References of "Serteyn, Didier"
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See detailIs neutrophil elastase associated with myeloperoxidase concentration and post-thawing parameters in equine frozen semen?
Ponthier, Jérôme ULg; de la Rebière de Pouyade, Geoffroy ULg; Desvals, Maud et al

in Animal Reproduction Science (2010), 121S

Non sperm cells concentration is higher in unfreezable semen. A relation between non-sperm cells in fresh semen and MPO and post thawing quality has been observed. Neutrophil Elastase seems to have no ... [more ▼]

Non sperm cells concentration is higher in unfreezable semen. A relation between non-sperm cells in fresh semen and MPO and post thawing quality has been observed. Neutrophil Elastase seems to have no effect on semen characteristics and to be not associated with on-sperm cells and freezability. [less ▲]

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See detailLa microbiopsie musculaire : un nouvel outil pour le suivi sportif et la détection précoce des dysfonctions musculaires
Votion, Dominique ULg; Fraipont, Audrey ULg; Robert, Céline et al

in 36ème Journée de la Recherche Equine (2010)

The objective of this study was to confirm the practical value of high-resolution respirometry (HRR) applied to biopsies to determine, in horses, the level of training, their athletic ability and for the ... [more ▼]

The objective of this study was to confirm the practical value of high-resolution respirometry (HRR) applied to biopsies to determine, in horses, the level of training, their athletic ability and for the early detection of muscular dysfunction. Materials and methods – The muscle mitochondrial respiration was determined by HRR in 20 endurance horses and 10 trotters sampled at the triceps brachii at different stages of their training. Results – Training increases mitochondrial respiration, in addition, the best performers had the highest rate of respiration. A trotter had abnormally low levels of muscle mitochondrial respiration for its level of training. This horse has presented several episodes of rhabdomyolysis during its racing season. Discussion – The biopsy is easily achievable by the attending veterinarian. This study shows that the athletic ability of horses is closely linked to respiratory muscle function, and suggests the value of HRR for performance prediction. In addition, the RHR has the ability to demonstrate mitochondrial dysfunction potentially responsible for exercise-induced rhabdomyolysis. [less ▲]

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See detailEuropean outbreak of atypical myopathy in the autumn 2009.
Van Galen, Gaby ULg; Amory, Hélène ULg; Busschers, Evita et al

in Journal of Veterinary Emergency & Critical Care (2010)

Background: Atypical myopathy (AM) is an acute, severe rhabdomyolysis occurring in grazing horses. Since In the beginning of October 2009, a new outbreak affects started in several European countries ... [more ▼]

Background: Atypical myopathy (AM) is an acute, severe rhabdomyolysis occurring in grazing horses. Since In the beginning of October 2009, a new outbreak affects started in several European countries. Geographic, demographic and clinical data of the reported cases in the month October 2009 are described. Key Findings: The survival rate over the month October 2009 was 25%. The most frequently observed clinical signs were congestedive mucous membranes, dyspnea, tachycardia, depression, weakness, stiffness, recumbency, trembling, transpirationsweating, and myoglobinuria. Non-survivors were significantly more often likely to be recumbent than survivors. Prognostic factors, symptomatic treatment and preventive measures are discussed. Significance: Slight differences were encountered during the described outbreak of AM in October 2009 compared to previous studies. Equine practitioners should be alert aware that previous epidemiological studies have shown that after a high prevalence this autumn, for new cases are likely to occur in the upcoming spring 2010. [less ▲]

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See detailEffect of intensive exercise on plasmatic neutrophil elastase level in eventing and endurance horses
Lejeune, Jean-Philippe ULg; Sandersen, Charlotte ULg; Votion, Dominique ULg et al

in Equine Veterinary Journal. Supplement (2010), 48

Reasons for performing the study – Intensive exercise induces a systemic inflammatory response characterized by an increase of blood neutrophil count and myeloperoxidase (MPO) release. Neutrophil elastase ... [more ▼]

Reasons for performing the study – Intensive exercise induces a systemic inflammatory response characterized by an increase of blood neutrophil count and myeloperoxidase (MPO) release. Neutrophil elastase (NE) could also contribute to tissues lesions by their proteinase activities. Objective – To compare plasmatic NE concentrations before and after different forms of intensive exercise. Materials and Methods – EDTA blood samples were taken from 51 eventing horses (EvH) and 32 endurance horses (EndH) were sampled before the race (T0). Blood sampling was performed 2 h (T1) after completing either phase D of a one or two star eventing competition (n=51) or a 120 or 160 km endurance race (n=32). Plasmatic NE and MPO were measured by a specific equine ELISA. Neutrophil counts and creatine kinase (CK) levels were also measured. A Wilcoxon test for paired samples was used to compare mean values of neutrophils, CK, MPO and NE at T0 and T1 in EvH and in EndH. Correlations were calculated between all the 4 parameters in EvH and EndH. Results – At T0, mean NE levels were 14.43 ± 3.63 ng/ml for EvH and 11.7 ± 2.11 ng/ml for EndH. The competition induced a significant increase of NE levels in (58.57 ± 24.06 ng/mL) EvH and (95.74 ± 22.70 ng/mL) EndH (p < 0,05). NE was significantly (p < 0,0001) correlated to MPO in EvH (r = 0.293) and EndH (0.594) and to CK (r = 0.297) in EndH (p<0.0001). Neutrophils, CK and MPO were significantly increased between T0 and T1 in both types of horses. Conclusions – Significant increase of NE was observed after intense exercise with a significant correlation between NE and MPO. The huge variability in MPO and ELT, indicates, that not all horses show the same intensity of systemic inflammatory response. [less ▲]

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See detailAlterations in mitochondrial respiratory function in response to endurance training and endurance racing
Votion, Dominique ULg; Fraipont, Audrey ULg; Goachet, Anne-Gaëlle et al

in Equine Veterinary Journal. Supplement (2010), 42(38), 268-274

Objectives: To determine effects of training and racing on muscle oxidative phosphorylation (OXPHOS) and electron transport system (ETS) capacities in horses with high-resolution respirometry (HRR).

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See detailPrevalence of radiographic findings in candidate sires (2001-2008)
Verwilghen, Denis ULg; Serteyn, Didier ULg; Pille, Frederik et al

in Vlaams Diergeneeskundig Tijdschrift (2009), 78

Radiographic screening of horses prior to purchase or admission to studbooks has become an important tool for evaluating the osteo-articular status of the animals. Developmental orthopedic disorders (DOD ... [more ▼]

Radiographic screening of horses prior to purchase or admission to studbooks has become an important tool for evaluating the osteo-articular status of the animals. Developmental orthopedic disorders (DOD) are the main cause of potential lameness in youngsters. The genetic factor associated with DOD has been established, and specific selection schemes have been developed to diminish the prevalence of lesions. Increasing knowledge of prevalence in different breeds may help in developing specific breeding strategies. This article presents the results of the radiographic screening of 676 stallions presented for sire admission to the Royal Belgian Sports Horse Society (Studbook sBs). On average, two radiographic findings were noted per horse. The prevalence of DOD in this population was 38.9%. A high percentage of horses showed radiographic signs of effusion of the distal interphalangeal joint. An uncommon fragment location at the proximal tubercle of the talus was found in 7 horses. The present paper presents a review and discussion of all the radiographic findings. [less ▲]

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See detailLes tendinopathies et desmopathies de la région métacarpienne et –tarsienne: une revue des thérapies actuelles. Première partie : la structure, les lésions du tendon et le diagnostic.
Verwilghen, Denis ULg; Gabriel, Annick ULg; Busoni, Valeria ULg et al

in Annales de Médecine Vétérinaire (2009), 3(153), 145-155

Lesions to the horse’s tendon are very frequent and are a non negligible reason for early retirement of sport and leisure horses. For decades man has been searching for “the” treatment but unfortunately ... [more ▼]

Lesions to the horse’s tendon are very frequent and are a non negligible reason for early retirement of sport and leisure horses. For decades man has been searching for “the” treatment but unfortunately most of the known treatments have empiric bases and very few have actually proven their real efficiency. Better knowledge of tendon anatomy and pathophysiology of tendonitis together with evolutions in diagnostic techniques like ultrasonography allows us to better evaluate the improvements made in tendonitis therapies today. The first part of this article gives a review on tendon structure, tendon healing and ultrasonographic evaluation of the tendon. In the second part an overview of therapeutic options for tendonitis will be discussed. [less ▲]

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See detailAMADEOX method : combination of various techniques to distinguish stoichiometric and anticatalytic activities of antioxidant molecules or natural extracts
Franck, Thierry ULg; Mouithys-Mickalad, Ange ULg; Kohnen, Stephan et al

in International Society of Antioxidants in Nutrition & Health, 5th International Conference on Polyphenols Applications, Bridging Bioefficacy to Innovations & Applications, October 29th-30th 2009, Malta (2009, October 29)

We developed an original method called AMADEOX (for « Action Modulatrice de l'Activité Des Enzymes Oxydantes ») able to distinguish stoichiometric, metabolic and anticatalytic activities of antioxidant ... [more ▼]

We developed an original method called AMADEOX (for « Action Modulatrice de l'Activité Des Enzymes Oxydantes ») able to distinguish stoichiometric, metabolic and anticatalytic activities of antioxidant molecules or natural extracts on stimulated neutrophils and purified myeloperoxidase (MPO), a key enzyme released by neutrophil and acting as one of the major ROS generating systems. [less ▲]

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See detailEvolution of blood myeloperoxidase in the perioperative period of horses undergoing emergency celiotomy.
Salciccia, Alexandra ULg; Grulke, Sigrid ULg; de la Rebière de Pouyade, Geoffroy ULg et al

Poster (2009, July)

Colic can cause an activation of neutrophils with release in the blood flow of myeloperoxidase (MPO), a specific enzyme with strong oxidative activity. The aim of this study was to describe the evolution ... [more ▼]

Colic can cause an activation of neutrophils with release in the blood flow of myeloperoxidase (MPO), a specific enzyme with strong oxidative activity. The aim of this study was to describe the evolution of plasma MPO after colic surgery. Materials included 13 adult horses that underwent an emergency celiotomy for acute intestinal obstruction. Venous blood samples were collected into EDTA anticoagulated tubes before surgery, during surgery after correction of the intestinal lesion and during the recovery of anaesthesia. In the postoperative period samples were taken every 4 hours during the first 4 days (from day 0 until day 4), every 12 hours during the days 4 and 5 and every 24 hours until day 10. MPO was assayed with a specific enzyme-linked immunosorbent assay. Statistical analysis was performed by one way Anova with student- Newman-Keuls post test on data obtained for each time point. Significance was set at p < 0.05. The horses were operated for an obstruction strangulated or not of the small or the large intestine. In six cases the postoperative period was uneventful, the 7 remaining developed one or 2 severe complications. Eight horses were discharged and 5 died during the hospitalization.The general aspect of the curve of mean plasmatic MPO can be described as follow: An increase was observed from the admission on until a peak of concentration occurring generally during the time of recovery from the anaesthesia with the highest mean value reaching 740.84 +/- 507.61ng/ml. This was followed by a progressive decrease until the lowest value, usually near to day 2 after the recovery from anaesthesia corresponding to 171.79 +/- 76.21 ng/ml of MPO. Afterwards, the mean concentrations increased slowly until postoperative day 10. In the majority of cases a stable and low MPO value (plateau) was observed during approximately 2 days (from day 1 to day 3 postoperatively).The initial peak of MPO after surgery could be associated to the neutrophil activation consequent to the intestinal disorder and the intense stimulation of the coeliotomy. The following significant reduction in concentration could be attributed to MPO infiltration into the tissues with a critical point at approximately 2 days after surgery. This study may contribute to a better understanding of the role of the MPO and neutrophils in the pathophysiology of horses in the postoperative period after colic surgery. [less ▲]

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See detailEstimation of joint contact forces in the equine digit
Noble, Prisca ULg; Collin, Bernard ULg; Denoix, Jean-Marie et al

in ISB proceedings (2009, July)

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See detailMuscle mitochondrial respiratory function in healthy and myopathic horses
Votion, Dominique ULg; Serteyn, Didier ULg

in In Proceedings (CD-ROM): Mitochondrial Medicine 2009: Capitol Hill Symposium (2009, June)

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See detailPreparation and application of permeabilized muscle fibers for respiration studies
Votion, Dominique ULg; Pesta, Dominik; Gnaiger, Erich et al

in In Proceedings: 3rd MiPsummer School (2009, June)

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See detailHigh-resolution respirometry to assess muscle mitochondrial respiratory function in horses: potential applications in sport and myopathic horses
Votion, Dominique ULg; Gnaiger, Erich; Lemieux, Hélène et al

in In Proceedings: 3rd MiPsummer School, Baton Rouge, USA (2009, June)

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See detailEvaluation of low-molecular-weight heparin for the prevention of equine laminitis after colic surgery
de la Rebière de Pouyade, Geoffroy ULg; Grulke, Sigrid ULg; Detilleux, Johann ULg et al

in Journal of Veterinary Emergency and Critical Care (2009), 19(1), 113-119

Objectives – The aim of this study is to describe the prevalence of postoperative laminitis in colic cases and to determine if low-molecular-weight heparin (LMWH) is effective in preventing this ... [more ▼]

Objectives – The aim of this study is to describe the prevalence of postoperative laminitis in colic cases and to determine if low-molecular-weight heparin (LMWH) is effective in preventing this complication. Design – Retrospective clinical study. Animals – Client-owned horses. Interventions – SC administration of enoxaparin during the postoperative period. Measurements and Main Results – Medical records of 360 horses undergoing surgery for colic and surviving at least 3 days were evaluated. Fifty-six horses admitted before 1995 did not receive LMWH (control group) and 304 admitted after 1995 received LMWH as a prophylaxis for laminitis (treatment group). Three grades of severity were defined for laminitis. Prevalence and severity of laminitis were compared between the 2 groups. Several parameters recorded on admission (sex, age, breed, site and nature of the disease, heart rate, PCV, gravity score, and shock score) and the administration of LMWH were tested as risk factors in the development of laminitis in a logistic regression procedure. Prevalence and grade of laminitis were significantly lower in the treatment group. Only the absence of LMWH was recognized as a significant risk factor in the logistic regression model. Conclusions – The administration of LMWH appears to be effective in the prophylaxis of laminitis following colic surgery and may be useful in the postoperative management of these horses. [less ▲]

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See detailCombined method for the sequential measurement of (1) the enzymatically active fraction and (2) the total amount of an enzyme
Serteyn, Didier ULg; Deby-Dupont, Ginette; Franck, Thierry ULg et al

Patent (2009)

The present invention is related to a combined method and kit (or device) for the sequential measurement of the enzymatically active fraction and the total amount of an enzyme [(such as myeloperoxidase ... [more ▼]

The present invention is related to a combined method and kit (or device) for the sequential measurement of the enzymatically active fraction and the total amount of an enzyme [(such as myeloperoxidase (MPO)] in a sample, and that find improved applications in veterinary and human health fields. [less ▲]

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See detailRelationship between biochemical markers and radiographic scores in the evaluation of the osteoarticular status of Warmblood stallions
Verwilghen, Denis ULg; Busoni, Valeria ULg; Gangl, Monika et al

in Research in Veterinary Science (2009), 87(2), 319-28

Establishing the osteoarticular status of the horse is often performed by means of radiological screening of the animals. Widespread blood sampling could reveal to be an interesting alternative to this ... [more ▼]

Establishing the osteoarticular status of the horse is often performed by means of radiological screening of the animals. Widespread blood sampling could reveal to be an interesting alternative to this procedure which is time consuming and sometimes technically difficult. The aim of this study was to investigate the relationship between the radiological status of the horses and the levels of biochemical markers of cartilage degradation and synovial inflammation. A specific radiological scoring and classification system was therefore developed and applied on 63 stallions presented for studbook admission. Additionally, groups of horses were established according to the occurrence of osteochondrosis, degenerative joint disease and distal interphalangeal joint effusion. Insulin growth factor-I, myeloperoxidases, Coll2-1 and Coll2-1NO(2) were used as blood markers. The combination of the blood parameters did not seem to correlate with the used scoring system. Coll2-1NO(2) levels however tended to increase with poorer radiological class and this could therefore potentially be a useful predictor of the osteoarticular status in the horse. Coll2-1 levels were significantly higher in the degenerative joint disease group. A high percentage of horses with distal interphalangeal joint effusion was present in this study and was associated with decreased IGF-I and increased Coll2-1 levels. [less ▲]

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