References of "Rentier, Bernard"
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See detailTyping of human papillomaviruses in genital specimens by DNA hybridization
Lauricella, M.-A.; Piette, Jacques ULg; Lifrange, Eric ULg et al

in Archives Internationales de Physiologie et de Biochimie (1990), 98(2), 34

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See detailReactivation of HIV-1 after an oxidative stress
Piette, Jacques ULg; Legrand-Poels, Sylvie; Vaira, Dolorès ULg et al

in Free Radicals in Biology and Medicine (1990), 9(Suppl. A), 5

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See detailDiagnosis by PCR of HIV-1 infection in seronegative individuals at risk
Vaira, Dolorès ULg; François-Gérard, Ch.; Doppagne, A. et al

in AIDS Research and Human Retroviruses (1990), 6(2), 173-174

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See detailImmunological Techniques in Insect Biology (L.I. Gilbert & T.A. Miler, eds.)
Rentier, Bernard ULg

in Biochemical Systematics & Ecology (1989), 17(6), 501-502

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See detailMéthodes de détection de l'infection par le virus de l'immunodéficience humaine (HIV)
François-Gérard, Ch.; Warling, Ch.; Vaira, Dolorès ULg et al

in Revue Médicale de Liège (1989), 44(8), 295-305

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See detailIntérêt du typage viral des papillomavirus humains (HPV) en relation avec les neoplasies des muqueuses genitales
Lifrange, Eric ULg; Lauricella, M.; Rentier, Bernard ULg et al

in Revue Médicale de Liège (1989), 44(1), 1-14

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See detailDiagnosis of HIV-1 in African couples : comparison of serology and PCR
Vaira, Dolorès ULg; Sondag, Danièle ULg; François-Gérard, C. et al

in Cinquième conférence internationale sur le SIDA : le défi scientifique et social (1989)

OBJECTIVE: Search for the rate of HIV-1 contamination among seronegative sexual partners of seropositive individuals. METHODS: Classical serological methods (EIA, WB) and PCR. SERIES: 36 heterosexual ... [more ▼]

OBJECTIVE: Search for the rate of HIV-1 contamination among seronegative sexual partners of seropositive individuals. METHODS: Classical serological methods (EIA, WB) and PCR. SERIES: 36 heterosexual couples from central Africa, accounting for a total of 73 persons: 13/37 seropositive women, 23/36 seropositive men. All couples were serologically discordant, i.e. one partner was seropositive. CONCLUSIONS: In such a population, particularly at risk of HIV contamination, the rate of false negative serological diagnosis reached 70%. [less ▲]

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See detailDiagnostic des infections génitales à Papillomavirus
Lifrange, Eric ULg; Rentier, Bernard ULg

Conference (1989)

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See detailAbsence of seroconversion in a PCR positive person 18 months after transfusion of HIV infected blood
Vaira, Dolorès ULg; François-Gérard, C.; Rentier, Bernard ULg et al

in Vox Sanguinis (1989), 57(3), 220-221

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See detailEvaluation d'une nouvelle méthode pour le dépistage précoce du cancer colo-rectal
Rentier, Bernard ULg; Collin-Marcq, O.

in Nouvelles de la Science et des Technologies (1989), 7

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See detailVaricella-zoster virus infection of adult rat sensory neurons in vitro.
Merville-Louis, M. P.; Sadzot-Delvaux, Catherine ULg; Delree, P. et al

in Journal of Virology (1989), 63(7), 3155-60

We report here an in vitro model of neuronal infection by varicella-zoster virus (VZV). Such a model has been achieved by using dissociated adult rat dorsal root ganglia cells infected by cocultivation ... [more ▼]

We report here an in vitro model of neuronal infection by varicella-zoster virus (VZV). Such a model has been achieved by using dissociated adult rat dorsal root ganglia cells infected by cocultivation with VZV-infected MRC5 cells or with cell-free virus. Indirect VZV immunolabeling, in situ hybridization, and neuron-specific immunolabeling demonstrated that VZV infection occurred selectively in neurons. VZV-specific immunolabeling detected a few neurons 1 or 2 days postinfection but not later. Genome detection using cloned VZV DNA probes revealed a hybridization signal primarily with RNA. Within 1 to 6 days postinfection, a progressive increase of VZV-specific hybridization was observed in up to 50% of the neurons. RNAs corresponding to immediate-early, early, and late genes were found, and transcripts of immediate-early gene 63 were particularly abundant. [less ▲]

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See detailImmunomodulation of varicella-zoster virus antigens
Marc, Ph.; Sadzot-Delvaux, Catherine ULg; Merville-Louis, M. P. et al

Poster (1989)

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See detailIn vivo model of varicella-zoster virus latency in the nervous system
Sadzot-Delvaux, Catherine ULg; Merville-Louis, Marie Paule; Delrée, P. et al

Conference (1989)

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See detailAcute experimental glomerulonephritis induced by the glomerular deposition of circulating polymerid IgA-Concanavalin A complexes
Davin, J.-C.; Dechenne, Charles ULg; Lombet, Jacques ULg et al

in Virchows Archiv. A : Pathological Anatomy and Histopathology (1989), 415(1), 7-20

The perfusion of polymeric or secretory IgA-Concanavalin A complexes into the aorta of rats led to a mannose-dependent binding of both IgA and lectin to the glomerular capillary wall, as shown by double ... [more ▼]

The perfusion of polymeric or secretory IgA-Concanavalin A complexes into the aorta of rats led to a mannose-dependent binding of both IgA and lectin to the glomerular capillary wall, as shown by double immunolocalization experiments, by quantitative analysis of the amount of radiolabeled complexes bound per g of kidney, and by blocking experiments with the corresponding carbohydrate. Rats injected with amounts of those complexes as low as 500 ?g developed, one hour later, a focal and segmental proliferative glomerulonephritis characterized by the deposition of injected complexes and of rat C3 and rat fibrin/ fibrinogen in most glomeruli ; focal thrombosis and small areas of necrosis in 10 to 15% of glomeruli, confined to the periphery of a single lobule of the tuft and segmental infiltration of these glomeruli by polymorphonuclear leucocytes and platelets. At the same time, many mesangial cells exhibited a hyperactive appearance, and red blood cells were noted in tubular lumens. In contrast, rats similarly injected with either monomeric IgA-ConA complexes, multimeric or secretory IgA-peanut agglutinin complexes or polymeric or monomeric IgA aggregates of comparable apparent molecular weight did not develop obvious glomerular lesions within one hour. The data indicate that preformed polymeric IgA-ConA complexes can specifically bind to glomerular structures in vivo and trigger acute glomerular lesions locally, analogous to those observed in some glomerular diseases associated with a cryoglobulinemia. [less ▲]

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See detailLatent infection in vivo by the varicella-zoster virus (VZV) in the rat nervous system
Sadzot-Delvaux, Catherine ULg; Merville, Marie-Paule ULg; Delrée, P. et al

in Archives Internationales de Physiologie et de Biochimie (1989), 97

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See detailAntibodies to varicella-zoster virus modulate antigen distribution in cultured infected cells
Marc, Ph.; Sadzot-Delvaux, Catherine ULg; Merville, Marie-Paule ULg et al

in Archives Internationales de Physiologie et de Biochimie (1989), 97

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See detailVaricella-Zoster virus infection in the nervous system: in vitro and in vivo models
Sadzot-Delvaux, Catherine ULg; Merville, Marie-Paule; Bourdon-Wouters, Christine et al

in Acta Neurologica Belgica (1989), 88

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See detailPreparation of reproducible alkaline phosphatase-antibody conjugates for enzyme immunoassay using a heterobifunctional linking agent
Jeanson, Antoinette; Cloes, Jean-Michel; Bouchet, Mireille et al

in Analytical Biochemistry (1988), 172(2), 392-396

Conjugates of alkaline phosphatase (AP) and mouse monoclonal immunoglobulins G (IgG) were prepared by means of the heterobifunctional linker, N-succinimidyl 3-(2-pyridyldithio)-propionate. The efficiency ... [more ▼]

Conjugates of alkaline phosphatase (AP) and mouse monoclonal immunoglobulins G (IgG) were prepared by means of the heterobifunctional linker, N-succinimidyl 3-(2-pyridyldithio)-propionate. The efficiency of such conjugates can be improved by optimizing the degree of substitution of IgG and AP. We have determined conditions yielding better performing conjugates than those synthesized by methods described previously. Moreover, the results obtained with the technique presented here are quite reproducible with all four monoclonal antibodies tested. [less ▲]

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See detailComparison of conjugation procedures for the preparation of monoclonal antibody-enzyme conjugates
Jeanson, Antoinette; Cloes, Jean-Michel; Bouchet, Mireille et al

in Journal of Immunological Methods (1988), 111(2), 261-270

Four monoclonal antibodies belonging to different subclasses and with differing isoelectric points were coupled to horseradish peroxidase (HRP) and alkaline phosphatase (AP) using various conjugation ... [more ▼]

Four monoclonal antibodies belonging to different subclasses and with differing isoelectric points were coupled to horseradish peroxidase (HRP) and alkaline phosphatase (AP) using various conjugation procedures. The conjugates were tested by enzyme immunoassay and their efficiency was characterized by the antibody and enzyme concentrations needed to obtain an arbitrary OD value. The suitability of antibody for conjugation through NH2 groups was tested by fluorodinitrobenzene (FDNB). HRP conjugates were produced by two variants of the sodium periodate procedure and two variants of the glutaraldehyde method, as well as by the heterobifunctional linker N-succinimidyl 3-(2-pyridyldithio)pro-pionate (SPDP). Two of the four antibodies were coupled by a third variant of the periodate method, through their carbohydrate moieties. The periodate-mediated conjugations, using sugar moieties on the enzyme, provided the most efficient HRP conjugates, regardless of the antibody subclass or isoelectric point. The glutaraldehyde procedures consistently gave the worst results. AP conjugates were prepared using the same methods. The most efficient and reproducible AP conjugates with all four monoclonal antibodies were obtained using the SPDP procedure. The efficiency of the other methods differed from one antibody to another. [less ▲]

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