References of "Scippo, Marie-Louise"
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See detailControl of the Illegal Administration of Natural Steroid Hormones in the Plasma of Bulls and Heifers
Scippo, Marie-Louise ULg; Degand, Guy ULg; Duyckaerts, Anne et al

in Analyst (1994), 119(12), 2639-44

In the context of the control of the illegal administration of natural steroid hormones in cattle husbandry, an attempt was made to establish the decision levels for sex steroid hormones in the plasma of ... [more ▼]

In the context of the control of the illegal administration of natural steroid hormones in cattle husbandry, an attempt was made to establish the decision levels for sex steroid hormones in the plasma of adult cattle, taking into account the effect of the treatment. Bulls and heifers were treated with two injections, at a two week interval, of an estradiol-testosterone cocktail. Steroid hormone and biochemical precursor concentrations were measured in plasma samples by using specific radioimmunoassays, before and after the treatment. When the treatment significantly (p < 0.05) modified a hormone concentration, a decision level was established for that hormone concentration. At each decision level, a score was assigned that represented the percentage of treated animals detected when the decision limit was applied. For heifers, 17 beta-estradiol and testosterone concentrations in plasma, which increased after the treatment, are the best criteria to use to detect treated animals, with decision limits of 20 pg ml-1 and 125 pg ml-1, respectively. In the instance of bulls, both testosterone and steroid biochemical precursor concentrations decreased in the plasma after the treatment. We proposed decision limits of 1500 pg ml-1 and 28 pg ml-1 for testosterone and androstenedione concentrations, respectively, the bulls displaying concentrations below these limits being positive. We observed that the repetition of the injection increased the score of the decision limit. The scores for testosterone are 70%, 14d after the first injection and 100% 14 d after the second injection, and for androstenedione, these scores are 60 and 100%, respectively. [less ▲]

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See detailDiscrimination of animals treated with growth promoters
Scippo, Marie-Louise ULg; Degand, Guy ULg; Gaspar, Pol et al

in Meat Focus International (1993)

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See detailPurification and Biochemical Characterization of Recombinant Human Placental Growth Hormone Produced in Escherichia Coli
Igout, Ahmed ULg; Van Beeumen, Jozef; Frankenne, Francis ULg et al

in Biochemical Journal (1993), 295(3), 719-724

The hGH-V (or hGH-2) gene codes for human placental growth hormone (hPGH). Secretion of hPGH is continuous, in contrast with the pulsed secretion of pituitary growth hormone (hGH) which it progressively ... [more ▼]

The hGH-V (or hGH-2) gene codes for human placental growth hormone (hPGH). Secretion of hPGH is continuous, in contrast with the pulsed secretion of pituitary growth hormone (hGH) which it progressively replaces in the maternal bloodstream. hGH- V cDNA has previously been cloned and isolated. Analysis of its nucleotide sequence has revealed a 191-residue protein, hPGH, differing from hGH at 13 positions. The calculated pI is more basic than that of the pituitary hormone. Here we have inserted hGH- VcDNA into the pIN-III-ompA3 plasmid in order to produce hPGH in its native form in Escherichia coli D1210. Expression of hGH- V cDNA in E. coli is significantly lower than that of hGH cDNA with the same expression system. The hPGH produced in E. coli was purified in quantities sufficient to allow its biochemical and immunochemical characterization. The molecular mass of the protein was determined by electrospray m.s. The determined mass, 22320 Da, agrees well with the molecular mass calculated from the translated cDNA sequence, assuming the presence of two disulphide bridges. Having established the technique for producing hPGH with a primary structure identical to the natural, non-glycosylated, 22 kDa isoform, we can now plan the full physicochemical and pharmaceutical characterization of this new hormonal entity. [less ▲]

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See detailSYNCYTIOTROPHOBLASTIC LOCALIZATION OF THE HUMAN GROWTH-HORMONE VARIANT MESSENGER-RNA IN THE PLACENTA
Scippo, Marie-Louise ULg; Frankenne, Francis ULg; HOOGHEPETERS, ELisabeth et al

in Molecular & Cellular Endocrinology (1993), 92(2), 7-13

The hGH/hCS genes, clustered on chromosome 17 in the 5' to 3' order GH-N, CS-L, CS-A, GH-V and CS-B, show a high degree of sequence identity. The expression product of the GH-V gene is the placental ... [more ▼]

The hGH/hCS genes, clustered on chromosome 17 in the 5' to 3' order GH-N, CS-L, CS-A, GH-V and CS-B, show a high degree of sequence identity. The expression product of the GH-V gene is the placental growth hormone, which replaces pituitary GH in maternal blood throughout pregnancy. By means of mRNA competitive hybridization using P-32-labelled and unlabelled 30 bases long oligonucleotides, we first optimized specific hybridization conditions. In situ hybridization was then performed to locate the GH-V mRNA encoding placental growth hormone. The hGH-V gene appears expressed in the placental syncytiotrophoblast. Unlike the CS-A and CS-B genes (both encoding hPL) which are expressed uniformly in the syncytiotrophoblast, the GH-V mRNA is located in a few syncytiotrophoblast cells only. [less ▲]

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See detailControl of the illegal administration of natural steroid hormones in urine and tissues of veal calves and in plasma of bulls
Scippo, Marie-Louise ULg; Gaspar, Pol; Degand, Guy ULg et al

in Analytica Chimica Acta (1993), 275(1-2), 57-74

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See detailEvaluation des méthodes de contrôle des anabolisants dans le cadre des décisions européennes
Willemart, Jean-Pierre; Scippo, Marie-Louise ULg; Gaspar, Pol et al

in Annales de Médecine Vétérinaire (1992), 136

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See detailEvidence for the expression of growth hormone receptors in human placenta
Frankenne, Francis; Alsat, Eliane; Scippo, Marie-Louise ULg et al

in Biochemical and Biophysical Research Communications (1992), 182(2), 481-486

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See detailHormone de croissance placentaire humaine : expression par génie génétique et relation structure-activité
Igout, Ahmed ULg; Frankenne, Francis; Scippo, Marie-Louise ULg et al

in Revue Française d'Endocrinologie Clinique, Nutrition, et Métabolisme (La) (1992), 3

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See detailHuman placental growth hormone
Frankenne, Francis; Evain-Brion, Danielle; Alsat, Eliane et al

in Anales Espanoles de Pediatria (1992), 36(50), 77-80

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See detailAdenohypophysis hormone gene products in 14 pituitary adenomas: analysis by immunohistochemistry and northern blotting.
Scippo, Marie-Louise ULg; Beckers, Albert ULg; Frankenne, Francis ULg et al

in Archives Internationales de Physiologie, de Biochimie et de Biophysique (1991), 99(2), 135-40

We investigated 14 pituitary adenomas (10 silent adenomas; 3 prolactinomas and one GH-secreting tumor) for the presence of hormone gene transcripts (Northern blot) as well as for translation products ... [more ▼]

We investigated 14 pituitary adenomas (10 silent adenomas; 3 prolactinomas and one GH-secreting tumor) for the presence of hormone gene transcripts (Northern blot) as well as for translation products (immunohistochemistry). The GH-secreting tumor was shown to express the genes coding for GH and PRL and to synthesize the corresponding hormones. In the cases of prolactinomas, immunohistochemical data demonstrated the synthesis of prolactin only. In addition to the PRL gene, Northern blot analysis revealed the transcription of the alpha-subunit gene in one case. Hormone genes were found to be expressed in 7 out of the 10 silent tumors, whereas no hormone synthesis was detected in any of these tissues. LH-beta mRNA was found in 3 cases, FSH-beta mRNA in 5 cases and alpha-subunit gene was shown to be expressed in one case. Surprisingly, the level of expression of the FSH-beta gene was higher than in normal tissue. This study confirms that some so called "silent" adenomas are expressing alpha- and/or beta-subunit glycoprotein hormone genes, even if no hormone is synthesized. The therapeutic action of bromocriptine described in some "silent" adenomas cases could be related to that hormone gene expression potentiality. [less ▲]

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See detailDemonstration of the pituitary expression of the human GH-V gene as assessed by a competitive hybridization technique.
Scippo, Marie-Louise ULg; Frankenne, F.; Igout, Ahmed ULg et al

in Proceedings of the 72nd Annual Meeting of the Endocrine Society (1990)

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See detailRegulation of growth hormone secretion in human trophoblastic cells in culture
Evain-Brion, Danielle; Alsat, Eliane; Mirlesse, Valérie et al

in Hormone Research (1990), 33(6), 256-259

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See detailIDENTIFICATION OF PLACENTAL HUMAN GROWTH-HORMONE AS THE GROWTH HORMONE-V GENE-EXPRESSION PRODUCT
Frankenne, Francis ULg; Scippo, Marie-Louise ULg; Van Beeumen, Jos et al

in Journal Of Clinical Endocrinology And Metabolism (1990), 71(1), 15-18

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See detailEndocrine, paracrine and autocrine factors in the maturation and functional development of the testis
Closset, Jean ULg; Dombrowicz, David; Vandenbroeck, Marc et al

in Bulletin et Mémoires de l'Académie Royale de Médecine de Belgique (1989), 144(1-2), 196-7

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See detailPITUITARY-HORMONES DEPENDENT EXPRESSION OF INSULIN-LIKE GROWTH FACTOR-I AND FACTOR-II IN THE IMMATURE HYPOPHYSECTOMIZED RAT TESTIS
Closset, Jean ULg; Gothot, André ULg; SENTE, Béatrice et al

in Molecular Endocrinology (1989), 3(7), 1125-1131

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See detailExpression and Secretion of the Human Placental Growth Hormone in Escherichia Coli
Igout, Ahmed ULg; Scippo, Marie-Louise ULg; Frankenne, Francis ULg et al

in Nucleic Acids Research (1989), 17(10), 3998

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See detailHuman placental growth hormone: proof of identity with the GH-V gene product by N-terminal microsequence analysis.
Scippo, Marie-Louise ULg; Frankenne, F.; Van Beumen, J. et al

in Archives Internationales de Physiologie et de Biochimie (1989), 97

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