References of "Rollin, Frédéric"
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See detailClinical aspects of bluetongue in ruminants
Guyot, Hugues ULg; Mauroy, Axel ULg; Kirschvink, N. et al

in Bluetongue in northern Europe (2008)

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See detailEvolution of the clinical expression of Bluetongue in Belgian cattle during year 2006 vs 2007
Guyot, Hugues ULg; Mauroy, Axel ULg; Rollin, Frédéric ULg et al

Conference (2008)

In August 2006, Belgium notified its first cases of Bluetongue (BT), serotype 8, in cattle and sheep. The disease was also observed at this time in the neighboring countries. The resurgence of BT was ... [more ▼]

In August 2006, Belgium notified its first cases of Bluetongue (BT), serotype 8, in cattle and sheep. The disease was also observed at this time in the neighboring countries. The resurgence of BT was observed in Northern Europe in 2007. The aim of the study was to compare clinical signs of BT observed in 2006 vs 2007 in Belgian cattle. The description of clinical signs was based on the observation of 38 and 39 cows in 2006 and 2007, respectively. BT cases were only included if they were confirmed by one or both laboratory diagnostic tests (competitive ELISA test and/or RT-qPCR). The inventory of clinical signs was made with a standardised clinical form for BT. This form is divided into general, cutaneous, locomotor, digestive, respiratory, neurological and reproductive clinical signs. Case data were summarised to determine changes in clinical presentation of BT between 2006 and 2007. A Fischer’s exact probability test was performed to compare (P<0.05) the frequency of clinical signs between the two years. Regarding general clinical signs, hyperthermia and tiredness were more often observed in 2007, compared to 2006. All clinical signs about skin and annexes were not significantly different between the two years. Locomotor signs such as prostration, incapacity to get up, reluctance to move, lameness and amyotrophy were more frequent in 2007. Loss of appetite, difficulties in grasping feed, salivation and drooling were the digestive signs more often observed in 2007. A purulent nasal discharge was the only respiratory sign more commonly observed in 2007. Apathy, generalised weakness and paresis or paralysis were more often encountered in 2007. The most important changes between the two years concerned reproduction. A higher incidence of abortion, premature calving and stillbirth was observed during 2007 outbreak. The frequency of most of the clinical signs of BT was higher in 2007 in Belgian cattle. Confirmed cases of BT in Belgian cattle were only 296 in 2006 compared to 4187 in 2007. These data do not represent the real situation of BT infection because the farmers do not notify all cases. Nevertheless, it seems that the 2007 outbreak was more severe regarding the number of cases and the frequency of clinical signs. The mild winter and wet 2007 summer might have favored the persistence of the vectors. [less ▲]

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See detailDevelopment and validation of a radioimmunoassay for thyrotropin in cattle
Guyot, Hugues ULg; Sulon, Joseph ULg; Beckers, Jean-François ULg et al

in Journal of Veterinary Diagnostic Investigation (2007), 19(6), 643-651

In mammals, thyrotropin, or thyroid-stimulating hormone (TSH), assay is used for the diagnosis of primary hypothyroidism. Hypothyroidism is the most common type of thyroid disorder in cattle. The aim of ... [more ▼]

In mammals, thyrotropin, or thyroid-stimulating hormone (TSH), assay is used for the diagnosis of primary hypothyroidism. Hypothyroidism is the most common type of thyroid disorder in cattle. The aim of this study was to develop and validate, under physiologic and pathologic conditions, a radioimmunoassay (RIA) for bovine TSH (bTSH). Double RIA was performed with purified bTSH and specific bovine antiserum. Laboratory validation included research of minimal detection limit, accuracy, and reproducibility. The physiologic validation included a thyrotropin-releasing hormone (TRH) challenge performed on euthyroid cows and a follow-up of bTSH concentration over a 24-hour period. Furthermore, bTSH concentration was assayed in a large population of healthy dairy and beef cows to define reference interval. The pathologic validation was made by assaying bTSH and thyroid hormones on healthy and goitrous newborn calves. The minimum detection limit (MDL) for bTSH assay was 1.3 microU/ml. The recovery was 101% to 106%. The intra- and interassay coefficients of variation (CVs) ranged from 5% to 11% and 11% to 15%, respectively. The RIA covered the whole range of physiologic bTSH values, as shown by bTSH values induced by TRH-challenge. A pulsatile secretion of bTSH was observed, accompanied by a diurnal variation with lower night values than day values. Reference intervals of bTSH ranged from 1.3 to 13.0 microU/ml for beef and dairy breeds. Finally, bTSH easily discriminated goitrous newborn calves from healthy ones, leading to the definition of a cutoff value of 35 microU/ml. The bTSH assay positively reacted to physiologic and pathologic conditions. The accuracy and precision of the RIA were satisfying. [less ▲]

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See detailMorphological alterations in oxidative muscles and mitochondrial structure associated with equine atypical myopathy
Cassart, Dominique ULg; Baise, Etienne ULg; Cherel, Yann et al

in Equine Veterinary Journal (2007), 39(1), 26-32

REASONS FOR PERFORMING STUDY: There is a lack of well documented studies about muscular lesions in equine atypical myopathy (EAM). <br /> <br />OBJECTIVES: To characterise morphopathological changes of ... [more ▼]

REASONS FOR PERFORMING STUDY: There is a lack of well documented studies about muscular lesions in equine atypical myopathy (EAM). <br /> <br />OBJECTIVES: To characterise morphopathological changes of striated muscles and myocardium, to progress understanding of this disease. <br /> <br />METHODS: Thirty-two horses age 0.5-7 years kept on pasture were referred for a sudden ataxia/myoglobinuria syndrome. Clinical examination (stiffness, muscle pain, muscle fasciculations, abnormal gait, recumbency, myoglobinuria, tachycardia, sweating) and plasma CPK, LDH and AST levels were consistent with extensive myonecrosis and, together with anamnestic data, with so-called 'equine atypical myopathy' (EAM), a disease of unknown aetiology reported since 1939. Macroscopic and microscopic (histology, histoenzymology, ultrastructure) lesions were evaluated. <br /> <br />RESULTS: Necropsic examination revealed large areas of muscle necrosis, the extent and severity of which varied between cases and muscles, but which were clearly more constant and severe in respiratory and postural muscles and in the myocardium. Histology highlighted a multifocal and monophasic process compatible with Zenker degeneration/necrosis that mostly and segmentally affected type 1 fibres. Histochemical evaluation revealed a weak and disorganised pattern of NADH tetrazolium reductase staining, the absence of calcium salts precipitates and a dramatic accumulation of lipid droplets. Ultrastructural examination often revealed fibres of which the sole modifications were altered mitochondria and sarcoplasmic lipidosis. <br /> <br />CONCLUSIONS: Taken together, the data suggest that a primary alteration of mitochondria should be considered, although secondary mitochondrial abnormalities have yet to be ruled out. <br /> <br />POTENTIAL RELEVANCE: The morphological features gathered here reveal that EAM shares most of the characteristics of toxic myopathies. [less ▲]

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See detailClinical differentiation of malignant catarrhal fever, mucosal disease and bluetongue.
Bexiga, R.; Guyot, Hugues ULg; Saegerman, Claude ULg et al

in Veterinary Record : Journal of the British Veterinary Association (2007), 161(25), 858-9

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See detailDescription clinique des cas de FCO survenus au Nord de l'Europe durant l'été et l'automne 2006
Guyot, Hugues ULg; Mauroy, Axel ULg; Thiry, Etienne ULg et al

in Bulletin des Groupements Techniques Vétérinaires (2007), 39(avril), 89-96

La fièvre catarrhale ovine (FCO) a été identifiée au Nord de l’Europe <br />le 14 août 2006. L’Allemagne, la Belgique, les Pays-Bas et, dans une <br />moindre mesure, le Grand-Duché de Luxembourg et la ... [more ▼]

La fièvre catarrhale ovine (FCO) a été identifiée au Nord de l’Europe <br />le 14 août 2006. L’Allemagne, la Belgique, les Pays-Bas et, dans une <br />moindre mesure, le Grand-Duché de Luxembourg et la France ont été <br />affectés. Le sérotype 8 du virus de la FCO (exotique) a rapidement été <br />identifié ainsi qu’un vecteur, Culicoides dewulfi, une espèce indigène du <br />Nord de l’Europe. La maladie s’est rapidement développée et au 1er <br />février 2007, on recensait 2137 foyers de FCO, toutes espèces de <br />ruminants confondues. Globalement, la maladie a affecté davantage les <br />bovins (54 %) que les ovins (46 %). Les signes cliniques les plus <br />fréquemment observés chez les bovins étaient des lésions sur le museau <br />et la cavité buccale (ulcérations/croûtes), de la salivation et des boiteries <br />chez des animaux adultes. La morbidité et la mortalité animales étaient <br />respectivement de maximum 5 % et 1 %. Les principaux signes cliniques <br />rencontrés chez le mouton étaient également des lésions sur le museau <br />et la cavité buccale (ulcérations/croûtes), de l’amaigrissement et des <br />boiteries. La morbidité et la mortalité animales étaient respectivement <br />de maximum 12 % et 6 %. Les signes cliniques n’étant pas <br />pathognomoniques et le diagnostic différentiel relativement vaste, seul <br />un examen de laboratoire permet d’établir un diagnostic de certitude. [less ▲]

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See detailThyrotropin in newborn calves as a tool for diagnosing hypothyroidism
Guyot, Hugues ULg; Lebreton, Pascal; Alves de Oliveira, Laurent et al

in Cattle Practice (2007), 15(Part 3), 271-275

The hypothalamic-pituitary-thyroid axis is of particular importance for the adaptation of mammals to their environment. Hypothyroidism is the most common type of thyroid disorders in cattle. Thyrotropin ... [more ▼]

The hypothalamic-pituitary-thyroid axis is of particular importance for the adaptation of mammals to their environment. Hypothyroidism is the most common type of thyroid disorders in cattle. Thyrotropin (TSH) is used in several mammalian species for the diagnosis of primary hypothyroidism. Currently in cattle, thyroid hormone assays are used to diagnose such disease. The aim of the study was to compare different tools for diagnosing hypothyroidism in newborn calves. Twelve goitrous newborn calves from two beef herds were studied as well as 45 healthy newborn calves. Bovine TSH, thyroxine (T4), tri-iodothyronine (T3), reverse-T3 (rT3), plasmatic inorganic iodine (PII) and glutathione peroxidase in erythrocytes (GPXe) were assayed in these calves during the first day of life. T4/T3 and T4/TSH ratio were calculated. Furthermore, TSH, T4, PII and GPXe were also assayed in their dams at calving. Results were analysed using Wilcoxon rank sum test and Spearman's rank correlation coefficient. A significant higher value (P < 0.01) of TSH was seen for the group of goitrous calves compared to healthy calves. There was no difference between healthy and goitrous calves for GPXe (P > 0.1) and PII (P > 0.05). Significantly higher values were seen for healthy calves compared to goitrous calves concerning T4, T4/T3 ratio, T4/TSH ratio, rT3 (P < 0.0 1) and T3 (P < 0.05). Regarding the group of goitrous calves, those that had larger goitre at palpation and were hairless (n=8) died within the first day of life while the four other with moderate goitre and normal hair stayed alive. Goitrous-dead calves had higher TSH values than goitrous-alive ones (P < 0.01), lower T4, T3, T4/TSH (P < 0.0 1) and rT3 (P < 0.05) but similar T4/T3 ratio (P > 0.1). Furthermore, it appeared that T4 and rT3 values in goitrous-alive calves were not different compared to healthy calves (P > 0.1). Regarding TSH (rho=0.44), T4 (rho=-0.44), PII (rho=0.70) and GPXe (rho=0.87), there was a correlation between healthy calves and their dams, with significantly higher values being found in the calves (P < 0.01). There was no correlation between values of TSH and T4 in goitrous calves and their dams (P > 0.1). However, for these animals there was a strong relationship in the values of PH (rho=0.82) and GPXe (rho=0.94) (P < 0.01). TSH, PII and GPXe were significantly higher in goitrous calves compared to their dams (P < 0.01). Regarding T4, only goitrous-dead calves showed significantly lower values than their dams (P < 0.01). Dams with goitrous calves had higher TSH (P < 0.01) and PII (P < 0.05) compared to dams with healthy calves but similar T4 and GPXe (P > 0.1). This study revealed that TSH alone is a good marker for hypothyroidism in newborn calves. In the absence of TSH assay, the T4/T3 ratio may be used to diagnose hypothyroidism in newborn calves. In our study, although T4/T3 ratio was helpful in identifying goitrous calves, it did not allow to discriminate goitrous-dead from goitrous-alive calves, contrary to TSH. In our study, the presence of a goitre in newborn calves could not be explained by the iodine (PII) and thyroid (via T4) status of their dams. [less ▲]

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See detailComparative responses to sodium selenite and organic selenium supplements in Belgian Blue cows and calves
Guyot, Hugues ULg; Spring, P.; Andrieu, S. et al

in Livestock Science (2007), 111(3), 259-263

Belgian Blue (BB) beef cattle is particularly prone to selenium (Se) deficiency due to the poor Se content of soil and roughages on rearing farms and the higher requirements of this hypermuscled breed ... [more ▼]

Belgian Blue (BB) beef cattle is particularly prone to selenium (Se) deficiency due to the poor Se content of soil and roughages on rearing farms and the higher requirements of this hypermuscled breed. The goal of this trial was to compare the effects of different forms and concentrations of Se supplementation on Se status, health and performance in 60 pregnant Se-deficient BB cows. Cows were allocated to 3 experimental groups receiving selenized-yeast at 0.5 ppm Se on total ration (Y-Se 0.5), Na-selenite at 0.5 ppm Se on total ration (Na-Se 0.5) and Na-selenite at 0.1 ppm Se on total ration (Na-Se 0.1), respectively. Cows were supplemented from 2 months before calving until 2 months after calving. Data on performance, health and Se status of the dams and their calves were analyzed using a linear model, least squares means and logistic regression. At the end of the study, plasmatic Se (pSe) was significantly higher (P<0.01) in cows receiving Y-Se than in cows from other groups. Glutathion-peroxidase in erythrocytes (GSH-pxe) was higher in Y-Se and Na-Se 0.5 than Na-Se 0.1 group (P<0.01). Se content in colostrum and milk was significantly higher (P< 0.01) in Y-Se than other groups. At birth, Se status of calves from group Y-Se was significantly higher than those of other groups (P<0.01). Plasmatic Se in calves remained higher for 75 days after birth in Y-Se compared to other groups (P<0.01). Diarrhoea was the most commonly observed disease in the calves and, during the first 15 days of life, diarrhoea occurred in 6%, 21% and 35% of calves from groups Y-Se, Na-Se 0.5 and Na-Se 0.1, respectively. Over the whole 75 days trial period, incidence of diarrhoea was 19, 29 and 65%, respectively. Average daily gain (ADG) in calves born from Y-Se group of cows tended to be higher than in Na-Se 0.5 (P=0.06) and Na-Se 0.1 (P<0.05) but there was no difference between Na-Se 0.5 and Na-Se 0.1 (P>0.1). At the same dosage, Y-Se conferred better Se status in both dams and their calves than did Na-Se. Requirement of 0.1 ppm Se seems to be insufficient in BB to optimise health and performance. Regarding health status and ADG in calves, Y-Se seems also to result in better performance. (C) 2007 Elsevier B.V. All rights reserved. [less ▲]

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See detailLe diagnostic des carences en Iode et Sélénium chez les bovins
Guyot, Hugues ULg; Rollin, Frédéric ULg

in Annales de Médecine Vétérinaire (2007), 151

Selenium and iodine deficiencies are frequently present in cattle in Europe with a negative impact on animal health, reproduction and production. Deficiencies in animals also influence human health owing ... [more ▼]

Selenium and iodine deficiencies are frequently present in cattle in Europe with a negative impact on animal health, reproduction and production. Deficiencies in animals also influence human health owing to consumption of their products. These deficiencies must be correctly diagnosed in order to avoid lack of diagnosis or wrong diagnosis. Since there are many interactions between nutrients, a nutritional approach only is of limited interest. Therefore, the diagnosis based on samples obtained on the animals is more reliable. Clinical signs of selenium and iodine deficiencies are rarely pathognomonic. Laboratory analyses are thus necessary to confirm the diagnosis. The present review relates the principal clinical signs of selenium and iodine deficiencies and the confirmation of the deficiency by the measurements of different nutritional and functional markers available for cattle, either on blood, milk, urine or tissues. Finally, a protocol for the diagnosis of selenium and iodine deficiencies is proposed [less ▲]

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See detailRespiratory distress syndrome in full-term newborn calves
Rollin, Frédéric ULg; Danlois, Fabien; Aliaoui, Hamani et al

Conference (2007)

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See detailFièvre Catarrhale Ovine chez les ruminants. Description clinique des cas vécus dans le Nord de l’Europe durant l’été-automne 2006
Guyot, Hugues ULg; Mauroy, Axel ULg; Thiry, Etienne ULg et al

in Bulletin des GTV (2007)

La fièvre catarrhale ovine (FCO) a été identifiée au Nord de l’Europe le 14 août 2006. L’Allemagne, la Belgique, les Pays-Bas et, dans une moindre mesure, le Grand-Duché de Luxembourg et la France ont été ... [more ▼]

La fièvre catarrhale ovine (FCO) a été identifiée au Nord de l’Europe le 14 août 2006. L’Allemagne, la Belgique, les Pays-Bas et, dans une moindre mesure, le Grand-Duché de Luxembourg et la France ont été affectés. Le sérotype 8 du virus de la FCO (exotique) a rapidement été identifié ainsi qu’un vecteur, Culicoides dewulfi, une espèce indigène du Nord de l’Europe. La maladie s’est rapidement développée et au 1er février 2007, on recensait 2137 foyers de FCO, toutes espèces de ruminants confondues. Globalement, la maladie a affecté davantage les bovins (54 %) que les ovins (46 %). Les signes cliniques le plus fréquemment observés chez les bovins étaient des lésions sur le museau et la cavité buccale (ulcérations/croûtes), de la salivation et des boiteries chez des animaux adultes. La morbidité et la mortalité animales étaient respectivement de maximum 5 % et 1 %. Les principaux signes cliniques rencontrés chez le mouton étaient également des lésions sur le museau et la cavité buccale (ulcérations/croûtes), de l’amaigrissement et des boiteries. La morbidité et la mortalité animales étaient respectivement de maximum 12 % et 6 %. Les signes cliniques n’étant pas pathognomoniques et le diagnostic différentiel relativement vaste, seul un examen de laboratoire permet de poser un diagnostic de certitude. [less ▲]

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See detailCongenital muscular dystonia (CMD): a new congenital pathology in Belgian Blue calves
Vandeputte, Sébastien ULg; Brihoum, Mounir ULg; Hubin, Xavier et al

Conference (2006, October 15)

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See detailFully automated heart rate variability assessment in dairy cows
Janda, Jozef; Guyot, Hugues ULg; Sandersen, Charlotte ULg et al

in Acta Physiologica (2006, May), 187(Suppl 651), -16

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See detailCombined spastic paresis of both gastrocnemius and femoral quadriceps muscles in Belgian blue calves
Janda, Jozef; Uyttenhoef, Aude; Vandeputte, Sébastien ULg et al

Conference (2006)

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See detailEvaluation of systolic function in calves by Doppler echocardiography: preliminary results
Brihoum, M; Rollin, Frédéric ULg; Desmecht, Daniel ULg et al

in Proceedings of the XXIVth World Buiatrics Congress (2006)

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