References of "Serteyn, Didier"
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See detailOsteochondosis in foals
Sandersen, Charlotte ULg; Vander Heyden, Laurent ULg; Detilleux, Johann ULg et al

in Veterinary Record : Journal of the British Veterinary Association (2013), 127(17), 456-467

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See detailCurcumin and resveratrol act by different ways on NADPH oxidase activity and reactive oxygen species produced by equine neutrophils
Derochette, Sandrine ULg; Franck, Thierry ULg; Mouithys-Mickalad, Ange ULg et al

in Chemico-Biological Interactions (2013), 206

In neutrophils (PMNs), superoxide anion (O2●-), the first reactive oxygen species (ROS) produced to kill pathogenic agents, is generated by NADPH oxidase, an enzymatic complex formed by the translocation ... [more ▼]

In neutrophils (PMNs), superoxide anion (O2●-), the first reactive oxygen species (ROS) produced to kill pathogenic agents, is generated by NADPH oxidase, an enzymatic complex formed by the translocation of cytosolic subunits to the membrane flavocytochrome b558. In horses, excessive activation of PMNs is often associated with deadly pathologies and the modulation of their ROS production by acting on NADPH oxidase is a prime target to manage inflammation. We developed a cell-free assay to measure the activity of equine NADPH oxidase assembled in vitro, in order to test the effects of natural or synthetic compounds on the enzyme activity or assembly. The cell-free assay was validated with diphenyleneiodonium chloride and Gp91ds-tat, two inhibitors largely described for human NADPH oxidase. The anti-oxidant effects of curcumin and resveratrol at final concentration ranging from 10-4 to 10-6 M were studied on whole cells by chemiluminescence (CL) and by cell-free assay, in which the molecule was added before or after the enzyme assembly. The CL assay demonstrated that curcumin efficiently inhibited the O2●- production and easily entered into PMNs or interacted with their membrane. Cell-free assay showed that curcumin acted on the reconstitution of NADPH oxidase even at 10-5 M, while resveratrol appeared to be an O2●- scavenger rather than an inhibitor of NADPH oxidase activity, since it acted from outside the cell in CL and after the complex assembly in cell-free assay. By acting directly on NADPH oxidase, curcumin should be a good candidate for the treatment of acute or inflammatory diseases involving an excessive ROS production. [less ▲]

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See detailEffect of myeloperoxidase and anoxia/reoxygenation on mitochondrial respiratory function of cultured primary equine skeletal myoblasts.
Ceusters, Justine ULg; Mouithys-Mickalad, Ange ULg; Franck, Thierry ULg et al

in Mitochondrion (2013), 13(5),

Horses are particularly sensitive to excessive inflammatory reaction where myeloperoxidase, a marker of inflammation, may contribute to mitochondrial dysfunctions. This study investigated the interaction ... [more ▼]

Horses are particularly sensitive to excessive inflammatory reaction where myeloperoxidase, a marker of inflammation, may contribute to mitochondrial dysfunctions. This study investigated the interaction between myeloperoxidase and cultured primary equine skeletal myoblasts, particularly its effect on mitochondrial respiration combined or not with anoxia followed by reoxygenation (AR). We showed that active myeloperoxidase entered into the cells, interacted with mitochondria and decreased routine and maximal respirations. When combined with AR, myeloperoxidase caused a further decrease of these respiratory parameters while the leak increased. Our results indicate that myeloperoxidase amplifies the mitochondrial damages initiated by AR phenomenon and alters the mitochondrial function. [less ▲]

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See detailAssociation of breeding conditions with prevalence of osteochondrosis in foals
Vander Heyden, Laurent ULg; Lejeune, Jean-Philippe ULg; Caudron, Isabelle ULg et al

in Veterinary Record : Journal of the British Veterinary Association (2013), 178

Osteochondrosis (OC) is the most common developmental orthopaedic disease in horses and represents a major problem to the horse industry. The complete mechanism of this multifactorial disease is not yet ... [more ▼]

Osteochondrosis (OC) is the most common developmental orthopaedic disease in horses and represents a major problem to the horse industry. The complete mechanism of this multifactorial disease is not yet elucidated, but it is accepted that OC lesions are the result of intrinsic genetic and external factors. The aim of the present work was to evaluate the relationship between breeding management and OC. Breeding conditions were recorded, and a radiological examination was performed in 223 foals. Feeding practice and housing management were analysed in a multivariate model to determine risk factors for OC in three periods: gestation, birth to weaning and weaning to one-year-old. The major breakthrough of this study is the significant relationship between OC development and (1) the maternal nutrition during gestation and (2) the type of housing of the foals during their first year. It appears that mares fed with concentrates during gestation are more likely to produce foals that are subsequently affected by OC compared with other mares (P<0.05). Foals housed exclusively at pasture until one year of age are significantly less affected than foals exclusively housed in box or, alternatively, in box and at pasture (P<0.05). These results underline the role of the energy metabolism and the level of exercise in the aetiologic process of the disease, and help to develop preventive strategies during the crucial period of gestation to one year of age of the foal. [less ▲]

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See detailSevoflurane inhibits equine myeloperoxidase release and activity in vitro.
MINGUET, Grégory ULg; de la Rebière de Pouyade, Geoffroy ULg; Franck, Thierry ULg et al

in Veterinary Anaesthesia & Analgesia (2013), 40

Objective To investigate the effects of the volatile anaesthetic sevoflurane on the release of total and active myeloperoxidase (MPO) by non-stimulated and stimulated polymorphonuclear neutrophils (PMNs ... [more ▼]

Objective To investigate the effects of the volatile anaesthetic sevoflurane on the release of total and active myeloperoxidase (MPO) by non-stimulated and stimulated polymorphonuclear neutrophils (PMNs) in whole blood from healthy horses. Study design In vitro experimental study. Animals Adult healthy horses. Methods Samples of whole venous blood were collected and incubated in air or in air plus 2.3% or 4.6% sevoflurane for 1 hour. PMNs were stimulated with N-formyl-methionyl-leucyl-phenylalanine (fMLP), with a combination of cytochalasin B (CB) and fMLP or with phorbol myristate acetate (PMA). Total and active MPO contents released by PMNs in blood were measured by enzyme-linked immunosorbent assay (ELISA) and specific immunological extraction followed by enzymatic detection (SIEFED) respectively. Additional experiments were performed to assess the effect of sevoflurane on the peroxidase and chlorination cycles of purified equine MPO using Amplex Red and 3'-(p-aminophenyl) fluorescein as fluorogenic substrates respectively. Results As compared with air alone, 1 hour exposure of whole blood to 4.6% sevoflurane in air significantly inhibited the release of total and active MPO by unstimulated and both fMLP- and CB + fMLP-stimulated PMNs but not by PMA-stimulated PMNs. Although 2.3% sevoflurane had no effect on total MPO release by unstimulated and stimulated PMNs, it significantly reduced the release of active MPO by unstimulated and fMLP-stimulated PMNs. Additionally, sevoflurane reversibly inhibited the activity of MPO, especially the peroxidase cycle of the enzyme. Conclusions and clinical relevance Although our experimental study was not designed to assess the effects of sevoflurane in vivo, this inhibition of MPO release and activity may have relevance for anaesthetized horses and deserves further studies to examine the clinical importance of these findings. [less ▲]

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See detailAltered mitochondrial oxidative phosphorylation capacity in horses suffering from polysaccharide storage myopathy
Tosi, Irène ULg; Art, Tatiana ULg; Cassart, Dominique ULg et al

Poster (2013)

Introduction: Exertional rhabdomyolyses are a common cause of exercise intolerance in the equine athlete, and Polysaccharide Storage Myopathy (PSSM) is a widely described muscular pathology. It is ... [more ▼]

Introduction: Exertional rhabdomyolyses are a common cause of exercise intolerance in the equine athlete, and Polysaccharide Storage Myopathy (PSSM) is a widely described muscular pathology. It is characterized by an accumulation of abnormal glycogen in myofibers due to a genetic defect in the skeletal muscle glycogen synthase (GYS1) enzyme. We hypothesized that the energetic production through the oxidative phosphorylation (OXPHOS) in muscular mitochondria might be impaired in type-1 PSSM-affected horses. Material and Methods: Eight horses with a history of exertional rhabdomyolysis were tested for the GYS1 mutation. Muscle biopsies were collected and used for histological analysis and high resolution respirometry (HRR). HRR values from 3 groups of horses (PSSM-positive horses, horses with a history of myopathy but negative to PSSM and healthy controls) were compared using a linear mixed model to take into account repeated (2-3 times) measurements made for each horse. Results: In four horses histology revealed an accumulation of abnormal glycogen in myofibers. A severe depression of the maximal OXPHOS capacity was observed by HRR in all horses with exertional rhabodmyolysis, with lower values in PSSM-positive cases. Conclusions: Our study shows a severe decreased OXPHOS capacity in PSSM-affected horses. PSSM is considered primarily a defect in glycogen synthesis but altered OXPHOS might play a central role in its pathogenesis. [less ▲]

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See detailTriphenylphosphonium salts of 1,2,4-benzothiadiazine 1,1-dioxides related to diazoxide targeting mitochondrial ATP-sensitive potassium channels
Constant-Urban, C.; Charif, M.; Goffin, Eric ULg et al

in Bioorganic & Medicinal Chemistry Letters (2013), 23

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See detailDifferentiation between stoichiometric and anticatalytic antioxidant properties of benzoic acid analogues: A structure/redox potential relationship study.
Franck, Thierry ULg; Mouithys-Mickalad, Ange ULg; Robert, Thierry ULg et al

in Chemico-biological interactions (2013), 206(2), 194-203

We investigated the antioxidant activities of some phenolic acid derivatives on a cell free system and on cellular and enzymatic models involved in inflammation. The stoichiometric antioxidant activities ... [more ▼]

We investigated the antioxidant activities of some phenolic acid derivatives on a cell free system and on cellular and enzymatic models involved in inflammation. The stoichiometric antioxidant activities of phenolic acid derivatives were studied by measuring their capacity to scavenge the radical cation 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS+) and reactive oxygen species (ROS) produced by stimulated neutrophils. The anticatalytic antioxidant capacity of the molecules was evaluated on the activity of myeloperoxidase (MPO), an oxidant enzyme present in and released by the primary granules of neutrophils. The ROS produced by PMA-stimulated neutrophils were measured by lucigenin-enhanced chemiluminescence (CL) and the potential interaction of the molecules with MPO was investigated without interferences due to medium by Specific Immuno-Extraction Followed by Enzyme Detection (SIEFED). The antioxidant activities of the phenolic compounds were correlated to their redox potentials measured by differential pulse voltammetry (DPV), and discussed in relation to their molecular structure. The ability of the phenolic molecules to scavenge ABTS radicals and ROS derived from neutrophils was inversely correlated to their increased redox potential. The number of hydroxyl groups (three) and their position (catechol) were essential for their efficacy as stoichiometric antioxidants or scavengers. On MPO activity, the inhibitory capacity of the molecules was not really correlated with their redox potential. Likewise, for the inhibition of MPO activity the number of OH groups and mainly the elongation of the carboxylic group were essential, probably by facilitating the interaction with the active site or the structure of the enzyme. The redox potential measurement, combined with ABTS and CL techniques, seems to be a good technique to select stoichiometric antioxidants but not anticatalytic ones, as seen for MPO, what rather involves a direct interaction with the enzyme. [less ▲]

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See detailEffects of diazoxide, benzothiadiazine and benzopyrane derivatives on mitochondrial proton and electron leaks of cardiomyocytes (H9C2 cell line).
Mouithys-Mickalad, Ange ULg; Ceusters, Justine ULg; Charef, M et al

Poster (2013)

Background: Mitochondria are double membrane- organelles that play a central role in cellular metabolism, calcium homeostasis and redox signaling. They have been also considered as main producers of ... [more ▼]

Background: Mitochondria are double membrane- organelles that play a central role in cellular metabolism, calcium homeostasis and redox signaling. They have been also considered as main producers of adenosine triphosphate (ATP) and reactive oxygen species (ROS). In many cancer cells those organelles become dysfunctional leading to a shift of energy metabolism from oxidative phosphorylation to active glycolysis and an increase of ROS generation. According to Warberg’ theory, cancer damage might occur at the mitochondrial level, affecting tiny structures within each cell implicated in the energy production through ATP. New insight is that mitochondria might be a good therapeutic target for metabolic syndromes, ischemia/reperfusion injury and organs transplantation. Therefore, search for novel molecules able to keep mitochondria functional are of relevant interest. Methodology: Cardiomyocytes (H9C2 cells) were from ATCC (USA) and grown till confluence. The basal cellular respiratory rate, proton and electron leaks as well as ATP production were measured with the High Resolution Oxygraphy (Oroboros, Austria). All compounds: diazoxide (DIAZ), diazoxide –related analogs (1: BPDZ-259, 2: BPDZ-444), and benzopyran derivatives (3: BPDZ-490, 4: BPDZ-711) were tested at final concentration of 10-5 M, except when specified and compared to control samples (cells with or without DMSO). Results and conclusion: The basal respiratory rate of H9C2 cells (5x106/mL) was changed depending on the chemical structure of the tested compounds: e.g. compound 3 strongly enhanced the routine respiration, while 4 displayed a marked lowering effect. In contrast, the addition of similar concentration of benzothiadiazin derivatives (1, 2) had no effect on routine respiration but also on the other respiratory parameters such as oligomycin-induced leak and ATP production. Similar profile was obtained with the reference molecule: diazoxide. Overall, our findings indicate that both diazoxide-like analogues (1 and 2) and diazoxide were without significant effect on basal respiration, ATP production, even on maximal respiration. Interestingly, two derivatives show opposite effects: compound 3 behaves as a uncoupling agent and the other one (4) exhibits a real lowering effect on respiration but that was reversible. The latter effect might be of interest if this kind of molecules could be used for further use as an agent for organ conservation during transplantation. Our results also demonstrate that diazoxide, a well-known Mito-KATP opener, did not exert its effect beside of clinical situation like ischemia/reperfusion injury. [less ▲]

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See detailAn in vitro whole blood model to test the effects of different stimuli conditions on the release of myeloperoxidase and elastase by equine neutrophils.
Ceusters, Justine ULg; Serteyn, Didier ULg; MINGUET, Grégory ULg et al

in Veterinary Immunology and Immunopathology (2012), 150(3-4), 221-7

Horses are particularly sensitive and exposed to excessive inflammatory responses evolving toward an important stimulation of polymorphonuclear neutrophils (PMNs). The aim of this work was to stimulate ... [more ▼]

Horses are particularly sensitive and exposed to excessive inflammatory responses evolving toward an important stimulation of polymorphonuclear neutrophils (PMNs). The aim of this work was to stimulate equine neutrophils in whole blood and to evaluate their response by measuring the release of total and active myeloperoxidase (MPO) and total elastase, considered as markers of neutrophil stimulation and degranulation. Because of the critical importance of the concomitant presence of LPS and TNF-alpha in equine pathological situations, we combined these two natural mediators to stimulate PMN and compared the response with those obtained after the PMN stimulation with each mediator used alone and well-known artificial stimulation systems such as 12-phorbol 13-myristate acetate (PMA) and the combination of cytochalasin B (CB) and N-formyl-methionyl-leucyl-phenylalanine (fMLP). All the activation systems, PMA, CB/fMLP, TNF-alpha, LPS and LPS/TNF-alpha, induced a significant release of total MPO in whole blood but only the combinations CB/fMLP and LPS/TNF-alpha significantly favored the release of active MPO. Regarding the total elastase, we did not observe a significant release in all the stimulated conditions except with PMA. It appears clearly that the choice of the neutrophil stimulation model is fundamental for the selection of potentially active pharmacological agents, especially on MPO activity. [less ▲]

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See detailINFLUENCE OF MYELOPEROXIDASE ACTIVITY ON EQUINE POST-THAW SEMEN QUALITY
Ponthier, Jérôme ULg; Franck, Thierry ULg; Parrilla Hernandez, Sonia ULg et al

in Edeas, Marvin (Ed.) Proceedings of the 2nd ISANH World congress on Fertility and Antioxidants (2012, December 06)

This study confirms that active MPO is associated with cellular fraction of the ejaculate, as previously suggested for total MPO concentration in thawed semen (2). However, active MPO concentrations were ... [more ▼]

This study confirms that active MPO is associated with cellular fraction of the ejaculate, as previously suggested for total MPO concentration in thawed semen (2). However, active MPO concentrations were dramatically lower than total MPO concentrations observed in equine semen (3), which could be explained by presence of inactive MPO subunits in semen. MPO activity in sperm-rich pellet can be used as a predictive marker of post-thaw semen quality. Moreover, methods to inhibit MPO should be investigated in semen. [less ▲]

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See detailClinical Assessment of alfaxolone in rabbits
Tutunaru, Alexandru-Cosmin ULg; Sonea, Alexandru; Drion, Pierre ULg et al

Poster (2012, October 19)

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See detailSurgical treatment of open joint injuries: a retrospective study of 22 horses.
Salciccia, Alexandra ULg; de la Rebière de Pouyade, Geoffroy ULg; Evrard, Laurence ULg et al

Conference (2012, October 19)

Aims: To evaluate the prognosis of horses treated surgically for open joint injuries. Methods: The medical records of horses admitted for surgical treatment of a wound in communication with a joint were ... [more ▼]

Aims: To evaluate the prognosis of horses treated surgically for open joint injuries. Methods: The medical records of horses admitted for surgical treatment of a wound in communication with a joint were reviewed. A telephone questionnaire was used for the long term evaluation. Fisher’s exact tests were used for the statistical analyses. Results: Twenty two horses were included in the study with the following distribution of lesions: 6 carpi, 6 fetlocks, 4 tibiotarsal joints, 2 proximal and 3 distal interphalangeal joints, 1 elbow. The duration of the injury before referral ranged from 3 hours to 10 days. Surgical treatment consisted of 1-3 joint lavages. Of the 22 horses, 4 were euthanatized during hospitalization and 18 were discharged. After discharge, 3 horses died due to colic, 2 were lost and 13 were still alive. The survival was not influenced by the duration of the wound. All horses with cutaneous defects less than 5 cm and all horses affected in the lower limb (below the level of the canon) were discharged. Having an affected joint proximal to the canon was significantly associated to the need of multiple surgeries (OR: 17.5; p= 0.024). Conclusions: Even if the prognosis remains guarded for open joint injuries, a long delay between injury and treatment should not be systematically associated with a bad prognosis for survival. Open joint injuries of the lower limb were associated with survival. They required less often multiple articular lavages than open joint injuries of the upper limb, warranting thus a better prognosis. [less ▲]

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See detailClinical aspects of alfaxalone use in rabbits. Partial results.
Tutunaru, Alexandru-Cosmin ULg; Sonea, P; Drion, Pierre ULg et al

in BCLAS, ESLAV (Ed.) Proceedings BCLAS, ESLAV & ECLAM Scientific Meeting (2012, October 15)

Alfaxalone, a synthetic neuroactive steroid, has been shown to induce smooth general anesthesia in wild and companion rabbits. The aim of the study was to test the effect of alfaxolone in experimental ... [more ▼]

Alfaxalone, a synthetic neuroactive steroid, has been shown to induce smooth general anesthesia in wild and companion rabbits. The aim of the study was to test the effect of alfaxolone in experimental rabbits without concomitant oxygen supplementation.Respiratory status was assessed by measuring the duration of the post-induction apnea, the respiratory rate, the oxygen saturation of hemoglobin in the peripheral blood (SpO2) and the end-tidal CO2 (EtCO2) Cardiac function was assessed by monitoring heart rate using a pulse oximeter. The degree of anesthesia was monitored by evaluating ear and paw pinch reflex, as well as ocular signs such as nystagmus, exophtalmia and the loss of palpebral and corneal reflexes. [less ▲]

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See detailLes kératomes: lésions et traitements chez 19 chevaux
Salciccia, Alexandra ULg; de la Rebière de Pouyade, Geoffroy ULg; Bolen, Géraldine ULg et al

Conference (2012, October 12)

Introduction: Les kératomes, masses hyperplasiques de kératine, croissent entre la phalange distale (P3) et la paroi du sabot et provoquent des boiteries chez le cheval. La littérature comporte de ... [more ▼]

Introduction: Les kératomes, masses hyperplasiques de kératine, croissent entre la phalange distale (P3) et la paroi du sabot et provoquent des boiteries chez le cheval. La littérature comporte de nombreux rapports de cas isolés mais peu de séries cliniques. Matériel et méthodes: Les dossiers cliniques des cas de kératomes présentés depuis 2004 à la faculté de médecine vétérinaire de Liège ont été revus. Résultats: Vingt-trois kératomes ont été diagnostiqués sur 19 chevaux boiteux. Un cheval présentait 2 kératomes sur le même pied et 3 chevaux présentaient 2 kératomes sur des pieds différents. Sur les 22 pieds atteints, les lésions observées étaient: un abcès, souvent récidivant (18 cas), une déformation de la muraille (6 cas), une déviation de la ligne blanche (6 cas) et une seime (3 cas). Un kératome était situé sous la sole et 22 sous la muraille. La durée des symptômes variait de 2 semaines à 15 ans (plus d'un an sur 12 pieds). Sur les radiographies, une lyse par compression de P3 était visible en regard de 20 kératomes. Le cheval présentant 2 kératomes sur le même pied a été euthanasié. Trois kératomes ont été traités de manière conservative. Dix-huit kératomes (chez 16 chevaux) ont été excisés chirurgicalement dont 16 par avulsion complète de muraille, 1 par avulsion partielle de muraille et 1 par curetage d'une portion de sole. Aucune complication postopératoire n'a été observée dans 7/18 cas (39%). Les complications rencontrées étaient: une granulation excessive (10 cas), une douleur importante pendant plusieurs jours (5 cas), une légère infection (4 cas), un enfoncement de P3 en regard de la zone avulsée (2 cas) et une récidive (2 cas, dont 1 a été réopéré avec succès ultérieurement). Un cheval est encore en convalescence au moment de cette étude. Le suivi à long terme d’un cheval a été perdu. Des 14 chevaux repris dans l'évaluation postopératoire, 1 a été euthanasié pour une autre raison que le kératome, 3 chevaux ont gardé une boiterie résiduelle au trot et 10 chevaux ont récupéré leur niveau d'activité. Discussion: La littérature décrit les kératomes comme rares1. Or, 21% des chevaux de cette étude (4 chevaux sur 19) présentaient plusieurs kératomes. De plus, dans 54 % des cas, les symptômes duraient depuis plus d’un an. Dès lors, il apparait que les kératomes sont sous diagnostiqués ou le sont souvent tardivement. La chronicité et l’étendue des kératomes de cette étude n’a que très rarement permis leur résection par avulsion partielle de muraille, technique de choix dont les complications postopératoires sont moindres². Un diagnostic précoce par inspection minutieuse des sabots, particulièrement en cas d’abcès de pied récidivant permettrait une excision par une technique moins invasive, qui diminuerait la convalescence et les complications postopératoires. Bibliographie: 1. Sundberg, J.P. et al. Neoplasms of Equidae. Journal of American veterinary medical association, 1977, 170: 150-152. 2. Boys Smith SJ. et al. Complete and partial hoof wall resection for keratoma removal: postoperative complications and final outcome in 26 horses (1994-2004). Equine Veterinary Journal, 2006, 38 (2): 127-133. [less ▲]

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See detailIntérêt d’un système de traitement des plaies par pression négative chez le cheval : Etat des lieux et cas cliniques
de la Rebière de Pouyade, Geoffroy ULg; Salciccia, Alexandra ULg; Gougnard, Alexandra ULg et al

Conference (2012, October 12)

Les plaies traumatiques ou chirurgicales nécessitent souvent une cicatrisation par seconde intention lorsque la fermeture primaire n’est pas possible ou échoue. La fermeture assistée par le vide (Vacuum ... [more ▼]

Les plaies traumatiques ou chirurgicales nécessitent souvent une cicatrisation par seconde intention lorsque la fermeture primaire n’est pas possible ou échoue. La fermeture assistée par le vide (Vacuum Assisted Closure : VAC) est une méthode active et non invasive qui expose le lit de la plaie à une pression sub-atmosphérique locale afin de promouvoir sa cicatrisation. Elle stimule la granulation et la perfusion sanguine locale et réduit significativement l’œdème et la charge bactérienne.1 Cette technique s’est révélée efficace dans le traitement des plaies aiguës et chroniques chez l’homme. Chez le cheval, la méthode est encore peu décrite.2,3 Nous rapportons ici l’application et les effets de la thérapie des plaies par pression négative à travers les cas traités à la clinique vétérinaire universitaire de Liège depuis 2010. Les plaies étaient chirurgicalement débridées avant l’application du système VAC. Une mousse en polyuréthane était appliquée après avoir été adaptée à la géométrie de la plaie. Un champ plastique adhésif était alors apposé sur la plaie pour créer un environnement hermétique et un orifice d’1 cm2 était créé au centre du système pour appliquer la ventouse du tuyau d’aspiration. Cette ligne était alors raccordée à l’appareil d’aspiration contrôlée pour maintenir une pression continue de -125 mmHg dans la plaie. Quinze cas ont été recensés: 3 plaies intra-articulaires (au carpe, au coude et au grasset), 3 sur la face dorsale du canon postérieur, 2 sur la face dorsale du jarret, 1 sur la pointe du calcanéum, 2 sur l’encolure et 4 plaies de laparotomie infectées. Le traitement a accéléré la croissance du tissu de granulation, stimulé la contraction des plaies et favorisé la cicatrisation dans la majorité des cas (12/15). Les plaies apparaissaient aussi plus saines et présentaient moins de sécrétions lors des changements de bandages. Des résultats plus modérés ont été notés chez les 3 autres cas. Quelques complications mineures comme des irritations cutanées ont été observées. Le traitement de plaies contaminées nous a orientés vers l’usage d’une mousse de polyuréthane argenté mais d’autres types de matrice sont disponibles et permettent de modifier le comportement de la plaie. L’usage d’une mousse de polyvinyl Alcool s’est ainsi avéré efficace pour améliorer l’adhésion des greffons lors d’une greffe de peau chez le cheval.3 La principale limite du traitement réside dans la difficulté technique de maintenir la plaie sous vide lors d’atteinte de régions très mobiles comme les articulations. En conclusion, le système VAC semble très prometteur pour optimiser la qualité de la cicatrisation et réduire la durée de l’hospitalisation. 1 - Morykwas, M.J., Argenta, L.C., et al. Vacuum-assisted closure: a new method for wound control and treatment: animal studies and basic foundation. Ann. Plast. Surg. 1997, 38, 553-562 2 - Gemeinhardt K.D., Molnar J.A. Vacuum-assisted closure for management of a traumatic neck wound in a horse. Equine Vet. Educ. 2005, 17, 27-33 3 - Jordana M., Pint E., et al. The use of vacuum-assisted wound closure to enhance skin graft acceptance in a horse. Vlaams Diergeneeskundig Tijdschrift 2011, 80, 343-350 [less ▲]

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See detailSimultaneous measurement of protein-bound 3-chlorotyrosine and homocitrulline by LC-MS/MS after hydrolysis assisted by microwave: application to the study of myeloperoxidase activity during hemodialysis.
Delporte, Cedric; Franck, Thierry ULg; Noyon, Caroline et al

in Talanta (2012), 99

A high degree of uremia is common in patients with end-stage renal disease and has been linked to the development of chronic inflammation and cardiovascular diseases. In conditions where transplantation ... [more ▼]

A high degree of uremia is common in patients with end-stage renal disease and has been linked to the development of chronic inflammation and cardiovascular diseases. In conditions where transplantation is not possible, uremia can be reduced by hemodialysis although the repeated interventions have been implicated in loss of renal function, partially as a result of chronic inflammation and/or oxidative stress processes. In this context, it has been suggested that myeloperoxidase (MPO) can contribute to the oxidative stress during hemodialysis and to the cardiovascular risk. Protein damages due to MPO activity have never been assessed during hemodialysis although two of its reaction products, 3-chlorotyrosine and homocitrulline, are of interest. Indeed, the first one is a specific product of MPO activity and the formation of the second one could be catalyzed by MPO. In order to analyze these products in plasma proteins, a total hydrolysis method followed by liquid chromatography mass spectrometry analysis was developed. Different conditions of hydrolysis were tested and the optimized procedure was assessed for complete hydrolysis and artifactual chlorination. Finally, the method was used for analyzing 3-chlorotyrosine and homocitrulline in plasma proteins during a hemodialysis session in fifteen patients and data were related to measurements of MPO concentration and activity. Both increases in MPO activity and protein-bound 3-chlorotyrosine were observed, highlighting the involvement of MPO in oxidative stress during hemodialysis and further demonstrating the link between hemodialysis and cardiovascular diseases. [less ▲]

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