References of "Rentier, Bernard"
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See detailParasite communities: Patterns and Processes
Rentier, Bernard ULg

in Book Reviews, Biochemical Systematics and Ecology (G. Esch, A. Bush & J. Aho, eds., Chapman & Hall, 1990) (1991)

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See detailAn in vivo model of varicella-zoster virus latent infection of dorsal root ganglia
Sadzot-Delvaux, Catherine ULg; Merville, Marie-Paule ULg; Delrée, P. et al

in Journal of Neuroscience Research (1990), 26(1), 83-89

We describe here the first in vivo model of varicella-zoster virus (VZV) latent infection in the adult rat peripheral nervous system. Infected Mewo cells were injected subcutaneously along the spine of ... [more ▼]

We describe here the first in vivo model of varicella-zoster virus (VZV) latent infection in the adult rat peripheral nervous system. Infected Mewo cells were injected subcutaneously along the spine of healthy adult rats. No clinical sign of infection was observed even 9 months after inoculation. Humoral immune response to VZV was detected in all infected animals throughout the study (9 months). The presence of viral material in dissociated and cultured dorsal root ganglia (DRG) from inoculated animals was studied by immunoperoxidase and in situ hybridization. When DRGs from infected animals were plated in culture from 1 month and up to 9 months after inoculation, viral nucleic acids and proteins were detected in neurons. Furthermore, trypsinization and subcultivation of infected neurons in culture is needed to reactivate infectious virus at least in some of the neurons. This model provides a useful tool for studying 1) the molecular mechanisms leading to an in vivo latency, 2) the role of the immune system, in particular cellular immunity, on the establishment, maintenance, and reactivation of latency, 3) the neurotropism of mutant viruses, and 4) the effects of antiviral agents. [less ▲]

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See detailAcute and persistent varicella-zoster virus infection of human and murine neuroblastoma cell lines
Bourdon-Wouters, C.; Merville, Marie-Paule ULg; Sadzot-Delvaux, Catherine ULg et al

in Journal of Neuroscience Research (1990), 26(1), 90-97

Human and murine neuroblastoma cell lines were infected in vitro with varicella-zoster virus (VZV). Infected human neuroblastoma cells (IMR-32) supported the synthesis of abundant viral antigens as ... [more ▼]

Human and murine neuroblastoma cell lines were infected in vitro with varicella-zoster virus (VZV). Infected human neuroblastoma cells (IMR-32) supported the synthesis of abundant viral antigens as detected by indirect immunoperoxidase labeling using human serum rich in anti-VZV antibodies and did not survive the infection. In situ hybridization (ISH) with VZV-cloned probes revealed a strong hybridization signal in these infected cells. During cultivation, the virus was released in the culture medium, and viral polypeptides were revealed by Western blotting of infected cells, using either a monoclonal anti-gpI antibody or a rabbit antiserum. All these findings indicate that IMR-32 cells support a productive and lytic infection by VZV, whether infected by cell-free virus or by cocultivation with infected cells. Murine neuroblastoma cells (neuro-2A) survived VZV infection and did not produce any infectious virus. No VZV-specific proteins were detected in infected cells either by immunolabeling or by Western blotting. However, viral nucleic acids could be detected by ISH, indicating that mouse neuroblastoma cells displayed a nonproductive, nonlytic infection. Infected neuro-2A cells have been examined by ISH using probes corresponding to immediate early (IE) genes 4, 62, and 63 and late (L) gene 31 encoding gpII. A strong hybridization signal was detected when infected cells were probed with a fragment containing the IE genes 62 and 63. Lower levels of hybridization were detected with the other probes, corresponding to IE or L genes. These systems allow comparative molecular analysis of persistent and acute infection of nerve cells by VZV. [less ▲]

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See detailTyping of human papillomaviruses in genital specimens by DNA hybridization
Lauricella, M.-A.; Piette, Jacques ULg; Lifrange, Eric ULg et al

in Archives Internationales de Physiologie et de Biochimie (1990), 98(2), 34

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See detailReactivation of HIV-1 after an oxidative stress
Piette, Jacques ULg; Legrand-Poels, Sylvie; Vaira, Dolorès ULg et al

in Free Radicals in Biology and Medicine (1990), 9(Suppl. A), 5

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See detailDiagnosis by PCR of HIV-1 infection in seronegative individuals at risk
Vaira, Dolorès ULg; François-Gérard, Ch.; Doppagne, A. et al

in AIDS Research and Human Retroviruses (1990), 6(2), 173-174

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See detailImmunological Techniques in Insect Biology (L.I. Gilbert & T.A. Miler, eds.)
Rentier, Bernard ULg

in Biochemical Systematics & Ecology (1989), 17(6), 501-502

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See detailMéthodes de détection de l'infection par le virus de l'immunodéficience humaine (HIV)
François-Gérard, Ch.; Warling, Ch.; Vaira, Dolorès ULg et al

in Revue Médicale de Liège (1989), 44(8), 295-305

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See detailIntérêt du typage viral des papillomavirus humains (HPV) en relation avec les neoplasies des muqueuses genitales
Lifrange, Eric ULg; Lauricella, M.; Rentier, Bernard ULg et al

in Revue Médicale de Liège (1989), 44(1), 1-14

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See detailDiagnosis of HIV-1 in African couples : comparison of serology and PCR
Vaira, Dolorès ULg; Sondag, Danièle ULg; François-Gérard, C. et al

in Cinquième conférence internationale sur le SIDA : le défi scientifique et social (1989)

OBJECTIVE: Search for the rate of HIV-1 contamination among seronegative sexual partners of seropositive individuals. METHODS: Classical serological methods (EIA, WB) and PCR. SERIES: 36 heterosexual ... [more ▼]

OBJECTIVE: Search for the rate of HIV-1 contamination among seronegative sexual partners of seropositive individuals. METHODS: Classical serological methods (EIA, WB) and PCR. SERIES: 36 heterosexual couples from central Africa, accounting for a total of 73 persons: 13/37 seropositive women, 23/36 seropositive men. All couples were serologically discordant, i.e. one partner was seropositive. CONCLUSIONS: In such a population, particularly at risk of HIV contamination, the rate of false negative serological diagnosis reached 70%. [less ▲]

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See detailDiagnostic des infections génitales à Papillomavirus
Lifrange, Eric ULg; Rentier, Bernard ULg

Conference (1989)

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See detailAbsence of seroconversion in a PCR positive person 18 months after transfusion of HIV infected blood
Vaira, Dolorès ULg; François-Gérard, C.; Rentier, Bernard ULg et al

in Vox Sanguinis (1989), 57(3), 220-221

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See detailEvaluation d'une nouvelle méthode pour le dépistage précoce du cancer colo-rectal
Rentier, Bernard ULg; Collin-Marcq, O.

in Nouvelles de la Science et des Technologies (1989), 7

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See detailVaricella-zoster virus infection of adult rat sensory neurons in vitro.
Merville-Louis, M. P.; Sadzot-Delvaux, Catherine ULg; Delree, P. et al

in Journal of Virology (1989), 63(7), 3155-60

We report here an in vitro model of neuronal infection by varicella-zoster virus (VZV). Such a model has been achieved by using dissociated adult rat dorsal root ganglia cells infected by cocultivation ... [more ▼]

We report here an in vitro model of neuronal infection by varicella-zoster virus (VZV). Such a model has been achieved by using dissociated adult rat dorsal root ganglia cells infected by cocultivation with VZV-infected MRC5 cells or with cell-free virus. Indirect VZV immunolabeling, in situ hybridization, and neuron-specific immunolabeling demonstrated that VZV infection occurred selectively in neurons. VZV-specific immunolabeling detected a few neurons 1 or 2 days postinfection but not later. Genome detection using cloned VZV DNA probes revealed a hybridization signal primarily with RNA. Within 1 to 6 days postinfection, a progressive increase of VZV-specific hybridization was observed in up to 50% of the neurons. RNAs corresponding to immediate-early, early, and late genes were found, and transcripts of immediate-early gene 63 were particularly abundant. [less ▲]

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See detailImmunomodulation of varicella-zoster virus antigens
Marc, Ph.; Sadzot-Delvaux, Catherine ULg; Merville-Louis, M. P. et al

Poster (1989)

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See detailIn vivo model of varicella-zoster virus latency in the nervous system
Sadzot-Delvaux, Catherine ULg; Merville-Louis, Marie Paule; Delrée, P. et al

Conference (1989)

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See detailAcute experimental glomerulonephritis induced by the glomerular deposition of circulating polymerid IgA-Concanavalin A complexes
Davin, J.-C.; Dechenne, Charles ULg; Lombet, Jacques ULg et al

in Virchows Archiv. A : Pathological Anatomy and Histopathology (1989), 415(1), 7-20

The perfusion of polymeric or secretory IgA-Concanavalin A complexes into the aorta of rats led to a mannose-dependent binding of both IgA and lectin to the glomerular capillary wall, as shown by double ... [more ▼]

The perfusion of polymeric or secretory IgA-Concanavalin A complexes into the aorta of rats led to a mannose-dependent binding of both IgA and lectin to the glomerular capillary wall, as shown by double immunolocalization experiments, by quantitative analysis of the amount of radiolabeled complexes bound per g of kidney, and by blocking experiments with the corresponding carbohydrate. Rats injected with amounts of those complexes as low as 500 ?g developed, one hour later, a focal and segmental proliferative glomerulonephritis characterized by the deposition of injected complexes and of rat C3 and rat fibrin/ fibrinogen in most glomeruli ; focal thrombosis and small areas of necrosis in 10 to 15% of glomeruli, confined to the periphery of a single lobule of the tuft and segmental infiltration of these glomeruli by polymorphonuclear leucocytes and platelets. At the same time, many mesangial cells exhibited a hyperactive appearance, and red blood cells were noted in tubular lumens. In contrast, rats similarly injected with either monomeric IgA-ConA complexes, multimeric or secretory IgA-peanut agglutinin complexes or polymeric or monomeric IgA aggregates of comparable apparent molecular weight did not develop obvious glomerular lesions within one hour. The data indicate that preformed polymeric IgA-ConA complexes can specifically bind to glomerular structures in vivo and trigger acute glomerular lesions locally, analogous to those observed in some glomerular diseases associated with a cryoglobulinemia. [less ▲]

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See detailLatent infection in vivo by the varicella-zoster virus (VZV) in the rat nervous system
Sadzot-Delvaux, Catherine ULg; Merville, Marie-Paule ULg; Delrée, P. et al

in Archives Internationales de Physiologie et de Biochimie (1989), 97

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See detailAntibodies to varicella-zoster virus modulate antigen distribution in cultured infected cells
Marc, Ph.; Sadzot-Delvaux, Catherine ULg; Merville, Marie-Paule ULg et al

in Archives Internationales de Physiologie et de Biochimie (1989), 97

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