Screening, post-screening and confirmation analysis of antibiotics in pork and chicken meat marketed in Vietnam.
; Degand, Guy ; Douny, Caroline et al
in EuroResidue VII: conference on residues of veterinary drugs in food (2012)Detailed reference viewed: 42 (9 ULg)
Estimation of furan contamination across the Belgian food chain.
Scholl, Georges ; Scippo, Marie-Louise ; De Pauw, Edwin et al
in Food Additives & Contaminants. Part A. Chemistry, Analysis, Control, Exposure & Risk Assessment (2012), 29(2), 172-9
This paper provides an estimate of the furan content of Belgian foods. The objective of the study was to achieve the best food chain coverage with a restricted number of samples (n = 496). The geographic ... [more ▼]
This paper provides an estimate of the furan content of Belgian foods. The objective of the study was to achieve the best food chain coverage with a restricted number of samples (n = 496). The geographic distribution, different market chains and labels, and consumption frequencies were taken into account in the construction of the sampling plan. Weighting factors such as contamination levels, consumption frequency and the diversity of food items were applied to set up the model. The very low detection capabilities (CC(beta)) of the analytical methods used (sub-ppb) allowed reporting of 78.2% of the overall dataset above CC(beta) and, in particular, 96.7% for the baby food category. The highest furan levels were found in powdered roasted bean coffee (1912 microg kg(-1)) with a mean of 756 microg kg(-1) for this category. Prepared meat, pasta and rice, breakfast cereals, soups, and baby food also showed high mean furan contents ranging from 16 to 43 microg kg(-1). Comparisons with contamination surveys carried out in other countries pointed out differences for the same food group and therefore contamination levels are related to the geographical origin of food items. [less ▲]Detailed reference viewed: 62 (19 ULg)
Treatment of estrogens and androgens in dairy wastewater by a constructed wetland system.
; ; et al
in Water Research (2012), 46(7), 2333-43
Constructed wetland systems (CWS) have been used as a low cost bio-filtration system to treat farm wastewater. While studies have shown that CWS are efficient in removing organic compounds and pathogens ... [more ▼]
Constructed wetland systems (CWS) have been used as a low cost bio-filtration system to treat farm wastewater. While studies have shown that CWS are efficient in removing organic compounds and pathogens, there is limited data on the presence of hormones in this type of treatment system. The objective of this study was to evaluate the ability of the CWS to reduce estrogenic and androgenic hormone concentration in dairy wastewater. This was achieved through a year long study on dairy wastewater samples obtained from a surface flow CWS. Analysis of hormonal levels was performed using a solid phase extraction (SPE) sample clean-up method, combined with reporter gene assays (RGAs) which incorporate relevant receptors capable of measuring total estrogenic or androgenic concentrations as low as 0.24 ng L(-1) and 6.9 ng L(-1) respectively. Monthly analysis showed a mean removal efficiency for estrogens of 95.2%, corresponding to an average residual concentration of 3.2 ng L(-1) 17beta-estradiol equivalent (EEQ), below the proposed lowest observable effect concentration (LOEC) of 10 ng L(-1). However, for one month a peak EEQ concentration of 115 ng L(-1) was only reduced to 18.8 ng L(-1). The mean androgenic activity peaked at 360 ng L(-1) and a removal efficiency of 92.1% left an average residual concentration of 32.3 ng L(-1) testosterone equivalent (TEQ). The results obtained demonstrate that this type of CWS is an efficient system for the treatment of hormones in dairy wastewater. However, additional design improvements may be required to further enhance removal efficiency of peak hormone concentrations. [less ▲]Detailed reference viewed: 69 (15 ULg)
In vivo and in vitro estrogenic activity of the antidepressant fluoxetine.
; Didimo Imazaki, Pedro Henrique ; et al
in Reproductive Toxicology (2012), 34(1), 80-85
Recent years have seen an increase in the use of antidepressant drugs, especially fluoxetine (FLX), in sensitive populations, such as pregnant and lactating women. Although some evidence suggests a ... [more ▼]
Recent years have seen an increase in the use of antidepressant drugs, especially fluoxetine (FLX), in sensitive populations, such as pregnant and lactating women. Although some evidence suggests a possible endocrine action of FLX, no specific studies have been performed to investigate this hypothesis. In the present study, we investigated the possible (anti)androgenic and (anti)estrogenic actions of FLX using Hershberger, uterotrophic (0.4, 1.7, and 17mg/kg), and reporter gene (7.6-129muM) assays. In the Hershberger assay, no differences were observed in androgen-dependent organ weights. However, the uterotrophic and gene reporter assays indicated a possible estrogenic action of FLX. Uterine weight increased in the 1.7 and 17mg/kg/day groups in the 3-day uterotrophic assay in immature rats. Additionally, noncytotoxic concentrations of FLX induced estrogenic responses and increased the estrogenic response of estradiol in MCF-7 breast cancer cells transfected with luciferase. [less ▲]Detailed reference viewed: 31 (20 ULg)
Development of a LC-UV-MS analytical method for aldehydes formed during fatty acids degradation
Douny, Caroline ; ; Brose, François et al
Poster (2012)Detailed reference viewed: 17 (3 ULg)
Étude de l’évolution de l’oxydation lipidique du Tilapia du Nil (Oreochromis niloticus) conservé à température ambiante (30°C) et à (4°C), en Algérie.
; ; Douny, Caroline et al
Poster (2012)Detailed reference viewed: 62 (15 ULg)
Analyse sensorielle et biochimique du Tilapia du Nil (Oreochromis niloticus) conservé à température ambiante (30°C) et à 4°C
; ; Douny, Caroline et al
Poster (2012)Detailed reference viewed: 56 (8 ULg)
KINETICS OF QUINALPHOS RESIDUES ELIMINATION IN WATER AND FISH TISSUES FROM TANK CULTURED SILVER BARB (Barbonymus gonionotus)
; ; et al
Poster (2012)Detailed reference viewed: 39 (3 ULg)
Selection and characterization of PCB-binding DNA aptamers.
; ; et al
in Analytical Chemistry (2012), 84(3), 1669-76
Polychlorinated biphenyls (PCBs) are persistent organic pollutants (POPs) that resist natural degradation and bioaccumulate in nature. Combined with their toxicity, this leads them to cause cancer and ... [more ▼]
Polychlorinated biphenyls (PCBs) are persistent organic pollutants (POPs) that resist natural degradation and bioaccumulate in nature. Combined with their toxicity, this leads them to cause cancer and other health hazards. Thus, there is a vital need for rapid and sensitive methods to detect PCB residues in food and in the environment. In this study, PCB-binding DNA aptamers were developed using PCB72 and PCB106 as targets for aptamer selection. Aptamers are synthetic DNA recognition elements which form unique conformations that enable them to bind specifically to their targets. Using in vitro selection techniques and fluorometry, an aptamer that binds with nanomolar affinity to both the PCBs has been developed. It displayed high selectivity to the original target congeners and limited affinity toward other PCB congeners (105, 118, 153, and 169), suggesting general specificity for the basic PCB skeleton with varying affinities for different congeners. This aptamer provides a basis for constructing an affordable, sensitive, and high-throughput assay for the detection of PCBs in food and environmental samples and offers a promising alternative to existing methods of PCB quantitation. This study therefore advances aptamer technology by targeting one of the highly sought-after POPs, for the first time ever recorded. [less ▲]Detailed reference viewed: 42 (2 ULg)
Evaluation of the use of CALUX-results for dioxins and dioxin-like PCBs analysis for quantitative human exposure assessments
; ; et al
in Food Control (2012), 27(2), 314-321Detailed reference viewed: 38 (8 ULg)
Groundwater quality assessment of one former industrial site in Belgium using a TRIAD-like approach.
; Debacker, Virginie ; Joaquim-Justo, Célia et al
in Environmental Pollution (2011), 159(10), 2461-2466
Contaminated industrial sites are important sources of pollution and may result in ecotoxicological effects on terrestrial, aquatic and groundwater ecosystems. An effect-based approach to evaluate and ... [more ▼]
Contaminated industrial sites are important sources of pollution and may result in ecotoxicological effects on terrestrial, aquatic and groundwater ecosystems. An effect-based approach to evaluate and assess pollution-induced degradation due to contaminated groundwater was carried out in this study. The new concept, referred to as “Groundwater Quality TRIAD-like” (GwQT) approach, is adapted from classical TRIAD approaches. GwQT is based on measurements of chemical concentrations, laboratory toxicity tests and physico-chemical analyses. These components are combined in the GwQT using qualitative and quantitative (using zero to one subindices)integration approaches. The TRIAD approach is applied for the first time on groundwater from one former industrial site located in Belgium. This approach will allow the classification of sites into categories according to the degree of contaminant-induced degradation. This new concept is a starting point for groundwater characterization and is open for improvement and adjustment. [less ▲]Detailed reference viewed: 56 (18 ULg)
Determination and kinetics of enrofloxacin and ciprofloxacin in Tra catfish (Pangasianodon hypophthalmus) and giant freshwater prawn (Macrobrachium rosenbergii) using a liquid chromatography/mass spectrometry method
Danyi, Sophie ; Widart, Joëlle ; Douny, Caroline et al
in Journal of Veterinary Pharmacology & Therapeutics (2011), 34(2), 142-152Detailed reference viewed: 114 (39 ULg)
Optimisation of a new two-plate screening method for the detection of antibiotic residues in meat
; Degand, Guy ; Douny, Caroline et al
in International Journal of Food Science & Technology (2011), 46Detailed reference viewed: 55 (21 ULg)
OPTIMIZATION OF A NEW TWO-PLATE SCREENING METHOD FOR THE DETECTION OF ANTIBIOTIC RESIDUES IN MEAT.
; Degand, Guy ; et al
Poster (2011)Detailed reference viewed: 83 (4 ULg)
Modulation of CYP1A1 activity by a Ginkgo biloba extract in the human intestinal Caco-2 cells
; ; et al
in Toxicology Letters (2011), 202(3), 193-202Detailed reference viewed: 14 (3 ULg)
The application of reporter gene assays for the detection of endocrine disruptors in sport supplements
; ; Scippo, Marie-Louise et al
in Analytica Chimica Acta (2011), 700
The increasing availability and use of sports supplements is of concern as highlighted by a number of studies reporting endocrine disruptor contamination in such products. The health food supplement ... [more ▼]
The increasing availability and use of sports supplements is of concern as highlighted by a number of studies reporting endocrine disruptor contamination in such products. The health food supplement market, including sport supplements, is growing across the Developed World. Therefore, the need to ensure the quality and safety of sport supplements for the consumer is essential. <br />The development and validation of two reporter gene assays coupled with solid phase sample preparation enabling the detection of estrogenic and androgenic constituents in sport supplements is reported. Both assays were shown to be of high sensitivity with the estrogen and androgen reporter gene assays having an EC50 of 0.01 ng mL-1 and 0.16 ng mL-1 respectively. <br />The developed assays were applied in a survey of 63 sport supplements samples obtained across the Island of Ireland with an additional seven reference samples previously investigated using LC-MS/MS. Androgen and estrogen bio-activity was found in 71 % of the investigated samples. Bio-activity profiling was further broken down into agonists, partial agonists and antagonists. Supplements (13) with the strongest estrogenic bio-activity were chosen for further investigation. LC-MS/MS analysis of these samples determined the presence of phytoestrogens in seven of them. Supplements (38) with androgen bio-activity were also selected for further investigation. Androgen agonist bio-activity was detected in 12 supplements, antagonistic bio-activity was detected in 16 and partial antagonistic bio-activity was detected in 10. A further group of supplements (7) did not present androgenic bio-activity when tested alone but enhanced the androgenic agonist bio-activity of dihydrotestosterone when combined. <br />The developed assays offer advantages in detection of known, unknown and low-level mixtures of endocrine disruptors over existing analytical screening techniques. For the detection and identification of constituent hormonally active compounds the combination of biological and physio-chemical techniques is optimal. [less ▲]Detailed reference viewed: 114 (26 ULg)
Furan Formation from Lipids in Starch-Based Model Systems, As Influenced by Interactions with Antioxidants and Proteins.
; ; Scholl, Georges et al
in Journal of Agricultural and Food Chemistry (2011), 59(6), 2368-2376
The formation of furan upon sterilization of a lipid-containing starch gel was investigated in the presence of various antioxidants, namely, alpha-tocopherol, beta-carotene, and ascorbic acid, with and ... [more ▼]
The formation of furan upon sterilization of a lipid-containing starch gel was investigated in the presence of various antioxidants, namely, alpha-tocopherol, beta-carotene, and ascorbic acid, with and without proteins. Results indicated that alpha-tocopherol did not significantly influence furan formation from oxidized lipids. beta-Carotene, suggested previously to be a furan precursor itself, did influence the generation of furan in a concentration-dependent manner, although to a limited extent. Surprisingly, the presence of lipids seemed to limit the furan generation from beta-carotene. Interestingly, the addition of ascorbic acid to the emulsions containing soybean or sunflower oils considerably enhanced the formation of furan from these oils. This was also the case when fresh oils were applied, shown previously to be nearly unable to generate furan. This observation can be explained by an intensified ascorbic acid degradation stimulated by the presence of lipids. [less ▲]Detailed reference viewed: 51 (15 ULg)
Screening, identification and quantification of glucosinolates in black radish (Raphanus sativus L. niger) based dietary supplements using liquid chromatography coupled with a photodiode array and liquid chromatography mass spectrometry
; ; Scippo, Marie-Louise et al
in Journal of Chromatography. A (2011), 1218Detailed reference viewed: 65 (8 ULg)
Bisphenol A in our food : same toxicological studies but different risk assessment and risk management decisions around the world.
in Food Science and Law (2011), 5Detailed reference viewed: 21 (6 ULg)
“Development of an integrated strategy for controlling the allergen issue in the Belgian food and catering industry ALLERRISK”. Final Report. Brussels : Belgian Science Policy 2011 – 109 p. (Research Programme Science for a Sustainable Development)
; ; et al
Report (2011)Detailed reference viewed: 38 (1 ULg)