Preparation of reproducible alkaline phosphatase-antibody conjugates for enzyme immunoassay using a heterobifunctional linking agent

in Analytical Biochemistry (1988), 172(2), 392-396

Conjugates of alkaline phosphatase (AP) and mouse monoclonal immunoglobulins G (IgG) were prepared by means of the heterobifunctional linker, N-succinimidyl 3-(2-pyridyldithio)-propionate. The efficiency ... [more ▼]

Conjugates of alkaline phosphatase (AP) and mouse monoclonal immunoglobulins G (IgG) were prepared by means of the heterobifunctional linker, N-succinimidyl 3-(2-pyridyldithio)-propionate. The efficiency of such conjugates can be improved by optimizing the degree of substitution of IgG and AP. We have determined conditions yielding better performing conjugates than those synthesized by methods described previously. Moreover, the results obtained with the technique presented here are quite reproducible with all four monoclonal antibodies tested. [less ▲]

Elucidation of non-parallel EIA curves

in Journal of Immunological Methods (1988), 111(1), 59-65

Quantitative determinations by EIA can be only obtained by reverse regression when linear portions of sample and standard curves are parallel. However, analysis of complex biological fluids often yields ... [more ▼]

Quantitative determinations by EIA can be only obtained by reverse regression when linear portions of sample and standard curves are parallel. However, analysis of complex biological fluids often yields sigmoid curves displaying lower slopes, thus invalidating any quantitative interpretation. We hypothesized that this phenomenon was due to a competition effect between the target (for example an antigen) and related molecules for the binding sites (for example a capture antibody) immobilized onto the solid phase. This has been confirmed experimentally using various target-to-competitor ratios and formulated as a mathematical model. The slope decrease in target detection was related to the proportion of competitor, not in a linear, but in an exponential manner. This mathematical model has been computerized and can be used to correct aberrant sample curves provided the relevant parameters have been previously determined in the same systems. A competition effect should be suspected whenever non-parallel EIA sigmoid slopes are obtained. [less ▲]

Persistent varicella-zoster virus infection in the nervous system: in vitro and in vivo models

in Acta Neurologica Belgica (1988), 88

In vitro and in vivo models of varicella-zoster virus persistence in the nervous system

Poster (1988)

Le système immunitaire : principes généraux

in Journal de Pharmacie de Belgique (1988), 43(3), 185-211

Production of heterobispecific monoclonal antibodies by mouse hybrid hybridomas (quadromas)

in Archives Internationales de Physiologie, de Biochimie et de Biophysique (1988), 96

Detection of varicella-zoster virus (VZV) genome in infected cells using cloned DNA probes

in Acta Neurologica Belgica (1987), 87

Molecular cloning of varicella-zoster virus DNA and its detection in situ in infected nerve cells

in Archives Internationales de Physiologie et de Biochimie (1987), 95(2), 31

Problems in quantitation of anti-viral IgG isotypes: a computerized mathematical model

in Charbonneau, K. (Ed.) Abstracts : sixth international congress of immunology, July 6-11,1986, Toronto, Canada (1986)

Investigations of the molecular mechanisms involved in measles virus persistence in differentiated nerve cell

Conference (1985)