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See detailEvaluation of sedation depth and quality of performed procedures following administration of α2-agonists combined or not with butorphanol in horses.
Barbry, Stephanie; Verwilghen, Denis ULg; Van Galen, Gaby ULg et al

Poster (2008, October)

Several molecules available in equine medicine provide profound sedation and good analgesia when performing diagnostic or surgical procedures. They differ slightly in degree of action, as well in time as ... [more ▼]

Several molecules available in equine medicine provide profound sedation and good analgesia when performing diagnostic or surgical procedures. They differ slightly in degree of action, as well in time as in depth. The aim of this study was to evaluate the depth of sedation and quality of performing a standardized procedure after injection of an 2-agonist alone or associated with butorphanol. Horses presented to the Equine Clinic of Liege University from December 2007 to June 2008 for an endoscopic, dental, radiological or ophthalmological examination of which owner and clinician agreed to include the horse in the study were retained. All horses (n=133) were sedated in a randomized double blind fashion using one of the six following protocols: detomidine (D) (0.01mg/kg IV), romifidine (R) (0.04mg/kg IV), or xylazine (X) (0.5mg/kg IV) alone or associated with butorphanol (B) (0.02mg/kg IV). Stability, head position and the reaction to a stimulus (auditive and visual) were evaluated before the start of the procedure. An overall subjective grade using a 10 cm line scale was given to evaluate sedation depth (ranging from no effect to maximal depth on line) and the quality of the performed procedure (ranging from not realised to ideal examination). For each specific procedure, the same clinician experienced in his particular field evaluated the parameters. Data were related by a variance analysis using SAS system and considered significant if P<0.01. The used protocols significantly influenced both sedation depth and examination quality. Sedation depth increased significantly when B was added in all the three 2-agonists used (mean ± SD): D: 6.46±0.33; D+B: 7.06±0.37; R: 4.93±0.46; R+B: 7.04±0.45; X: 4.94±0.43; X+B: 7.75±0.40. Detomidine provided a significantly better quality of the examination compared to romifidine and xylazine. The addiction of butorphanol significantly decreased the examination quality when added to detomidine and increased the quality when combined with romifidine and xylazine. Mean (± SD) of the quality of performed procedures: D: 8.41±0.54; D+B: 6.06±0.64; R: 6.68±0.71; R+B: 7.16±0.67; X: 4.47±0.67; X+B: 4.52±0.64. Comparison of sedation depth and quality of performed examination revealed an increase in quality of examination scores with increasing depth of sedation until a certain level. As from sedation depths with scores of 8 or more, a decrease in quality of examination was encountered. This report shows the important effect of butorphanol addition on sedation. However, increasing sedation depth was not necessarily linked to a better quality of procedure, notably due to difficulties in handling strongly sedated horses for the procedures performed in this study. [less ▲]

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See detailEquine frozen semen parameters in relation with total myeloperoxidase concentration
Ponthier, Jérôme ULg; Franck, Thierry ULg; Mottart, Evelyne et al

in Animal Reproduction Science (2008, September 15)

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See detailMandibular osteodistraction for correction of deep bite class II malocclusion in a horse
Verwilghen, Denis ULg; Van Galen, Gaby ULg; Vander Heyden, Laurent ULg et al

in Veterinary Surgery : The Official Journal of the American College of Veterinary Surgeons (2008), 37(6), 571-579

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See detailDiselenide Derivative, Potential Successor of Ebselen with High Antioxidant Activity: Assessment on in vitro Models
Mareque-Faez, Juan; Deby, Carol; Lamy, Maurice ULg et al

in Resource-Full Chemistry (2008, May 24)

Oxidative stress plays a key role in several pathophysiological events, including the attack of DNA, cell membrane damage and signaling pathways disruption. The harmful effect of oxidant stress has been ... [more ▼]

Oxidative stress plays a key role in several pathophysiological events, including the attack of DNA, cell membrane damage and signaling pathways disruption. The harmful effect of oxidant stress has been attributed to a high production of reactive oxygen species (ROS) followed by a depletion of antioxidant enzymes, glutathione peroxidase (GPx), a mammalian selenoenzyme which functions as a catalytic antioxidant, has been described to protect various organisms against oxidative stress. In the past two decades, the design of small weight molecules such as ebselen (PZ 51, 2-phenyl-1,2-benzoisoselenazol-3(2H)-one), has renewed the interest of synthetic analogues able to mimic the GPx activity. From four in vitro in vitro models, we previously showed that ebselen and some of its analogues (compounds 1, 2, 3 and 4) not only behaved at various degrees as GPx-like mimics but also as antioxidants especially for diselenide derivative (3). The present study deals with the antioxidant effect of diselenide derivative (3) versus ebselen on cellular model and the enzyme myeloperoxidase (MPO) activity using the in vitro systems. Derivative (3) has been chosen because of its interesting antioxidant profile in cell-free systems. [less ▲]

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See detailAtypical myopathy (myoglobinuria): 5 cases-based questions.
Votion, Dominique ULg; Serteyn, Didier ULg

Scientific conference (2008, April)

Case-based questions ... and answers!

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See detailMyeloperoxidase Assay in Plasma and Peritoneal Fluid of Horses with Gastrointestinal Disease
Grulke, Sigrid ULg; Franck, Thierry ULg; Gangl, Monika et al

in Canadian Journal of Veterinary Research = Revue Canadienne de Recherche Vétérinaire (2008), 72(1), 37-42

Gastrointestinal disorders, especially strangulating intestinal obstructions, are still a major cause of illness and death in the horse. Circulating lipopolysaccharides may activate both neutrophils and ... [more ▼]

Gastrointestinal disorders, especially strangulating intestinal obstructions, are still a major cause of illness and death in the horse. Circulating lipopolysaccharides may activate both neutrophils and monocytes. The activated neutrophils release myeloperoxidase (MPO), a specific enzyme with strong oxidative activity. The aim of this study was to evaluate MPO concentrations in the plasma and peritoneal fluid (PF) of horses with colic and to check the hypothesis that these concentrations would be higher in a case of strangulating obstruction than in cases of nonstrangulating disease. By using a specific enzyme-linked immunosorbent assay for equine MPO, we determined the MPO concentrations in horses admitted to a clinic for colic. Horses with nonstrangulating or strangulating obstruction of the large intestine (NSLI or SLI), strangulating obstruction of the small intestine (SSI), or inflammatory bowel disease (IBD) were compared with healthy horses. The horses with SLI, SSI, or IBD had significantly higher MPO levels in plasma and PF than did those in the other 2 groups. The mean plasma level was significantly higher in the horses with NSLI than in the healthy horses. High MPO values in PF indicated necrotic bowel. These results show that neutrophil activation occurs during nonstrangulating and strangulating intestinal obstruction in horses and that the plasma and PF MPO concentrations may be a marker of the severity of the disease. [less ▲]

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See detailApproche diagnostique des myopathies chez le cheval
Van Galen, Gaby ULg; Serteyn, Didier ULg; Amory, Hélène ULg et al

in Le nouveau Praticien vétérinaire équine (2008), 15

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See detailInhibitory effect of curcuminoids and tetrahydrocurcuminoids on equine activated neutrophils and myeloperoxidase activity
Franck, Thierry ULg; Kohnen, Stephan ULg; Grulke, Sigrid ULg et al

in Physiological Research (2008), 57(4), 577-587

In the horse, the inflammation response to various pathologies (intestinal strangulations, laminitis, etc.) involves an excessive stimulation of the polymorphonuclear neutrophils releasing reactive oxygen ... [more ▼]

In the horse, the inflammation response to various pathologies (intestinal strangulations, laminitis, etc.) involves an excessive stimulation of the polymorphonuclear neutrophils releasing reactive oxygen species (ROS) and myeloperoxidase (MPO). The aim of the present work was to study the effect of natural polyphenols, curcuminoids and tetrahydrocurcuminoids (THC) on isolated stimulated equine neutrophils and on the activity of purified MPO. The ROS production and the release of MPO by activated neutrophils were measured by chemiluminescence and ELISA techniques, respectively. The activity of purified MPO was measured by studying its nitration, chlorination or oxidation capacity and by using an original method called SIEFED allowing the study of drug interaction with the enzyme without interferences of the medium. Curcuminoids and THC had dose-dependent inhibitory effects on ROS production and MPO release by activated neutrophils and on purified MPO activity. We suggest that the higher efficacy of curcuminoids versus THC could be explained, at least partially, by its chemical structure: the conjugated double bounds and the plane structure of curcuminoids made easier the neutralization of the radical species generated by activated neutrophils and the interaction of the drug with the active site of MPO. These inhibitory effects of curcuminoids on the oxidant activity of equine neutrophils and on MPO activity open therapeutic perspectives in equine pathologies with excessive inflammatory reactions. [less ▲]

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See detailThe Effect of Colic on Oxygen Extraction in Horses
Cambier, Carole ULg; Wierinckx, Maude ULg; Grulke, Sigrid ULg et al

in Veterinary Journal (2008), 175(1), 102-107

Blood oxygen transport and oxygen extraction were assessed in horses with colic. A gravity score (GS) ranging from 1 to 3 was attributed to each colic case with healthy horses used as controls. Jugular ... [more ▼]

Blood oxygen transport and oxygen extraction were assessed in horses with colic. A gravity score (GS) ranging from 1 to 3 was attributed to each colic case with healthy horses used as controls. Jugular venous and carotid arterial blood samples were collected and concentrations of 2,3-diphosphoglycerate, adenosine triphosphate, inorganic phosphate and chloride were determined. pH and partial pressures of carbon dioxide (PCO(2)), and oxygen (PO(2)) were also measured. Oxygen equilibrium curves (OEC) were constructed under standard conditions and oxygen extraction ratios calculated. Haemoglobin oxygen affinity measured under standard conditions (P50(std)) was unchanged in colic horses compared with healthy controls. Horses with the highest GS, i.e. 3 had lower blood pH values than healthy animals. Arterial and venous partial pressures of oxygen at 50% haemoglobin saturation (P50(a) and P50(v)) were significantly higher in horses suffering from colic (GS=3) than in healthy horses. The oxygen extraction ratio was also significantly increased in colic horses with a GS of 3. A rise in the oxygen extraction ratio detected in the most severely affected animals seemed to reflect the compensatory properties of the oxygen transport system where extraction of oxygen from the blood increases when systemic oxygen delivery decreases, as might be anticipated in horses with colic. [less ▲]

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See detailEffect of acepromazine on whole blood viscosity in horses
Sandersen, Charlotte ULg; Serteyn, Didier ULg; Dringenberg, Bernhard et al

in Proceedings (2008)

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See detailEquine atypical myopathy: a review
Votion, Dominique ULg; Serteyn, Didier ULg

in Veterinary Journal (2008), (178), 185-190

Atypical myopathy (AM) is an acute rhabdomyolysis syndrome that occurs at irregular intervals in grazing equines. An increasing number of outbreaks have been reported in recent years, including some from ... [more ▼]

Atypical myopathy (AM) is an acute rhabdomyolysis syndrome that occurs at irregular intervals in grazing equines. An increasing number of outbreaks have been reported in recent years, including some from countries where the disease has not previously been diagnosed. In this review, clinical and other details of outbreaks of AM are analysed to better define its epidemiological profile. Potential aetiologies are discussed, the short clinical course of AM is described and the main biochemical and pathological findings are considered. Recommendations for medical management are suggested, based on a review of clinical reports. Biochemical and histopathological findings have been integrated in order to characterise the physiopathology of AM. There is an ongoing requirement to record new cases of this syndrome, ideally through an epidemiological network. [less ▲]

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See detailAtypical myopathy: new insights into the pathophysiology, prevention and management of the condition
Van Galen, Gaby ULg; Serteyn, Didier ULg; Amory, Hélène ULg et al

in Equine Veterinary Education (2008), 20(5), 234-238

Detailed reference viewed: 39 (7 ULg)