References of "Sandersen, Charlotte"
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See detailGene expression profiling from leukocytes of horses affected by osteochondrosis.
Serteyn, Didier ULg; Piquemal, D.; Vander Heyden, Laurent ULg et al

in Journal of Orthopaedic Research (2010)

Osteochondrosis (OC) is a developmental disease that affects growing horses and that severely affects their ability to perform. The genetic basis of its pathogenesis is poorly understood. The aim of the ... [more ▼]

Osteochondrosis (OC) is a developmental disease that affects growing horses and that severely affects their ability to perform. The genetic basis of its pathogenesis is poorly understood. The aim of the study was to analyze the transcript profile of leukocytes from horses affected with OC. Two transcriptome libraries were constructed from leukocytes of OC-affected and non-OC-affected horses using digital gene expression analysis (DGE) and real-time PCR. Statistical analysis allowed selection of 1,008 tags upregulated in the non-OC-affected group and 1,545 tags upregulated in the OC-affected group. Among these genes, 16 regulated genes and 5 housekeeping genes were selected. Metabolic pathways analysis showed an obvious dysregulation of several signaling pathways related to cartilage formation or cartilage repair, including Wnt, Indian hedgehog, and TGF-beta signaling. Other genes, including ISG, ApoB, MGAT4, and TBC1D9, showed a significantly different expression between groups. These genes may play a role in high carbohydrate diet, abnormal insulin metabolism, or inflammation, mechanisms suspected to be involved in OC. This DGE analysis of the transcript profile of leukocytes from OC-affected horses demonstrated significant differences in comparison to the control library. These results open new perspectives for the understanding of equine OC. (c) 2010 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res. [less ▲]

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See detailEvaluation of the influence of age on pulmonary arterial pressure by use of right ventricular catheterization, pulsed-wave Doppler echocardiography, and pulsed-wave tissue Doppler imaging in healthy Beagles.
Mercier, Elise ULg; Mathieu, Myrielle; Sandersen, Charlotte ULg et al

in American Journal of Veterinary Research (2010), 71(8), 891-7

OBJECTIVE: To assess the influence of age on pulmonary hemodynamics and hemorheological properties in healthy dogs. ANIMALS: 14 healthy Beagles. PROCEDURES: Dogs were placed in 2 age groups as follows ... [more ▼]

OBJECTIVE: To assess the influence of age on pulmonary hemodynamics and hemorheological properties in healthy dogs. ANIMALS: 14 healthy Beagles. PROCEDURES: Dogs were placed in 2 age groups as follows: young dogs (<or= 5 years old; n = 8) and old dogs (>or= 8 years old; 6). Hematologic characteristics, plasma total protein and fibrinogen concentrations, and blood viscosity were measured. Systolic time intervals of pulmonary blood flow were recorded by pulsed-wave Doppler echocardiography. Early (E') and late (A') diastolic myocardial velocities, isovolumic contraction velocity, and systolic myocardial velocity of the free tricuspid annulus were recorded by pulsed-wave tissue Doppler imaging (TDI). Dogs were anesthetized and pulmonary arterial pressures (PAP) and cardiac output were recorded with a pediatric thermodilution Swan-Ganz catheter. RESULTS: Hemorheological measurements were not different between the 2 groups. Systolic, mean, and diastolic PAP were higher in old dogs, compared with values in young dogs; this difference was attributed to a high pulmonary vascular resistance and low arterial compliance in old dogs. Systolic time intervals of pulmonary blood flow stayed unchanged. The A' wave of the free tricuspid annulus was increased in old, compared with that young dogs, and the E':A' ratio was decreased. Pulmonary vascular resistance was inversely correlated with compliance. CONCLUSIONS AND CLINICAL RELEVANCE: The age of dogs should be taken into account when interpreting pulmonary hemodynamic results and TDI variables of right ventricular diastolic function. [less ▲]

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See detailDevelopment of an enzyme-linked immunosorbent assay for equine neutrophil elastase measurement in blood: Preliminary application to colic cases.
de la Rebière de Pouyade, Geoffroy ULg; Franck, Thierry ULg; Salciccia, Alexandra ULg et al

in Veterinary immunology and immunopathology (2010)

Equine neutrophil elastase (NE) is a protease released in inflammatory diseases and participating in tissue destruction. To measure NE in horse plasma to assess its role in pathological conditions, we ... [more ▼]

Equine neutrophil elastase (NE) is a protease released in inflammatory diseases and participating in tissue destruction. To measure NE in horse plasma to assess its role in pathological conditions, we purified elastase from equine neutrophils by a double step chromatography and obtained a pure protein of 27kDa, 4kDa smaller than the NE 2A previously purified (Scudamore et al., 1993; Dagleish et al., 1999), which was likely to be NE 2B. We developed an ELISA by using two specific polyclonal antibodies obtained from rabbit and guinea pig. The sandwich complex was detected using a secondary antibody conjugated to alkaline phosphatase. The ELISA showed good precision and accuracy, with intra- and inter-assay coefficients of variation below 10% for equine NE concentrations ranging from 1.875 to 60ng/ml. A stable plasma NE value, unaffected by the delay of centrifugation (over 4h), was obtained with plasma from EDTA anticoagulated blood. The mean value (+/-SEM) measured in 37 healthy horses was 32.53+/-4.6ng/ml. NE level in plasma of horses with colic at the time of admission was significantly higher than in healthy horses. Our results indicate that the ELISA technique we developed to measure plasmatic NE is a powerful tool for studying the role of elastase in equine inflammatory disease. In future, the application will be extended to other equine biological fluids. [less ▲]

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See detailComparison of the Repeatability of Echocardiographic Measurements from Different Modes and Views in Horses of Various Breeds and Sizes
Al Haidar, Ali; Farnir, Frédéric ULg; Deleuze, Stefan ULg et al

in Journal of Equine Veterinary Science (2010), 30(6), 287-297

The objective of this study was to compare the repeatability of echocardiographic measurements obtained from different echocardiographic modes and views in healthy adult equids of various sizes, breeds ... [more ▼]

The objective of this study was to compare the repeatability of echocardiographic measurements obtained from different echocardiographic modes and views in healthy adult equids of various sizes, breeds and thorax shapes. Ten, free of cardiac disease, equids (body weight: 120 to 662 kg; age: 1 to 26 years) from various breeds were used in this study. Each animal was submitted to a standardized echocardiographic and Doppler protocol 3 times at 1 day interval. This protocol included the measurements of left and right ventricular, aortic, pulmonary, and left atrial parameters obtained from different views using the bidimensional (2D) or the motion (M) modes, and the measurement of several parameters of blood flow obtained from the pulsed wave Doppler mode. Repeatability of each measurement was estimated on the basis of the residual variance using a linear model and the coefficient of variation of repeated measurements. A two by two comparison of the repeatability of measurements performed in different views was performed using the residual variances in a variance ratio F test. Results showed that repeatability of echocardiographic or Doppler measurements in equids of various sizes, breeds and thorax shapes are comparable to previously reported results in thoroughbred and standardbred horses. Left ventricular morphologic parameters showed a good repeatability in the classic M-mode right parasternal short axis (Sax) view at the level of the chordae tendinae, but the 2D-mode right parasternal long axis (LAx) four- chambers view appeared to offer an interesting alternative to measure them. This latter view also allowed obtaining the mostre repeatable measurement of right ventricular internal diameter. The left atrial diameter was maximal and most repeatable in the 2D-mode left parasternal long axisLAx four- chambers angled view, and the repeatability of the aortic diameter was best in the 2D-mode right parasternal long axisLAx five-chambers view. Finally, aortic systolic time intervals were more repeatable when measured from the Doppler mode as compared to the M-mode. ThusIn conclusion, best repeatability of echocardiographic measurements in horses could be optimized following an echocardiographicthe following protocol in equids: 1-M-mode right parasternal short axis view at the level of chordae tendinae, to measure left ventricular morphologic parameters, and 2D-mode right parasternal long axis four-chamber as an alternative view. 2-2D-mode right parasternal long axis five-chambersre, to measure the aortic diameter. 3-2D-mode left parasternal long axis four-chambersre angled view, to measure the left atrial diameter. [less ▲]

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See detailCardiologie des équidés
Amory, Hélène ULg; Sandersen, Charlotte ULg

Learning material (2010)

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See detailLa microbiopsie musculaire : un nouvel outil pour le suivi sportif et la détection précoce des dysfonctions musculaires
Votion, Dominique ULg; Fraipont, Audrey ULg; Robert, Céline et al

in 36ème Journée de la Recherche Equine (2010)

The objective of this study was to confirm the practical value of high-resolution respirometry (HRR) applied to biopsies to determine, in horses, the level of training, their athletic ability and for the ... [more ▼]

The objective of this study was to confirm the practical value of high-resolution respirometry (HRR) applied to biopsies to determine, in horses, the level of training, their athletic ability and for the early detection of muscular dysfunction. Materials and methods – The muscle mitochondrial respiration was determined by HRR in 20 endurance horses and 10 trotters sampled at the triceps brachii at different stages of their training. Results – Training increases mitochondrial respiration, in addition, the best performers had the highest rate of respiration. A trotter had abnormally low levels of muscle mitochondrial respiration for its level of training. This horse has presented several episodes of rhabdomyolysis during its racing season. Discussion – The biopsy is easily achievable by the attending veterinarian. This study shows that the athletic ability of horses is closely linked to respiratory muscle function, and suggests the value of HRR for performance prediction. In addition, the RHR has the ability to demonstrate mitochondrial dysfunction potentially responsible for exercise-induced rhabdomyolysis. [less ▲]

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See detailEuropean outbreak of atypical myopathy in the autumn 2009.
Van Galen, Gaby ULg; Amory, Hélène ULg; Busschers, Evita et al

in Journal of Veterinary Emergency & Critical Care (2010)

Background: Atypical myopathy (AM) is an acute, severe rhabdomyolysis occurring in grazing horses. Since In the beginning of October 2009, a new outbreak affects started in several European countries ... [more ▼]

Background: Atypical myopathy (AM) is an acute, severe rhabdomyolysis occurring in grazing horses. Since In the beginning of October 2009, a new outbreak affects started in several European countries. Geographic, demographic and clinical data of the reported cases in the month October 2009 are described. Key Findings: The survival rate over the month October 2009 was 25%. The most frequently observed clinical signs were congestedive mucous membranes, dyspnea, tachycardia, depression, weakness, stiffness, recumbency, trembling, transpirationsweating, and myoglobinuria. Non-survivors were significantly more often likely to be recumbent than survivors. Prognostic factors, symptomatic treatment and preventive measures are discussed. Significance: Slight differences were encountered during the described outbreak of AM in October 2009 compared to previous studies. Equine practitioners should be alert aware that previous epidemiological studies have shown that after a high prevalence this autumn, for new cases are likely to occur in the upcoming spring 2010. [less ▲]

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See detailEffect of intensive exercise on plasmatic neutrophil elastase level in eventing and endurance horses
Lejeune, Jean-Philippe ULg; Sandersen, Charlotte ULg; Votion, Dominique ULg et al

in Equine Veterinary Journal. Supplement (2010), 48

Reasons for performing the study – Intensive exercise induces a systemic inflammatory response characterized by an increase of blood neutrophil count and myeloperoxidase (MPO) release. Neutrophil elastase ... [more ▼]

Reasons for performing the study – Intensive exercise induces a systemic inflammatory response characterized by an increase of blood neutrophil count and myeloperoxidase (MPO) release. Neutrophil elastase (NE) could also contribute to tissues lesions by their proteinase activities. Objective – To compare plasmatic NE concentrations before and after different forms of intensive exercise. Materials and Methods – EDTA blood samples were taken from 51 eventing horses (EvH) and 32 endurance horses (EndH) were sampled before the race (T0). Blood sampling was performed 2 h (T1) after completing either phase D of a one or two star eventing competition (n=51) or a 120 or 160 km endurance race (n=32). Plasmatic NE and MPO were measured by a specific equine ELISA. Neutrophil counts and creatine kinase (CK) levels were also measured. A Wilcoxon test for paired samples was used to compare mean values of neutrophils, CK, MPO and NE at T0 and T1 in EvH and in EndH. Correlations were calculated between all the 4 parameters in EvH and EndH. Results – At T0, mean NE levels were 14.43 ± 3.63 ng/ml for EvH and 11.7 ± 2.11 ng/ml for EndH. The competition induced a significant increase of NE levels in (58.57 ± 24.06 ng/mL) EvH and (95.74 ± 22.70 ng/mL) EndH (p < 0,05). NE was significantly (p < 0,0001) correlated to MPO in EvH (r = 0.293) and EndH (0.594) and to CK (r = 0.297) in EndH (p<0.0001). Neutrophils, CK and MPO were significantly increased between T0 and T1 in both types of horses. Conclusions – Significant increase of NE was observed after intense exercise with a significant correlation between NE and MPO. The huge variability in MPO and ELT, indicates, that not all horses show the same intensity of systemic inflammatory response. [less ▲]

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See detailA case of a periarticular abscess and suppurative arthritis of the atlanto-occipital joint
Van Galen, Gaby ULg; Busoni, Valeria ULg; Sandersen, Charlotte ULg et al

in Equine Veterinary Education (2009), 21(7), 340-343

This case report describes a periarticular abscess and a suppurative arthritis of the atlanto-occipital joint in an adult horse. The horse showed a painful swelling localized on the atlanto-occipital ... [more ▼]

This case report describes a periarticular abscess and a suppurative arthritis of the atlanto-occipital joint in an adult horse. The horse showed a painful swelling localized on the atlanto-occipital region and ataxia in all four limbs. During his hospitalization the horse developed recumbency, dysphagia, facial paralysis and seizures. With the use of ultrasonography of the atlanto-occipital region a tentative diagnosis was made which was later confirmed by autopsy. Culture of the abscess revealed a Staphylococcus aureus. However, the horse had a history of recurrent fever, hypertrophy and abcessation of the submandibular lymph nodes, neck pain and dyspnoea for 2 months, suggestive for strangles. [less ▲]

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See detailA case of persistent hyperammonemia possibly due to a urea cycle enzyme deficiency
Van Galen, Gaby ULg; Sandersen, Charlotte ULg; Bolen, Géraldine ULg et al

in Proceedings of the 10th Annual Congress of the World Equine Veterinary Association (WEVA) (2009)

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See detailA comparison between pulse contour analysis and Doppler echocardiographic measurements of cardiac output in awake horses
Guidi, E; Amory, Hélène ULg; Borde, Laura ULg et al

in In Proceedings of the 3rd Annual Congress of the European College of Equine Internal Medicine (ECEIM) (2009)

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See detailEffect of breed on Doppler echocardiographic measurements in equids
Al Haidar, A; Sandersen, Charlotte ULg; Van Erck, Emmanuelle et al

in Proceedings of the 3rd Annual Congress of the European College of Equine Internal Medicine (ECEIM) (2009)

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See detailDie exokrine Pankreasfunktion beim kranken Pferd
Sandersen, Charlotte ULg; Serteyn, Didier ULg; Grulke, Sigrid ULg

in Vervuert; Achenbach; Gäbel (Eds.) et al LBH, Proccedings 5. Leipziger Tieraerzte Tag (2009)

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See detailCardiac Power Output during Dobutamine Stress
Sandersen, Charlotte ULg; Mc Entee, Kathleen ULg; Deleuze, Stefan ULg et al

in Journal of Equine Veterinary Science (2009)

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See detailEpidemiology of trace elements deficiencies in Belgian beef and dairy cattle herds.
Guyot, Hugues ULg; Saegerman, Claude ULg; Lebreton, Pascal et al

in Journal of Trace Elements in Medicine and Biology (2009), 23(2), 116-23

Selenium (Se), iodine (I), zinc (Zn) and copper (Cu) deficiencies in cattle have been reported in Europe. These deficiencies are often associated with diseases. The aim of the study was to assess trace ... [more ▼]

Selenium (Se), iodine (I), zinc (Zn) and copper (Cu) deficiencies in cattle have been reported in Europe. These deficiencies are often associated with diseases. The aim of the study was to assess trace element status in Belgian cattle herds showing pathologies and to compare them to healthy cattle herds. Eighty-two beef herds with pathologies, 11 healthy beef herds, 65 dairy herds with pathologies and 20 healthy dairy herds were studied during barn period. Blood and/or milk samples were taken in healthy animals. Plasma Zn, Cu, inorganic I (PII) and activity of glutathione peroxidase in erythrocytes (GPX) were assayed. In milk, I concentration was measured. Data about pathologies and nutrition in the herds were collected. According to defined thresholds, it appeared that a large proportion of deficient herds belonged to "sick" group of herds. This conclusion was supported by the mean value of trace elements and by the fact that a majority of individual values of trace elements was below the threshold. Dairy herds had mean values of trace elements higher than beef herds. More concentrates and minerals were used in healthy herds versus "sick" herds. These feed supplements were also used more often in dairy herds, compared to beef herds. Trace elements deficiencies are present in cattle herds in Belgium and are linked to diseases. Nutrition plays a major role in the trace elements status. [less ▲]

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See detailEvaluation of sedation depth and quality of performed procedures following administration of α2-agonists combined or not with butorphanol in horses.
Barbry, Stephanie; Verwilghen, Denis ULg; Van Galen, Gaby ULg et al

Poster (2008, October)

Several molecules available in equine medicine provide profound sedation and good analgesia when performing diagnostic or surgical procedures. They differ slightly in degree of action, as well in time as ... [more ▼]

Several molecules available in equine medicine provide profound sedation and good analgesia when performing diagnostic or surgical procedures. They differ slightly in degree of action, as well in time as in depth. The aim of this study was to evaluate the depth of sedation and quality of performing a standardized procedure after injection of an 2-agonist alone or associated with butorphanol. Horses presented to the Equine Clinic of Liege University from December 2007 to June 2008 for an endoscopic, dental, radiological or ophthalmological examination of which owner and clinician agreed to include the horse in the study were retained. All horses (n=133) were sedated in a randomized double blind fashion using one of the six following protocols: detomidine (D) (0.01mg/kg IV), romifidine (R) (0.04mg/kg IV), or xylazine (X) (0.5mg/kg IV) alone or associated with butorphanol (B) (0.02mg/kg IV). Stability, head position and the reaction to a stimulus (auditive and visual) were evaluated before the start of the procedure. An overall subjective grade using a 10 cm line scale was given to evaluate sedation depth (ranging from no effect to maximal depth on line) and the quality of the performed procedure (ranging from not realised to ideal examination). For each specific procedure, the same clinician experienced in his particular field evaluated the parameters. Data were related by a variance analysis using SAS system and considered significant if P<0.01. The used protocols significantly influenced both sedation depth and examination quality. Sedation depth increased significantly when B was added in all the three 2-agonists used (mean ± SD): D: 6.46±0.33; D+B: 7.06±0.37; R: 4.93±0.46; R+B: 7.04±0.45; X: 4.94±0.43; X+B: 7.75±0.40. Detomidine provided a significantly better quality of the examination compared to romifidine and xylazine. The addiction of butorphanol significantly decreased the examination quality when added to detomidine and increased the quality when combined with romifidine and xylazine. Mean (± SD) of the quality of performed procedures: D: 8.41±0.54; D+B: 6.06±0.64; R: 6.68±0.71; R+B: 7.16±0.67; X: 4.47±0.67; X+B: 4.52±0.64. Comparison of sedation depth and quality of performed examination revealed an increase in quality of examination scores with increasing depth of sedation until a certain level. As from sedation depths with scores of 8 or more, a decrease in quality of examination was encountered. This report shows the important effect of butorphanol addition on sedation. However, increasing sedation depth was not necessarily linked to a better quality of procedure, notably due to difficulties in handling strongly sedated horses for the procedures performed in this study. [less ▲]

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