References of "Scippo, Marie-Louise"
     in
Bookmark and Share    
Full Text
Peer Reviewed
See detailModulation of CYP1A1 activity by a Ginkgo biloba extract in the human intestinal Caco-2 cells
Ribonnet, Laurence; Callebaut, Alfons; Nobels, Ingrid et al

in Toxicology Letters (2011), 202(3), 193-202

Detailed reference viewed: 13 (3 ULg)
See detailAntibiotics and pesticides residues in aquaculture products
Scippo, Marie-Louise ULg

Conference (2010, April 28)

Detailed reference viewed: 53 (13 ULg)
Full Text
Peer Reviewed
See detailREVISED EU-CRITERIA FOR APPLYING BIOANALYTICAL METHODS FOR SCREENING OF FEED AND FOOD FOR DIOXINS AND DIOXIN-LIKE PCBS.
Hoogenboom, LAP; Hadrich, Johan; Eppe, Gauthier ULg et al

in Organohalogen Compounds (2010), 72

Detailed reference viewed: 23 (1 ULg)
Full Text
Peer Reviewed
See detailRemoval of Androgens and Estrogens from Water by Reactive Materials
Kai, Cai; Phillips, D. H.; Elliott, Christian et al

in Journal of Water Resource and Protection (2010), 2(11), 990-993

Nowadays, endocrine disruptor compounds in the water system have become a concern due to the risk of contamination to wild life and humans even at the nanogram level. Excess estrogens and androgens are a ... [more ▼]

Nowadays, endocrine disruptor compounds in the water system have become a concern due to the risk of contamination to wild life and humans even at the nanogram level. Excess estrogens and androgens are a major contributor group of endocrine compounds. Statistical surveys have shown that dairy farms contribute to over 90% of the total estrogens in the UK and US. An analytical system is being developed to assess the efficiency of reactive materials to remove target hormonal contaminants from dairy farm effluent. This can be achieved using reporter gene assays (RGAs) to detect low level steroid hormones. A preliminary study comparing the efficiency of granular activated carbon, zero-valent iron, and organoclay was carried out using bench-scale evaluations in negative control HPLC water. Their potential ability to remove testosterone and 17-β- estradiol spiked at reported environmental levels was measured by androgenic and estrogenic luminescent reporter assay respectively. [less ▲]

Detailed reference viewed: 46 (10 ULg)
See detailSURVEY ON ANTIBIOTIC USE IN PIG AND CHICKEN PRODUCTION IN RED RIVER DELTA OF VIETNAM.
Pham Kim, Dang; Saegerman, Claude ULg; Vu Dinh, Ton et al

Poster (2010)

Detailed reference viewed: 16 (3 ULg)
Full Text
Peer Reviewed
See detailFuran formation from vitamin C in a starch-based model system: Influence of the reaction conditions
Owczarek-Fendor, Agnieska; De Meulenaer, Bruno; Scholl, Georges ULg et al

in Food Chemistry (2010), 121(4), 1163-1170

The generation of furan from vitamin C during thermal treatment of a starch-based model system, which simulated baby food, was studied. Results indicated that the amount of sample heated in the vial ... [more ▼]

The generation of furan from vitamin C during thermal treatment of a starch-based model system, which simulated baby food, was studied. Results indicated that the amount of sample heated in the vial influenced the furan generation from ascorbic acid. Increasing the amount of heated sample from 5% to approximately 98% of the total vial volume, drastically reduced furan formation from 70 ppb to 16 ppb. Changes in ascorbic acid concentrations from 0.1 to 4.5 mg/g did not influence furan concentration nor did different ascorbic/dehydroascorbic acid molar ratios. Interestingly, waxy corn starch itself considerably enhanced furan generation from ascorbic acid. Under the same conditions, 13.2 ppb of furan was generated in starch-based samples, while in ascorbic acid buffered solutions only 0.4 ppb of furan was formed. Application of other matrices, in particular agar and hydrolysed starch, resulted in similar furan concentrations as for native starch, while in polyol solutions furan concentrations were comparable to those obtained for the buffered ascorbic acid solutions. [less ▲]

Detailed reference viewed: 156 (54 ULg)
Full Text
Peer Reviewed
See detailAPPLICATION OF THE XDS-CALUX BIOASSAY IN ROUTINE: SEMI-QUANTITATIVE SCREENING USING AL-BEQ CUT-OFF VALUES
Vanderperren, H.; Haedrich, J.; Eppe, Gauthier ULg et al

in Organohalogen Compounds (2010)

Detailed reference viewed: 24 (1 ULg)
Full Text
Peer Reviewed
See detailDISCREPANCIES BETWEEN BIO-ANALYTICAL AND CHEMO-ANALYTICAL RESULTS HAVE A NON-NEGLIGIBLE MESSAGE
Goeyens, L.; Hoogenboom, R.; Eppe, Gauthier ULg et al

in Organohalogen Compounds (2010)

Detailed reference viewed: 15 (0 ULg)
See detailFuran formation in baby food model systems from vitamin C and unsaturated fatty acids
Owczarek-Fendor, Agnieszka; De Meulenaer, Bruno; Scholl, Georges ULg et al

Conference (2010)

Detailed reference viewed: 58 (17 ULg)
Full Text
Peer Reviewed
See detailImportance of fat oxidation in starch-based emulsions in the generation of the process contaminant furan.
Owczarek-Fendor, Agnieszka; De Meulenaer, Bruno; Scholl, Georges ULg et al

in Journal of Agricultural and Food Chemistry (2010), 58(17), 9579-86

The formation of the possibly carcinogenic process contaminant furan was studied in starch-based emulsions during heat treatments as applied for sterilization. Fresh and oxidized soybean, sunflower, high ... [more ▼]

The formation of the possibly carcinogenic process contaminant furan was studied in starch-based emulsions during heat treatments as applied for sterilization. Fresh and oxidized soybean, sunflower, high-oleic sunflower, olive, linseed, and rapeseed oils were compared. Results indicated that both the oil type, in particular, the fatty acid composition, and the oxidation degree of the oil determined the susceptibility of the oils to generate furan upon heating. Thus, oils containing the nutritionally relevant omega-3 unsaturated alpha-linolenic acid proved to be able to generate significant amounts of furan if the oils were oxidized. No clear relationship between p-anisidine values of various oils and the amount of generated furan could be observed. However, in the case of soybean oil, significantly more furan was produced upon an increase in oxidation degree. Surprisingly, furan formation in food-relevant systems containing fresh lipids proved to be a minor route (up to 1.5 ppb furan) compared to a previously studied vitamin C containing model system (up to 13 ppb furan). [less ▲]

Detailed reference viewed: 42 (12 ULg)
Full Text
Peer Reviewed
See detailValidation of a two-plate microbiological method for screening antibiotic residues in shrimp tissue
Pham Kim, Dang ULg; Degand, Guy ULg; Danyi, Sophie ULg et al

in Analytica Chimica Acta (2010), 672

Microbiological inhibition screening tests could play an important role to detect residues of antibiotics in the different animal food products, but very few are available for the aquaculture products in ... [more ▼]

Microbiological inhibition screening tests could play an important role to detect residues of antibiotics in the different animal food products, but very few are available for the aquaculture products in general, and for shrimps in particular. A two-plate microbiological method to screen shrimp for residues of the most commonly used antibiotics has been developed and validated according to criteria derived from the European Commission Decision 2002/657/CE. Bacillus subtilis was used as a sensitive strain to target antibiotics. Culture conditions on Petri plates (pH of medium) were selected to enhance the capacity of antibiotic detection. Antibiotic residues were extracted from shrimps using acetonitrile/acetone (70/30, v/v) before application on Petri plates seeded with B. subtilis. The method was validated using spiked blank tissues as well as antibiotic treated shrimps with enrofloxacin and tetracycline, two antibiotics often found to be used in shrimp production. For tetracyclines and (fluoro)quinolones, the detection capability was below the maximum residue limit (MRL), while it was around the MRL for sulfonamides. The specificity of the microbiological screening was 100% in all cases while the sensitivity and accuracy was 100% in almost all cases. The capacity of the method to detect contaminated samples was confirmed on antibiotic treated shrimps, analyzed in parallel with a confirmatory method (Liquid Chromatography coupled to mass spectrometry (LC–MS)). [less ▲]

Detailed reference viewed: 231 (22 ULg)
Full Text
Peer Reviewed
See detailUnderstanding the gap between the estrogenicity of an effluent and its real impact into the wild
Nadzialek, Stéphanie; Vanparys; Van Der Heiden, Edwige ULg et al

in Science of the Total Environment (2010), 408(4), 812-821

To study the reliability between in vitro and in vivo data collected downstream 2 sewage treatment plants (STP) as well as from bleached kraft mill industry (BKME), 5 rivers (3 impacted and 2 references ... [more ▼]

To study the reliability between in vitro and in vivo data collected downstream 2 sewage treatment plants (STP) as well as from bleached kraft mill industry (BKME), 5 rivers (3 impacted and 2 references) were investigated in the Walloon region (southern of Belgium). For the in vitro part of the work, water samples were collected to measure the estrogenicity of the ‘out’ effluent compared to reference sample point by MCF-7 assay. Results indicated significant estrogenicity of effluents from STP and BKME and a weak estrogenicity in reference sites. However, estradiol equivalents (EEQ) estimated into rivers were probably too low to impact wild population. Chemical analysis of 13 compounds of interest indicated that extraction procedure used in this study gave low recoveries of estrogen-like xenobiotics, leading to probably under-estimated MCF-7 responses. Surprisingly, a full scan mode has revealed an unexpected compound in the sample of BKME which was: 7-isopropyl-1,1,4a-trimethyl-1,2,3,4a,9,10,10a-octahydrophenanthrene, a product of pulp mill manufacture. In parallel to in vitro, in vivo assessment of estrogenic impact of effluent was followed on the gudgeon (Gobio gobio). Samples were achieved during 2 different periods of the reproductive cycle, resting period (RP) and pre-spawning period (pSP). Unspecific physiological parameters to estrogenic exposure (gonadosomatic index and systematic testis cell counting) displayed no significant differences related to endocrine disruption of the reproductive tract, only differences were correlated with the reproductive state of fish (RP versus pSP). Concerning the potent biomarker of estrogen exposure, vitellogenin (vtg), only basal induction was revealed but not related to estrogenic exposure. Nevertheless, vtg over-expression was found for male fish presenting a feminization of the reproductive tract captured downstream the STP station of Wégnez in the Vesdre River. Intersexuality, another indicator of the estrogenicity impact in fish, was observed in every site. Actually, ovotestis was systematically formed by protoplasmic oocyte observed in low percentage in every group analysed (impacted and references). Moreover, in fish captured in Wégnez, oocyte diameter was significantly higher compared to the other groups. In this study, only moderate to none impact in population of gudgeon was noticed. Moreover, in this case no discrepancy between in vitro and vivo was viewed although both approaches revealed gaps in monitoring effluent incidence into the environment. We should remain careful in the interpretation when only partial approaches are used in order to characterize impact in the aquatic milieu. [less ▲]

Detailed reference viewed: 36 (6 ULg)
Peer Reviewed
See detailRemoval of Androgens and Estrogens from Water by Reactive Materials
Kai, Cai; Phillips, D. H.; Elliott, Chris et al

in 2010 4th International Conference on Bioinformatics and Biomedical Engineering (iCBBE 2010) (2010)

Nowadays, endocrine disruptor compounds in the water system have become a concern due to the risk of contamination to wild life and humans even at the nanogram level. Excess estrogens and androgens are a ... [more ▼]

Nowadays, endocrine disruptor compounds in the water system have become a concern due to the risk of contamination to wild life and humans even at the nanogram level. Excess estrogens and androgens are a major contributor group of endocrine compounds. Statistical surveys have shown that dairy farms contribute to over 90% of the total estrogens in the UK and US. An analytical system is being developed to assess the efficiency of reactive materials to remove target hormonal contaminants from dairy farm effluent. This can be achieved using reporter gene assays (RGAs) to detect low level steroid hormones. A preliminary study comparing the efficiency of granular activated carbon, zero-valent iron, and organoclay was carried out using bench-scale evaluations in negative control HPLC water. Their potential ability to remove testosterone and 17- beta -estradiol spiked at reported environmental levels was measured by androgenic and estrogenic luminescent reporter assay respectively. [less ▲]

Detailed reference viewed: 50 (7 ULg)
Full Text
Peer Reviewed
See detailExpression characteristics of potential biomarker genes in Tra catfish, Pangasianodon hypophthalmus, exposed to trichlorfon
Sinha, Amit Kumar; Vanparys, Caroline; De Boeck et al

in Comparative Biochemistry and Physiology. Part D, Genomics & Proteomics (2010), 5(3), 207-216

Trichlorfon (TRC) is the most common organophosphorous insecticide used in aquaculture practices in Southeast Asian countries. Indiscriminate use of TRC can either damage or alter the enzymatic and ... [more ▼]

Trichlorfon (TRC) is the most common organophosphorous insecticide used in aquaculture practices in Southeast Asian countries. Indiscriminate use of TRC can either damage or alter the enzymatic and hormonal activities in the living organisms. In this present study, therefore, toxicogenomic analyses using real time PCR was used to characterize expression levels of various genes in Pangasianodon hypophthalmus after exposure to three concentrations, the 96 h 1/100LC50 (0.01 mg/L), the 96 h 1⁄10LC50 (0.1 mg/L) and the 96 h 1⁄2LC50 (0.5 mg/L) of TRC for 6 h, 24 h, 96 h, 7 days, 14 days, 28 days and 56 days respectively. The expression kinetics of stress and other cellular toxicity representative genes such as heat shock protein70 (HSP70), growth hormone, acetylcholinesterase (AChE), trypsinogen, cytochrome P4501B (CYP1B) and cytochrome oxidase subunit 1 (COI) were investigated in liver and gills. TRC at a level of 0.1 mg/L and 0.5 mg/L induced a time and dose-dependent increase in the expression of the HSP70, COI and CYPIB while the transcript level of AChE, growth hormone and trypsinogen were significantly down-regulated. These results could permit to develop a ‘molecular biomarker system’ which can be applied as a first-tier method of identifying contaminant exposure before effects at population level occur. [less ▲]

Detailed reference viewed: 113 (4 ULg)