References of "Thewis, André"
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See detailBiofuel by-product for poultry diets
Hissette, Mathias ULg; Destain, Jacqueline ULg; Thewis, André ULg et al

Poster (2012, February 28)

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See detailResearch on crude protein and digestibility of Arnica montana L. using conventional NIR spectrometry and hyperspectral imaging NIR
Dale, Laura ULg; Fernandez, Juan Antonio; Vermeulen, Philippe et al

in Journal of Food, Agriculture & Environment (2012), 10(1), 391-396

Arnica montana L. (AM) is considered a medicinal plant, used as hay in feed ration. The aim of this study is to assess the prediction of protein content and in vitro organic matter digestibility value in ... [more ▼]

Arnica montana L. (AM) is considered a medicinal plant, used as hay in feed ration. The aim of this study is to assess the prediction of protein content and in vitro organic matter digestibility value in grass mixtures containing Arnica montana L., and in a second step to check if these values have a positive or negative influence in the mixtures. Crude protein has been selected because it is one of the most important quality parameters of forages as nutritional element used in animal feeding. The protein is required on a daily basis for maintenance, lactation, growth and reproduction, but is important for agriculture too, because a high content of protein makes it an important source of feed. The digestibility is also important, because it refers to the extent to which a feedstuff is absorbed in the animal body as it passes through an animal’s digestive tract. In this study, the Weende system (the Kjeldahl method) for the protein content, together with the enzymatic technique for digestibility, was applied and used in combination with non-destructive methods, like those based on the Near Infrared Spectroscopy (NIRS) or the Near Infrared Hyperspectral Imaging. Based on NIR imaging system data, the PLS-DA was used to discriminate between the classes with AM and classes without AM, as well as to build a model that could be used to predict the composition of mixtures. More than 99% correct prediction for AM was obtained. The crude protein content of the hay determined by classical method decrease from the type of meadow Agrostis capillaris L. - Festuca rubra L. (15.22%) until to the pure sample of Arnica montana L. (11.19%); however, the digestibility was highest in the pure sample of Arnica montana L. (84.13%) and lowest in samples from the type of meadow Agrostis capillaris L. - Festuca rubra L. (57.18%) or in samples with the participation of Arnica montana L. This study should lead to a more important point, which is to verify whether the medicinal properties of Arnica montana L. can be transferred or not to milk production through the dairy cow feed. [less ▲]

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See detailPure species of grass discrimination with the help of hyperspectral imaging NIR
Dale, Laura ULg; Rotar, Ioan; Bogdan, Anca Dorina et al

in Agronomy Series of Scientific Research = Lucrari Stiintifice Seria Agronomie (2011, December), 54(2), 23-27

The possibilities to discriminate with hyperspectral imaging system, SWIR ImSpector N25E in grass mixtures of the pure species Festuca rubra L., Trifolium repens L., Agrostis capillaris L., Hieracium ... [more ▼]

The possibilities to discriminate with hyperspectral imaging system, SWIR ImSpector N25E in grass mixtures of the pure species Festuca rubra L., Trifolium repens L., Agrostis capillaris L., Hieracium aurantiacum L., Arnica montana L.was the objective of this study. All the samples were collected from natural meadows, from the National Apuseni Park, Apuseni Mountains, from Gârda area. The samples were naturally dried then prepared using the protocol for NIRS analyze. The model built with PLS–DA, was used to demonstrate wether the classes discrimination between pure species is possible or not. The goal of this study was to see in other images if the pure species are or are not recognized according to the spectral data base. The potential of using the Hyperspectral Imaging NIR (Camera NIR) to discriminate or to identify pure species was confirmed. For this technique the MatLab program was used .A percentage of more than 96% correct prediction for species discrimination was obtained. This study should drive to a more important point, which is to verify whether the toxic species are or are not used as food for animals in the natural meadows. The floristic composition of a meadow can be determined only if we have in the data base, dates for each identified species, as being part of the mix. [less ▲]

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See detailUsefulness of a Vancomycin pretreatment when challenging chickens in order to evaluate anti-Salmonella preparations
Marcq, Christopher ULg; Cambier, Carole ULg; Thewis, André ULg et al

in Ceylan, Necmettin; Ciftci, Ibrahim; Adabi, Shahram (Eds.) 18th European Symposium on Poultry Nutrition: Proceedings (2011, October)

For many years, Salmonella infection models have been developed in chickens with the aim to study the effects of prophylactic or therapeutic measures on the colonization of the gut. However, although the ... [more ▼]

For many years, Salmonella infection models have been developed in chickens with the aim to study the effects of prophylactic or therapeutic measures on the colonization of the gut. However, although the literature includes numerous challenge models, few studies investigated the infection rates among the inoculated population. We have implemented an antibiotic pretreatment of the chickens (vancomycin hydrochloride, 25 mg/bird) as an infection promoter. Indeed, vancomycin affects the normal gut microflora and releases sites for Salmonella at the intestinal epithelium. Two experiments were undertaken and a presence/absence cloacal swab method was used to evaluate cecal colonization. In the first experiment, birds were orally inoculated with Salmonella Typhimurium at 21 days of age. Three inoculum doses (3 × 103, 3 × 106, 3 × 109 cfu/bird) and an uninfected control were compared according to whether or not vancomycin had been used. Higher levels of Salmonella colonization (more than 70 %) were achieved in the gut by pretreating birds with vancomycin before inoculation (P < 0.05). In the second experiment, chicks were inoculated at 7 days of age with 108 cfu/bird after a vancomycin pretreatment, leading to an infection rate of 87.5 %. In conclusion, vancomycin promotes efficiently the percentage of colonized birds in the challenged population, with either young animals or olders. [less ▲]

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See detailResearch on Crude Protein Contents in Medicago Sativa Hay Harvest During 2008-2009 Using FT-NIR Spectrometry
Dale, Laura ULg; Thewis, André ULg; Rotar, Ioan et al

in Bulletin UASVM Agriculture (2011, September 29)

Medicago sativa or Alfalfa is a flowering plant that belongs to Pea Family that is widely grown throughout the world as forage for cattle, and is most often harvested as hay. Medicago Sativa usually has ... [more ▼]

Medicago sativa or Alfalfa is a flowering plant that belongs to Pea Family that is widely grown throughout the world as forage for cattle, and is most often harvested as hay. Medicago Sativa usually has the highest nutritive value of all common hay crops. This work aims to highlight a way for direct, non-destructive analysis of crude protein content in Medicago sativa hays. The primary objective was to build a model for crude protein calibration for Medicago sativa based on FT-NIR spectroscopy. The samples for analysis were collected over two experimental years (2008-2009) from field trials from the research station– Agricultural Development, Jucu. In order to construct the model, references values are needed; for this reason, crude protein content was determined using the classical Kjeldahl method (Kjeltec Auto Analyser, Tecator). The values for crude protein ranged from 12.63% to 19.12% on the dry matter basis. The regression models build was based on Partial Least Squares (PLS) calculated with the SIMPLS algorithm, using different pre-processing techniques and leave-one-out cross validation. Calibration of the two years together drove to a coefficient of determination for cross validation,R2, of 0.965. The robustness of the model was confirmed by applying it to independent samples (external validation) where the coefficient of determination was R2 = 0.977, RMSEP = 0.8. The results obtained indicated that NIRS can be used to determine crude protein, which could be used as a criteria for quality control of Medicago sativa hays. [less ▲]

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See detailShifts in microbial population fermenting contrasting indigestible carbohydrate sources measured in the intestines of pigs and using an in vitro model of the pig gastro-intestinal tract
Boudry, Christelle ULg; Bindelle, Jérôme ULg; Portetelle, Daniel ULg et al

in International Scientific Conference on probiotics and prebiotics - IPC 2011 Conference Proceedings (2011, June)

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See detailEx vivo ruminal cellulosome for by-product biomass conversion
Hissette, Mathias ULg; Destain, Jacqueline ULg; Thewis, André ULg et al

Conference (2011, April 27)

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See detailIn vitro antagonistic activity evaluation of Lactic Acid Bacteria (LAB) combined with cellulase enzyme against Campylobacter jejuni growth in co-culture
Dubois Dauphin, Robin ULg; vandeplas, Sabrina; Didderen, Isabelle et al

in Journal of Microbiology and Biotechnology (2011), 21(1), 62-70

The antibacterial effects of nine Lactic Acid Bacteria (LAB) on Campylobacter jejuni were investigated using agar gel diffusion and co-culture assay. Inhibition potential was not the same between both ... [more ▼]

The antibacterial effects of nine Lactic Acid Bacteria (LAB) on Campylobacter jejuni were investigated using agar gel diffusion and co-culture assay. Inhibition potential was not the same between both techniques. Only two LAB, Lb. pentosus CWBI B78 and E. faecium THT, showed an anti-campylobacter activity in co-culture assay in using dehydrated poultry excreta mixed with ground straw (DPE/GS) as the only growth substrate source. It was observed that the complementation with Cellulase A complex (Beldem S.A.) of this medium enhanced antimicrobial effect of both bacteria. The co-culture medium acidification was correlated with the concentration in supplemented enzyme. The antibacterial effect was characterized by the production of lactic acid by the homofermentative E. faecium THT and the lactic and acetic acids production by the heterofermentative Lb. pentosus CWBI B78. The antagonistic properties from bacteria-enzyme cooperation could reduce the prevalence of Campylobacter consequently the risk of human infection. [less ▲]

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