References of "Scippo, Marie-Louise"
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See detailInfluence of aging technique and muscle on physicochemical stability and antioxidant capacity of high-oxygen atmosphere packed beef
Didimo Imazaki, Pedro Henrique ULg; Tahiri, Assia ULg; Thimister, Jacqueline ULg et al

Poster (2014, August)

The aim of this study was to compare the effect of aging technique (wet-aging vs. carcass-aging), muscle (longissimus dorsi vs. rectus femoris) and previous vacuum storage time on color and lipid ... [more ▼]

The aim of this study was to compare the effect of aging technique (wet-aging vs. carcass-aging), muscle (longissimus dorsi vs. rectus femoris) and previous vacuum storage time on color and lipid stability of beef packaged in high-oxygen atmosphere. After a seven-day wet- or carcass-aging step, longissimus dorsi and rectus femoris muscle cuts from 4 Belgian Blue cows were vacuum packaged and stored at −1 °C for up to 28 days. At different times, part of these samples was repackaged under modified atmosphere – 70 % O2:30 % CO2 –, and stored during 7 days at +4 °C. The following parameters were evaluated at different intervals: color (CIE L*a*b*), metmyoglobin %, lipid oxidation (TBARS), antioxidant enzyme activities (catalase, glutathione peroxidase and superoxide dismutase), alpha-tocopherol and fat content. The sensitivity of modified atmosphere repacked meat cuts to oxidation was influenced by the conditions of the previous aging period (wet > carcass conditions), muscle (rectus femoris > longissimus dorsi) and length of the vacuum storage. Oxidation stability could be associated with catalase activity, and no association could be established with the alpha-tocopherol content. [less ▲]

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See detailProduction and microbiological evaluation of three types of "Dèguè", a local fermented drink made from milk in Benin
Tchekessi, Célestin; Bokossa, Auréole; Agbangla, Clément et al

in International Journal of Multidisciplinary and Current Research (2014), 2

This study consists to finalize some technologies for the production of a fermented drink called dèguè. This drink is widely consumed in Benin and other countries in sub-Saharan Africa. Following three ... [more ▼]

This study consists to finalize some technologies for the production of a fermented drink called dèguè. This drink is widely consumed in Benin and other countries in sub-Saharan Africa. Following three different technologies, we had produced three (03) types of dèguè respectively with maize flour, sorghum and millet. These types have been analyzed and their microbiological characteristics were evaluated. The microbiological analysis results obtained from the experiments have shown that lactic acid bacteria, yeasts and molds were the dominant microflora of dèguè and varied respectively 7.22log10UFC/g to 7.55log10UFC/g for lactic acid bacteria and 7.78log10UFC/g to 8.44log10UFC/g for yeasts and molds. Moreover, the statistical analysis of these results showed that there was no significant difference at 5% (p <0.05) between the three types of dèguè. The values of lactic acid bacteria obtained were consistent with the standard (≥ 107/g) attached to the yoghurt. No type contained neither total coliforms nor thermotolerant coliforms. The products (dèguès) were also free of pathogenic microorganisms such as Escherichia coli, Staphylococcus aureus and Salmonella. [less ▲]

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See detailIn vitro model to study the endocrine disrupting activity of migration products from plastic food contact materials
Simon, Coraline ULg; Onghena, Matthias; Covaci, Adrian et al

Poster (2014, May 15)

Bisphenol A (BPA) is used since 1960 as a primary raw material for the production of polycarbonate (PC) plastic and epoxy resin, which are widely used in a variety of common products including digital ... [more ▼]

Bisphenol A (BPA) is used since 1960 as a primary raw material for the production of polycarbonate (PC) plastic and epoxy resin, which are widely used in a variety of common products including digital media (e.g., CDs, DVDs), electrical and electronic equipment, automobiles, sports safety equipment, reusable food and drink containers , as well as baby bottle. During the last decades, in several studies, the migration of BPA is documented to be a well-known source of food contamination. The measurements of BPA in human fluids and tissues highlighted that its presence in food constitutes the primary route of human exposure. Some studies showed that BPA, which could disrupt normal endocrine function by mimicking estrogen hormones,, may be associated to several health problems and diseases. Recently, the European food safety authority conducted a risk assessment on BPA and concluded that though studies related to potential health hazards associated with BPA, are suggesting a potential negative effect on human body, but results are still uncertain. Following that screening assessment, the European Union took a series of measures, including a ban for the manufacture, import and sale of PC baby bottles to reduce BPA exposure of infants. Plastic alternatives to polycarbonate have massively appeared on Belgium market. Although there are several studies on BPA migration from polycarbonate into foodstuff under various conditions, there is a small amount of information about consequences on human health of the chemicals migrating from PC alternatives, including bottles commonly labelled “free BPA”. In a recent opinion (No. 8697, 11.03.2010), the Belgium Superior Health Council's issued its concern regarding the currently used alternatives to PC. Furthermore, they asked to investigate the possible risks associated with the use of these alternatives. To know if these alternatives are safe, the activity on several receptors (estrogen (ER), androgen (AR), progesterone (PR) and glucocorticoïd receptor (GR)) of chemicals migrating from PC alternatives, identified by Simoneau & al, 2012 , were evaluated using reporter gene assays. Agonistic and antagonistic activities of 38 pure compounds were measured. After the first screening, some substances clearly showed an activity on each receptor, such as BPA, 2.4- dimethyl benzaldehyde (C4), Bisphenol S (C49), while other subtances reacted on three, two or one receptor. Only 5 substances showed no activity. For active substances only, the study will be continued and a full dose-response curve will be performed in order to assess quantitatively the activity. [less ▲]

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See detailEffect of fuel and kiln type on the polycyclic aromatic hydrocarbon (PAH) levels in smoked shrimp, a Beninese food condiment
Kpoclou, Euloge; Anihouvi, Victor; Azokpota, Paulin et al

in Food Additives & Contaminants. Part A. Chemistry, Analysis, Control, Exposure & Risk Assessment (2014), 31(7), 1212-1218

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See detailUSING TOP PREDATORS AND DR-CALUX TO SCREEN COASTAL ENVIRONMENTS FROM THREE DIFFERENT BRAZILIAN REGIONS FOR DIOXINS AND RELATED COMPOUNDS
Dorneles; Lailson-Brito; Bisi et al

in Organohalogen Compounds (2014), 76

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See detailSteroid-like activity of migration products from non polycarbonate plastic baby bottles.
Simon, Coraline ULg; Onghena, Matthias; Covaci, Adrian et al

in Toxicology Letters (2014), 229

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See detailIn vitro study toward the endocrine activity and the genotoxic potential of migration products from plastic baby bottles
Simon, Coraline ULg; Onghena, M.; Covaci, A. et al

in Organohalogen Compounds (2014), 76

Bisphenol A (BPA) is documented in several studies to be a well-known source of food contamination. Recently, the European Union took a series of measures, including a ban for the import and sale of ... [more ▼]

Bisphenol A (BPA) is documented in several studies to be a well-known source of food contamination. Recently, the European Union took a series of measures, including a ban for the import and sale of polycarbonate (PC) baby bottles to reduce BPA exposure of infants. Plastic alternatives to PC, which have massively appeared on the Belgian market, include polypropylene (PP), silicone, polyamide (PA) and polyethersulfone (PES). In a recent opinion (No. 8697, 11.03.2010), the Belgian Superior Health Council issued its concern regarding the alternatives to PC currently used. Furthermore, they asked to investigate the possible risks associated with the use of these alternatives. In this study, a screening towards the endocrine activity of chemicals migrating from PC alternatives, identified by the group of Simoneau, was performed by using different reporter gene assays. Furthermore, the genotoxic potential of these compounds was also assessed with the Vitotox assay, an indicator test for DNA damage. The aim of the screening was to select the substances that may present a risk for human health and thus require further characterization. [less ▲]

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See detailEDCs & Plastic baby bottles
Simon, Coraline ULg; Scippo, Marie-Louise ULg

Conference (2014)

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See detailEffect of land use on pollution status and risk of fish endocrine disruption in small farmland ponds
Mandiki; Gillardin; Martens et al

in Hydrobiologia (2014), 723

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See detailIsolation and Quantification of Lactic Acid Bacteria from Traditional Fermented Products in Benin
Tchekessi, C.K.C.; Bokossa, I.Y; Azokpota, P. et al

in International Journal of Current Microbiology and Applied Sciences (2014), 3(11), 1-8

In Africa, fermented food products are particularly used as weaning foods for young children, pregnant women and the seniors. In Benin, most of these cereals-based foods are manufactured and sold around ... [more ▼]

In Africa, fermented food products are particularly used as weaning foods for young children, pregnant women and the seniors. In Benin, most of these cereals-based foods are manufactured and sold around the streets. These are ablo, dèguè, akpan, abotin, gowé etc ... This study focused on the isolation and enumeration of bacteria and yeast from twenty six (26) samples of traditional African fermented foods. Decimal dilution method allowed us to isolate a total of 42 different strains of microorganisms among which we counted 30 lactic acid bacteria. They confirmed their biological potential by expanding in selective medium MRS. When they were then subjected to screening, the medium of MRS-broth-starch agar, and eight (08) of the isolated strains tested showed their characters of amylolytic strains. The eight strains isolated were tested and finally cultivated at temperatures and pH ranging respectively from 30°C to 60°C and 3.0 to 6.5. These eight (08) amylolytic lactic acid bacteria (ALAB) synthesized enzyme amylase both inside and outside the bacterial cell with a very interesting activity value. The temperature limit between 40 and 45°C is best for the bacteria 26.2, B16 and B18 while the optimum pH for amylase synthesis is between 4.0 and 4.5 for the same microorganisms that have given the largest values of enzyme activity. The best strains 26.2, B16 and B18 secrete more enzymes in extracellular medium than intracellular. [less ▲]

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See detailDioxins and polychlorinated biphenyls contamination in poultry liver related to food safety - A review
Ghimpeteanu, Oana-Margarita; Militaru, Manuella; Scippo, Marie-Louise ULg

in Food Control (2014), 38

The present article reviews the most important scientific literature on dioxins and PCBs found in poultry liver and their relation with food safety and consumers’ health. Dioxins and polychlorinated ... [more ▼]

The present article reviews the most important scientific literature on dioxins and PCBs found in poultry liver and their relation with food safety and consumers’ health. Dioxins and polychlorinated biphenyls (PCBs) are persistent organic pollutants with high chemical stability; they are lipophilic compounds and they are not destroyed by microbial, photochemical, chemical or thermal degradation. Dioxins and PCBs are ubiquitous environmental contaminants, which are present in all marine plant and animals, birds, mammals and humans and bioaccumulate through the food chain. In the last years, there has been concern regarding food contamination with different chemical substances and their effect on food safety. More particularly, at the end of the 20th and the beginning of the 21st century, there were a series of incidents related to dioxin and PCBs, which directly affected human or contaminated the soil and accumulated in feed and then in food products, such as poultry liver. It was shown in case of dioxin incident that tetra and penta-chlorinated congeners (the most toxic ones) accumulates selectively in poultry livers. Maximal concentrations have been fixed in the European legislation for dioxins and PCBs in food from animal origin, in order to protect the consumer. Data about background poultry liver contamination are scarce and the few available show levels below the legal limit for dioxins, but data are still lacking for DL-PCBs. [less ▲]

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See detailIn vitro model to study the endocrine disrupting activity of migration products from plastic food contact materials
Simon, Coraline ULg; Onghena, Matthias; Covaci, Adrian et al

Poster (2013, December 06)

Bisphenol A (BPA) is a chemical compound mainly used for the manufacture of plastic such as polycarbonate. This transparent thermoplastic polymer is used for the fabrication of several food containers ... [more ▼]

Bisphenol A (BPA) is a chemical compound mainly used for the manufacture of plastic such as polycarbonate. This transparent thermoplastic polymer is used for the fabrication of several food containers like baby bottle, cups, etc. BPA can migrate into food in contact with polycarbonate. There is a worldwide concern about BPA because several studies have shown endocrine disruptor potency of BPA causing possible adverse health effects. In January 2011, the European Commission decided to ban the use of polycarbonate to manufacture baby feeding bottles. In a recent opinion, the Superior Health Council’s issued its concern regarding the currently use of alternatives to polycarbonate in these materials. This work is part of the ALTPOLYCARB project, which aims to study the migration products from non polycarbonate plastic material, and their endocrine disruptor activities. The endocrine disruptor activity(ies) of global migration residues obtained from different kinds of baby bottles will be explored using cell based transactivational assays also named “reporter gene assays”. The MCF7 recombinant cells used here (named MCF7-ER cells) are genetically modified cells containing the firefly luciferase gene, as a reporter gene, and a DNA responsive element specific to the human estrogen receptor. The biological activity of a chemical compound is monitored by the measurement of light emitted by the cells exposed to it (after addition of luciferin, the substrate of luciferase). In a preliminary step, we first screened pure substances, which were shown to migrate from plastic baby bottles, in a recent study performed by Simoneau & al, 2012 . Human estrogen receptor agonistic and antagonistic activities of 25 pure compounds were measured using MCF7-ER cells. After the first screening, some substances clearly show an activity such as BPA, benzophenone, 2-propenoic acid-2-ethylhexyl ester, benzaldehyde-4-methylthio, butylated hydroxytoluene and dodecanoic acid methyl ester, whereas others ask an in-depth analysis to confirm their activity. For active substances only, the study will be continued and a full dose-response curve will be performed in order to assess quantitatively the activity. [less ▲]

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See detailMicrobial, biochemical and sensorial quality assessment of Algerian farmed tilapia (Oreochromis niloticus) stored at 4 and 30°C
Dergal, Nadir; Abi-Ayad, S.M.E.-A.; Degand, Guy ULg et al

in African Journal of Food Science (2013), 7(12), 498-507

Detailed reference viewed: 63 (18 ULg)
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See detailIn vitro model to study the endocrine disrupting activity of migration products from plastic food contact materials
Simon, Coraline ULg; Onghena, Matthias; Covaci, Adrian et al

Poster (2013, November 06)

Bisphenol A (BPA) is a chemical compound mainly used for the manufacture of plastic such as polycarbonate. This transparent thermoplastic polymer is used for the fabrication of several food containers ... [more ▼]

Bisphenol A (BPA) is a chemical compound mainly used for the manufacture of plastic such as polycarbonate. This transparent thermoplastic polymer is used for the fabrication of several food containers like baby bottle … BPA can migrate into food in contact with polycarbonate. There is a worldwide concern about BPA because several studies have shown endocrine disruptor potency of BPA causing possible adverse health effects. In January 2011, the European Commission decided to ban the use of polycarbonate to manufacture baby feeding bottles. In a recent opinion, the Superior Health Council’s issued its concern regarding the currently use of alternatives to polycarbonate in these materials. This work is part of the ALTPOLYCARB project which aims to study the migration products of alternative to polycarbonate and their endocrine disruptor activities. The first part was to have an overview of the different polymers replacing polycarbonate, that are used on the Belgian market, it resulted in the conclusion that polymers used for the manufacture of baby bottles are mainly polypropylene, polyethersulfone, silicone, polyamide, polystyrene, and melamine. The second part of this work will be to evaluate the endocrine disruptor activity(ies) of global migration residues obtained from different kinds of baby bottles. This (these) activity(ies) will be explored using cell based transactivational assays also named “reporter gene assays. The MCF7 recombinant cells used here are genitically modified cells containing the firefly luciferase gene, as a reporter gene, and a DNA responsive element specific to the human oestrogen receptor. The biological activity of a chemical compound is monitored by the measurement of light emitted by the cells exposed to it (after addition of luciferin, the substrate of luciferase). In a preliminary step, we first screened pure substances which were shown to migrate from plastic baby bottle, in a recent study performed by Simoneau & al, 2012 . Human estrogen receptor agonistic and antagonistic activities of 25 pure compounds were measured using MCF7-ER cells (genetically modified MCF7 cells). After the first screening, some substances clearly show an activity such as BPA, Benzophenone, 2-Propenoic acid-2-ethylhexyl ester, Benzaldehyde-4-methylthio, Butylated hydroxytoluene and Dodecanoic acid, methyl ester whereas others ask an in-depth analysis to confirm their activity. For active substances only the study will be continued and a full dose-response curve will be performed in order to assess quantitatively the activity [less ▲]

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See detailMeasurement of the estrogenic activity of migration products from plastic contact materials
Simon, Coraline ULg; Onghena, M.; Covaci, A. et al

Poster (2013, October 19)

Baby bottles in plastic are widely used to feed newborns and infants. The polymers used for the manufacture of baby bottles are mainly polypropylene (PP), silicone, polyamide (PA) and polyethersulphone ... [more ▼]

Baby bottles in plastic are widely used to feed newborns and infants. The polymers used for the manufacture of baby bottles are mainly polypropylene (PP), silicone, polyamide (PA) and polyethersulphone (PES), since the ban of the use of bisphenol A (BPA), which exhibited estrogenic activity, in polycarbonate by European commission, in March 2011. For the plastic substitutes, Chemical substances, such as monomers or additives (antioxidants, plasticizers, clarifiers, etc.), may migrate from plastic baby bottle into its contents. This monomers and additives may exhibit at low concentrations estrogenic activity (EA), who can produce adverse effects, especially in newborn and infants. A recent study of Simoneau and al. give an overview of the amount of chemical substances which migrate from plastic baby bottle into its contents. The objective of this study is to measure EA of these substances. The study focus first on screening Human estrogen receptor agonistic and antagonistic activities of pure compounds identifed by Simoneau and al. using a cell based transactivational assay named “Chemically Activated LUciferase gene eXpression” (CALUX). Preliminary test with 3 Bisphenols (BPs) have been performed: BPA, Bisphenol S (BPS) and Bisphenol F (BPF). The dose–response curves obtained for BPA, BPS and BPF were compared with that of 17β-estradiol (E2). An agonistic effect was observed with the 3 BPs. BPS and BPF exhibited oestrogen-like response lower than that of BPA. [less ▲]

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See detailStudy of the estrogenicity of pure compound migrate from plastic food contact materials
Simon, Coraline ULg; Oghena, M.; Covaci, A. et al

Poster (2013, September 19)

Detailed reference viewed: 33 (2 ULg)