References of "Saegerman, Claude"
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See detailMonitoring des animaux vivants : exemple d’un échantillonnage pour la détection des PCBs et des dioxines chez les bovins de boucherie en Belgique
Saegerman, Claude ULg; Boelaert, F.; Van Vlanderen, I. et al

in Epidémiologie et Santé Animale (2000), 38

In February 1999, a poisoning episode broke out in several industrial poultry farms in Belgium. The source of this contamination was found to be a stock of recycled fat that had been delivered by a firm ... [more ▼]

In February 1999, a poisoning episode broke out in several industrial poultry farms in Belgium. The source of this contamination was found to be a stock of recycled fat that had been delivered by a firm to several compound feed producers, between 19 and 31 January 1999. A very good correlation was observed between dioxins (PCDFs and PCDDs) and PCBs. Consequently a contamination mainly by PCBs was hypothesized. This finding made it possible to detect contaminated animals and animal products by dosage of the PCBs, more specifically by the 7 congeners with numbers 28, 52, 101, 118, 138, 153 and 180. The advantages of the dosage of PCBs compared to dioxins were its rapid test protocol and the fact that a larger number of laboratories could participate. In the cattle sector, 409 herds (0,81 p. cent) were submitted to the risk of feed contamination. The methodology used to detect a PCBs/dioxins contamination in the Belgian cattle population that was not submitted to the risk, is presented. This population is directly or indirectly destined for human consumption. It consisted in the systematic sampling of all calve fattening stations and all lots of exported bovines, and in the random sampling of slaughter cattle. This methodology is compared to the approach described in directive 96/23/CE. [less ▲]

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See detailEpidemiologische bewaking van boviene spongiforme encefalopathie in Belgïe: Jaaroverzicht voor 1998
Saegerman, Claude ULg; Dechamps, P.; Vanopdenbosch, E. et al

in Vlaams Diergeneeskundig Tijdschrift (2000), 69

In 1998 werden 6 runderen tussen de 54 en 71 maanden ouderdom, afkomstig van de provincies West-Vlaanderen (3 gevallen), Oost-Vlaanderen (2 gevallen) en Luik (1 geval), gediagnostiseerd als gevallen van ... [more ▼]

In 1998 werden 6 runderen tussen de 54 en 71 maanden ouderdom, afkomstig van de provincies West-Vlaanderen (3 gevallen), Oost-Vlaanderen (2 gevallen) en Luik (1 geval), gediagnostiseerd als gevallen van boviene spongiforme encephalopathie (BSE). De hypotheses betreffende de oorsprong van de infectie op zijn de volgende : het optreden van sporadische gevallen zonder duidelijk definieerbare oorzaak; de mogelijke kruiscontaminatie tussen voeder voor monogastrische dieren met daarin dierlijk meel en voeder voor herkauwers waarin geen dierlijk meel is verwerkt en dit tijdens het fabricatieproces, de stockage, het transport of de distributie; het gebruik van dierlijk beendermeel in het voeder voor runderen geproduceerd voor de ban (van kracht vanaf 27/7/1994). Algemeen kan men dus stellen dat in België de aanwezigheid van gecontamineerd diermeel als risicofactor voor BSE niet kan worden uitgesloten. De oorsprong van deze diermelen kon nog niet gedetermineerd worden. [less ▲]

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See detailDiagnosis of bovine brucellosis by skin test: conditions for the test and evaluation of its performance.
Saegerman, Claude ULg; Vo, T. K.; De Waele, L. et al

in Veterinary Record : Journal of the British Veterinary Association (1999), 145(8), 214-8

Brucellergene OCB (Rhone-Merieux) was used as an allergen to define the intrinsic parameters of a skin test and to compare its properties with serology for the diagnosis of bovine brucellosis. The skin ... [more ▼]

Brucellergene OCB (Rhone-Merieux) was used as an allergen to define the intrinsic parameters of a skin test and to compare its properties with serology for the diagnosis of bovine brucellosis. The skin test was also evaluated for its capacity to solve problems associated with false positive reactions in serological tests. The optimal reading delay for the skin test was 72 hours. The brucellosis allergic reaction was two to three times less intense than the tuberculosis allergic reaction. An increase of 1.1 mm or more in the skin thickness was therefore considered to be an adequate cut-off. The specificity calculated for 1192 brucellosis-free animals (including animals from brucellosis-free herds in which false positive serological reactions had been reported) was 99-83 per cent (95 per cent confidence interval [CI] 99-40 to 99-98 per cent). The sensitivity determined from 27 experimentally infected heifers ranged from 93 per cent (95 per cent CI 76 to 100 per cent) to 78 per cent (95 per cent CI 58 to 91 per cent) when measured respectively one and six months after the infection. Allergic reactions could be detected in vaccinated animals up to four-and-a-half years after the vaccination. On the other hand, no sensitisation was recorded in naive animals after up to eight monthly injections of the allergen. The skin test gave valuable information, in combination with the serological tests, in both acute and chronic brucellosis. The skin test discriminated brucellosis clearly from false positive serological reactions due to infections with Yersinia enterocolitica O9. [less ▲]

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See detailEpidémiosurveillance de la rage animale en Belgique : un seul cas détecté en 1998
Brochier, B.; Dechamps, P.; Costy, F. et al

in Annales de Médecine Vétérinaire (1999), 143

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See detailEtude rétrospective de l'incidence des troubles nereux rapportés et suspects d'encéphalopathie spongiforme transmissible chez les bovins en Belgique
Saegerman, Claude ULg; Claes, M.; Vanopdenbosch, E. et al

in Epidémiologie et Santé Animale (1999), 35

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See detailEpidémiosurveillance de l'encéphalopathie spongiforme bovine en Belgique : bilan de l'année 1998
Saegerman, Claude ULg; Dechamps, P.; Vanopdenbosch, E. et al

in Annales de Médecine Vétérinaire (1999), 143

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See detailBovine brucellosis diagnosis by skin test: conditions for its use and evaluation of its performance
Saegerman, Claude ULg; Vo, T. K. O.; De Waele, L. et al

in Veterinary Record : Journal of the British Veterinary Association (1999), 145

Brucellergene OCB (Rhône-Mérieux) was used as an allergen to define the intrinsic parameters of a skin test and to compare its properties with serology for the diagnosis of bovine brucellosis. The skin ... [more ▼]

Brucellergene OCB (Rhône-Mérieux) was used as an allergen to define the intrinsic parameters of a skin test and to compare its properties with serology for the diagnosis of bovine brucellosis. The skin test was also evaluated for its capacity to solve problems associated with false positive reactions in serological tests. The optimal reading delay for the skin test was 72 hours. The brucellosis allergic reaction was two to three times less intense than the tuberculosis allergic reaction. An increase of 1.1 mm or more in the skin thickness was therefore considered to be an adequate cut-off. The specificity calculated for 1192 brucellosis-free animals (including animals from brucellosis-free herds in which false positive serological reactions had been reported) was 99-83 per cent (95 per cent confidence interval [CI] 99-40 to 99-98 per cent). The sensitivity determined from 27 experimentally infected heifers ranged from 93 per cent (95 per cent CI 76 to 100 per cent) to 78 per cent (95 per cent CI 58 to 91 per cent) when measured respectively one and six months after the infection. Allergic reactions could be detected in vaccinated animals up to four-and-a-half years after the vaccination. On the other hand, no sensitisation was recorded in naïve animals after up to eight monthly injections of the allergen. The skin test gave valuable information, in combination with the serological tests, in both acute and chronic brucellosis. The skin test discriminated brucellosis clearly from false positive serological reactions due to infections with Yersinia enterocolitica O9. [less ▲]

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See detailLe premier cas d’encéphalopathie spongiforme bovine diagnostiqué en Belgique
Vanopdenbosch, E.; Dechamps, P.; Dufey, J. et al

in Annales de Médecine Vétérinaire (1998), 142

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See detailInfection of cattle with Yersinia enterocolitica O:9 a cause of the false positive serological reactions in bovine brucellosis diagnostic tests.
Weynants, V.; Tibor, A.; Denoel, P. A. et al

in Veterinary Microbiology (1996), 48(1-2), 101-12

During the last four years, an increasing number of cattle herds were classified positive by brucellosis screening tests in areas of Belgium and France free of the disease. No clinical symptom of ... [more ▼]

During the last four years, an increasing number of cattle herds were classified positive by brucellosis screening tests in areas of Belgium and France free of the disease. No clinical symptom of brucellosis was reported in these animals and no Brucella abortus strains were isolated. After two years, no brucellosis outbreak was registered in all of the herds concerned. On this basis, all the serological reactions observed were classified as false positive. An ELISA using Yersinia Outer membrane Proteins (YOPs) as antigens was developed in order to discriminate between a Yersinia enterocolitica O:9 infection and a Brucella abortus infection. Antibodies against YOPs were detected in sera from Y. enterocolitica O:9 experimentally infected cattle (n = 4) but not in sera from B. abortus experimentally infected cattle (n = 4). In a field study, 66.7% of the 174 serum samples from cattle presenting false positive serological reactions showed anti-YOPs antibodies whereas only 10% of 454 sera, classified negative by the brucellosis screening tests, showed anti-YOPs antibodies. Our results suggest that infections with Y. enterocolitica O:9 may cause false positive reactions in brucellosis testing. [less ▲]

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See detailInfection of cattle with Yersinia enterocolitica O:9 a cause of the false positive serological reactions in bovine brucellosis diagnostic tests.
Weynants, V.; Tibor, A.; Denoel, P. A. et al

in Veterinary Microbiology (1996), 48(1-2), 101-12

During the last four years, an increasing number of cattle herds were classified positive by brucellosis screening tests in areas of Belgium and France free of the disease. No clinical symptom of ... [more ▼]

During the last four years, an increasing number of cattle herds were classified positive by brucellosis screening tests in areas of Belgium and France free of the disease. No clinical symptom of brucellosis was reported in these animals and no Brucella abortus strains were isolated. After two years, no brucellosis outbreak was registered in all of the herds concerned. On this basis, all the serological reactions observed were classified as false positive. An ELISA using Yersinia Outer membrane Proteins (YOPs) as antigens was developed in order to discriminate between a Yersinia enterocolitica O:9 infection and a Brucella abortus infection. Antibodies against YOPs were detected in sera from Y. enterocolitica O:9 experimentally infected cattle (n = 4) but not in sera from B. abortus experimentally infected cattle (n = 4). In a field study, 66.7% of the 174 serum samples from cattle presenting false positive serological reactions showed anti-YOPs antibodies whereas only 10% of 454 sera, classified negative by the brucellosis screening tests, showed anti-YOPs antibodies. Our results suggest that infections with Y. enterocolitica O:9 may cause false positive reactions in brucellosis testing. [less ▲]

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See detailSpecific bovine brucellosis diagnosis based on in vitro antigen-specific gamma interferon production.
Weynants, V.; Godfroid, J.; Limbourg, B. et al

in Journal of Clinical Microbiology (1995), 33(3), 706-12

In order to improve the specificity of the diagnosis of bovine brucellosis, we developed a test which can be regarded as an in vitro correlate of the delayed-type hypersensitivity test (DTH). A mixture of ... [more ▼]

In order to improve the specificity of the diagnosis of bovine brucellosis, we developed a test which can be regarded as an in vitro correlate of the delayed-type hypersensitivity test (DTH). A mixture of cytoplasmic proteins from Brucella melitensis B115 was used as a specific antigenic stimulus in bovine whole blood culture. Supernatants harvested at 18 to 24 h after the in vitro antigenic stimulus were assayed for their gamma interferon (IFN-gamma) content by using a commercial sandwich enzyme-linked immunosorbent assay kit. The IFN-gamma assay was evaluated with 10 heifers during the course (80 days) of an experimental infection and with 14 cows from an ongoing brucellosis outbreak. All of these animals were slaughtered, and pertinent organs were subjected to classical bacteriological analyses. In addition, we analyzed 23 field cases in which false-positive serological reactions occurred. The IFN-gamma results were compared with those of the standard DTH and a battery of serological assays, and they were correlated with bacteriological data. Both for the experimental infection and for the field brucellosis outbreak, the IFN-gamma assay detected infection in more animals than any combination of the serological tests, and it detected infection earlier than these tests. Finally, none of the samples from cows showing false-positive serological reactions was classified as positive by the IFN-gamma assay, attesting to its specificity and to its usefulness in interpreting ambiguous serological results. A rapid and convenient alternative to the DTH, the IFN-gamma assay appears to be an ideal method that is complementary to the serological diagnosis protocols. [less ▲]

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See detailSpecific bovine brucellosis diagnosis based on in vitro antigen-specific gamma interferon production.
Weynants, V.; Godfroid, J.; Limbourg, B. et al

in Journal of Clinical Microbiology (1995), 33(3), 706-12

In order to improve the specificity of the diagnosis of bovine brucellosis, we developed a test which can be regarded as an in vitro correlate of the delayed-type hypersensitivity test (DTH). A mixture of ... [more ▼]

In order to improve the specificity of the diagnosis of bovine brucellosis, we developed a test which can be regarded as an in vitro correlate of the delayed-type hypersensitivity test (DTH). A mixture of cytoplasmic proteins from Brucella melitensis B115 was used as a specific antigenic stimulus in bovine whole blood culture. Supernatants harvested at 18 to 24 h after the in vitro antigenic stimulus were assayed for their gamma interferon (IFN-gamma) content by using a commercial sandwich enzyme-linked immunosorbent assay kit. The IFN-gamma assay was evaluated with 10 heifers during the course (80 days) of an experimental infection and with 14 cows from an ongoing brucellosis outbreak. All of these animals were slaughtered, and pertinent organs were subjected to classical bacteriological analyses. In addition, we analyzed 23 field cases in which false-positive serological reactions occurred. The IFN-gamma results were compared with those of the standard DTH and a battery of serological assays, and they were correlated with bacteriological data. Both for the experimental infection and for the field brucellosis outbreak, the IFN-gamma assay detected infection in more animals than any combination of the serological tests, and it detected infection earlier than these tests. Finally, none of the samples from cows showing false-positive serological reactions was classified as positive by the IFN-gamma assay, attesting to its specificity and to its usefulness in interpreting ambiguous serological results. A rapid and convenient alternative to the DTH, the IFN-gamma assay appears to be an ideal method that is complementary to the serological diagnosis protocols. [less ▲]

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See detailSerological and cutaneous testing of bovine tuberculosis with the A60 antigen complex from Mycobacterium bovis, strain Calmette-Guérin
Saegerman, Claude ULg

in Preventive Veterinary Medicine (1995), 23

A60, a major thermostable macromolecular antigen complex of Mycobacterium bovis strain Calmette-Guerin (BCG), is immunodominant in tuberculosis and able to elicit both humoral and cellular immune ... [more ▼]

A60, a major thermostable macromolecular antigen complex of Mycobacterium bovis strain Calmette-Guerin (BCG), is immunodominant in tuberculosis and able to elicit both humoral and cellular immune reactions in infected hosts. A60-based ELISA and cutaneous tests have been used, in conjunction with the PPD-based skin reaction, in a control group of healthy animals, and in a herd including tuberculous animals. Cutaneous testing with A60 yielded results comparable with those with PPD: both were negative with control cattle and positive with infected animals. Moreover, comparative cutaneous testing with avian tuberculin yielded similar results with PPD and A60. When animals from the infected herd were tested with both avian and bovine sensitins, 54% of cattle were diagnosed as fully positive, 26% suspect, and 20% negative. Serological analysis with the A60-ELISA of part of the infected herd yielded 74% positive, 21% suspect and 5% negative results. Thus, positivity was 74% for serological analysis and 54% for cutaneous testing, whereas positive plus suspect results were 95% for serological analysis and 80% for cutaneous testing. It can be concluded that A60 can be used in place of PPD for cutaneous testing in cattle, and that the diagnostic value of the A60-ELISA is superior to that of the PPD-cutaneous test. [less ▲]

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See detailGestion d'un épisode de peste porcine classique en Belgique
Vanthemsche, P.; Saegerman, Claude ULg

in Epidémiologie et Santé Animale (1994), 26

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See detailBrucellose enzootique à Brucella suis biotype 2 chez le sanglier (sus scrofa) en Belgique
Godfroid, J.; Michel, P.; Uytterhaegen, L. et al

in Annales de Médecine Vétérinaire (1994), 138

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See detailMonitoring of Yersinia enterocolitica in murine and bovine feces on the basis of the chromosomally integrated luxAB marker gene
Kaniga, K.; Sory, M. P.; Delor, I. et al

in Applied and Environmental Microbiology (1992), 58(3), 1024-1026

We previously integrated the luxAB gene into the Yersinia enterocolitica chromosome. In this article, we assessed, by luminometry, the survival of the engineered strain KNG1024 in the digestive tracts of ... [more ▼]

We previously integrated the luxAB gene into the Yersinia enterocolitica chromosome. In this article, we assessed, by luminometry, the survival of the engineered strain KNG1024 in the digestive tracts of mice and cows. In situ detection and a count of the released strain were performed on feces from orally inoculated BALB/c mice for 24 days. This method is a rapid and reliable system for long-term monitoring of genetically engineered bacteria. In cow feces, the count of Y. enterocolitica ranged from 210 to 6,000 CFU/g of feces. This very low count was not detectable by direct luminometry [less ▲]

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