Showing results 21 to 40 of 204
  Treatment of estrogens and androgens in dairy wastewater by a constructed wetland system.; ; et al in Water Research (2012), 46(7), 2333-43 Constructed wetland systems (CWS) have been used as a low cost bio-filtration system to treat farm wastewater. While studies have shown that CWS are efficient in removing organic compounds and pathogens ... [more ▼] Constructed wetland systems (CWS) have been used as a low cost bio-filtration system to treat farm wastewater. While studies have shown that CWS are efficient in removing organic compounds and pathogens, there is limited data on the presence of hormones in this type of treatment system. The objective of this study was to evaluate the ability of the CWS to reduce estrogenic and androgenic hormone concentration in dairy wastewater. This was achieved through a year long study on dairy wastewater samples obtained from a surface flow CWS. Analysis of hormonal levels was performed using a solid phase extraction (SPE) sample clean-up method, combined with reporter gene assays (RGAs) which incorporate relevant receptors capable of measuring total estrogenic or androgenic concentrations as low as 0.24 ng L(-1) and 6.9 ng L(-1) respectively. Monthly analysis showed a mean removal efficiency for estrogens of 95.2%, corresponding to an average residual concentration of 3.2 ng L(-1) 17beta-estradiol equivalent (EEQ), below the proposed lowest observable effect concentration (LOEC) of 10 ng L(-1). However, for one month a peak EEQ concentration of 115 ng L(-1) was only reduced to 18.8 ng L(-1). The mean androgenic activity peaked at 360 ng L(-1) and a removal efficiency of 92.1% left an average residual concentration of 32.3 ng L(-1) testosterone equivalent (TEQ). The results obtained demonstrate that this type of CWS is an efficient system for the treatment of hormones in dairy wastewater. However, additional design improvements may be required to further enhance removal efficiency of peak hormone concentrations. [less ▲] Detailed reference viewed: 14 (9 ULg)Groundwater quality assessment of one former industrial site in Belgium using a TRIAD-like approach.; Debacker, Virginie  ; Joaquim-Justo, Célia et alin Environmental Pollution (2011), 159(10), 2461-2466 Contaminated industrial sites are important sources of pollution and may result in ecotoxicological effects on terrestrial, aquatic and groundwater ecosystems. An effect-based approach to evaluate and ... [more ▼] Contaminated industrial sites are important sources of pollution and may result in ecotoxicological effects on terrestrial, aquatic and groundwater ecosystems. An effect-based approach to evaluate and assess pollution-induced degradation due to contaminated groundwater was carried out in this study. The new concept, referred to as “Groundwater Quality TRIAD-like” (GwQT) approach, is adapted from classical TRIAD approaches. GwQT is based on measurements of chemical concentrations, laboratory toxicity tests and physico-chemical analyses. These components are combined in the GwQT using qualitative and quantitative (using zero to one subindices)integration approaches. The TRIAD approach is applied for the first time on groundwater from one former industrial site located in Belgium. This approach will allow the classification of sites into categories according to the degree of contaminant-induced degradation. This new concept is a starting point for groundwater characterization and is open for improvement and adjustment. [less ▲] Detailed reference viewed: 33 (8 ULg)The application of reporter gene assays for the detection of endocrine disruptors in sport supplements; ; Scippo, Marie-Louise et alin Analytica Chimica Acta (2011), 700 The increasing availability and use of sports supplements is of concern as highlighted by a number of studies reporting endocrine disruptor contamination in such products. The health food supplement ... [more ▼] The increasing availability and use of sports supplements is of concern as highlighted by a number of studies reporting endocrine disruptor contamination in such products. The health food supplement market, including sport supplements, is growing across the Developed World. Therefore, the need to ensure the quality and safety of sport supplements for the consumer is essential. <br />The development and validation of two reporter gene assays coupled with solid phase sample preparation enabling the detection of estrogenic and androgenic constituents in sport supplements is reported. Both assays were shown to be of high sensitivity with the estrogen and androgen reporter gene assays having an EC50 of 0.01 ng mL-1 and 0.16 ng mL-1 respectively. <br />The developed assays were applied in a survey of 63 sport supplements samples obtained across the Island of Ireland with an additional seven reference samples previously investigated using LC-MS/MS. Androgen and estrogen bio-activity was found in 71 % of the investigated samples. Bio-activity profiling was further broken down into agonists, partial agonists and antagonists. Supplements (13) with the strongest estrogenic bio-activity were chosen for further investigation. LC-MS/MS analysis of these samples determined the presence of phytoestrogens in seven of them. Supplements (38) with androgen bio-activity were also selected for further investigation. Androgen agonist bio-activity was detected in 12 supplements, antagonistic bio-activity was detected in 16 and partial antagonistic bio-activity was detected in 10. A further group of supplements (7) did not present androgenic bio-activity when tested alone but enhanced the androgenic agonist bio-activity of dihydrotestosterone when combined. <br />The developed assays offer advantages in detection of known, unknown and low-level mixtures of endocrine disruptors over existing analytical screening techniques. For the detection and identification of constituent hormonally active compounds the combination of biological and physio-chemical techniques is optimal. [less ▲] Detailed reference viewed: 61 (24 ULg)Screening, identification and quantification of glucosinolates in black radish (Raphanus sativus L. niger) based dietary supplements using liquid chromatography coupled with a photodiode array and liquid chromatography mass spectrometry; ; Scippo, Marie-Louise et alin Journal of Chromatography. A (2011), 1218 Detailed reference viewed: 36 (8 ULg) Bisphenol A in our food : same toxicological studies but different risk assessment and risk management decisions around the world.Scippo, Marie-Louise in Food Science and Law (2011), 5 Detailed reference viewed: 10 (1 ULg)“Development of an integrated strategy for controlling the allergen issue in the Belgian food and catering industry ALLERRISK”. Final Report. Brussels : Belgian Science Policy 2011 – 109 p. (Research Programme Science for a Sustainable Development); ; et al Report (2011) Detailed reference viewed: 22 (1 ULg)Food interactions : effects on health, consumer perception and impact on agro-food industries “FOODINTER”.Scippo, Marie-Louise ; ; et alReport (2011) Detailed reference viewed: 30 (9 ULg)Furan formation in baby food model system via lipid oxidation and sugar degradation; ; Scholl, Georges et alin Communications in Agricultural and Applied Biological Sciences (2011), 76(1), 107-110 Detailed reference viewed: 21 (4 ULg)Development of a LC-UV-MS analytical method for malondialdehyde.Douny, Caroline ; ; Brose, François et alPoster (2011) Detailed reference viewed: 6 (2 ULg)OPTIMIZATION OF A NEW TWO-PLATE SCREENING METHOD FOR THE DETECTION OF ANTIBIOTIC RESIDUES IN MEAT.; Degand, Guy ; et alPoster (2011) Detailed reference viewed: 10 (1 ULg)DEVELOPMENT OF A LIQUID CHROMATOGRAPHY/TANDEM MASS SPECTROMETRY METHOD TO DETECT AND COMPARE KINETICS OF ENROFLOXACIN AND CIPROFLOXACIN ACCUMULATION AND DECONTAMINATION IN TRA CATFISH AND SHRIMP.Scippo, Marie-Louise ; ; Widart, Joëlle et alPoster (2011) Detailed reference viewed: 12 (2 ULg)DETERMINATION AND KINETICS OF MALACHITE GREEN AND LEUCOMALACHITE GREEN IN TRA CATFISH (PANGASIONODON HYPOPHTHALMUS).Scippo, Marie-Louise ; ; Baiwir, Dominique et alConference (2011) Detailed reference viewed: 5 (1 ULg)DETERMINATION OF TRIFLURALIN RESIDUES IN CULTURED CATFISH AND WATER.; ; et al Poster (2011) Detailed reference viewed: 16 (3 ULg)Mise au point d'une stratégie analytique pour le contrôle des résidus d'acide oxolinique dans la chair du Tilapia "Oreochromis niloticus". Scientific Day of the Faculty of Veterinary Medicine, Liège, 9 December 2011.; ; Douny, Caroline et alPoster (2011) Detailed reference viewed: 12 (3 ULg)Development of a LC-UV-MS analytical method for aldehydes formed during fatty acids degradation.Douny, Caroline ; ; Brose, François et alPoster (2011) Detailed reference viewed: 12 (2 ULg)The Use of Phages and Aptamers as Alternatives to Antibodies in Medical and Food Diagnostics.; ; et al in Komorowska, Malgorzata Anna; Olsztynska-Janus, Sylwia (Eds.) Biomedical Engineering, Trends, Research and Technologies (2011) Detailed reference viewed: 49 (5 ULg)Faut-il interdire le Bisphenol A dans les récipients destinés à contenir des aliments ?Scippo, Marie-Louise in Culture, le magazine culturel en ligne de l'Université de Liège - DOSSIER/ Controverses scientifiques - http://culture.ulg.ac.be/jcms/prod_494506/dossier/-controverses-scientifiques?section=cdu_5047 (2011) Detailed reference viewed: 33 (4 ULg)Potential of an in vitro toolbox combined with exposure data as a first step for the risk assessment of dietary chemical contaminants; Van Der Heiden, Edwige ; et alin Food Additives & Contaminants (2011), 28(9), 1136-1158 In vitro risk assessment of dietary contaminants has become a priority in human food safety. This paper proposes an in vitro approach associating different complementary tools in an original toolbox and ... [more ▼] In vitro risk assessment of dietary contaminants has become a priority in human food safety. This paper proposes an in vitro approach associating different complementary tools in an original toolbox and aims to improve the assessment of the toxicological impact of dietary contaminants at realistic human exposure levels, with a special focus on the intestinal compartment. The system is based on the use of four complementary cellular tools, namely stress gene induction in transgenic strains of Escherichia coli, modulation of the activity of key biotransformation enzymes (cytochrome P-450 (CYP) 1A1 and 3A4) in a human intestinal cell line, and activation of aryl hydrocarbon receptor (AhR) and oestrogenic receptor (ER)-dependent genes in agonistic and antagonistic assays with luciferase reporter cells. It was applied to four chosen model molecules: ochratoxin A (OTA) and deoxynivalenol (DON), two common food-borne mycotoxins, and imazalil (IMA) and benomyl (BEN), two fungicides widely occurring in foodstuffs. All these assays were performed at or around a realistic intestinal concentration, determined through a deterministic approach based on the calculation of a theoretical maximum daily intake (TMDI). Using the four model molecules, it is clearly highlighted that induction of CYP1A1 activity and inhibition of CYP3A4 activity occurred in Caco-2 cells at a realistic intestinal concentration of IMA. Furthermore, some bacterial stress genes were induced in a range of realistic concentrations, following exposure to DON and IMA. In addition, BEN clearly provoked an ER agonistic activity in a human oestrogen sensitive reporter cell line. All these results are in accordance with the literature, suggesting that the in vitro toolbox constitutes an interesting approach in order to obtain a first 'fingerprint' of dietary contaminants at realistic human exposure for further risk assessment. [less ▲] Detailed reference viewed: 10 (0 ULg)Optimisation of a new two-plate screening method for the detection of antibiotic residues in meat; Degand, Guy ; Douny, Caroline et alin International Journal of Food Science & Technology (2011), 46 Detailed reference viewed: 34 (12 ULg)New approach to quantitative analysis of benzo[a]pyrene in food supplements by an immunochemical column test; ; et al in Talanta (2011), 85 Detailed reference viewed: 24 (9 ULg) |
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