References of "Serteyn, Didier"
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See detailA comparison between pulse contour analysis and Doppler echocardiographic measurements of cardiac output in awake horses
Guidi, E; Amory, Hélène ULg; Borde, Laura ULg et al

in In Proceedings of the 3rd Annual Congress of the European College of Equine Internal Medicine (ECEIM) (2009)

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See detailA new easy method for specific measurement of active myeloperoxidase in human biological fluids and tissue extracts
Franck, Thierry ULg; Kohnen, Stephan; Zouaoui Boudjeltia, Karim et al

in Talanta (2009), 80

The SIEFED (“Specific Immunological Extraction Followed by Enzymatic Detection”) method already developed for the specific detection of the activity of equine myeloperoxidase (MPO) was adapted for the ... [more ▼]

The SIEFED (“Specific Immunological Extraction Followed by Enzymatic Detection”) method already developed for the specific detection of the activity of equine myeloperoxidase (MPO) was adapted for the specific measurement of active human MPO in biological fluids or tissue extracts. [less ▲]

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See detailDie exokrine Pankreasfunktion beim kranken Pferd
Sandersen, Charlotte ULg; Serteyn, Didier ULg; Grulke, Sigrid ULg

in Vervuert; Achenbach; Gäbel (Eds.) et al LBH, Proccedings 5. Leipziger Tieraerzte Tag (2009)

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See detailEquine neutrophil elastase in plasma, laminar tissue, and skin of horses administered black walnut heartwood extract.
de la Rebière de Pouyade, Geoffroy ULg; Riggs, L. M.; Moore, J. N. et al

in Veterinary immunology and immunopathology (2009)

Laminitis is a local manifestation of a systemic inflammatory response that is characterized by neutrophil activation and movement of neutrophils into the laminar tissues. Given the evidence for the ... [more ▼]

Laminitis is a local manifestation of a systemic inflammatory response that is characterized by neutrophil activation and movement of neutrophils into the laminar tissues. Given the evidence for the involvement of neutrophils in the development of laminitis, we measured concentrations of neutrophil elastase, a serine protease released from the azurophilic granules of neutrophils, in plasma, skin and laminar tissues obtained from control horses and horses given black walnut heartwood extract (BWHE) to induce laminitis. Healthy horses (5-15 years old) were randomly assigned to 4 groups: 3 experimental groups given BWHE via nasogastric tube, and a control group given an equal volume of water. The experimental groups consisted of horses euthanized 1.5h (n=5), 3h (n=6) or 12h (n=10) after BWHE administration. Control horses (n=7) were euthanized 12h after intragastric administration of water. Plasma samples were collected in all horses of the control and 12h BWHE groups at 0, 1, 2, 3, 4, 6, 8, 10, and 12h after treatment, and laminar tissue and skin from the middle region of the neck were harvested at the time of euthanasia in all 1.5 and 3h BWHE horses, in 6 of the 12h BWHE horses and in 5 of the control horses. Plasma and tissue concentrations of neutrophil elastase were determined using an equine specific ELISA, and statistical significance was set at p<0.05. Plasma concentrations of neutrophil elastase in the BWHE group were significantly higher at 6 and 8h compared to the control group and at 8 and 10h compared to time 0. Concentrations of neutrophil elastase in skin and laminar tissue were significantly higher in the 3 and 12h BWHE groups compared to the control group. Concentrations of neutrophil elastase were significantly higher in the skin than in the lamina in the 12h BWHE horses. The administration of BWHE thus results in significant increases in the concentration of neutrophil elastase in the circulation, skin and laminar tissue. These results confirm a role for neutrophils in the developmental phase of laminitis, and the systemic nature of the inflammatory process. Furthermore, neutrophil elastase may play a key role in the disintegration of the hoof basal membrane and be a target for the development of new treatments for laminitis. [less ▲]

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See detailGestion médicale des myopathies: les premiers gestes
Votion, Dominique ULg; Van Galen, Gaby ULg; Caudron, Isabelle et al

in Pratique Vétérinaire Equine (2009), 41(numero special), 119-126

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See detailActivation of equine neutrophils by phorbol myristate acetate or N-formyl-methionyl-leucyl-phenylalanine induces a different response in reactive oxygen species production and release of active myeloperoxidase.
Franck, Thierry ULg; Kohnen, Stéphane; de la Rebière de Pouyade, Geoffroy ULg et al

in Veterinary Immunology and Immunopathology (2009)

Neutrophil (PMN) contribution to the acute inflammatory processes may lead to an excessive generation of reactive oxygen metabolites species (ROS) and secretion of granule enzymes. We compared the effects ... [more ▼]

Neutrophil (PMN) contribution to the acute inflammatory processes may lead to an excessive generation of reactive oxygen metabolites species (ROS) and secretion of granule enzymes. We compared the effects of either phorbol myristate acetate (PMA) or N-formyl-methionyl-leucyl-phenylalanine (fMLP) in combination with a pre-treatment by cytochalasin B (CB) on the production of ROS and the release of total and active myeloperoxidase (MPO) by isolated equine PMNs. The ROS production was assessed by lucigenin dependent chemiluminescence (CL) and ethylene release by alpha-keto-gamma-methylthiobutyric acid (KMB) oxidation. In the supernatant of activated PMNs, total equine MPO was measured by ELISA and active MPO by the SIEFED (Specific Immunologic Extraction Followed by Enzymatic Detection) technique that allows for the study of the interaction of a compound directly with the enzyme. The stimulation of PMNs with CB-fMLP only modestly increased the release of MPO, but more than 70% of released MPO was active. PMA stimulation markedly increased the production of ROS and release of MPO, but more than 95% of released MPO was inactive. When PMNs were pre-incubated with superoxide dismutase (SOD) prior to PMA activation, the lucigenin enhanced CL, which is linked to the superoxide anion (O(2)(-)) production, was much more decreased than KMB oxidation, linked to the hydroxyl-like radical production. The addition of SOD prior to the activation of PMNs by PMA also limited the loss of the activity of released MPO. These results confirm the key role of O(2)(-) generation in the ROS cascade in PMN and reveal its critical role on MPO inactivation. [less ▲]

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See detailBiochemical markers and radiographic scores as an evaluation for the osteoarticular status of Warmblood stallions.
Verwilghen, Denis ULg; Busoni, Valeria ULg; Salciccia, Alexandra ULg et al

(2009)

Establishing the osteoarticular status of the horse is often performed by means of radiological screening of the animals. Widespread blood sampling could potentially be an alternative to this procedure ... [more ▼]

Establishing the osteoarticular status of the horse is often performed by means of radiological screening of the animals. Widespread blood sampling could potentially be an alternative to this procedure which is time consuming and sometimes technically difficult. The aim of this study was to investigate the relationship between the radiological status of the horses and the levels of biochemical markers (BM) of cartilage degradation and synovial inflammation. Materials and Methods: A specific radiological scoring (RS) system was developed and applied on 63 stallions presented for studbook admission. Additionally, groups of horses were established according to the occurrence of osteochondrosis (OC), degenerative joint disease (DJD) and distal interphalangeal joint (DIPJ) effusion. Insulin growth factor-I, myeloperoxidases, Coll2-1 and Coll2-1NO2 were used as BM. The effects of age and weight on the BM and of the BM on the RS were measured. Mean values of BM between OC positive versus negative, DJD positive versus negative and DIPJ effusion positive versus negative were compared using SAS statistical program. Results: No effect of the combined BM was found on the RS. No significant differences between the values of the BM were found within the different radiological classes (RC). However, considering only the Coll2-1NO2 marker, the probability to belong to class A was given by the equation 9.63 -0.31 Coll2-1NO2 + 0.04 (Coll2-1NO2)². Equally a tendency (p= 0.06) towards an increase in RC by 0.45 for each increasing unit of Coll2-1NO2 was present. Significant positive correlations were found between Coll2-1 values and Coll2-1 NO2 values. MPO values were found to be significantly higher in OC negative horses (357.86  64.43 ng/ml) than in OC positive horses (231.17  27.15 ng/ml). IGF-I levels were found significantly lower in the positive DIPJ effusion group (416.05  17.57 ng/ml) compared to the negative DIPJ effusion group (477.1  22.40 ng/ml). Coll2-1 values were significantly higher in the positive DIPJ effusion group (911.04  41.13 nM) compared to the negative DIPJ effusion group (769.04  37.92 nM). Mean values of Coll2-1 were significantly higher in the DJD positive group (901.24  36.75 nM) compared to the DJD negative group (790.590  39.397 nM) Discussion: The combination of the blood parameters did not seem to correlate with the used RS system. Coll2-1NO2 levels however tend to increase with poorer RC and could therefore be used as a useful predictor of the osteoarticular status of the horse. Coll2-1 levels were significantly higher in the degenerative joint disease group. A high percentage of horses with DIPJ effusion was present in this study and was associated with decreased IGF-I and increased Coll2-1 levels. [less ▲]

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See detailVariations of plasmatic concentrations of Insulin-like Growth Factor-I in post-pubescent horses affected with developmental osteochondral lesions.
Verwilghen, Denis ULg; Vander Heyden, Laurent ULg; Franck, Thierry ULg et al

in Veterinary Research Communications (2009)

Developmental osteochondral lesions are often encountered in the equine population and are a major cause of lameness. Different growth factors that act systemically as well as locally regulate the growth ... [more ▼]

Developmental osteochondral lesions are often encountered in the equine population and are a major cause of lameness. Different growth factors that act systemically as well as locally regulate the growth of cartilage. Among them is Insulin-like Growth Factor I that has been demonstrated to promote chondrocyte growth and differentiation and that has been shown to influence cartilage repair. The aims of this study were to investigate differences in circulating plasma levels of Insulin-like Growth Factor-I in post-pubescent horses affected with developmental osteochondral lesions compared to unaffected ones. Significantly higher values of circulating Insulin-like Growth Factor-I levels were found in the affected group (n = 82) compared to controls (n = 86). This result may still reflect an earlier imbalance in IGF-I levels from horses with developmental osteochondral lesions considering the aetiopathological link which has been made between IGF-I and the occurrence of osteochondrosis. However, other studies have shown increased expression of IGF-I after cartilage damage. The higher levels found in this study could be due to a healing response of the cartilage to the damage caused by the osteochondral lesions. [less ▲]

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See detailEffect de l'entraînement sur la fonction mitochondriale musculaire du cheval d'endurance
Votion, Dominique ULg; Fraipont, Audrey ULg; Franck, Thierry ULg et al

in 35eme Journee de la Recherche Equine, jeudi 26 fevrier 2009, Paris, France (2009)

Athletic ability of endurance horses is intimately linked to muscle oxidative capacity. Microbiopsies of triceps brachii (TB) and gluteus medius (GM) were collected before and after 10 weeks of training ... [more ▼]

Athletic ability of endurance horses is intimately linked to muscle oxidative capacity. Microbiopsies of triceps brachii (TB) and gluteus medius (GM) were collected before and after 10 weeks of training in 7 endurance horses to assess the feasibility of studying training effect with the use of microbiopsies. Oxygen consumption of permeabilized fibers was evaluated by high resolution respirometry (HRR) with a titration protocol that defines the activity of the mitochondrial complexes. Differences among muscles before and after training as well as training effect were assessed by Wilcoxon matched pairs test (P<0.05). No complications occurred following microbiopsies in any horse. The in situ oxidative capacity of the TB and GM increased with training. No difference was found between the TB and GM when one horse was excluded from the statistical analysis. Indeed, this horse showed signs of exercise intolerance before sampling (after the training period) which were associated to a low rate of respiration in GM but not in TB. Results of this study showed that HRR may be used to follow training effect and suggest that metabolic impairment might be detected with HRR. [less ▲]

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See detailIn vivo administration of acepromazine or promethazine to horse decreases the reactive oxygen species production response of subsequently isolated neutrophils to stimulation with phorbol myristate acetate
Péters, Fabrice; Franck, Thierry ULg; Pequito, Manuel et al

in Journal of Veterinary Pharmacology & Therapeutics (2009)

The previous experiments have shown that some phenothiazines have antioxidant and anti-inflammatory properties in vitro. In this study the inhibition of the production of reactive oxygen species (ROS) by ... [more ▼]

The previous experiments have shown that some phenothiazines have antioxidant and anti-inflammatory properties in vitro. In this study the inhibition of the production of reactive oxygen species (ROS) by neutrophils was studied in two groups of horses, which received a dose of 0.1 mg⁄ kg of either acepromazine or promethazine intravenously. Blood samples were collected before (T0) and 0.5, 1, 3 and 5 h after drug administration. The chemiluminescence (CML) response of neutrophils was measured ex vivo in the presence of luminol for a period of 10 min and the maximum CML value (peak value) recorded. There was a significant inhibition of the ROS production in the acepromazine treated group (49% inhibition) at 5 h after administration and in the promethazine group (24% inhibition) at 3 h after administration (P < 0.05 vs. T0). These findings are of therapeutic relevance in the use of phenothiazines in equine patients with inflammatory diseases where neutrophil activation and ROS production are implicated. [less ▲]

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See detailAtypical Myopathy In Grazing Horses: A First Exploratory Data Analysis
Votion, Dominique ULg; Linden, Annick ULg; Delguste, Catherine ULg et al

in Veterinary Journal (2009), 180(1),

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See detailMethod for co-purification of equine neutrophil elastase and myeloperoxidase from a limited blood volume.
de la Rebière de Pouyade, Geoffroy ULg; Serteyn, Didier ULg; Deby, Ginette ULg et al

in Research in Veterinary Science (2009)

Neutrophil myeloperoxidase (MPO) and elastase can be released in severe inflammatory diseases and cause tissue injuries. Equine enzymes have already been individually purified from large blood quantities ... [more ▼]

Neutrophil myeloperoxidase (MPO) and elastase can be released in severe inflammatory diseases and cause tissue injuries. Equine enzymes have already been individually purified from large blood quantities. We describe the isolation of both enzymes from a same limited blood volume. Both MPO and elastase were extracted by crushing PMN isolated by centrifugation on a percoll-gradient from a 460ml blood collection. MPO and elastase were separated by an ionic exchange chromatography phase and further purified by gel filtration chromatography on Superdex 200 and 75, respectively. Enzymes were identified in the collected fractions by specific enzymatic assays. The final purity was verified by electrophoresis. Specific activity was improved to 19.92 and 34.3x for elastase (final yield: 340mug) and MPO (final yield: 130mug), respectively, during the procedure. Results show the possibility of isolating both enzymes from the same blood sample with a sufficient yield and purity for future studies on their implication and interaction during inflammatory diseases. [less ▲]

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See detailThe unexpected hidden face of the Cephalosporin Antibiotic Ceftazidime: From biological to chemical and physical activities against oxidant species produced by phagocytes
Mouithys-Mickalad, Ange ULg; Deby-Dupont, Ginette; Mathy-Hartert, Marianne ULg et al

Conference (2008, October 03)

Background: Over several decades the use of antibiotics to treat infectious and bacterial diseases has been the main challenge. Ceftazidime (CAZ), belonging to the cephalosporin’s family, is known as ... [more ▼]

Background: Over several decades the use of antibiotics to treat infectious and bacterial diseases has been the main challenge. Ceftazidime (CAZ), belonging to the cephalosporin’s family, is known as empiric treatment for severe sepsis. Beside its antibiotic effect, CAZ has been shown to have other properties, making it unique. This study is aimed to investigate unexpected antioxidant properties of CAZ with special emphasis on the mechanism of action. Methods: Four in vitro and ex-vivo experimental models were designed 1) Assay of proteinases inhibitory activity of α2-macroglobulin (α2M) in the presence of trypsine (1.4µg) with or without 10-3 M CAZ, in 0.1 M Tris-HCl at pH 8.1. 2) Assessment of MPO-induced toxicity on endothelial cells or oxidant activities of stimulated phagocytes (PMNs) using 160 µM ferricytochrome C. 3) Effect of CAZ on two models of anoxia/reoxygenation from adherent and suspension alveolar cells (A549, 106 to 1010 cells/ml) using oxymetry coupled to EPR-spin trapping technique. 4) Cell-free systems to investigate: lipoperoxidation of linoleate induced by γ-irradiation, Fe2+/ascorbate system or ferryl species; Quenching of hydroxyl radical (·OH) and singlet oxygen (1O2)-scavenging activity from Mallet’s (H2O2/NaOCl) and Fenton’ reactions. Results: Ex-vivo assays show efficient protective effect of CAZ on plasmatic antiproteinases against oxidative stress; a dose-dependent inhibitory capacity on endothelial and A549 cells against MPO toxicity or excessive production of ROS during anoxia/reoxygenation. On cell-free systems, CAZ had unique 1O2–scavenging activity; and less effect on ferryl species. CAZ exerts its antioxidant effect by chelating activity. Conclusions: Overall results indicate that: 1) CAZ protects endothelial cells against MPO toxicity but also A549 cells towards the effect of anoxia/reoxygenation; 2) CAZ protects α2M and 3) acts as efficient inhibitor of lipid peroxidation of linoleate and hydroxyl radical and as singlet oxygen-scavengers. Antioxidant properties of CAZ might be relevant in clinical situations where excessive activation of leukocytes is known and in anoxia/reoxygenation model cause an increased production of ROS. [less ▲]

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See detailConduite à tenir face a un cas de myopathie atypique du cheval au pré
Van Galen, Gaby ULg; Serteyn, Didier ULg; Marcillaud Pitel, Chistel et al

(2008, October)

Owing to the systematic recording of epidemiological and clinical data of European cases of atypical myopathy via the « Atypical Myopathy Alert Group » (AMAG) risk factors for AM have been recognized ... [more ▼]

Owing to the systematic recording of epidemiological and clinical data of European cases of atypical myopathy via the « Atypical Myopathy Alert Group » (AMAG) risk factors for AM have been recognized. Preventive measures may be advised from the identified demographic, managemental and environmental risk factors for atypical myopathy. From the clinical course of affected horses and the review of the lately published scientific papers, a symptomatic treatment is proposed as well as a supportive therapy based on the recently identified pathological process involved in atypical myopathy. [less ▲]

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See detailDiagnostic différentiel des troubles musculaires.
van Galen, Gaby; Amory, Hélène ULg; Serteyn, Didier ULg et al

(2008, October)

The horse is particularly prone to myopathies, which can be acquired or inherited. The history and clinical signs of myopathic horses are of major importance for their diagnosis, but they can be quite ... [more ▼]

The horse is particularly prone to myopathies, which can be acquired or inherited. The history and clinical signs of myopathic horses are of major importance for their diagnosis, but they can be quite aspecific, complicating the diagnosis. Often complementary exams, as rectal exploration, locomotory and/or neurological examination, blood and urine analysis, are necessary to distinguish a myopathy from other conditions. When the presence of a myopathy is confirmed, ideally its origin should be determined. The origin can sometimes be suspected by specific elements in the signalment, history and/or complementary exams, but often needs to be confirmed by the histological examination of a muscle biopsy and/or genetic tests. [less ▲]

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See detailLes nouveaux outils d’investigation des myopathies « typiques » et atypique.
Votion, Dominique ULg; van Galen, Gaby; Deby-Dupont, Ginette et al

(2008, October)

This paper aims at describing new investigation tools that are currently under development. Oxymetry, electron spin resonance and myeloperoxidase assays may be applied on muscle sample taken by ... [more ▼]

This paper aims at describing new investigation tools that are currently under development. Oxymetry, electron spin resonance and myeloperoxidase assays may be applied on muscle sample taken by microbiopsy as well as on culture of myocytes. In a next future, these tools should improve our knowledge of the pathophysiological mechanisms involved in clinical and subclinical equine myopathies. [less ▲]

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See detailLa réalité clinique des myopathies équines
Votion, Dominique ULg; van Galen, Gaby; Serteyn, Didier ULg

(2008, October)

Based on histology and the recent genetic studies, several equine neuromuscular disorders have been identified. Lately, several studies have defined the prevalence of myopathies based on histology in ... [more ▼]

Based on histology and the recent genetic studies, several equine neuromuscular disorders have been identified. Lately, several studies have defined the prevalence of myopathies based on histology in equine populations with and without clinical signs of neuromuscular disorders. These studies have showed a high prevalence of myopathies including polysaccharide storage myopathy, recurrent exertional rhabdomyolysis and equine motor neurone disease in horses showing signs of neuromuscular disorders as well as in the general equine population. Based on histology alone, a significant percentage of horses with clinical signs of myopathies remained without a definite diagnosis. In conclusion, horses may be affected by a variety of myopathies, which necessitate an appropriate prophylaxis and a fine diagnostic procedure to avoid the unwanted severe clinical signs associated to muscle disorders. [less ▲]

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