References of "Remacle, Claire"
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See detailWood Acid Hydrolysate as a Feedstock for Chlorella Growth
Miazek, Krystian ULg; Goffin, Dorothée ULg; Richel, Aurore ULg et al

Scientific conference (2015, June 24)

In this work, the effect of beech (Fagus sylvatica) wood acid hydrolysate on growth of Chlorella sorokin-iana was evaluated. Experiments carried out in this study show that neutralized wood acid ... [more ▼]

In this work, the effect of beech (Fagus sylvatica) wood acid hydrolysate on growth of Chlorella sorokin-iana was evaluated. Experiments carried out in this study show that neutralized wood acid hydrolysate can vastly improve Chlorella growth, due to the presence of organic carbon. However, simultaneously the suppression of Chlorella growth at the onset of cultivation was observed, presumably due to inhibitory substances, and this effect was more pronounced with the increase of hydrolysate dosage. Beech wood acid hydrolysate can be a valuable feedstock to stimulate Chlorella growth, on condition that inhibitory level of hydrolysate loading is avoided. [less ▲]

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See detailMetabolomic analysis of the green microalga Chlamydomonasreinhardtii cultivated under day/night conditions
Willamme, Rémi ULg; Alsafra, Zouheir; Arumugam, Rameshkumar et al

in Journal of Biotechnology (2015)

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See detailImproving the sunlight-to-biomass conversion efficiency in microalgal biofactories
Wobbe, Lutz; Remacle, Claire ULg

in Journal of Biotechnology (2015), 201

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See detailIsolation of Chlamydomonas reinhardtii mutants with altered mitochondrial respiration by chlorophyll fluorescence measurement.
Massoz, Simon; Larosa, Véronique ULg; Horrion, Bastien et al

in Journal of biotechnology (2015)

The unicellular green alga Chlamydomonas reinhardtii is a model organism for studying energetic metabolism. Most mitochondrial respiratory-deficient mutants characterized to date have been isolated on the ... [more ▼]

The unicellular green alga Chlamydomonas reinhardtii is a model organism for studying energetic metabolism. Most mitochondrial respiratory-deficient mutants characterized to date have been isolated on the basis of their reduced ability to grow in heterotrophic conditions. Mitochondrial deficiencies are usually partly compensated by adjustment of photosynthetic activity and more particularly by transition to state 2. In this work, we explored the opportunity to select mutants impaired in respiration and/or altered in dark metabolism by measuring maximum photosynthetic efficiency by chlorophyll fluorescence analyses (FV/FM). Out of about 2900 hygromycin-resistant insertional mutants generated from wild type or from a mutant strain deficient in state transitions (stt7 strain), 22 were found to grow slowly in heterotrophic conditions and 8 of them also showed a lower FV/FM value. Several disrupted coding sequences were identified, including genes coding for three different subunits of respiratory-chain complex I (NUO9, NUOA9, NUOP4) or for isocitrate lyase (ICL1). Overall, the comparison of respiratory mutants obtained in wild-type or stt7 genetic backgrounds indicated that the FV/FM value can be used to isolate mutants severely impaired in dark metabolism. [less ▲]

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See detailChlorophyll: natural sources, extraction methods and application for textile industry
Miazek, Krystian ULg; Remacle, Claire ULg; Richel, Aurore ULg et al

Conference (2014, October 14)

Chlorophyll is a photosynthetic pigment available abundantly in microalgae and terrestrial plants. This pigment found applications in pharmaceutical, cosmetic and food products as a wound healing ... [more ▼]

Chlorophyll is a photosynthetic pigment available abundantly in microalgae and terrestrial plants. This pigment found applications in pharmaceutical, cosmetic and food products as a wound healing, antioxidant or coloring agent. Recent reports suggest that chlorophyll can also be used as a biomordant to enhance the dyeing process of textile products, but also as a textile dye with antimicrobial properties. In this presentation, different aspects of chlorophyll production are discussed. Firstly, numerous plant biomass types as potential sources of chlorophyll are presented. Subsequently, different methods for chlorophyll extraction and separation from plant biomass are described. Finally, possibilities of chlorophyll implementation into textile products on industrial scale are evaluated. [less ▲]

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See detailTranscriptomics and Proteomics of a Secondary Green Alga.
Perez, Emilie ULg; Lapaille, Marie; Degand, Hervé et al

Poster (2014, August)

Euglena gracilis is a secondary green alga related to trypanosomes that derives from a secondary endosymbiosis between a phagotrophic ancestor and a prasinophycean green alga. Our general objective is to ... [more ▼]

Euglena gracilis is a secondary green alga related to trypanosomes that derives from a secondary endosymbiosis between a phagotrophic ancestor and a prasinophycean green alga. Our general objective is to study the metabolic interactions established between the secondary plastid and the mitochondrion after the endosymbiotic event and to determine the phylogenetic origin of the genes encoding the proteins involved in the energetic pathways. As a first step, we analysed the subunit composition of the mitochondrial respiratory chain, both in silico and by targeted proteomics, to assess the extent of its similitude with the respiratory chain of Trypanosomatidae. We have shown that Euglena shares many additional subunits with trypanosomes, which suggests that these subunits are not especially associated to a parasitic lifestyle. As a second step, we sequenced the total transcriptome of Euglena and determined the phylogenetic origin of each predicted transcript using a database of about 1000 complete proteomes representing the diversity of life. These analyses confirmed that Euglena recruited its genes from a very diverse set of sources. As a third step, we performed a high-throughput analysis of the mitochondrial proteome of Euglena. Our MS/MS experiments, taking advantage of the availability of our transcriptome, mostly recovered mitochondrial proteins, which indicates that our mitochondrial extracts were quite pure. The identified proteins encompassed about 15 different mitochondrial pathways. We are now in the process of comparing the expression levels of both the transcripts and the corresponding proteins across a range of culture conditions selected to differently stimulate the mitochondrion and the plastid. [less ▲]

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See detailEffect of lignocellulose related compounds on microalgae growth and product biosynthesis: a review
Miazek, Krystian ULg; Remacle, Claire ULg; Richel, Aurore ULg et al

in Energies (2014), 7(2014), 4446-4481

Microalgae contain valuable compounds that can be harnessed for industrial applications. Lignocellulose biomass is a plant material containing in abundance organic substances such as carbohydrates ... [more ▼]

Microalgae contain valuable compounds that can be harnessed for industrial applications. Lignocellulose biomass is a plant material containing in abundance organic substances such as carbohydrates, phenolics, organic acids and other secondary compounds. As growth of microalgae on organic substances was confirmed during heterotrophic and mixotrophic cultivation, lignocellulose derived compounds can become a feedstock to cultivate microalgae and produce target compounds. In this review, different treatment methods to hydrolyse lignocellulose into organic substrates are presented first. Secondly, the effect of lignocellulosic hydrolysates, organic substances typically present in lignocellulosic hydrolysates, as well as minor co-products, on growth and accumulation of target compounds in microalgae cultures is described. Finally, the possibilities of using lignocellulose hydrolysates as a common feedstock for microalgae cultures are evaluated. [less ▲]

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See detailGrowth of Chlorella in the presence of organic carbon: A photobioreactor study
Miazek, Krystian ULg; Goffin, Dorothée ULg; Richel, Aurore ULg et al

Scientific conference (2014, June 19)

In this study, the effect of organic carbon supplementation at low light intensity on Chlorella sorokiniana growth was evaluated. Addition of 1 g/L of acetate to media gave the highest growth rate and ... [more ▼]

In this study, the effect of organic carbon supplementation at low light intensity on Chlorella sorokiniana growth was evaluated. Addition of 1 g/L of acetate to media gave the highest growth rate and provided stable high biomass culture during prolonged cultivation time. Glucose at 1 – 5 g/L also improved biomass growth rate, although stability of high biomass culture could not be achieved. Overall, the presence of organic carbon can considerably enhance Chlorella growth when low light intensity is applied. [less ▲]

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See detailA forward genetic screen to identify hydrogenase-deficient mutants in the unicellular green alga Chlamydomonas reinhardtii
Emonds-Alt, Barbara ULg; Godaux, Damien ULg; Cardol, Pierre ULg et al

Poster (2014, June 15)

The ability of the unicellular green alga Chlamydomonas reinhardtii to evolve molecular hydrogen (H2) is due to the presence of oxygen-sensitive Fe-hydrogenases (HydA1/2), expressed in anoxic conditions ... [more ▼]

The ability of the unicellular green alga Chlamydomonas reinhardtii to evolve molecular hydrogen (H2) is due to the presence of oxygen-sensitive Fe-hydrogenases (HydA1/2), expressed in anoxic conditions that drive the photosynthetic electron flow to reduce protons into H2. In order to identify new players involved in H2 photoproduction in Chlamydomonas, an insertion mutant library was generated using cassettes conferring resistance to hygromycin or paromomycin. Hydrogenase activity is physiologically relevant during a transition from dark anoxia to light. In dark anoxic conditions, the cellular redox poise is high and the photosynthetic electron transport chain is fully reduced. Upon illumination, hydrogenase activity allows the reoxidation of photosynthetic intersystem electron carriers until oxic conditions and carbon fixation ability are restored. We thus designed an in vivo fluorescence imaging screen based on the different kinetics of photosynthesis induction between wild type and hydrogenase-deficient mutants [1]. At this stage, three putative hydrogenase mutants have been identified on 10,000 transformants. Molecular characterization of the insertion site of the resistance cassette by TAIL-PCR and genetic analyses of the linkage between the antibiotic resistance and the fluorescence phenotype showed that one mutant was untagged with the resistance while two tagged mutants were deficient for the HydG assembly factor. [less ▲]

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See detailApplications des microalgues
Franck, Fabrice ULg; Remacle, Claire ULg

Conference (2014, May 22)

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See detailMitochondrial Proteomics of a Secondary Green Alga.
Perez, Emilie ULg; Degand, Hervé; Morsomme, Pierre et al

Poster (2014, May 06)

Euglena gracilis is an alga that derives from a secondary endosymbiosis with a green alga. Our general objective is to study the interactions established between the chloroplast and the mitochondrion ... [more ▼]

Euglena gracilis is an alga that derives from a secondary endosymbiosis with a green alga. Our general objective is to study the interactions established between the chloroplast and the mitochondrion during the endosymbiosic event and to determine the phylogenetic origin of the genes encoding the proteins involved in these interactions. As a first step, we performed a high-throughput analysis of the mitochondrial proteome of Euglena gracilis. Our MS/MS experiments mostly recover mitochondrial proteins representing 15 mitochondrial pathways, which indicates that our mitochondrial extracts are relatively pure, but the phylogenetic origins of the corresponding genes are surprisingly diverse. [less ▲]

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See detailNouvelle méthode de criblage de mutants respiratoires du complexe I chez Chlamydomonas
Massoz, Simon ULg; Larosa, véronique; bailleul, benjamin et al

Scientific conference (2014, May 06)

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See detailLack of isocitrate lyase in Chlamydomonas leads to changes in carbon metabolism and in the response to oxidative stress under mixotrophic growth.
Plancke, Charlotte; Vigeolas, Hélène ULg; Hohner, Ricarda et al

in The Plant journal : for cell and molecular biology (2014), 77(3), 404-417

Isocitrate lyase is a key enzyme of the glyoxylate cycle. This cycle plays an essential role in cell growth on acetate, and is important for gluconeogenesis as it bypasses the two oxidative steps of the ... [more ▼]

Isocitrate lyase is a key enzyme of the glyoxylate cycle. This cycle plays an essential role in cell growth on acetate, and is important for gluconeogenesis as it bypasses the two oxidative steps of the tricarboxylic acid (TCA) cycle in which CO2 is evolved. In this paper, a null icl mutant of the green microalga Chlamydomonas reinhardtii is described. Our data show that isocitrate lyase is required for growth in darkness on acetate (heterotrophic conditions), as well as for efficient growth in the light when acetate is supplied (mixotrophic conditions). Under these latter conditions, reduced acetate assimilation and concomitant reduced respiration occur, and biomass composition analysis reveals an increase in total fatty acid content, including neutral lipids and free fatty acids. Quantitative proteomic analysis by 14 N/15 N labelling was performed, and more than 1600 proteins were identified. These analyses reveal a strong decrease in the amounts of enzymes of the glyoxylate cycle and gluconeogenesis in parallel with a shift of the TCA cycle towards amino acid synthesis, accompanied by an increase in free amino acids. The decrease of the glyoxylate cycle and gluconeogenesis, as well as the decrease in enzymes involved in beta-oxidation of fatty acids in the icl mutant are probably major factors that contribute to remodelling of lipids in the icl mutant. These modifications are probably responsible for the elevation of the response to oxidative stress, with significantly augmented levels and activities of superoxide dismutase and ascorbate peroxidase, and increased resistance to paraquat. [less ▲]

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