References of "Rentier, Bernard"
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See detailComparison of conjugation procedures for the preparation of monoclonal antibody-enzyme conjugates
Jeanson, Antoinette; Cloes, Jean-Michel; Bouchet, Mireille et al

in Journal of Immunological Methods (1988), 111(2), 261-270

Four monoclonal antibodies belonging to different subclasses and with differing isoelectric points were coupled to horseradish peroxidase (HRP) and alkaline phosphatase (AP) using various conjugation ... [more ▼]

Four monoclonal antibodies belonging to different subclasses and with differing isoelectric points were coupled to horseradish peroxidase (HRP) and alkaline phosphatase (AP) using various conjugation procedures. The conjugates were tested by enzyme immunoassay and their efficiency was characterized by the antibody and enzyme concentrations needed to obtain an arbitrary OD value. The suitability of antibody for conjugation through NH2 groups was tested by fluorodinitrobenzene (FDNB). HRP conjugates were produced by two variants of the sodium periodate procedure and two variants of the glutaraldehyde method, as well as by the heterobifunctional linker N-succinimidyl 3-(2-pyridyldithio)pro-pionate (SPDP). Two of the four antibodies were coupled by a third variant of the periodate method, through their carbohydrate moieties. The periodate-mediated conjugations, using sugar moieties on the enzyme, provided the most efficient HRP conjugates, regardless of the antibody subclass or isoelectric point. The glutaraldehyde procedures consistently gave the worst results. AP conjugates were prepared using the same methods. The most efficient and reproducible AP conjugates with all four monoclonal antibodies were obtained using the SPDP procedure. The efficiency of the other methods differed from one antibody to another. [less ▲]

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See detailElucidation of non-parallel EIA curves
François-Gérard, C.; Gérard, Paul ULg; Rentier, Bernard ULg

in Journal of Immunological Methods (1988), 111(1), 59-65

Quantitative determinations by EIA can be only obtained by reverse regression when linear portions of sample and standard curves are parallel. However, analysis of complex biological fluids often yields ... [more ▼]

Quantitative determinations by EIA can be only obtained by reverse regression when linear portions of sample and standard curves are parallel. However, analysis of complex biological fluids often yields sigmoid curves displaying lower slopes, thus invalidating any quantitative interpretation. We hypothesized that this phenomenon was due to a competition effect between the target (for example an antigen) and related molecules for the binding sites (for example a capture antibody) immobilized onto the solid phase. This has been confirmed experimentally using various target-to-competitor ratios and formulated as a mathematical model. The slope decrease in target detection was related to the proportion of competitor, not in a linear, but in an exponential manner. This mathematical model has been computerized and can be used to correct aberrant sample curves provided the relevant parameters have been previously determined in the same systems. A competition effect should be suspected whenever non-parallel EIA sigmoid slopes are obtained. [less ▲]

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See detailMonoclonal antibody from hybrid hybridoma: two specificities in one molecule
Rentier, Bernard ULg; Cloes, Jean-Michel

Conference (1988)

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See detailInterprétation du Western Blot (WB)
François-Gérard, C.; Vaira, Dolorès ULg; Rentier, Bernard ULg et al

Conference (1988)

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See detailSet up of an easy selective method for the isolation of hybrid hybridomas. Evidence for the production of heterobifunctional monoclonal antibodies
Cloes, Jean-Michel; Calberg-Bacq, Claire Michelle; François, C. et al

in Archives Internationales de Physiologie, de Biochimie et de Biophysique (1988), 96

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See detailPersistent varicella-zoster virus infection in the nervous system: in vitro and in vivo models
Sadzot-Delvaux, Catherine ULg; Merville, Marie-Paule ULg; Bourdon-Wouters, C. et al

in Acta Neurologica Belgica (1988), 88

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See detailIn vitro neuroblastoma cell infection by varicella-zoster virus
Bourdon-Wouters, C.; Merville, Marie-Paule ULg; Piette, Jacques ULg et al

Poster (1988)

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See detailLe système immunitaire : principes généraux
Rentier, Bernard ULg

in Journal de Pharmacie de Belgique (1988), 43(3), 185-211

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See detailIn vitro and in vivo models of varicella-zoster virus persistence in the nervous system
Sadzot-Delvaux, Catherine ULg; Merville, Marie-Paule ULg; Bourdon-Wouters, C. et al

in Journal of Cellular Biochemistry (1988), 12C

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See detailProduction of heterobispecific monoclonal antibodies by mouse hybrid hybridomas (quadromas)
Cloes, Jean-Michel; Herens, Christian ULg; Nys, Monique ULg et al

in Archives Internationales de Physiologie, de Biochimie et de Biophysique (1988), 96

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See detailDetection of cytomegalovirus with labeled DNA probes
Bourdon-Wouters, C.; Sadzot-Delvaux, Catherine ULg; Merville, Marie-Paule ULg et al

Poster (1987)

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See detailMolecular cloning of varicella-zoster virus DNA and its detection in situ in infected nerve cells
Merville, Marie-Paule ULg; Sadzot-Delvaux, Catherine ULg; Delrée, P. et al

in Archives Internationales de Physiologie et de Biochimie (1987), 95(2), 31

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See detailDetection and identification of the cytomegalovirus genome in infected cells with labeled DNA probes
Bourdon-Wouters, Christine; Merville, Marie-Paule ULg; Sadzot-Delvaux, Catherine ULg et al

in Archives Internationales de Physiologie et de Biochimie (1987)

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See detailProblems in quantitation of anti-viral IgG isotypes: a computerized mathematical model
Rentier, Bernard ULg; Francois-Gerard, C.; Gérard, Paul ULg

in Charbonneau, K. (Ed.) Abstracts : sixth international congress of immunology, July 6-11,1986, Toronto, Canada (1986)

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