References of "Scippo, Marie-Louise"
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See detailMilk collection and processing
Scippo, Marie-Louise ULg

Conference (2009, February 23)

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See detailDevelopment of a quantitative method to detect trace amounts of hazelnut and soy allergens in food
Dobson, Rowan ULg; Fourdrilis, Séverine; Kirsch, Stéphanie ULg et al

Conference (2009)

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See detailDEVELOPMENT OF AN INTEGRATED STRATEGY FOR CONTROLLING THE ALLERGEN ISSUE IN THE BELGIAN FOOD AND CATERING INDUSTRY - ALLERRISK
De Loose, Marc; Daeseleire, Else; Taverniers, Isabelle et al

Report (2009)

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See detailQuantitative methods for food allergens: a review.
Kirsch, Stéphanie ULg; Fourdrilis, Séverine ULg; Dobson, Rowan ULg et al

in Analytical and Bioanalytical Chemistry (2009), 395

The quantitative detection of allergens in the food chain is a strategic health objective as the prevalence of allergy continues to rise. Food allergenicity is caused by proteins either in their native ... [more ▼]

The quantitative detection of allergens in the food chain is a strategic health objective as the prevalence of allergy continues to rise. Food allergenicity is caused by proteins either in their native form or in forms resulting from food processing. Progress in mass spectrometry greatly opened up the field of proteomics. These advances are now available for the detection and the quantification of traces of allergenic proteins in complex mixtures, and complete the set of biological tests used until now, such as ELISA or PCR. We review methods classified according to their ability to simultaneously quantify and identify allergenic proteins and underline major advances in the mass-spectrometric methods. [less ▲]

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See detailEvaluation of the endorcine disrupting chemicals contamination in the North Sea porpoise population using in vitro assays
Didimo Imazaki, Pedro Henrique ULg; Brose, François ULg; Van Der Heiden, Edwige ULg et al

Poster (2009)

During the last decades, the production endocrine disrupting chemicals reached such levels that they are now spread all over nature. They are known to be very slowly degraded, decreasing the environmental ... [more ▼]

During the last decades, the production endocrine disrupting chemicals reached such levels that they are now spread all over nature. They are known to be very slowly degraded, decreasing the environmental quality and posing ecological risks. Marine mammals inhabiting polluted environments accumulate high levels of these chemicals, so they can be considered good indicators of marine pollution. Thirteen major organochloride pollutants were chosen to make subject of this study: o,p’-DDD; p,p’-DDD; p,p’-DDE; o,p’-DDT; p,p’-DDT; HCB; α-HCH; β-HCH; γ-HCH; δ-HCH; PCB 138; PCB 153 and PCB 180. All these chemicals will have their endocrine disrupting effects characterized individually and collectively by report gene expression assays. The MCF7-ERE cells used in these assays were produced in the Molecular Biology and Genetic Engineering Laboratory of the University of Liège and are originated from a human mammary carcinoma. They carry a gene expressing the synthesis of luciferase and controlled by oestrogen receptors. Then, the thirteen chemicals will be searched in the blubber tissues of porpoises by the use of gas chromatography-mass spectrometry. The blubber samples will also have their endocrine disrupting effect characterized. For the moment, the estrogenic effects of the HCH isomers were put in evidence. The next steps of this work must be soon accomplished. It is expected a great level of these compounds to be found in the samples. On the other hand, their endocrine disrupting effects and how they act in agonist, antagonist and synergist ways are subject that still needs to be cleared. [less ▲]

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See detailEvaluation of the contamination of North Sea Porpoises (Phocoena phocoena) by endocrine disrupting chemicals using CG-MS and in vitro assays
Didimo Imazaki, Pedro Henrique ULg; Brose, François ULg; Remacle, Anne-Sophie ULg et al

Poster (2009)

During the last decades, the production of endocrine disrupting chemicals reached such elevated levels that they are now spread all over the environment. Endocrine disrupting chemicals are known to be ... [more ▼]

During the last decades, the production of endocrine disrupting chemicals reached such elevated levels that they are now spread all over the environment. Endocrine disrupting chemicals are known to be very slowly degraded, decreasing the environmental quality and causing ecological risks. Marine mammals inhabiting polluted environments accumulate high quantities of these chemicals, and are good indicators of marine pollution Thirteen major organochloride pollutants (known from literature to contaminate north sea porpoise) were chosen in this study – o,p’-DDD; p,p’-DDD; p,p’-DDE; o,p’-DDT; p,p’-DDT; HCB; α-HCH; β-HCH; γ-HCH; δ-HCH; PCB 138; PCB 153 and PCB 180. The thirteen chemicals were tested individually and in mixtures by the use of report gene expression assays. The MCF7-ERE cells used in the assays were originated from MCF7 human mammary tumor cells transfected with the ERE-luciferase reporter system. Preliminary results suggest that o,p’-DDD; p,p’-DDD; o,p’-DDT; HCB; β-HCH; δ-HCH; PCB 138 and PCB 180 are able to stimulate the luciferase expression of MCF7-ERE cells when they are present individually or in mixtures. No synergism was observed within mixtures. When MCF7-ERE cells were exposed to the chemicals and 17β-oestradiol simultaneously, p,p’-DDE; α-HCH; PCB 138 and PCB 180 could raise the luciferase expression in comparison to the exposition of 17β-oestradiol alone; on the other hand, o,p’-DDD; p,p’-DDT; HCB; β-HCH; γ-HCH and δ-HCH seem to inhibit the luciferase expression at low doses. In the next step, the pollutants will be searched and quantified in the blubber tissue of porpoises by use of gas chromatography-mass spectrometry. The blubber tissue samples will also have their endocrine disrupting effects characterized. This work is ongoing and It is expected a relevant level of these compounds to be found in the samples. Conversely, their endocrine disrupting effects and how they act in agonist, antagonist ways are subject that still needs to be studied. [less ▲]

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See detailCYP1A1 and CYP3A4 modulation by dietary flavonoids in human intestinal Caco-2 cells.
Sergent, Thérèse; Dupont, Isabelle; Van Der Heiden, Edwige ULg et al

in Toxicology Letters (2009), 191

Flavonoids have been proposed to exert beneficial effects in a multitude of disease states. However, evidence of potential toxic actions has also emerged. Since large doses of flavonoids can be ... [more ▼]

Flavonoids have been proposed to exert beneficial effects in a multitude of disease states. However, evidence of potential toxic actions has also emerged. Since large doses of flavonoids can be encountered in the intestine simultaneously with ingested drugs and pollutants, this study aimed at investigating nine individual flavonoid compounds and their interactions with the major intestinal isoforms of cytochrome P450, i.e. CYPs 1A1 and 3A4, using human intestinal Caco-2 cells cultivated in a serum-free medium. Genistein, quercetin and chrysin provoked a dose-dependent inducing effect on the CYP1A1 activity, measured with the EROD assay. However, they did not affect the CYP1A1 mRNA expression, suggesting they are not aryl hydrocarbon receptor-ligands in intestinal cells and act at a post-transcriptional level. Chrysin, at 50muM, was detected as a potent inhibitor of the TCDD-induced CYP1A1 activity, leading the activity to ca. 10% of the TCDD-control value (n=3), this effect involving, at least partly, direct interactions at the enzyme level. Quercetin was also shown to significantly inhibit the constitutive CYP3A4 activity, measured by the 6beta-(OH)-testosterone assay, and to impair its induction by 1,25-vitamin D(3). Chrysin, quercetin and genistein, were detected as significant inhibitors of the 1,25-vitamin D(3)-induced CYP3A4 activity. In vivo, these effects could result in reduced activation of procarcinogens and/or in drug bioavailability limitation. They underline the importance of intestinal studies to assess food safety and health risks linked to the ingestion of flavonoid-enriched supplements or functional foods. [less ▲]

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See detailAdverse effects of enrofloxacin when associated with environmental stress in Tra catfish (Pangasianodon hypophthalmus).
Wang, Neil; Nkejabega, Noemie; Hien, Nguyen-Ngoc et al

in Chemosphere (2009), 77(11), 1577-84

The aim of this study was to assess the adverse effects of enrofloxacin (EF) on Tra catfish, Pangasianodon hypophthalmus, in relation with density stress. Fish were held at 40, 80 or 120 fish m(-3) and ... [more ▼]

The aim of this study was to assess the adverse effects of enrofloxacin (EF) on Tra catfish, Pangasianodon hypophthalmus, in relation with density stress. Fish were held at 40, 80 or 120 fish m(-3) and fed with pellets containing either 1 g kg(-1) EF or no EF. Antibiotic exposure lasted 7d and all fish were fed without EF for another 7-d recovery period. Fish were sampled at 3, 7, 8, 10 and 14 d after the beginning of EF exposure. Lipid peroxidation (LPO) and total glutathione (GSH) levels, catalase (CAT), glutathione-s-transferase (GST) and acetylcholine-esterase (AChE) activities were assessed in gill, brain, liver and muscle. At day 7, LPO levels in gills of EF-fish reared at low or high density were significantly more than 5-fold higher than their respective control. On the contrary, LPO in gills of EF-fish reared at medium density was significantly 3-fold lower than the control fish. Similarly, CAT activities in gills of EF-fish reared under low or high density were higher than in their control groups, while this activity was lower in EF-fish of the medium density group. AChE activities in muscles of EF-fish reared at low or high density were lower than controls at days 3 and 7, respectively. These results suggest that EF exposure may lead to disorders like lipid peroxidation and neural dysfunction in fish. However, when reared under lower stress condition (medium density), they may cope better with EF-induced stress than chronically stressed fish (low or high density). [less ▲]

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See detailCYP1A1 induction and CYP3A4 inhibition by the fungicide imazalil in the human intestinal Caco-2 cells-Comparison with other conazole pesticides
Sergent, Thérèse; Dupont, Isabelle; Jassogne, Coralie et al

in Toxicology Letters (2009), 184(3), 159-168

Imazalil (IMA) is a widely used imidazole-antifungal pesticide and. therefore. a food contaminant. This compound is also used as a drug (enilconazole). As intestine is the first site of exposure to ... [more ▼]

Imazalil (IMA) is a widely used imidazole-antifungal pesticide and. therefore. a food contaminant. This compound is also used as a drug (enilconazole). As intestine is the first site of exposure to ingested drugs and pollutants, we have investigated the effects of IMA, at realistic intestinal concentrations, on xenobiotic-metabolizing enzymes and efflux pumps by using Caco-2 cells, as a validated in vitro model of the human intestinal absorptive epithelium. For comparison, other conazole fungicides, i.e. ketoconazole, propiconazole and tebuconazole. were also studied. IMA induced cytochrome P450 (CYP) 1A1 activity to the same extent as benzo(a)pyrene (B(a)P) or 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD), in a dose-and time-dependent manner. Cell-free aryl hydrocarbon receptor (AhR) binding assay and reporter gene assay suggested that IMA is not an AhR-ligand, implying that IMA-mediated induction should involve an AhR-independent pathway. Moreover, IMA strongly inhibited the CYP3A4 activity in 1,25-vitamin D-3-induced Caco-2 cells. The other fungicides had weak or nil effects on CYP activities. Study of the apical efflux pump activities revealed that ketoconazole inhibited both P-glycoprotein (Pgp) and multidrug resistance-associated protein 2 (MRP-2) or breast cancer resistance protein (BCRP), whereas IMA and other fungicides did not. Our results imply that coingestion of IMA-contaminated food and CYP3A4- or CYP1A1-metabolizable drugs or chemicals could lead to drug bioavailability modulation or toxicological interactions, with possible adverse effects for human health. (C) 2008 Elsevier Ireland Ltd. All rights reserved. [less ▲]

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