References of "Saegerman, Claude"
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See detailBluetongue control--a new challenge for Europe.
Makoschey, Birgit; Beer, M.; Zientara, S. et al

in Berliner und Munchener Tierarztliche Wochenschrift (2008), 121(7-8), 306-13

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See detailEstimation préliminaire de l’impact économique induit par l’infection naturelle par le sérotype 8 du virus de la fièvre catarrhale ovine (FCO) en élevage ovin
Saegerman, Claude ULg; Raes, M.; Uyttenhoef, Aude ULg et al

in Epidémiologie et Santé Animale (2008), 54

In Northern Europe, bluetongue caused by the bluetongue virus, serotype 8 (BTV-8), emerged in August 2006 and numerous ruminant herds were found to be affected in 2007. An epidemiological postal survey ... [more ▼]

In Northern Europe, bluetongue caused by the bluetongue virus, serotype 8 (BTV-8), emerged in August 2006 and numerous ruminant herds were found to be affected in 2007. An epidemiological postal survey was sent to 502 sheep holders with the help of the sheep and goats inter-professional federation (called FICOW). A total of 18% of holders responded (mainly meat holders). These holders market their animals on short circuit (direct sales). Despite morbidity and mortality, animal sterility (still not quantified) and lower growth, costs of animal treatment were important (sick animals and/or animals living with sick animals): minimum = 2 €/animal treated; maximum = 135 € / animal treated; mean = 26 €/animal treated and median = 20 €/animal treated. Except for rams, the price of animals (for reproduction and meat) has not varied significantly while the survey was conducted. A second postal survey is programmed after the lambing period to assess reproductive and other economic losses that could not be quantified by the time this surveywas run. [less ▲]

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See detailVegetative endocarditis in the horse: retrospective study
Porter, Sarah ULg; Saegerman, Claude ULg; Van Galen, Gaby ULg et al

in Journal of Veterinary Internal Medicine (2008), 22(6), 1411-1416

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See detailCadmium in the food chain near non-ferrous metal production sites.
Vromman, V.; Saegerman, Claude ULg; Pussemier, L. et al

in Food Additives & Contaminants. Part A. Chemistry, Analysis, Control, Exposure & Risk Assessment (2008), 25(3), 293-301

Dietary cadmium (Cd) exposure was estimated for adults living in Cd-contaminated areas close to non-ferrous metal plants and compared with dietary Cd exposure in the general Belgian adult population. To ... [more ▼]

Dietary cadmium (Cd) exposure was estimated for adults living in Cd-contaminated areas close to non-ferrous metal plants and compared with dietary Cd exposure in the general Belgian adult population. To evaluate the contamination levels of locally produced food items, 35 fruit samples, 97 vegetable samples, 98 samples of potatoes and 53 samples of meat, liver and kidney of cattle, which had resided for more than 18 months in the contaminated area, were analyzed for Cd. Mean Cd concentrations in fruit and vegetables were 1.1- to 9-fold higher than in samples from other regions at ambient Cd levels. Mean Cd concentrations in bovine meat, liver and kidney were 2-fold higher compared to samples from animals in other regions of Belgium. The estimated dietary intake was 31.3 and 63.3 microg day(-1) for average and large consumers, respectively, in the contaminated area, compared to 17 and 38.3 microg day(-1), respectively, for the general adult population. Excessive consumption of locally produced food items in areas close to non-ferrous metal plants could result in Cd intake levels exceeding the provisional tolerable weekly intake (PTWI). [less ▲]

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See detailEpidémiologie de la fièvre catarrhale ovine en Europe : situation actuelle et perspectives
Saegerman, Claude ULg; Berkvens, D.; Philip, M. et al

in Epidémiologie et Santé Animale (2008), 54

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See detailFièvre catarrhale ovine : l’Europe au carrefour de l’enzootie
Saegerman, Claude ULg; Berkvens, D.; Mellor, P. S. et al

in Point Vétérinaire (2008), 290

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See detailLes réseaux d’épidémiosurveillance des maladies animales en Afrique francophone de l’Ouest et du Centre
Ouagal, M.; Hendrikx, P.; Berkvens, B. et al

in Revue Scientifique et Technique (International Office of Epizootics) (2008), 27(3), 689-702

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See detailA SYBR Green RT-PCR assay in single tube to detect human and bovine noroviruses and control for inhibition.
Scipioni, Alexandra ULg; Mauroy, Axel ULg; Ziant, Dominique ULg et al

in Virology journal (2008), 5

BACKGROUND: Noroviruses are single-stranded RNA viruses belonging to the family Caliciviridae. They are a major cause of epidemic and sporadic gastroenteritis in humans and clinical signs and lesions of ... [more ▼]

BACKGROUND: Noroviruses are single-stranded RNA viruses belonging to the family Caliciviridae. They are a major cause of epidemic and sporadic gastroenteritis in humans and clinical signs and lesions of gastroenteritis were reported in bovines. Due to their genetic proximity, potential zoonotic transmission or animal reservoir can be hypothesized for noroviruses. RT-PCR has become the "gold standard" for the detection of noroviruses in faecal and environmental samples. With such samples, the control for inhibition of the reaction during amplification and detection is crucial to avoid false negative results, which might otherwise not be detected. The aim of the reported method is to detect, with a SYBR Green technology, a broad range of noroviruses with a control for inhibition. RESULTS: A SYBR Green real-time RT-PCR assay was developed making use of a foreign internal RNA control added in the same tube. This assay is able to detect human and bovine noroviruses belonging to genogroups I, II and III and to distinguish between norovirus and internal control amplicons using melting curve analysis. A 10-fold dilution of samples appears to be the method of choice to remove inhibition. This assay was validated with human and bovine stool samples previously tested for norovirus by conventional RT-PCR. CONCLUSION: This SYBR Green real-time RT-PCR assay allows the detection of the most important human and bovine noroviruses in the same assay, and avoids false negative results making use of an internal control. Melting curves allow the discrimination between the internal control and norovirus amplicons. It gives preliminary information about the species of origin. The sensitivity of the developed assay is higher than conventional RT-PCR and a 10-fold dilution of samples showed a better efficiency and reproducibility to remove RT-PCR inhibition than addition of bovine serum albumin. [less ▲]

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See detailDetection and quantification of human and bovine novoviruses by a TaqMan RT-PCR assay with a control for inhibition.
Scipioni, Alexandra ULg; Bourgot, Isabelle; Mauroy, Axel ULg et al

in Molecular and Cellular Probes (2008), 22

Noroviruses are single-stranded RNA viruses belonging to the family Caliciviridae. They are a major cause of epidemic and sporadic gastroenteritis in humans and calves. Reverse transcription-polymerase ... [more ▼]

Noroviruses are single-stranded RNA viruses belonging to the family Caliciviridae. They are a major cause of epidemic and sporadic gastroenteritis in humans and calves. Reverse transcription-polymerase chain reaction (RT-PCR) has become the ‘‘gold standard’’ for detection of noroviruses in faecal and environmental samples. However, false negative results due to co-concentration of RT-PCR inhibitors are a continuous concern. A TaqMan real-time RT-PCR assay making use of a foreign internal RNA control and a RNA standard was developed. Very interestingly, this method is capable of detecting human noroviruses belonging to genogroups I and II, and bovine noroviruses belonging to genogroup III. Inhibitors were removed efficiently by 1/10 dilution of the sample or addition of bovine serum albumin to the RT-PCR mix. This assay was validated with human and bovine stool samples previously tested for norovirus by conventional RT-PCR. The ability to detect norovirus in stool samples that were negative by conventional RT-PCR assay demonstrate the higher sensitivity of the TaqMan assay compared to the conventional RT-PCR assay. This real-time RT-PCR assay allows the detection of both human and bovine noroviruses, avoids false negative results and is able to quantify the level of norovirus contamination. [less ▲]

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See detailRisk assessment of the re-emergence of bovine brucellosis/tuberculosis
Saegerman, Claude ULg; Porter, Sarah ULg; Humblet, Marie-France ULg

in Emerging animal diseases: from science to policy (2008)

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See detailCharacterization of the cattle serum antibody responses against TEM beta-lactamase and the nonimmunogenic Escherichia coli heat-stable enterotoxin (STaI)
Zervosen, Astrid ULg; Saegerman, Claude ULg; Antoniotti, Ingrid et al

in FEMS Immunology & Medical Microbiology (2008), 54(3), 319-329

In order to test the use of a subunit recombinant vaccine for its capacity to induce antibodies against the nonimmunogenic heat-stable enterotoxin STa from Escherichia coli and the TEM-1 beta-lactamase ... [more ▼]

In order to test the use of a subunit recombinant vaccine for its capacity to induce antibodies against the nonimmunogenic heat-stable enterotoxin STa from Escherichia coli and the TEM-1 beta-lactamase, cattle were immunized with a hybrid protein created by insertion of the STa sequence at position 197 of the TEM-1 beta-lactamase. Specific anti-STa IgG and IgG1 antibodies were detected at low levels, while no IgG2 antibodies were detected. In contrast, high levels of the different anti-TEM IgG subtypes were detected in cattle sera. In addition, beta-lactamase activity was inhibited by the sera. The presence of antibodies against STa and TEM-1 beta-lactamase was assessed in sera from 366 cattle taken from the field. No significant level of IgGs against the toxin or the TEM-1 was detected. A comparison of the antibody level between the immunized and the nonimmunized animals clearly demonstrated that STa was not able to induce a significant level of antibodies in the vaccinated animals. In contrast, a strong antibody response against TEM-1 beta-lactamase was demonstrated. [less ▲]

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See detailPersistent organochlorine pollutants, dioxins and polychlorinated biphenyls
Scippo, Marie-Louise ULg; Eppe, Gauthier ULg; Saegerman, Claude ULg et al

in Contaminant and Residue Analysis, Comprehensive Analytical Chemistry of Elsevier (2008)

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See detailBluetongue in captive yaks.
Mauroy, Axel ULg; Guyot, Hugues ULg; De Clercq, Kris et al

in Emerging Infectious Diseases (2008), 14(4), 675-6

In August 2006, several Northern European countries including Belgium reported their first cases of bluetongue (BT). Surprisingly, it was the first time that BT was diagnosed so far in the northern ... [more ▼]

In August 2006, several Northern European countries including Belgium reported their first cases of bluetongue (BT). Surprisingly, it was the first time that BT was diagnosed so far in the northern hemisphere (1). BT is a non contagious, arthropod borne animal disease. The causal virus belongs to the genus Orbivirus in the family Reoviridae. The genome of the bluetongue virus (BTV) consists of 10 segments of double-stranded RNA and 24 serotypes have been reported (2). Serotype 8 (BTV-8) was implied in the emergence in Belgium (3). All ruminant species are thought to be susceptible to BT (2) but lack of data remains for certain species. We report here laboratory confirmed clinical cases of BT in yaks. [less ▲]

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See detailEpizootológia modrého jazyka (Bluetongue) v Európskej únii
Saegerman, Claude ULg; Berkvens, D.; Mellor, P. S.

in Slovak Veterinary Journal (2008), 23(4), 217-220

Zhrnutie. Modrý jazyk (Bluetongue (BT)) je ochorenie podliehajúce hláseniu, ktoré vyvoláva značné socio-ekonomické znepokojenie a je veľmi závažné v súvislosti s medzinárodným obchodom so zvieratami a ... [more ▼]

Zhrnutie. Modrý jazyk (Bluetongue (BT)) je ochorenie podliehajúce hláseniu, ktoré vyvoláva značné socio-ekonomické znepokojenie a je veľmi závažné v súvislosti s medzinárodným obchodom so zvieratami a živočíšnymi produktmi. Pred rokom 1998, sa BT v Európe považoval za exotické ochorenie. Od roku 1998 až do roku 2005, bolo nepretržite prítomných v Stredomorskej kotline najmenej 6 BT vírusových kmeňov, patriacich do 5 sérotypov (BTV-1, BTV-2, BTV-4, BTV-9, a BTV-16). Od augusta 2006, spôsobuje BTV-8 v severnej Európe závažnú epizoóciu BT. Rozsiahle opätovné prepuknutie a rozšírenie infekcií v severnej Európe spôsobených BTV-8 v priebehu roku 2007 poukazuje na to, že požiadavky na etablovanie BTV môžu byť v tejto oblasti teraz splnené. Okrem toho, radiálne rozšírenie BTV-8 v Európe zvyšuje riziko pre stretnutie sa tohto sérotypu s inými sérotypmi, zvlášť tými, ktoré sa vyskytujú v Stredomorskej kotline, kde aktivita vektora trvá väčšinou počas celého roka. Táto okolnosť zvyšuje riziko pre preskupenie (reassortment) jednotlivých segmentov BTV génu. [less ▲]

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See detailBluetongue Epidemiology in the European Union
Saegerman, Claude ULg; Berkvens, D.; Mellor, P. S.

in Emerging Infectious Diseases (2008), 14(4), 539-544

Bluetongue (BT) is a reportable disease of considerable socioeconomic concern and of major importance in the international trade of animals and animal products. Before 1998, BT was considered an exotic ... [more ▼]

Bluetongue (BT) is a reportable disease of considerable socioeconomic concern and of major importance in the international trade of animals and animal products. Before 1998, BT was considered an exotic disease in Europe. From 1998 through 2005, at least 6 BT virus strains belonging to 5 serotypes (BTV-1, BTV-2, BTV-4, BTV-9, and BTV-16) were continuously present in the Mediterranean Basin. Since August 2006, BTV-8 has caused a severe epizootic of BT in northern Europe. The widespread recrudescence and extension of BTV-8 infections in northern Europe during 2007 suggest that requirements for BTV establishment may now be fulfilled in this area. In addition, the radial extension of BTV-8 across Europe increases the risk for an encounter between this serotype and others, particularly those that occur in the Mediterranean Basin, where vector activity continues for more of the year. This condition increases the risk for reassortment of individual BTV gene segments. [less ▲]

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See detailBluetongue: Epidemiology in the European Union
Saegerman, Claude ULg; Berkvens, D.; Mellor, P. S.

in Bluetongue in northern Europe (2008)

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See detailBluetongue: General introduction
Saegerman, Claude ULg; Revieriego-Gordejo, F.; Pastoret, Paul-Pierre ULg

in Bluetongue in northern Europe (2008)

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See detailClinical aspects of bluetongue in ruminants
Guyot, Hugues ULg; Mauroy, Axel ULg; Kirschvink, N. et al

in Bluetongue in northern Europe (2008)

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See detailConclusion: Lesson learning on bluetongue
Saegerman, Claude ULg; Revieriego-Gordejo, F.; Pastoret, Paul-Pierre ULg

in Bluetongue in northern Europe (2008)

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