Persistent organochlorine pollutants, dioxins and polychlorinated biphenyls

in Contaminant and Residue Analysis, Comprehensive Analytical Chemistry of Elsevier (2008)

Cell based assay dioxin screening: utilisation of three new cellular bioassays from different species and tissues

in Organohalogen Compounds (2008), 70

Development and validation of a HPLC/UV/FLD method to detect the 15 (+1) eu priority PAHs in smoke flavourings

Conference (2007, June)

Validation of the analytical procedure for the determination of polyaromatic hydrocarbons in smoke flavourings using high performance liquid chromatography coupled to an ultraviolet, diode array or fluorescence detector

in Accreditation and Quality Assurance (2007), 12(10), 535-542

High performance liquid chromatography (HPLC) coupled to an ultraviolet (UV), diode array or fluorescence detector (UV/DAD/FLD) has been used to set up an analytical procedure for the quantification of 16 ... [more ▼]

High performance liquid chromatography (HPLC) coupled to an ultraviolet (UV), diode array or fluorescence detector (UV/DAD/FLD) has been used to set up an analytical procedure for the quantification of 16 EU priority polyaromatic hydrocarbons (PAHs) in smoke flavourings. The following parameters have been determined for the 16 EU priority PAHs: limit of detection, limit of quantification, precision (repeatability and intermediate precision), recovery and measurement uncertainty, using the concept of accuracy profiles. They were in close agreement with quality criteria described in the Commission Regulation (EC) no. 627/2006 concerning PAHs in smoke flavourings. [less ▲]

Analysis of furan by GC/MS

Poster (2007)

ARYL HYDROCARBON RECEPTOR-MEDIATED AGONIST/ANTAGONIST/SYNERGIC ACTIVITIES OF FOOD POLYPHENOLS ARE SPECIES- AND TISSUE-DEPENDENT

in Organohalogen Compounds (2007), 69

Solubilisation and binding characteristics of a recombinant beta(2)-adrenergic receptor expressed in the membrane of Escherichia coli for the multianalyte detection of beta-agonists and antagonists residues in food-producing animals

in Analytica Chimica Acta (2007), 589(2), 159-165

The number of substances with beta-agonistic activity, illegally introduced in meat production or in sports doping as anabolic or beta-blocking agents is increasing. Analytical methods suited for their ... [more ▼]

The number of substances with beta-agonistic activity, illegally introduced in meat production or in sports doping as anabolic or beta-blocking agents is increasing. Analytical methods suited for their multianalyte detection are thus necessary. In this perspective, receptor assays were developed. The research activities undertaken in this study describe the solubilisation of a recombinant human beta(2)-adrenergic receptor produced in the inner membrane of genetically modified Escherichia coli, using the detergent n-dodecyl-beta-D-maltoside. Its potential to detect the presence of beta-agonists or beta-blockers in biological samples was evaluated. The solubilised beta(2)-adrenergic receptor retained its binding affinity in a radio-receptor assay based on the competition for the binding to receptors between a ligand (beta-agonist or antagonist) and the radioligand [I-125]iodocyanopindolol. The IC50 values ranged from 5 +/- x 10(-8) M (clenbuterol) to 8 +/- 2 x 10(-6) M (isoxsuprine) for the beta-agonists tested and from 1.5 +/- 0.2 x 10(-10) M (carazolol) to 1.2 +/- 0.2 x 10(-5) M (metoprolol) for the beta-blockers tested. It was shown to have a lower limit of detection than a radio-receptor assay using the solubilised beta(2)-adrenoceptor expressed in a mammalian cell line. The solubilised recombinant human beta(2)-adrenoreceptor expressed in E. coli would be a useful tool to develop non radioactive multianalyte screening methods. (c) 2007 Elsevier B.V. All rights reserved. [less ▲]

Imazalil is an aryl hydrocarbon receptor (AhR) antagonist in AhR-dependent reporter human and rat hepatoma cells

in Toxicology Letters (2007), 172

Study of the 16 EU-JECFA PAHs interaction with the aryl hydrocarbon receptor (AhR) using rat and human reporter cell lines

in Toxicology Letters (2007), 172

New experimental approach of identification and characterization of emerging risks in food safety (EMRISK).

Report (2007)