Détermination des besoins spécifiques en acides aminés chez le taurillon Blanc Bleu Belge culard. Rapport d'activité 1996-1998.

Report (1998)

The High Water-Holding Capacity Of Pea Inner Fibers Affects The Ileal Flow Of Endogenous Amino Acids In Pigs

in Journal of Agricultural and Food Chemistry (1998), 46(5), 1927-1934

Pigs were fed protein-free diets containing different pea inner fiber (PIF) isolates (from wrinkled or smooth peas, unprocessed or micronized) or different PIF levels (from 50 to 200 g/kg diet), and the ... [more ▼]

Pigs were fed protein-free diets containing different pea inner fiber (PIF) isolates (from wrinkled or smooth peas, unprocessed or micronized) or different PIF levels (from 50 to 200 g/kg diet), and the flows of endogenous N and amino acids were measured at the ileum level. The flows were better correlated to the water-holding capacity (WHC) of the diet than to fiber intake (R2 = 0.996 vs 0.76, respectively). The relationship with WHC was of the exponential type, with a high increase when the WHC exceeded 3 g of water/g diet. A similar pattern and correlation (R2 = 0.98) was obtained for the ileal flow of nucleobases (markers of epithelial cells and bacteria), whereas the relationships with crude mucus (R2 = 0.96) and diaminopimelic acid (marker of bacteria, R2 = 0.55) were linear. N retention by pigs fed with 0, 30, 60, or 90 g/kg of dry matter (DM) of the same protein isolates did not differ significantly in pigs receiving a 40 or 160 g of PIF/kg diet, despite significant differences in ileal N and amino acid flows between the two fiber levels (2.0 and 3.1 g of N/kg of DM intake, on average). These results and those of the flow of endogenous N compounds suggest an effect of the swollen fibers on the intestinal wall rather than a disturbance of the digestive processes. [less ▲]

Effet des protéines et des fibres alimentaires sur les pertes iléales d'acides aminés endogènes chez le porc.

in Journées de la Recherche Porcine en France (1997), 29

An N-free diet was supplemented with limited but increasing amounts of proteins (30, 60, 90 g/kg diet from a mixture of casein and egg yolk), in order to measure their effect on the ileal endogenous amino ... [more ▼]

An N-free diet was supplemented with limited but increasing amounts of proteins (30, 60, 90 g/kg diet from a mixture of casein and egg yolk), in order to measure their effect on the ileal endogenous amino acid (AA) losses in pigs. The study was conducted at 2 dietary fibre levels: 20 and 80 g/kg, isolated from pea seeds. Compared to the N-free diet, a supply of 30 g proteins was sufficient to obtain a positive N retention and the following supplies allowed a constant and linear increase (0.88 g N retained/g N intake). The same supply induced an increase in ileal endogenous losses, compared to the control (10.7 vs 7.2 g AA/kg DMi for the low fibre diet) but the difference was not significant because of a high variability between the pigs. The supplies of 60 and 90 g proteins did not modify the level of the losses either: 10.2 and 10.6 g AA/kg DMi, respectively. The same trends were observed for the diets with a high fibre level but, in this case, the losses were significantly higher: respectively 14.9, 16.7, 15.7 and 17 g AA/kg DMi. On the other hand, the fraction of the digesta containing the free AA and the small peptides (< 3 kDa), supposed to be mainly of dietary origin, remained independent of the protein level. This suggests a virtually complete digestibility of the dietary proteins. Thus, pea fibres induce a significant increase of the ileal endogenous losses but without disturbing the protein digestion process, as suggested by the N retention. The protein supply probably increased the digestive secretions but the latter could have been highly reabsorbed, due to a lack in dietary factors susceptible to increase the reabsorption. [less ▲]

Influence de la perfusion duodénale de caséinate de sodium sur la rétention azotée chez le taurillon Blanc Bleu Belge culard en croissance et rationné en protéines.

in 4èmes Rencontres autour de la Recherche sur les Ruminants, Paris, France, 4-5 décembre 1997 (1997)

Valeur protéique des matières premières destinées à l'alimentation des porcs: nouvelles perspectives.

Article for general public (1997)

Recherches en production laitière et valorisation du lait

in Annales de Gembloux (1997), 102

Estimation de la fermentescibilité de fourrages et sous-produits tropicaux au moyen du gaz test: étude de quelques facteurs méthodologiques.

in 4èmes Rencontres autour de la Recherche sur les Ruminants, Paris, France, 4-5 décembre 1997 (1997)

Assessment of the 15N-Leucine blood infusion technique for determination of the ileal endogenous N flow and the true ileal amino acid digestibility in pigs.

Conference (1997)

The 13C- vs the 15N-leucine infusion technique for the determination of the ileal endogenous N losses in pigs.

in Lindberg, J. E.; Ogle, Brian (Eds.) Digestive Physiology of Pigs (1997)

Some methodological aspects of the isotope dilution technique using 15N-leucine as a marker of the endogenous N secretions (choice of the precursor pool, time of blood collection, estimation of the ... [more ▼]

Some methodological aspects of the isotope dilution technique using 15N-leucine as a marker of the endogenous N secretions (choice of the precursor pool, time of blood collection, estimation of the isotopique equilibrium) were studied in pigs. Their effect on the estimation of the ileal endogenous N flow was limited. When 15N-leucine was replaced by 13C-leucine, the ileal endogenous N flow obtained with 13C was much lower than that obtained with 15N (1.4 vs 5.7g N/kg DM intake, respectively). The validity of the 13C-leucine infusion technique is questioned. [less ▲]

Prévision de la dégradabilité in situ des matières azotées des fourrages par une méthode enzymatique.

in 3èmes Rencontres autour de la Recherche sur les Ruminants, Paris, France, 4-5 décembre 1996 (1996, December)