2007), 171(4), 1369-80 An adequate balance between serine proteases and their plasminogen activator inhibitor-1 (PAI-1) is critical for pathological angiogenesis. PAI-1 deficiency in mice is associated with impaired choroidal ... [more ▼] An adequate balance between serine proteases and their plasminogen activator inhibitor-1 (PAI-1) is critical for pathological angiogenesis. PAI-1 deficiency in mice is associated with impaired choroidal neovascularization (CNV) and tumoral angiogenesis. In the present work, we demonstrate unexpected differences in the contribution of bone marrow (BM)-derived cells in these two processes regulated by PAI-1. PAI-1(-/-) mice grafted with BM-derived from wild-type mice were able to support laser-induced CNV formation but not skin carcinoma vascularization. Engraftment of irradiated wild-type mice with PAI-1(-/-) BM prevented CNV formation, demonstrating the crucial role of PAI-1 delivered by BM-derived cells. In contrast, the transient infiltration of tumor transplants by local PAI-1-producing host cells rather than by BM cells was sufficient to rescue tumor growth and angiogenesis in PAI-1-deficient mice. These data identify PAI-1 as a molecular determinant of a local permissive soil for tumor angiogenesis. Altogether, the present study demonstrates that different cellular mechanisms contribute to PAI-1-regulated tumoral and CNV. PAI-1 contributes to BM-dependent choroidal vascularization and to BM-independent tumor growth and angiogenesis. [less ▲] Detailed reference viewed: 63 (30 ULg)Plasminogen activator inhibitor type 1 (PAI-1) controls bone marrow-dependent and independent vascularization; ; et al Poster (2006) Detailed reference viewed: 1 (1 ULg)lymphatic ring assay: a new in vitro model of lymphangiogenesis; ; Blacher, Silvia et alPoster (2006) Detailed reference viewed: 6 (0 ULg)The lymphatic ring assay: a new in vitro model of lymphangiogenesis; ; Blacher, Silvia et alConference (2006) Detailed reference viewed: 4 (0 ULg)The lymphatic ring assay: a new in vitro model of lymphangiogenesis; ; Blacher, Silvia et alPoster (2006) Detailed reference viewed: 3 (0 ULg)Role of Sox 10 in the development of the inner ear; ; Thelen, Nicolas et alPoster (2006) Detailed reference viewed: 2 (0 ULg)Sox 10 is not necessary for auditory neurons survival; ; Thelen, Nicolas et alPoster (2006) Detailed reference viewed: 1 (0 ULg)Role of Sox 10 in the development of the inner ear; ; Thelen, Nicolas et alPoster (2006) Detailed reference viewed: 6 (0 ULg)Cooperation between a tumor protease (MT4-MMP) and a host-derived protease inhibitor (PAI-1) in tumor growth and metastatic dissemination; Thiry, Marc ; et alConference (2006) Detailed reference viewed: 1 (0 ULg) |
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