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See detailTwo-dimensional study of varicella-zoster virus proteins
Debrus, S.; Duquesne, Patricia ULg; Sadzot-Delvaux, Catherine ULg et al

Poster (1992)

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See detailGranulomatous reactions following herpes-zoster contain varicella-zoster glycoprotein GPI
Nikkels, Arjen ULg; Sadzot-Delvaux, Catherine ULg; Cloes, Jean-Michel et al

in Journal of Investigative Dermatology (1992), 98(4), 522

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See detailCharacterization of regulatory functions of the varicella-zoster virus gene-63-encoded protein
Jackers, Pascale ULg; Defechereux, Patricia; Baudoux, Laurence et al

in Journal of Virology (1992), 66(6), 3899-3903

Varicella-zoster virus (VZV) gene 63 encodes a protein (IE63) with a predicted molecular mass of 30.5 kDa which has amino acid similarities to the immediate-early (IE) protein 22 (ICP22) of herpes simplex ... [more ▼]

Varicella-zoster virus (VZV) gene 63 encodes a protein (IE63) with a predicted molecular mass of 30.5 kDa which has amino acid similarities to the immediate-early (IE) protein 22 (ICP22) of herpes simplex virus type 1. ICP22 is a polypeptide synthesized in herpes simplex virus type 1-infected cells, and as is the case for its VZV counterpart, its regulatory functions are unknown. On the basis of the VZV DNA sequence, it has been shown that IE63 exhibits hydrophilic and acidic properties, suggesting that this protein could play a regulatory role during the infectious cycle. We report in this article cotransfection experiments which demonstrate that the VZV gene 63 protein strongly represses, in a dose-dependent manner, the expression of VZV gene 62. On the other hand, transient expression of the VZV gene 63 protein can promote activation of the thymidine kinase gene but cannot affect the expression of the genes encoding glycoproteins I and II. The results of transient expression experiments strongly suggest that the VZV gene 63 protein could play a pivotal role in the repression of IE gene expression as well as in the activation of early gene expression [less ▲]

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See detailVaricella-zoster gene 63 encoded protein is an important regulatory factor
Jackers, Pascale ULg; Defechereux, Patricia; Baudoux, Laurence et al

in Archives Internationales de Physiologie, de Biochimie et de Biophysique (1992), 100

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See detailStudy of a new presumed mitochondrial carrier, the product of the yeast RIM 2 gene
Hellin, E.; Van Dyck, E.; Duyckaerts, Claire ULg et al

in Archives Internationales de Physiologie, de Biochimie et de Biophysique (1992), 100

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See detailHIV-1 reactivation after an oxidative stress
Legrand, Sylvie ULg; Vaira, Dolorès ULg; Rentier, Bernard ULg et al

in LinkVIII International Conference on AIDS/III STD World Congress, Amsterdam, the Netherlands 19-24 July 1992 (1992)

OBJECTIVES: A common denominator shared by several HIV-1 reactivation agents such as certain cytokines, UV irradiation and heat shock is their ability to cause stress response. Consequently, we have ... [more ▼]

OBJECTIVES: A common denominator shared by several HIV-1 reactivation agents such as certain cytokines, UV irradiation and heat shock is their ability to cause stress response. Consequently, we have investigated the effects of oxidative stress on HIV-1 reactivation, knowing that HIV-1 latently infected T cells can be exposed in vivo to such a stress when blood phagocytes are stimulated during inflammatory reactions. METHODS: The promonocytic (U1) and lymphocytic (ACH-2) cell lines, both HIV-1 chronically infected, were used to study the reactivation phenomenon. To test wether HIV-1 reactivation is mediated by LTR transactivation, the HeLa HIV-1 CAT cell line, which carries an integrated DNA cartridge containing CAT gene under control of HIV-1 LTR, was also exposed to an oxidative stress. RESULTS: Hydrogen peroxide exposure of U1 cells leads to an increased reverse transcriptase (RT) activity in supernatant fluid. Over the optimal concentrations range (0.5 to 1 mM), a four to fivefold stimulation level is reached. Below these concentrations, stress conditions are not sufficient and above, they induce a too important lethal effect. Immunofluorescence carried out on stressed U1 cells shows that H2O2 leads to HIV-1 gene expression activation and not to a release of viral particles from damaged cells. H2O2 also induces a stimulation of CAT activity in HeLa HIV-1 CAT cells. Intracellular singulet oxygen (1O2) is also able to induce an increase of RT activity in supernatant fluid of U1 and ACH-2 cells and a stimulation of CAT activity in HeLa HIV-1 CAT cells. A dose-response curve can also be demonstrated. In order to transpose these in vitro experiments to situations encountered in vivo, activated phagocytes were cocultivated with HeLa HIV-1 CAT cells. A weak stimulation of CAT activity was detected. CONCLUSIONS: Cellular oxidative damages induce HIV-1 LTR transactivation leading to viral gene expression and consequently to a burst of virus production. DNA damages induced by oxidative stress could be at the onset of HIV-1 reactivation. Experiments are now in progress to elucidate the mechanisms leading to HIV-1 reactivation after an oxidative stress. [less ▲]

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See detailHIV-1 reactivation after an oxidative stress
Legrand, Sylvie ULg; Hoebeke, Maryse ULg; Vaira, Dolorès ULg et al

in Archives Internationales de Physiologie, de Biochimie et de Biophysique (1992), 100

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See detailVaricella-zoster virus: an ultrastructural study of the assembly phases
Schoonbroodt, Sonia; Piette, Jacques ULg; Rentier, Bernard ULg

in Archives Internationales de Physiologie, de Biochimie et de Biophysique (1992), 100

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See detailTwo-dimensional study of varicella-zoster virus proteins
Debrus, S.; Lebon, L.; Schoonbroodt, Sonia et al

in Archives Internationales de Physiologie, de Biochimie et de Biophysique (1992), 100(2), 39

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See detailMolecular characterization of varicella-zoster virus gene expression
Defechereux, Patricia; Baudoux, Laurence; Jackers, Pascale ULg et al

in Archives Internationales de Physiologie, de Biochimie et de Biophysique (1992), 100

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See detailVZV glycoproteins gpI and gpII are present in dermal cells without their corresponding genome
Nikkels, Arjen ULg; Delvenne, Philippe ULg; Debrus, S. et al

in Journal of Cutaneous Pathology (1992), 19

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See detailParasite communities: Patterns and Processes
Rentier, Bernard ULg

in Book Reviews, Biochemical Systematics and Ecology (G. Esch, A. Bush & J. Aho, eds., Chapman & Hall, 1990) (1991)

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See detailAn in vivo model of varicella-zoster virus latent infection of dorsal root ganglia
Sadzot-Delvaux, Catherine ULg; Merville, Marie-Paule ULg; Delrée, P. et al

in Journal of Neuroscience Research (1990), 26(1), 83-89

We describe here the first in vivo model of varicella-zoster virus (VZV) latent infection in the adult rat peripheral nervous system. Infected Mewo cells were injected subcutaneously along the spine of ... [more ▼]

We describe here the first in vivo model of varicella-zoster virus (VZV) latent infection in the adult rat peripheral nervous system. Infected Mewo cells were injected subcutaneously along the spine of healthy adult rats. No clinical sign of infection was observed even 9 months after inoculation. Humoral immune response to VZV was detected in all infected animals throughout the study (9 months). The presence of viral material in dissociated and cultured dorsal root ganglia (DRG) from inoculated animals was studied by immunoperoxidase and in situ hybridization. When DRGs from infected animals were plated in culture from 1 month and up to 9 months after inoculation, viral nucleic acids and proteins were detected in neurons. Furthermore, trypsinization and subcultivation of infected neurons in culture is needed to reactivate infectious virus at least in some of the neurons. This model provides a useful tool for studying 1) the molecular mechanisms leading to an in vivo latency, 2) the role of the immune system, in particular cellular immunity, on the establishment, maintenance, and reactivation of latency, 3) the neurotropism of mutant viruses, and 4) the effects of antiviral agents. [less ▲]

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See detailAcute and persistent varicella-zoster virus infection of human and murine neuroblastoma cell lines
Bourdon-Wouters, C.; Merville, Marie-Paule ULg; Sadzot-Delvaux, Catherine ULg et al

in Journal of Neuroscience Research (1990), 26(1), 90-97

Human and murine neuroblastoma cell lines were infected in vitro with varicella-zoster virus (VZV). Infected human neuroblastoma cells (IMR-32) supported the synthesis of abundant viral antigens as ... [more ▼]

Human and murine neuroblastoma cell lines were infected in vitro with varicella-zoster virus (VZV). Infected human neuroblastoma cells (IMR-32) supported the synthesis of abundant viral antigens as detected by indirect immunoperoxidase labeling using human serum rich in anti-VZV antibodies and did not survive the infection. In situ hybridization (ISH) with VZV-cloned probes revealed a strong hybridization signal in these infected cells. During cultivation, the virus was released in the culture medium, and viral polypeptides were revealed by Western blotting of infected cells, using either a monoclonal anti-gpI antibody or a rabbit antiserum. All these findings indicate that IMR-32 cells support a productive and lytic infection by VZV, whether infected by cell-free virus or by cocultivation with infected cells. Murine neuroblastoma cells (neuro-2A) survived VZV infection and did not produce any infectious virus. No VZV-specific proteins were detected in infected cells either by immunolabeling or by Western blotting. However, viral nucleic acids could be detected by ISH, indicating that mouse neuroblastoma cells displayed a nonproductive, nonlytic infection. Infected neuro-2A cells have been examined by ISH using probes corresponding to immediate early (IE) genes 4, 62, and 63 and late (L) gene 31 encoding gpII. A strong hybridization signal was detected when infected cells were probed with a fragment containing the IE genes 62 and 63. Lower levels of hybridization were detected with the other probes, corresponding to IE or L genes. These systems allow comparative molecular analysis of persistent and acute infection of nerve cells by VZV. [less ▲]

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See detailTyping of human papillomaviruses in genital specimens by DNA hybridization
Lauricella, M.-A.; Piette, Jacques ULg; Lifrange, Eric ULg et al

in Archives Internationales de Physiologie et de Biochimie (1990), 98(2), 34

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See detailReactivation of HIV-1 after an oxidative stress
Piette, Jacques ULg; Legrand-Poels, Sylvie; Vaira, Dolorès ULg et al

in Free Radicals in Biology and Medicine (1990), 9(Suppl. A), 5

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See detailDiagnosis by PCR of HIV-1 infection in seronegative individuals at risk
Vaira, Dolorès ULg; François-Gérard, Ch.; Doppagne, A. et al

in AIDS Research and Human Retroviruses (1990), 6(2), 173-174

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See detailImmunological Techniques in Insect Biology (L.I. Gilbert & T.A. Miler, eds.)
Rentier, Bernard ULg

in Biochemical Systematics & Ecology (1989), 17(6), 501-502

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See detailNon parallel sigmoids reflect a competition effect in solid phase immunoassay
François-Gérard, C.; Gérard, P.; Rentier, Bernard ULg

in Rivat, Claude; Stoltz, Jean-François (Eds.) Biotechnologie des protéines du plasma : purification et utilisations cliniques et biologiques, symposium international INSERM, Nancy, 17-19 mai 1988 (1989)

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