Evaluation of the long-term barrier effect of commercial resorbable guided tissue regenerative membranes : an in vitro study using human gingival fibroblasts

Poster (2009)

Introduction The first part of the study devoted to guided tissue regenerative membranes was focused on a better understanding of the physicochemical and mechanical properties of commercial materials. The ... [more ▼]

Introduction The first part of the study devoted to guided tissue regenerative membranes was focused on a better understanding of the physicochemical and mechanical properties of commercial materials. The second objective of our study was to develop an in vitro device able to measure the long-term barrier effect of resorbable membranes. After the development of this new device, experiments were realized to characterize the long-term behaviour of commercially membranes with human gingival fibroblasts (HGF). Materials and methods The use of human gingival fibroblastic cells was chosen to get closer to biological conditions. Some gingival explants were removed in young and non-smoking healthy patients. From these explants, fibroblastic cells were isolated and cultivated. These cells will be able to be used between the third and the sixth passage. Resorbable membranes were chosen because they don’t require a second surgical operation. There are made of polyesters or collagen. A system based on inserts was developed in order to follow the degradation of membranes and the migration of cells across the material. The membrane was cut into 8 mm diameter punches and set in the bottom of the system. Once the whole was put together, it is laced into a 12 wells plate culture. First, the plates were put in an incubator at 37°C, during times ranging from 24 hours to several months. The barrier effect was then measured to reflect the gradual increase of permeability of each membrane. For this purpose, HGF were seeded on the different samples. The top of the bottle and the bottom of the well were then filled with culture medium. Non degradable synthetic Bioflex membranes were chosen as control samples which don't let pass cells (porosity : 0,4 µm). After 48 hours of incubation in the presence of cells, pictures of cells on membranes and in the bottom of wells were taken with an optic microscope. Viability tests (MTS) were then realized on membranes to evaluate cells proliferation and in the bottom of wells to measure barrier effect. Finally, the morphology of cells on selected membranes was characterized by Scanning Electron Microscopy. Conclusion Proliferation results correspond to data published by several authors. Furthermore, the barrier effect times found in the present study are similar to barrier effect times demonstrated in in vivo studies and announced by manufacturers. In conclusion, the finalized system is adapted to the analysis of long-term barrier effect of commercial GTR membranes. This system will be tested with synthetic bioresorbable membranes made of copolymers. [less ▲]

3-D guided bone regeneration using autogenous bone block as space maintainer for bone augmentation in the anterior maxilla.

Poster (2007, October)

A minimally invasive approach for horizontal bone regeneration of the anterior maxilla.

Poster (2007, May)

Temperature rise during photo-activated disinfection of root canals.

in Lasers in Medical Science (2007)

The aim of this study was to determine whether it is safe to use photo-activated disinfection (PAD) during root canal treatment without heating the periodontal tissues. Root canals of 30 freshly extracted ... [more ▼]

The aim of this study was to determine whether it is safe to use photo-activated disinfection (PAD) during root canal treatment without heating the periodontal tissues. Root canals of 30 freshly extracted single-rooted teeth were prepared using ProFiles up to size ISO 40 and then filled with photo-sensitiser: tolonium blue (1.2 mg/l). The 635 nm diode laser was used with the manufacturer's endo-tip. Samples were irradiated for 150 s (output power 100 mW, approximate energy density 106.16 J/cm(2)). Temperatures were recorded at working length on the external root surface. After 150 s of PAD irradiation, the average temperature rise was 0.16 +/- 0.08 degrees C. All values were lower than the 7 degrees C safety level for periodontal injury. It was concluded that, regarding the temperature increase, the use of PAD in root canals could be considered harmless for periodontal tissues. [less ▲]

Placement of non-submerged implants simultaneously with sinus augmentation with bovine HA

in Clinical Oral Implants Research (2005, September)

The best solution for you patient. A Biological Approach for Aesthetic Implant Dentistry.

Poster (2005, May)

Soft tissue attachment formation at titanium abutments in humans: a new model

in Journal of Dental Research (2003, December), 82(Sp. Iss. C), 510

Repercussions cardio-vasculaires des infections parodontales

Article for general public (2002)

De plus en plus de preuves s'accumulent montrant que les infections parodontales sont des facteurs de risque indépendants pour certaines pathologies systémiques dont les affections cardiovasculaires ... [more ▼]

De plus en plus de preuves s'accumulent montrant que les infections parodontales sont des facteurs de risque indépendants pour certaines pathologies systémiques dont les affections cardiovasculaires: fréquence de parodontite 2,6x plus grande chez les patients atteints d'ischémie cérébrales, risque de décès d'origine cardiovasculaire doublé en cas de parodontite chez des hommes de moins de 50 ans. [less ▲]

Repercussions systémiques des infections parodontales: définition d'un nouvel index parodontal

in Monde Dentaire (Le) (2001), 107

La santé parodontale: une clef de la santé bucco-dentaire et de la santé en général. répercussions cardio-vasculaires des infections parodontales

in Tempo Médical (2001), 207