References of "Scippo, Marie-Louise"
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See detailLC-MS/MS multi-analyte method for mycotoxin determination in food supplements
Di Mavungu, Jose Diana; Monbaliu, Sofie; Scippo, Marie-Louise ULg et al

in Food Additives & Contaminants (2009), 26(6), 885-895

A multi-analyte method for the liquid chromatography-tandem mass spectrometric determination of mycotoxins in food supplements is presented. The analytes included A and B trichothecenes (nivalenol ... [more ▼]

A multi-analyte method for the liquid chromatography-tandem mass spectrometric determination of mycotoxins in food supplements is presented. The analytes included A and B trichothecenes (nivalenol, deoxynivalenol, 3-acetyldeoxynivalenol, 15-acetyldeoxyvalenol, neosolaniol, fusarenon-X, diacetoxyscirpenol, HT-2 toxin and T-2 toxin), aflatoxins (aflatoxin-B-1, aflaxoin-G(1) and aflatoxin-G(2)). Alternaria toxins (alternariol, alternariol methyl ether and altenuene), fumonisins (fumonisin-B-1, fumonisin-B-2 and fumonisin-B-3), ochratoxin A, zearalenone, beauvericin and sterigmatocystin. Optimization of the stimulataneous extraction of these toxins and the sample pretreatment procedure, as well as method validation were performed on maca (Lepidium meyenii) food supplements. The results indicated that the solvent mixture ethyl acetate/formic acid (95:5, v/v) n-hexane was applied as partial clean-up step to remove excess of co-extracted non-polar components. Further clean-up was performed on Oasis HLB(TM) cartidges. Samples were analysed using an Acquity UPLC system coupled to a Micromass Quattro Micro triple quadrupole mass spectrometer equipped with an electrospray interface operated in the positive-ion mode. Limits of detection and quantification were in the range of 0.3-30 ng g(-1) and 1-100 ng g(-1), respectively. Recovery yields were above 60% for most of the analytes, except for different food supplements such as soy (Glycine max) isoflavones, St John's wort (Hypericum perforatum), garlic (Allium sativum), Ginkgo biloba, and black radish (Raphanus niger) demonstrated the general applicability of the method. Due to different matrix effects observed in different food supplement samples, the standard addition approach was applied to perform correct quantitative analysis. In 56 out of 62 samples analysed, none of the 23 mycotoxins investigated was detected. Positive samples contained at least one of the toxins fumonisin-B-1, fumonisin-B-2, fumonisin-B-3 and ochratoxin A. [less ▲]

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See detailAnalysis of EU priority polycyclic aromatic hydrocarbons in food supplements using high performance liquid chromatography coupled to an ultraviolet, diode array or fluorescence detector
Danyi, Sophie ULg; Brose, François ULg; Brasseur, Catherine ULg et al

in Analytica Chimica Acta (2009), 633

High performance liquid chromatography coupled to an ultraviolet, diode array or fluorescence detector (HPLC/UV-FLD) has been used to set up a method to detect the 15(+1) EU priority polycyclic aromatic ... [more ▼]

High performance liquid chromatography coupled to an ultraviolet, diode array or fluorescence detector (HPLC/UV-FLD) has been used to set up a method to detect the 15(+1) EU priority polycyclic aromatic hydrocarbons (PAHs) in food supplements covering the categories of dried plants and plant extracts excluding oily products. A mini validation was performed and the following parameters have been determined: limit of detection, limit of quantification, precision, recovery and linearity. They were in close agreement with quality criteria described in the Commission Regulation (EC) No 333/2007 concerning the PAH benzo[a]pyrene in foodstuffs, except the not fluorescent cyclopenta[c,d]pyrene for which the UV detection leads to a higher limit of detection. Analysis of twenty commercial food supplements covering mainly the class of dried plants was performed to evaluate their PAHs contamination levels and to test the applicability of the method to various plant matrices. Fifty percent of analyzed samples showed concentration exceeding 2 gkg−1 for one or more PAHs. [less ▲]

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See detailFood flavonoid aryl hydrocarbon receptor-mediated agonistic/antagonistic/synergic activities in human and rat reporter gene assays
Van Der Heiden, Edwige ULg; Bechoux, Nathalie ULg; Muller, Marc ULg et al

in Analytica Chimica Acta (2009), 637

Aryl hydrocarbon receptor (AhR) is a ligand-activated transcription factor mediating the adverse effects of dioxins and polycyclic aromatic hydrocarbons (PAHs). In this study, we investigated the genetic ... [more ▼]

Aryl hydrocarbon receptor (AhR) is a ligand-activated transcription factor mediating the adverse effects of dioxins and polycyclic aromatic hydrocarbons (PAHs). In this study, we investigated the genetic-, time-, dose-, species- and tissue-dependent AhR-mediated agonistic/ antagonistic activities of three food flavonoids: quercetin, chrysin and genistein. To that end, four stably transfected cell lines were used in cell-based luciferase reporter gene assays: three lines were transformed with the ptKLuc vector harbouring four dioxinresponsive elements (DREs) upstream of the thymidine kinase promoter and the luciferase gene (HepG2-Luc, T-47D-Luc and H4IIE-ULg). The fourth is a patented cell line transformed with a different construct: H4IIE DR-CALUX®. Both H4IIE cells were compared for their genetic construction. Human hepatoma (HepG2-Luc) and human breast tumour (T-47D-Luc) cells were compared for tissue-dependent effects. Rat hepatoma (H4IIE-ULg) and human hepatoma (HepG2-Luc) cellswere compared for species-dependent activities.We concluded that quercetin, chrysin and genistein act in a time-, dose-, species- and tissue-specific way. For example, genistein displayed agonistic activities when exposed to rat hepatoma cells during 6h but not after 24 h. Flavonoids displayed agonistic/antagonistic activities in human breast tumour cells, depending on the exposure time, while in human hepatoma cells, only antagonistic activities of flavonoids were measured. In addition, we report, in all the cells, a synergy between an isoflavone and two food contaminants; the 2,3,7,8-tetrachlorodibenzop- dioxin and 3-methylcholanthrene, a PAH. In rat cells, this synergy occurred when cells were exposed to flavonoids and contaminant for 6h, while it was observed in human cells only after 24 h. [less ▲]

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See detailDetection of glucocorticoid bioactivity in bovine urine samples using a reporter gene assay
Connolly, Lisa; Cai, Kai; Van Der Heiden, Edwige ULg et al

in Analytica Chimica Acta (2009), 637

The illegal use of anabolic substances in the meat producing industry is an ongoing problem due to the continual production of new synthetic compounds and/or the practice of lowlevel cocktail ... [more ▼]

The illegal use of anabolic substances in the meat producing industry is an ongoing problem due to the continual production of new synthetic compounds and/or the practice of lowlevel cocktail administration to avoid detection by the surveillance schemes of EU member states National Plan surveillance systems. We present a highly sensitive reporter gene assay and sample extraction procedure based on a two step solid phase extraction and high performance liquid chromatography, developed for the detection of glucocorticoid abuse in bovine urine. The assay is capable of detecting compounds with glucocorticoid bioactivity and is extremely sensitive with an EC50 of 0.79 ngmL−1 for dexamethasone. New or unknown compounds with glucocorticoid bioactivity and low-level cocktail mixtures are detectable by this assay. Cross-reactivity data for a range of 11 -hydroxyglucocorticoids has been provided. This assay shows low interference from the 11-keto prohormones and other steroidal hormones. The assay may be suitable for application in other matrices such as hair. In conclusion this screening assay offers advantages over existing analytical techniques. [less ▲]

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See detailModulation of cytochrome P450 1A1 expression and activity in intestinal Caco-2 cells by components of Ginkgo biloba-based dietary supplements
Ribonnet, Laurence; Callebaut, Alfons; Scippo, Marie-Louise ULg et al

in Toxicology Letters (2009), 189

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See detailAssessment of the chemical contamination in home-produced eggs in Belgium: General overview of the CONTEGG study
Van Overmeire, Ilse; Pussemier, Luc; Wageneers, Nadia et al

in Science of the Total Environment (2009), 407(15), 4403-4410

This overview paper describes a study conducted for the Belgian Federal Public Service of Health, Food Chain Safety and Environment during 2006–2007. Home-produced eggs from Belgian private owners of hens ... [more ▼]

This overview paper describes a study conducted for the Belgian Federal Public Service of Health, Food Chain Safety and Environment during 2006–2007. Home-produced eggs from Belgian private owners of hens were included in a large study aiming to determine concentration levels of various environmental contaminants. Bymeans of the analyses of soil samples and of kitchen waste samples, obtained from the same locations, an investigation towards the possible sources of contaminants was possible. Eggs, soils, faeces and kitchen waste samples were checked for the presence of dioxins, PCBs (including dioxin-like PCBs), organochlorine pesticides, trace elements, PAHs, brominated flame retardants and mycotoxins. The study design, sampling methodology and primary conclusions of the study are given. It was found that in some cases dioxin-like compounds were present at levels that are of concern for the health of the egg consumers. Therefore, measures to limit their contamination in eggs, produced by hens of private owners, were proposed and deserve further attentio [less ▲]

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See detailAcetylcholinesterase activity as a biomarker of exposure to antibiotics and pesticides in the black tiger shrimp (Penaeus monodon).
Tu, Huynh Thi; Silvestre, Frederic; Scippo, Marie-Louise ULg et al

in Ecotoxicology & Environmental Safety (2009), 72(5), 1463-70

This study aimed to assess the potentiality to use cholinesterase activity (ChE) in black tiger shrimp (Penaeus monodon) as a biomarker of exposure to 2 antibiotics (enrofloxacin, furazolidone) and 2 ... [more ▼]

This study aimed to assess the potentiality to use cholinesterase activity (ChE) in black tiger shrimp (Penaeus monodon) as a biomarker of exposure to 2 antibiotics (enrofloxacin, furazolidone) and 2 pesticides (endosulfan, deltamethrin), commonly used in Vietnamese farms. ChE from muscle and gills was first characterised using three different substrates and specific inhibitors. Results showed that both tissues possess only one ChE which displays the typical properties of an acetylcholinesterase (AChE). In a second part, shrimp (average weight of 8.8-10 g) were fed with medicated-feed containing 4g enrofloxacin (quinolone) or furazolidone (nitrofuran)/kg for 7 days, or exposed to 3 actual concentrations of endosulfan (0, 0.009, 0.09, 0.9 microg/L) or deltamethrin (0, 0.0007, 0.007, 0.07 microg/L) for 4 days. After treatment, animals were decontaminated during 7 days. We observed that AChE activity in muscle was not significantly affected in shrimp fed with enrofloxacin or furazolidone, while it significantly decreased (up to 28%) in gills of shrimp fed with furazolidone. Following endosulfan and deltamethrin exposure, no significant changes in AChE activity were observed in gills. However, a significant decrease occurred in muscle after 4 days exposure (inhibition of 30% and 49% at 0.9 microg/L endosulfan and 0.07 microg/L deltamethrin, respectively). While muscle AChE activity should be assessed to point out endosulfan or deltamethrin exposure, gill AChE activity impairment could indicate an exposure to furazolidone. The present study underlines the benefits to use AChE as a biomarker of chemotherapeutics as part of an integrated aquaculture management to reach industry sustainability. [less ▲]

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See detailEstablishing a Belgian Nutrition Society (BNS): Filling The Void
Cani, Patrice; Clarys, Peter; Clinquart, Antoine ULg et al

in Archives of Public Health (2009), 67

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See detailEuropean legislation on methods for antibiotics detection in milk
Scippo, Marie-Louise ULg

Conference (2008, September 22)

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See detailCALUX bioassays
Scippo, Marie-Louise ULg

Conference (2008, July 08)

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See detailTime-, species- and tissue-dependent activity profiles of food flavonoids on the activation of the aryl hydrocarbon pathway
Van Der Heiden, Edwige ULg; Bechoux, Nathalie ULg; Sergent, Thérèse et al

in van Ginkel, L. A.; Bergwerff, A. A. (Eds.) Residues of Veterinary Drugs in Foods, Proceedings of the Euroresidue VI Conference (2008, May 19)

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See detailA new method for absolute quantification of allergens in food: the "Heavy Peptides method".
Fourdrilis, Séverine; Bourgeon, Cédric; Kirsch, Stéphanie ULg et al

Poster (2008)

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See detailPersistent organochlorine pollutants, dioxins and polychlorinated biphenyls
Scippo, Marie-Louise ULg; Eppe, Gauthier ULg; Saegerman, Claude ULg et al

in Contaminant and Residue Analysis, Comprehensive Analytical Chemistry of Elsevier (2008)

Detailed reference viewed: 118 (32 ULg)
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See detailDetection of glucocorticoid bioactivity in bovine urine samples using a reporter gene assay
Connolly, Lisa; Kai, C.; Van Der Heiden, Edwige ULg et al

in Proceedings of the Euroresidue VI Conference (2008)

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See detailTools to detect endocrine disruptors in the food chain
Scippo, Marie-Louise ULg

Scientific conference (2007, May)

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