References of "Serteyn, Didier"
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See detailDo polyamines increase the antioxidant capacity of hyperhydric shoots ?
Franck, Thierry ULg; Tabart, Jessica ULg; Kevers, Claire ULg et al

in 4th international workshop - cost action FA0605 - Book of abstracts - Limassol 17-19 November (2011)

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See detailModulatory activities of Agelanthus dodoneifolius (Loranthaceae) extracts on stimulated equine neutrophils and myeloperoxidase activity
Boly, Rainatou; Dessy, Stephanie; Kohnen, Stephan et al

in International Journal of Molecular Medicine (2011), 28

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See detailAssociation between Myeloperoxidase concentration in equine frozen semen and post thawing parameters
Ponthier, Jérôme ULg; Franck, Thierry ULg; Detilleux, Johann ULg et al

in Reproduction in Domestic Animals (2010), 45(5), 811-816

Despite improvement of techniques, semen of 20% of stallions remains unfreezable. Recent studies focused on impact of reactive oxygen species and oxidant enzymes on semen characteristics. Myeloperoxidase ... [more ▼]

Despite improvement of techniques, semen of 20% of stallions remains unfreezable. Recent studies focused on impact of reactive oxygen species and oxidant enzymes on semen characteristics. Myeloperoxidase (MPO) is a pro-oxidant enzyme contained in and released by neutrophils during degranulation or after cell lysis. It is responsible for the formation of hypochlorous acid, a strong oxidant agent which could damage spermatozoa. The aim of this study was to determine the relation between MPO concentration and characteristics of frozen semen from stallions. Thirty five straws from different stallions were analyzed. Post-thawing spermatozoal concentration, progressive and total motility were determined by CASA. Freezability was determined according to post-thawing progressive motility (over or under 15%). Percent of alive spermatozoa and abnormal forms were determined after Eosin-Nigrosin and Diff-Quick® staining respectively. Post-thawing MPO concentration was measured by ELISA. Our study shows that frozen thawed semen contains large amounts of free MPO. We also observed that post-thawing MPO ELISA assay can be used as an indicator of equine semen freezability. High MPO concentration samples showed lower total and progressive motility. A higher proportion of abnormal head shape associated with acrosome reaction was observed in our late examinations of the high concentration MPO group. Our results show that MPO adversely affects total and progressive motility of equine semen. A negative correlation between normal motile forms and MPO concentration was also observed. The effect of MPO on dead or abnormal forms remains to be precised. [less ▲]

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See detailAntioxidant activity of Passiflora edulis and Passiflora alata fruits
Yariwake, J.; Zeraik, M.; Serteyn, Didier ULg et al

in Planta Medica (2010, September), 76(12), 1274-1275

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See detailCan we predict troubles during horse clinical examination by a simple test?
Peeters, Marie ULg; Godfroid, Sandra; Sulon, Joseph et al

Poster (2010, August 03)

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See detailThe relationship between fetlock joint dorsiflexion and flexor tendon moment arms variations
Noble, Prisca ULg; Collin, Bernard ULg; Denoix, Jean-Marie et al

in Anatomia, Histologia, Embryologia (2010, July 28), 39(4), 335-336

Introduction: To calculate flexor tendon forces on the basis of inverse dynamic analysis, flexor tendon moment arms need to be determinated in the equine distal forelimb. Moment arm of deep digital flexor ... [more ▼]

Introduction: To calculate flexor tendon forces on the basis of inverse dynamic analysis, flexor tendon moment arms need to be determinated in the equine distal forelimb. Moment arm of deep digital flexor tendon (dDDF) and of superficial digital flexor tendon (dSDF) have been recognised to vary during the fetlock joint dorsiflexion, however little distal forelimb model has taken into account dSDF and dDDF variations. This study describes a method to determinate the relationship between the fetlock joint dorsiflexion and the flexor moment arms variations. Methods: Eleven forelimbs of eleven horses (~500 Kg) were collected, transversally cut below the shoulder joint, frozen and cut into a sagittal plane until above the fetlock joint. Considering the forelimb pulley model (Meershoek et al. AJVR 2001; 62 1585-1593), in the segment based coordinate system, for the fetlock joint the pulley centers of the DDF and SDF tendons were determined directly from the section. For the fetlock radius-pulley experiments, forelimbs were thawed overnight, fixed to an immobilizing support and attached to a hoist that dorsiflexed the fetlock joint. For the fetlock dorsal angle (θ) 180° to 150°, the dDDF and the dSDF were measured directly from the section. For each tendon all of these data were fitted using a polynomial regression model (OriginPro8) and the relationships between θ and the radius pulley variation (∆d) was described assuming that ∆d was zero at θ equal to 180°. Results : When the fetlock dorsiflexion increased, dDDF and dSDF decreased (∆dDDF(150)= -6.1 mm; ∆dSDF(150)= -5.2 mm) according to the following equations: ∆dDDF(θ)= -0.24923 x θ1 + 0.00139 x θ² (R²=0.89867) ; ∆dSDF(θ)= -0.21494 x θ1 + 0.0012 x θ² (R²=0.91504). Conclusions : A distal forelimb pulley model with variable dDDF and dSDF has been performed. It could become an important tool for use in inverse dynamic procedures. [less ▲]

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See detailRetrospective evaluation of 9 horses with surgical treatment of keratoma
Salciccia, Alexandra ULg; Bouhmala, Nabil; Serteyn, Didier ULg et al

Poster (2010, July 02)

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See detailEvaluation of a hydro-alcoholic solution as pre-surgical hand antisepsis in a veterinary setting.
Verwilghen, Denis ULg; Mastrocicco, Emilie; Mainil, Jacques ULg et al

Conference (2010, July 02)

Introduction: Despite the fact that presurgical antiseptic hand treatment of surgical staff has since become a worldwide accepted procedure, surgical site infection is still one of the most frequent types ... [more ▼]

Introduction: Despite the fact that presurgical antiseptic hand treatment of surgical staff has since become a worldwide accepted procedure, surgical site infection is still one of the most frequent types of nosocomial infections. Many products have been used for hand antisepsis, but the popularity of alcoholic rubs amongst human surgeons is increasing as they have shown to provide a rapid and immediate action, are considerably faster than disinfecting soap scrubs and cause less skin damage after repeated use. The purpose of this study was 1) to identify surgical hand antisepsis habits amongst veterinary surgery specialists in Europe (ECVS) and the United States (ACVS), 2) to compare povidone iodine and chlorhexidine gluconate scrubs with a hydro-alcoholic rub hand antisepsis protocol and 3) to evaluate the usefulness of a hydro-alcoholic rub solution in a veterinary surgical setting. Materials and Methods: Emails were sent to 1300 Diplomates to invite them to participate to an online survey in order to obtain an idea about pre-surgical hand disinfection techniques. In a preliminary trial the efficiency of 4% chlorhexidine gluconate, 7.5 % PVP-iodine and an alcoholic solution containing 45% 2-propanol, 30% 1-propanol, 0.2% mecetronium ethylsulphate (Sterillium®) in bacterial reduction on hands was compared. In a clinical trial, the suitability of Sterillium® was assessed in an equine and small animal set up during surgery procedures. Fingertips were pressed on blood agar plates and Gassner plates prior to hand antisepsis (PHA), after handantisepsis (AHA) and three hours after wearing sterile gloves (AG) in the preliminary trial or at the end of surgery (AS) in the clinical trial. Bacterial counts (colony forming units : CFU’s) were obtained after 24 h of incubation of the plates. The obtained values of CFU from PHA, AHA, AG and AS were expressed as log10 values. For each sample, a reduction factor (RF) was obtained from the difference of log10 pre-value and log10 post-value. An ANOVA comparison between the effects of the different antisepsis protocols on the mean log10 CFU values and RF’s in function of the different steps was established. Results: A 42.6% response rate was obtained for the survey. Most surgeons’ still use a disinfecting soap only (79.9%) for hand antisepsis prior to surgery, the majority based on chlorhexidine gluconate (81.4%). Significant differences were found between immediate and sustained activities of the different products tested. Sterillium® was shown to have significantly lower LSM log10 CFU at AG compared to both other products. At AHA, povidone iodine revealed to have significantly higher LSM log10 CFU than Sterillium® and chlorhexidine gluconate, with the last two products having comparable activities. Reduction factors for the Sterillium® were significantly greater than for the other products. Only RF1 was comparable between Sterillium® and chlorhexidine gluconate. In the clinical trial, no significant differences were found between surgeons regarding LSM log10 CFU after hand antisepsis, neither between RF from samples taken at the small animal versus the equine surgery theatre. Discussion: This study confirms that Sterillium® is more effective in reducing bacterial counts on hands prior to surgery in a veterinary setting as are chlorhexidine gluconate (CHX) and povidone iodine soap. Moreover, they provide better tolerance and compliance to surgical hygiene protocols. Despite this, veterinary surgeons from all over the world still prefer the use of CHX soap, which has far more disadvantages than commonly accepted. Apart from the better skin tolerance and the absence of known resitance to the product, the use of Sterillium® offers the advantage of a fast (1.5 minute) surgical handantisepsis. This study shows that, as previously reported for human medicine, Sterillium® can safely be used in a veterinary surgical setting. [less ▲]

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See detailAssessment of stress level in horses during competition using salivary cortisol: preliminary studies
Peeters, Marie ULg; Sulon, Joseph; Serteyn, Didier ULg et al

in Journal of Veterinary Behavior : Clinical Applications and Research (2010, July), 5(4), 216

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See detailMeckel's diverticulum as a cause of colic: 2 cases with different morphological features
Verwilghen, Denis ULg; Van Galen, Gaby ULg; Busoni, Valeria ULg et al

in Tijdschrift voor Diergeneeskunde (2010), 135(11), 452-455

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See detailSTEM CELL IN THE TREATMENT OF TENDONITIS IN EQUINE PATIENTS
Verwilghen, Denis ULg; Briquet, Alexandra ULg; Gothot, André ULg et al

Poster (2010, May 07)

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See detailA pendulum test as a tool to evaluate viscous friction parameters in the equine fetlock joint
Noble, Prisca ULg; Lumay, Geoffroy ULg; Coninx, Marc ULg et al

Poster (2010, April)

This study describes an equine fetlock joint pendulum test, and investigates the influence of post mortem time and intra-articular lipid solvent on the viscous frictional response. Fresh equine digits ... [more ▼]

This study describes an equine fetlock joint pendulum test, and investigates the influence of post mortem time and intra-articular lipid solvent on the viscous frictional response. Fresh equine digits (control group 1 (n = 6); lipid solvent group 2 (n = 6)) were mounted in a pendulum tribometer. Assuming that pendular joint damping could be modelled by a harmonic oscillator fluid damping (HOFD), damping time (t), viscous damping coefficient (c ) and friction coefficient (µ) were then followed for 5 hours under experimental conditions (400 N; 20 °C). In all experiments, pendular joint damping was found to follow an exponential decay function (R² = 0.99714), which confirms that joint damping is fluid. The evolution of t, c and µ was found to be significantly (P <0.05) different in the two groups. Indeed, t decreased and c and µ increased more rapidly and strongly in the group 2. It was concluded that pendular joint damping can be modelled by an HOFD model. The influence of post mortem time on results suggests that, ideally, joint mechanical properties should only be tested on fresh cadavers at the same post mortem time. Finally, the addition of lipid solvent was found to be responsible for upper viscous friction parameters and for a reduced damping time, which suggests that articular lubricating ability was compromised. This equine pendulum test could be used to test the efficacy of various bio-lubricant treatments. [less ▲]

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See detailKit for the sequential measurement of (1) the enzymatically active fraction and (2) the total amount of an enzyme
Serteyn, Didier ULg; Deby-Dupont, Ginette; Franck, Thierry ULg et al

Patent (2010)

The present invention is related to a combined kit for sequential measurement of the enzymatically active fraction and total amount of an enzyme [(such as myeloperoxidase (MPO)] in a sample, and that find ... [more ▼]

The present invention is related to a combined kit for sequential measurement of the enzymatically active fraction and total amount of an enzyme [(such as myeloperoxidase (MPO)] in a sample, and that find improved applications in veterinary and human health fields. [less ▲]

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See detailAn equine joint friction test model using a cartilage-on-cartilage arrangement.
Noble, Prisca ULg; Collin, Bernard ULg; Lecomte-Beckers, Jacqueline ULg et al

in Veterinary Journal (2010)

This study describes an equine joint friction test using a cartilage-on-cartilage arrangement and investigates the influence of age and load on the frictional response. Osteochondral plugs were extracted ... [more ▼]

This study describes an equine joint friction test using a cartilage-on-cartilage arrangement and investigates the influence of age and load on the frictional response. Osteochondral plugs were extracted from equine shoulder joints (2-5 years, n=12; 10-14 years, n=15), and mounted in a pin-on-disc tribometer. The frictional response was then measured under constant conditions (2N; 20 degrees C; 5 mm/s), and with increasing load (2N, 5N, 10N). In all experiments, the friction coefficient of young cartilage was significantly (P<0.001) smaller than obtained from old cartilage, while the application of a greater load resulted in a significant (P<0.001) decrease in friction coefficient only in old cartilage. It was concluded that cartilage ageing was responsible for an increase in friction coefficient under these experimental conditions. Moreover, where young cartilage lubrication remained stable, cartilage ageing may have been responsible for lubrication regime change. The cartilage-on-cartilage model could be used to better understand lubrication regime disturbances in healthy and diseased equine joints, and to test the efficacy of various bio-lubricant treatments. [less ▲]

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See detailGene expression profiling from leukocytes of horses affected by osteochondrosis.
Serteyn, Didier ULg; Piquemal, D.; Vander Heyden, Laurent ULg et al

in Journal of Orthopaedic Research (2010)

Osteochondrosis (OC) is a developmental disease that affects growing horses and that severely affects their ability to perform. The genetic basis of its pathogenesis is poorly understood. The aim of the ... [more ▼]

Osteochondrosis (OC) is a developmental disease that affects growing horses and that severely affects their ability to perform. The genetic basis of its pathogenesis is poorly understood. The aim of the study was to analyze the transcript profile of leukocytes from horses affected with OC. Two transcriptome libraries were constructed from leukocytes of OC-affected and non-OC-affected horses using digital gene expression analysis (DGE) and real-time PCR. Statistical analysis allowed selection of 1,008 tags upregulated in the non-OC-affected group and 1,545 tags upregulated in the OC-affected group. Among these genes, 16 regulated genes and 5 housekeeping genes were selected. Metabolic pathways analysis showed an obvious dysregulation of several signaling pathways related to cartilage formation or cartilage repair, including Wnt, Indian hedgehog, and TGF-beta signaling. Other genes, including ISG, ApoB, MGAT4, and TBC1D9, showed a significantly different expression between groups. These genes may play a role in high carbohydrate diet, abnormal insulin metabolism, or inflammation, mechanisms suspected to be involved in OC. This DGE analysis of the transcript profile of leukocytes from OC-affected horses demonstrated significant differences in comparison to the control library. These results open new perspectives for the understanding of equine OC. (c) 2010 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res. [less ▲]

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See detailL'articulation synoviale : un systeme tribologique parfait
Noble, Prisca ULg; Collin, Bernard ULg; Lecomte-Beckers, Jacqueline ULg et al

in Annales de Médecine Vétérinaire (2010), 154(2), 83-93

The synovial joint, by load bearing and by minimal wear and minimal friction conferring, is considered as a perfect tribological system. During these last years, researchs in articular biomechanics have ... [more ▼]

The synovial joint, by load bearing and by minimal wear and minimal friction conferring, is considered as a perfect tribological system. During these last years, researchs in articular biomechanics have been performed to study how the joint friction coefficient, a dimensionless measure which represents the ratio between the frictional force on the normal force, was close to zero. Today, there is still no consensual explanation and the articular prosthesis partially imitates the biological mechanics. This review describes the composition and the general properties of the synovial joint components. Additionally, different theorical models, on the tribology of the articular triplex cartilage-synovial fluid-cartilage, are explained. [less ▲]

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See detailHeel effects on joint contact force components in the equine digit : a sensitivity analysis
Noble, Prisca ULg; Lejeune, Jean-Philippe ULg; Caudron, Isabelle ULg et al

in Equine Veterinary Journal. Supplement (2010), 42

Reasons for performing study: Whereas the effect of heel configuration on the tension of the suspensory apparatus is well documented in the literature, there are few reports of joint contact force ... [more ▼]

Reasons for performing study: Whereas the effect of heel configuration on the tension of the suspensory apparatus is well documented in the literature, there are few reports of joint contact force components in the equine distal forelimb. Objectives: To improve understanding of the effect of heel configuration on equine digit joint loading, a sensitivity analysis was performed to compare the effect of a raised heel on joint contact force components in the coffin and fetlock joints during the stance phase of the trot. Materials and methods: FourWarmblood horses were used. An inverse dynamic analysis was carried out using kinematic and kinetic data. Taking into account the tendon wrapping forces (WF) around the sesamoid bones in the calculations, the joint contact forces (CF) were estimated for the coffin and fetlock joints during the trot stance phase (4 m/s). To test the sensitivity of the results to heel configuration changes, calculations were performed repeatedly for different heel configurations (raised by 0, 6 and 12°). A one-way ANOVA with repeated measures was used to test the effect of heel configuration (at the 3 levels) (a = 0.05; P<0.05; post hoc testing: Bonferroni). Results: For heel configurations raised from 0–12°: whereas the tension of the deep digital flexor tendon decreased and the tension of the superficial digital flexor tendon increased, for the coffin joint the peakWF(1.4 +- 0.25 bwt; 1.2 +- 0.2 bwt; 0.95 +- 0.1 bwt) and the peak CF (2.45 +- 0.25 bwt; 2.2 +- 0.2 bwt; 2 +- 0.1 bwt) decreased significantly (P<0.05). For the fetlock joint, the peak WF (3.8 +- 0.7 bwt; 4.1 +- 0.3 bwt; 4.4 0.25 bwt) and the peak CF (4.35 +- 0.7 bwt; 4.7 +- 0.35 bwt; 5 +- 0.3 bwt) increased, but not significantly. Conclusion: This analysis suggests that the coffin joint loading and fetlock joint loading are strongly connected. The heel configuration may influence both coffin joint and fetlock joint contact force components. [less ▲]

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See detailDevelopment of an enzyme-linked immunosorbent assay for equine neutrophil elastase measurement in blood: Preliminary application to colic cases.
de la Rebière de Pouyade, Geoffroy ULg; Franck, Thierry ULg; Salciccia, Alexandra ULg et al

in Veterinary immunology and immunopathology (2010)

Equine neutrophil elastase (NE) is a protease released in inflammatory diseases and participating in tissue destruction. To measure NE in horse plasma to assess its role in pathological conditions, we ... [more ▼]

Equine neutrophil elastase (NE) is a protease released in inflammatory diseases and participating in tissue destruction. To measure NE in horse plasma to assess its role in pathological conditions, we purified elastase from equine neutrophils by a double step chromatography and obtained a pure protein of 27kDa, 4kDa smaller than the NE 2A previously purified (Scudamore et al., 1993; Dagleish et al., 1999), which was likely to be NE 2B. We developed an ELISA by using two specific polyclonal antibodies obtained from rabbit and guinea pig. The sandwich complex was detected using a secondary antibody conjugated to alkaline phosphatase. The ELISA showed good precision and accuracy, with intra- and inter-assay coefficients of variation below 10% for equine NE concentrations ranging from 1.875 to 60ng/ml. A stable plasma NE value, unaffected by the delay of centrifugation (over 4h), was obtained with plasma from EDTA anticoagulated blood. The mean value (+/-SEM) measured in 37 healthy horses was 32.53+/-4.6ng/ml. NE level in plasma of horses with colic at the time of admission was significantly higher than in healthy horses. Our results indicate that the ELISA technique we developed to measure plasmatic NE is a powerful tool for studying the role of elastase in equine inflammatory disease. In future, the application will be extended to other equine biological fluids. [less ▲]

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See detailLes tendinopathies et desmopathies des régions métacarpienne et –tarsienne: revue des thérapies actuelles. Seconde partie : les traitements
Verwilghen, Denis ULg; Caudron, Isabelle; Van Galen, Gaby ULg et al

in Annales de Médecine Vétérinaire (2010), 154(1), 1-15

Lesions to the horse’s tendon are very frequent and are a non negligible reason for early retirement of sport and leisure horses. For decades man has been searching for “the” treatment but unfortunately ... [more ▼]

Lesions to the horse’s tendon are very frequent and are a non negligible reason for early retirement of sport and leisure horses. For decades man has been searching for “the” treatment but unfortunately most of the known treatments have empiric bases and very few have actually proven their real efficiency. Diagnostic techniques like ultrasound allows to better evaluate the improvements made in tendonitis therapies. Stem cells and gene therapy are probably the most innovating of them, but their long-term effectiveness still has to stand the test of times. This article gives an overview of the possible treatments of tendonitis today. [less ▲]

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