References of "Scippo, Marie-Louise"
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See detailViande « enrichie en oméga-3 » : quel risque pour la santé ?
Scippo, Marie-Louise ULg

Conference (2009, November 05)

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See detailAdaptation of a microbiological method for screening antimicrobial residues in shrimp tissue
Pham Kim, Dang ULg; Degand, Guy ULg; Danyi, Sophie ULg et al

in Book of abstracts - 4th International Symposium on Recent Advances in Food Analysis (RAFA 2009) (2009, November 04)

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See detailBehavior of omega-3 fatty acids in eggs during cooking
Douny, Caroline ULg; El Khoury, Rawad ULg; Degand et al

Poster (2009, July 01)

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See detailMilk collection and processing
Scippo, Marie-Louise ULg

Conference (2009, February 23)

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See detailDevelopment of a quantitative method to detect trace amounts of hazelnut and soy allergens in food
Dobson, Rowan ULg; Fourdrilis, Séverine; Kirsch, Stéphanie ULg et al

Conference (2009)

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See detailDEVELOPMENT OF AN INTEGRATED STRATEGY FOR CONTROLLING THE ALLERGEN ISSUE IN THE BELGIAN FOOD AND CATERING INDUSTRY - ALLERRISK
De Loose, Marc; Daeseleire, Else; Taverniers, Isabelle et al

Report (2009)

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See detailQuantitative methods for food allergens: a review.
Kirsch, Stéphanie ULg; Fourdrilis, Séverine ULg; Dobson, Rowan ULg et al

in Analytical and Bioanalytical Chemistry (2009), 395

The quantitative detection of allergens in the food chain is a strategic health objective as the prevalence of allergy continues to rise. Food allergenicity is caused by proteins either in their native ... [more ▼]

The quantitative detection of allergens in the food chain is a strategic health objective as the prevalence of allergy continues to rise. Food allergenicity is caused by proteins either in their native form or in forms resulting from food processing. Progress in mass spectrometry greatly opened up the field of proteomics. These advances are now available for the detection and the quantification of traces of allergenic proteins in complex mixtures, and complete the set of biological tests used until now, such as ELISA or PCR. We review methods classified according to their ability to simultaneously quantify and identify allergenic proteins and underline major advances in the mass-spectrometric methods. [less ▲]

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See detailEvaluation of the endorcine disrupting chemicals contamination in the North Sea porpoise population using in vitro assays
Didimo Imazaki, Pedro Henrique ULg; Brose, François ULg; Van Der Heiden, Edwige ULg et al

Poster (2009)

During the last decades, the production endocrine disrupting chemicals reached such levels that they are now spread all over nature. They are known to be very slowly degraded, decreasing the environmental ... [more ▼]

During the last decades, the production endocrine disrupting chemicals reached such levels that they are now spread all over nature. They are known to be very slowly degraded, decreasing the environmental quality and posing ecological risks. Marine mammals inhabiting polluted environments accumulate high levels of these chemicals, so they can be considered good indicators of marine pollution. Thirteen major organochloride pollutants were chosen to make subject of this study: o,p’-DDD; p,p’-DDD; p,p’-DDE; o,p’-DDT; p,p’-DDT; HCB; α-HCH; β-HCH; γ-HCH; δ-HCH; PCB 138; PCB 153 and PCB 180. All these chemicals will have their endocrine disrupting effects characterized individually and collectively by report gene expression assays. The MCF7-ERE cells used in these assays were produced in the Molecular Biology and Genetic Engineering Laboratory of the University of Liège and are originated from a human mammary carcinoma. They carry a gene expressing the synthesis of luciferase and controlled by oestrogen receptors. Then, the thirteen chemicals will be searched in the blubber tissues of porpoises by the use of gas chromatography-mass spectrometry. The blubber samples will also have their endocrine disrupting effect characterized. For the moment, the estrogenic effects of the HCH isomers were put in evidence. The next steps of this work must be soon accomplished. It is expected a great level of these compounds to be found in the samples. On the other hand, their endocrine disrupting effects and how they act in agonist, antagonist and synergist ways are subject that still needs to be cleared. [less ▲]

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See detailEvaluation of the contamination of North Sea Porpoises (Phocoena phocoena) by endocrine disrupting chemicals using CG-MS and in vitro assays
Didimo Imazaki, Pedro Henrique ULg; Brose, François ULg; Remacle, Anne-Sophie ULg et al

Poster (2009)

During the last decades, the production of endocrine disrupting chemicals reached such elevated levels that they are now spread all over the environment. Endocrine disrupting chemicals are known to be ... [more ▼]

During the last decades, the production of endocrine disrupting chemicals reached such elevated levels that they are now spread all over the environment. Endocrine disrupting chemicals are known to be very slowly degraded, decreasing the environmental quality and causing ecological risks. Marine mammals inhabiting polluted environments accumulate high quantities of these chemicals, and are good indicators of marine pollution Thirteen major organochloride pollutants (known from literature to contaminate north sea porpoise) were chosen in this study – o,p’-DDD; p,p’-DDD; p,p’-DDE; o,p’-DDT; p,p’-DDT; HCB; α-HCH; β-HCH; γ-HCH; δ-HCH; PCB 138; PCB 153 and PCB 180. The thirteen chemicals were tested individually and in mixtures by the use of report gene expression assays. The MCF7-ERE cells used in the assays were originated from MCF7 human mammary tumor cells transfected with the ERE-luciferase reporter system. Preliminary results suggest that o,p’-DDD; p,p’-DDD; o,p’-DDT; HCB; β-HCH; δ-HCH; PCB 138 and PCB 180 are able to stimulate the luciferase expression of MCF7-ERE cells when they are present individually or in mixtures. No synergism was observed within mixtures. When MCF7-ERE cells were exposed to the chemicals and 17β-oestradiol simultaneously, p,p’-DDE; α-HCH; PCB 138 and PCB 180 could raise the luciferase expression in comparison to the exposition of 17β-oestradiol alone; on the other hand, o,p’-DDD; p,p’-DDT; HCB; β-HCH; γ-HCH and δ-HCH seem to inhibit the luciferase expression at low doses. In the next step, the pollutants will be searched and quantified in the blubber tissue of porpoises by use of gas chromatography-mass spectrometry. The blubber tissue samples will also have their endocrine disrupting effects characterized. This work is ongoing and It is expected a relevant level of these compounds to be found in the samples. Conversely, their endocrine disrupting effects and how they act in agonist, antagonist ways are subject that still needs to be studied. [less ▲]

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