References of "Rogister, Bernard"
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See detailEffects of eccentric versus concentric training on mouse muscle phenotype
Hody, Stéphanie ULg; Lacrosse, Zoé ULg; Leprince, Pierre ULg et al

in Abstract Book of 10th Meeting of Belgian Society for Neuroscience (2013, May)

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See detailAdult Mesenchymal Stem Cells, Adult Neural Crest Stem Cells and Therapy of Neurological Pathologies: a State of Play
Neirinckx, Virginie ULg; Coste, Cécile ULg; Rogister, Bernard ULg et al

in Stem Cells Translational Medicine (2013), 2(4), 284-296

Adult stem cells are endowed with in vitro multi-lineage differentiation abilities, and constitute an attractive autologous source of material for cell therapy in neurological disorders. With regards to ... [more ▼]

Adult stem cells are endowed with in vitro multi-lineage differentiation abilities, and constitute an attractive autologous source of material for cell therapy in neurological disorders. With regards to lately published results, the ability of adult mesenchymal stem cells (MSC) and neural crest stem cells (NCSC) to integrate and differentiate into neurons once inside the central nervous system (CNS) is currently questioned. In this review, we collected exhaustive data on MSC/NCSC neural differentiation in vitro. We then analyzed pre-clinical cell therapy experiments in different models for neurological diseases and concluded that neural differentiation is probably not the leading property of adult MSC and NCSC concerning neurological pathologies management. Definitely, a fine analysis of the molecules that are secreted by MSC and NCSC would be of significant interest regarding their important contribution to the clinical and pathological recovery after CNS lesions. [less ▲]

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See detailNeural fate of Mesenchymal Stem Cells and Neural Crest Stem Cells : Which ways to get neurons for cell therapy purpose ?
Neirinckx, Virginie ULg; Coste, Cécile ULg; Rogister, Bernard ULg et al

in Trends in cell signaling pathways in neuronal fate decision (2013)

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See detailPrévention des courbatures musculaires en pratique sportive
Hody, Stéphanie ULg; Delvaux, François; Rodriguez de la Cruz, Carlos ULg et al

in Prévention des lésions musculo-squelettiques chez le sportif (2013)

L’exercice excentrique, lorsqu’il est réalisé de manière intense et/ou inhabituelle, est réputé être à l’origine de diverses sensations de courbatures d’apparition retardée. Ces douleurs musculaires ... [more ▼]

L’exercice excentrique, lorsqu’il est réalisé de manière intense et/ou inhabituelle, est réputé être à l’origine de diverses sensations de courbatures d’apparition retardée. Ces douleurs musculaires, appelées DOMS pour Delayed-Onset Muscle Soreness, s’accompagnent de déficits structuro-fonctionnels pouvant persister pendant plusieurs jours. Bien qu’il disparaisse spontanément après quelques jours de récupération, le phénomène des DOMS reste problématique chez le sportif en raison des conséquences néfastes associées : perturbation de l’entraînement sportif, diminution de la performance athlétique et majoration du risque de lésions véritables en cas de poursuite d’activités sportives. Il apparaît donc essentiel, pour un sportif soumis à un entraînement excentrique, d’échapper aux DOMS. La mise au point d’approches susceptibles de réduire les conséquences néfastes des DOMS a constitué un objectif prioritaire de nombreuses études. Ainsi, les premiers essais ont été basés sur des stratégies nutritionnelles ou pharmacologiques ainsi que sur des procédés cliniques. Bien que certains procédés tels que le massage ou les apports nutritionnels puissent influencer de manière positive l’un ou l’autre symptôme, ceux-ci ne semblent pas capables d’agir sur le phénomène des DOMS dans sa globalité. Actuellement, la seule démarche préventive réellement efficace reste la réalisation d’un entraînement excentrique à intensité sous-maximale progressivement intensifiées. [less ▲]

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See detail4-Bromo-2-(piperidin-1-yl)thiazol-5-yl-phenyl methanone (12b) Inhibits Na+/K+-ATPase and Ras Oncogene Activity in Cancer Cells
Lefranc, F; Xu, Z; Burth, P et al

in European Journal of Medicinal Chemistry (2013), 63

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See detailIn Vivo Tumorigenesis Was Observed after Injection of In Vitro Expanded Neural Crest Stem Cells Isolated from Adult Bone Marrow
Wislet, Sabine ULg; Poulet, Christophe ULg; Neirinckx, Virginie ULg et al

in PLoS ONE (2012), 7(10), 46425

Bone marrow stromal cells are adult multipotent cells that represent an attractive tool in cellular therapy strategies. Several studies have reported that in vitro passaging of mesenchymal stem cells ... [more ▼]

Bone marrow stromal cells are adult multipotent cells that represent an attractive tool in cellular therapy strategies. Several studies have reported that in vitro passaging of mesenchymal stem cells alters the functional and biological properties of those cells, leading to the accumulation of genetic aberrations. Recent studies described bone marrow stromal cells (BMSC) as mixed populations of cells including mesenchymal (MSC) and neural crest stem cells (NCSC). Here, we report the transformation of NCSC into tumorigenic cells, after in vitro long-term passaging. Indeed, the characterization of 6 neural crest-derived clones revealed the presence of one tumorigenic clone. Transcriptomic analyses of this clone highlighted, among others, numerous cell cycle checkpoint modifications and chromosome 11q down-regulation (suggesting a deletion of chromosome 11q) compared with the other clones. Moreover, unsupervised analysis such as a dendrogram generated after agglomerative hierarchical clustering comparing several transcriptomic data showed important similarities between the tumorigenic neural crest-derived clone and mammary tumor cell lines. Altogether, it appeared that NCSC isolated from adult bone marrow represents a potential danger for cellular therapy, and consequently, we recommend that phenotypic, functional and genetic assays should be performed on bone marrow mesenchymal and neural crest stem cells before in vivo use, to demonstrate whether their biological properties, after ex vivo expansion, remain suitable for clinical application. [less ▲]

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See detailMesenchymal stem cells and neural crest stem cells from adult bone marrow: characterization of their surprising similarities and differences.
Wislet, Sabine ULg; Laudet, Emerence ULg; Neirinckx, Virginie ULg et al

in Cellular and Molecular Life Sciences : CMLS (2012), 69(15), 2593-2608

The generation of neuronal cells from stem cells obtained from adult bone marrow is of significant clinical interest in order to design new cell therapy protocols for several neurological disorders. The ... [more ▼]

The generation of neuronal cells from stem cells obtained from adult bone marrow is of significant clinical interest in order to design new cell therapy protocols for several neurological disorders. The recent identification in adult bone marrow of stem cells derived from the neural crest stem cells (NCSC) might explain the neuronal phenotypic plasticity shown by bone marrow cells. However, little information is available about the nature of these cells compared to mesenchymal stem cells (MSC), including their similarities and differences. In this paper, using transcriptomic as well as proteomic technologies, we compared NCSC to MSC and stromal nestin-positive cells, all of them isolated from adult bone marrow. We demonstrated that the nestin-positive cell population, which was the first to be described as able to differentiate into functional neurons, was a mixed population of NCSC and MSC. More interestingly, we demonstrated that MSC shared with NCSC the same ability to truly differentiate into Tuj1-positive cells when co-cultivated with paraformaldehyde-fixed cerebellar granule neurons. Altogether, those results suggest that both NCSC and MSC can be considered as important tools for cellular therapies in order to replace neurons in various neurological diseases. [less ▲]

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See detailProteomic comparison of high and low responders to eccentric exercise
Hody, Stéphanie ULg; Croisier, Jean-Louis ULg; Leprince, Pierre ULg et al

in Meeusen, R.; Duchateau, J.; Roelands, B. (Eds.) et al Book of Abstracts of the 17th annual Congress of the ECSS (2012, July)

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See detailMuscle fatigue experienced during maximal eccentric exercise is predictive of the plasma creatine kinase (CK) response
Hody, Stéphanie ULg; Rogister, Bernard ULg; Leprince, Pierre ULg et al

in Meeusen, R.; Duchateau, J.; Roelands, B. (Eds.) et al Book of Abstracts of the 17th annual Congress of the ECSS (2012, July)

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See detailValidation of Adult Bone Marrow Stromal Cells in Cellular Therapy Protocols, using a Mouse Model for Parkinson’s Disease
Neirinckx, Virginie ULg; Laudet, Emerence; Rogister, Bernard ULg et al

Poster (2012, May 04)

Parkinson’s disease (PD) is the second most common neurodegenerative disorder in industrialized countries. Its main characteristic relies in a progressive loss of dopaminergic (DA) neurons in the ... [more ▼]

Parkinson’s disease (PD) is the second most common neurodegenerative disorder in industrialized countries. Its main characteristic relies in a progressive loss of dopaminergic (DA) neurons in the Substantia Nigra pars compacta (SNpc), resulting in a deficient dopamine release in the striatum and then promoting important defects in motility regulation. Unfortunately, motor symptoms are generally diagnosed once 80% of nigrostriatal neurons are already lost. The emergence of neuroprotective/-restorative strategies is then increasingly raising hope, and a lot of people now focus on cell therapy experiments. Adult bone marrow stromal stem cells (BMSCs) have already been demonstrated as ideal candidates for cell therapy in nervous lesions, regarding their high multipotency and the fact they can be easily harvested in the patient himself. After it has been demonstrated that some BMSCs arise from the embryonic neural crest (NC), we compared NC-BMSCs and mesenchymal (M)-BMSCs in vitro, in terms of differenciation abilities and more particularly in terms of neural fate. We then wanted to investigate and compare the potential usefulness of both populations in the context of a neurological pathology. We have validated a MPTP mouse model, mimicking the specific loss of nigral neurons, and started setting up a cell therapy experiment using stereotaxic brain injection of the two types of BMSCs. The survival rate of grafted cells was analyzed as well as their migration or differentiation, and their ability to restore neuronal loss was also observed. Our first results showed that once grafted inside the brain of MPTP mice, NC-BMSCs survive for about a week, staying tightly close to each other the injection track with no visible sign of migration. Afterwards, cells begin to disappear and we only observe a mean survival rate of 1% after 28 days. Looking at the nigrostriatal pathway integrity, neural crest-BMSCs don’t seem to induce any improvement: they don’t differenciate into neural cells, neither replace lost DA cells, and they do not induce any sprouting of surviving DA neurons. While the M-BMSCs graft experiment has to be completed, these first results showed that NC-BMSCs at the stem cell state are not able to restore the lesioned system, and maybe a pre-differenciation step would be required to trigger those cells into a neuronal fate before grafting them in a MPTP-mouse brain. [less ▲]

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See detailAltered balance between excitatory and inhibitory inputs onto CA1 pyramidal neurons from SV2A-deficient but not SV2B-deficient mice.
Venkatesan, Kumar; Alix, Philippe ULg; Marquet, Alice et al

in Journal of Neuroscience Research (2012), 90(12), 2317-27

Synaptic vesicle protein 2 (SV2) is a glycoprotein that exists in three isoforms, SV2A, SV2B, and SV2C. SV2A knockout (KO) mice and SV2A/SV2B double KO (DKO) mice, but not SV2B KO animals, start to ... [more ▼]

Synaptic vesicle protein 2 (SV2) is a glycoprotein that exists in three isoforms, SV2A, SV2B, and SV2C. SV2A knockout (KO) mice and SV2A/SV2B double KO (DKO) mice, but not SV2B KO animals, start to experience severe seizures and weight loss 7 days after birth and die at about postnatal day (P)14-P23. Because excitatory and inhibitory inputs play a major role in controlling neuronal excitability in the hippocampus, we examined the effects of SV2A and/or SV2B deletions on glutamatergic and GABA(A) neurotransmission in hippocampal CA1 pyramidal neurons. Spontaneous and miniature excitatory and inhibitory postsynaptic currents (sEPSCs, mEPSCs, sIPSCs, and mIPSCs, respectively) were recorded using the whole-cell patch-clamp technique in slices from P6-P14 mice. The frequency of sEPSCs was increased in SV2A KO and SV2A/SV2B DKO mice, but their amplitude was unchanged. Such changes were not observed in SV2B KOs. On the contrary, the frequency and amplitude of sIPSCs were decreased in SV2A KO and SV2A/SV2B DKO mice but not in SV2B KO animals, as reported previously for the CA3 region. Kinetic parameters of sIPSCs and sEPSCs were unchanged. Importantly, no changes were observed in any genotype when examining mEPSCs and mIPSCs. We conclude that action potential- and Ca(2+) -dependent glutamatergic and GABAergic synaptic transmission are differentially altered in the hippocampus of SV2A-deficient mice, whereas the mechanism of exocytosis itself is not changed. The altered balance between these major excitatory and inhibitory inputs is probably a contributing factor to seizures in SV2A KO and SV2A/SV2B DKO mice. (c) 2012 Wiley Periodicals, Inc. [less ▲]

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See detailAdult neural crest stem cells and neuronal fate decision
Neirinckx, Virginie ULg; Rogister, Bernard ULg; Wislet, Sabine ULg

in Trends in cell signaling pathways in neuronal fate decision (2012)

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See detailN-Aryl-N'-(chroman-4-yl)ureas and thioureas display in vitro anticancer activity and selectivity on apoptosis-resistant glioblastoma cells: screening, synthesis of simplified derivatives, and structure-activity relationship analysis.
Goffin, Eric ULg; Lamoral-Theys, Delphine; Tajeddine, Nicolas et al

in European Journal of Medicinal Chemistry (2012), 54

A series of chroman derivatives previously reported as potassium channel openers, as well as some newly synthesized simplified structures, were examined for their in vitro effects on the growth of three ... [more ▼]

A series of chroman derivatives previously reported as potassium channel openers, as well as some newly synthesized simplified structures, were examined for their in vitro effects on the growth of three human high-grade glioma cell lines: U373, T98G, and Hs683. Significant in vitro growth inhibitory activity was observed with 2,2-dimethylchroman-type nitro-substituted phenylthioureas, such as compounds 4o and 4p. Interestingly, most tested phenylureas were found to be slightly less active, but more cell selective (normal versus tumor glial cells, such as 3d, 3e, and 3g), thus less toxic, than the corresponding phenylthioureas. No significant differences were observed in terms of chroman-derivative-induced growth inhibitory effects between glioma cells sensitive to pro-apoptotic stimuli (Hs683 glioma cells) and glioma cells associated with various levels of resistance to pro-apoptotic stimuli (U373 and T98G glioma cells), a feature that suggests non-apoptotic-mediated growth inhibition. Flow cytometry analyses confirmed the absence of pro-apoptotic effects for phenylthioureas and phenylureas when analyzed in U373 glioma cells and demonstrated U373 cell cycle arrest in the G0/G1 phase. Computer-assisted phase-contrast videomicroscopy revealed that 3d and 3g displayed cytostatic effects, while 3e displayed cytotoxic ones. As a result, this work identified phenylurea-type 2,2-dimethylchromans as a new class of antitumor agents to be further explored for an innovative therapeutic approach for high-grade glioma and/or for a possible new mechanism of action. [less ▲]

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See detailHuman muscle proteome modifications after acute or repeated eccentric exercises
Hody, Stéphanie ULg; Leprince, Pierre ULg; Sergeant, K. et al

in Medicine & Science in Sports & Exercise (2011), 43(12), 2281-2296

INTRODUCTION:: DOMS (Delayed-Onset Muscle Soreness), a condition triggered by eccentric exercise, affects muscle cells at a biochemical level in a poorly understood fashion. The objective of the present ... [more ▼]

INTRODUCTION:: DOMS (Delayed-Onset Muscle Soreness), a condition triggered by eccentric exercise, affects muscle cells at a biochemical level in a poorly understood fashion. The objective of the present study was to examine human muscle proteome modifications induced by strenuous eccentric exercises following a specific training aimed to prevent DOMS. METHODS:: Biopsy of the rectus femoris were taken from healthy human volunteers in three successive conditions: (1) at rest, (2) 24 hours after an injuring exercise protocol consisting of 3 series of 30 maximal contractions of the quadriceps on an isokinetic dynamometer, (3) 24 hours after a similar exercise bout preceded either by 5 eccentric training sessions, or no training. RESULTS:: Muscle damage was assessed before and 1 day after each maximal eccentric test by comparing three indirect markers: plasma activity of creatine kinase (CK), muscle stiffness and subjective pain intensity. Compared to the first eccentric test, those markers were reduced after the second test and further reduced if this second test followed the eccentric training, thus confirming the protective effect of such training. Muscle protein extracts were subjected to a 2D-DIGE proteomic analysis coupled with MALDI-TOF-MS protein identification. Surprisingly, we observed that myosin heavy chains decreased after the first eccentric test, and were reduced further with other contractile proteins after the second test. Furthermore, the expression of several glycolytic enzymes decreased only after the second test that was preceded by a specific training. CONCLUSION:: These findings suggest that the eccentric training resulted in a switch to oxidative metabolism, which may be associated with protection from DOMS. [less ▲]

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See detailAdult bone marrow: which stem cells for cellular therapy protocols in neurodegenerative disorders?
Wislet-Gendebien, Sabine ULg; Laudet, Emerence ULg; Neirinckx, Virginie ULg et al

in Journal of Biomedicine & Biotechnology (2011)

The generation of neuronal cells from stem cells obtained from adult bone marrow is of significant clinical interest in order to design new cell therapy protocols for several neurological disorders. The ... [more ▼]

The generation of neuronal cells from stem cells obtained from adult bone marrow is of significant clinical interest in order to design new cell therapy protocols for several neurological disorders. The recent identification in adult bone marrow of stem cells derived from the neural crests (NCSC) might explain the neuronal phenotypic plasticity shown by bone marrow cells. However, little information is available about the nature of these cells compared to mesenchymal stem cells (MSC). In this manuscript, we will review all information available concerning NCSC from adult tissues and their possible use in regenerative medecine. Moreover, as multiple recent studies showed the beneficial effect of bone marrow stromal cells in neurodegenerative diseases, we will discuss which stem cells isolated from adult bone marrow should be more suitable for cell replacement therapy. [less ▲]

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See detailWnt1 and BMP2: two factors recruiting multipotent neural crest progenitors isolated from adult bone marrow
Glejzer, Aneta ULg; Laudet, Emerence ULg; Leprince, Pierre ULg et al

in Cellular and Molecular Life Sciences : CMLS (2011), 68/12

Recent studies have shown that neural crestderived progenitor cells can be found in diverse mammalian tissues including tissues that were not previously shown to contain neural crest derivatives, such as ... [more ▼]

Recent studies have shown that neural crestderived progenitor cells can be found in diverse mammalian tissues including tissues that were not previously shown to contain neural crest derivatives, such as bone marrow. The identification of those ‘‘new’’ neural crest-derived progenitor cells opens new strategies for developing autologous cell replacement therapies in regenerative medicine. However, their potential use is still a challenge as only few neural crest-derived progenitor cells were found in those new accessible locations. In this study, we developed a protocol, based on wnt1 and BMP2 effects, to enrich neural crest-derived cells from adult bone marrow. Those two factors are known to maintain and stimulate the proliferation of embryonic neural crest stem cells, however, their effects have never been characterized on neural crest cells isolated from adult tissues. Using multiple strategies from microarray to 2D-DIGE proteomic analyses, we characterized those recruited neural crest-derived cells, defining their identity and their differentiating abilities. [less ▲]

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