Initial rate kinetic study of the Pyruvate Translocator in Intact Rat-heart Mitochondria
Sluse, Francis ; Duyckaerts, Claire ; et al
in Westerhoff, H. V. (Ed.) Biothermokinetics (1993)Detailed reference viewed: 11 (1 ULg)
Biochemical, bioenergetic and ultrastructural survey of the adaptations induced in a skeletal muscle by a chronic electrical stimulation and its cessation
; Sluse, Francis ; et al
in Carraro, U.; Salmons, S. (Eds.) Basic and applied myology : Perspectives for the 90's (1992)Detailed reference viewed: 14 (2 ULg)
Biochemical, genetic and molecular characterization of new respiratory-deficient mutants in Chlamydomonas reinhardtii.
; ; et al
in Plant Molecular Biology (1992), 18
Eight respiratory-deficient mutants of Chlamydomonas reinhardtii have been isolated after mutagenic treatment with acriflavine or ethidium bromide. They are characterized by their inability to grow or ... [more ▼]
Eight respiratory-deficient mutants of Chlamydomonas reinhardtii have been isolated after mutagenic treatment with acriflavine or ethidium bromide. They are characterized by their inability to grow or their very reduced growth under heterotrophic conditions. One mutation (Class III) is of nuclear origin whereas the seven remaining mutants (Classes I and II) display a predominantly paternal mt- inheritance, typical of mutations residing in the mitochondrial DNA. Biochemical analysis has shown that all mutants are deficient in the cyanide-sensitive cytochrome pathway of the respiration whereas the alternative pathway is still functional. Measurements of complexes II + III (antimycin-sensitive succinate-cytochrome c oxido-reductase) and complex IV (cytochrome c oxidase) activities allowed to conclude that six mutations have to be localized in the mitochondrial apocytochrome b (COB) gene, one in the mitochondrial cytochrome oxidase subunit I (COI) gene and one in a nuclear gene encoding a component of the cytochrome oxidase complex. By using specific probes, we have moreover demonstrated that five mutants (Class II mutants) contain mitochondrial DNA molecules deleted in the terminal end containing the COB gene and the telomeric region; they also possess dimeric molecules resulting from end-to-end junctions of deleted monomers. The two other mitochondrial mutants (Class I) have no detectable gross alteration. Class I and Class II mutants can also be distinguished by the pattern of transmission of the mutation in crosses. An in vivo staining test has been developed to identify rapidly the mutants impaired in cyanide-sensitive respiration. [less ▲]Detailed reference viewed: 17 (1 ULg)
Study of a new presumed mitochondrial carrier, the product of the yeast RIM 2 gene
; ; Duyckaerts, Claire et al
in Archives Internationales de Physiologie, de Biochimie et de Biophysique (1992), 100Detailed reference viewed: 15 (1 ULg)
Influence of tension reduction and peripheral dissection on histologic, biochemical and bioenergetic profiles, and kinetics of skeletal muscle fast-to-slow transformation.
; Sluse, Francis ; et al
in Journal of Cardiac Surgery (1991), 6(1), 195-206Detailed reference viewed: 5 (3 ULg)
Kinetic study of the aspartate/glutamate carrier in intact rat heart mitochondria and comparison with a reconstituted system.
Sluse, Francis ; ; et al
in Biochimica et Biophysica Acta-Bioenergetics (1991), 1058
The homologous exchange of external [14C] aspartate/internal aspartate catalyzed by the aspartate/glutamate carrier of rat heart mitochondria was investigated using aspartate-loaded, glutamate-depleted ... [more ▼]
The homologous exchange of external [14C] aspartate/internal aspartate catalyzed by the aspartate/glutamate carrier of rat heart mitochondria was investigated using aspartate-loaded, glutamate-depleted mitochondria. An inhibitor-stop technique was developed for kinetic studies by applying pyridoxal phosphate. Direct initial rate determinations from the linear phase of [14C] aspartate uptake were insufficiently accurate at high external and/or low internal substrate concentrations. Therefore, the full time-course of [14C] aspartate uptake until reaching isotope equilibrium was fitted by a single exponential function and was used to calculate reliable initial steady-state rates. This method was applied in bisubstrate analyses of the antiport reaction for different external and internal aspartate concentrations. The kinetic patterns obtained in double reciprocal plots showed straight lines converging on the abscissa. This result is consistent with a sequential antiport mechanism. It implies the existence of a catalytic ternary complex that is formed by the translocator and substrate molecules bound from both sides of the membrane. The Km values for aspartate were clearly different for the external and the internal sides of the membrane, 216 +/- 23 microM and 2.4 +/- 0.5 mM, respectively. These values indicated a definite transmembrane asymmetry of the carrier. The same asymmetry became evident when investigating the isolated protein from bovine heart mitochondria after reconstitution into liposomes. In this case the Km values for external and internal aspartate were determined to be 123 +/- 11 microM and 2.8 +/- 0.6 mM, respectively. This comparison demonstrates a right-side out orientation of the carrier after insertion into liposomal membranes. The sequential transport mechanism of the aspartate/glutamate carrier, elucidated both in proteoliposomes and in mitochondria, also seems to be a common characteristic of other mitochondrial antiport carriers [less ▲]Detailed reference viewed: 9 (2 ULg)
Tocopherol mobilization during dynamic exercise after beta-adrenergic blockade
; Pincemail, Joël ; Roesgen, Alice et al
in Archives Internationales de Physiologie et de Biochimie (1990), 98Detailed reference viewed: 8 (1 ULg)
Iron status in runners of various running specialities
; Sluse, Francis ; et al
in Archives Internationales de Physiologie et de Biochimie (1990), 98Detailed reference viewed: 8 (2 ULg)
Kinetic mechanism of mitochondria carriers catalysing exchange reactions
Sluse, Francis ; ; Duyckaerts, Claire et al
in Journal of Biosciences (1990), 15Detailed reference viewed: 6 (4 ULg)
Induction and Characterization of Mitochondrial DNA Mutants in Chlamydomonas Reinhardtii
Matagne, René-Fernand ; ; Munaut, Carine et al
in Journal of Cell Biology (1989), 108(4), 1221-6
In addition to lethal minute colony mutations which correspond to loss of mitochondrial DNA, acriflavin induces in Chlamydomonas reinhardtii a low percentage of cells that grow in the light but do not ... [more ▼]
In addition to lethal minute colony mutations which correspond to loss of mitochondrial DNA, acriflavin induces in Chlamydomonas reinhardtii a low percentage of cells that grow in the light but do not divide under heterotrophic conditions. Two such obligate photoautotrophic mutants were shown to lack the cyanide-sensitive cytochrome pathway of the respiration and to have a reduced cytochrome c oxidase activity. In crosses to wild type, the mutations are transmitted almost exclusively from the mating type minus parent. A same pattern of inheritance is seen for the mitochondrial DNA in crosses between the two interfertile species C. reinhardtii and Chlamydomonas smithii. Both mutants have a deletion in the region of the mitochondrial DNA containing the apocytochrome b gene and possibly the unidentified URFx gene. [less ▲]Detailed reference viewed: 24 (3 ULg)
Bioenergetic peculiarity of heart mitochondria from the hemoglobin-and myoglobin-free antartic icefish
Feller, Georges ; Gerday, Charles ; et al
in Biochimica et Biophysica Acta-Bioenergetics (1989), 977Detailed reference viewed: 17 (13 ULg)
Kinetic mechanism of the adenylic and the oxoglutaruc carriers : a comparison
Sluse, Francis ; ; Duyckaerts, Claire
in Azzi, A.; Nalecz, M. J.; Wojtczak, L. (Eds.) Anion carriers of mitochondrial membranes (1989)Detailed reference viewed: 9 (1 ULg)
Spontaneous modification of the oxoglutarate translocator in vivo.
Duyckaerts, Claire ; ; Sluse, Francis et al
in European Journal of Biochemistry (1984), 142Detailed reference viewed: 9 (2 ULg)
Conformational changes and possible structure of the oxoglutarate translocator of rat-heart mitochondria revealed by the kinetic study of malate and oxoglutarate uptake.
; Sluse, Francis ; Duyckaerts, Claire et al
in European Journal of Biochemistry (1983), 134Detailed reference viewed: 8 (1 ULg)
La multiplicite enzymatique: aspects structurels, genetiques, phyllogeniques, ontogeniques, metaboliques et pathologiques.
in Revue Médicale de Liège (1982), 37(7), 242-50Detailed reference viewed: 7 (4 ULg)
Cinétique du transporteur oxoglutarate des mitochondries de coeur de rat198
Thèse d’agrégation de l’enseignement supérieur (1981)Detailed reference viewed: 15 (4 ULg)
Kinetic mechanism of the exchanges catalysed by the adenine-nucleotide carrier.
Duyckaerts, Claire ; ; et al
in European Journal of Biochemistry (1980), 106Detailed reference viewed: 11 (1 ULg)
Oxoglutarate translocator of rat-heart mitochondria: regulation by aspartate.
Sluse, Francis ; Duyckaerts, Claire ; et al
in FEBS Letters (1980), 120Detailed reference viewed: 4 (0 ULg)