References of "Ramery, Eve"
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See detailPTX3: a new marker for local inflammation ?
Ramery, Eve ULiege; Bureau, Fabrice ULiege; Art, Tatiana ULiege et al

in Proceedings of the 11th ECVCP congress, Thessaloniki, Grece (2009)

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See detailDust exposure exacerbates the expression of ptx3, a recently discovered acute phase protein, in horses’ airways
Ramery, Eve ULiege; Bureau, Fabrice ULiege; Lekeux, Pierre ULiege

Conference (2009)

Heaves is a common cause of recurrent airway obstruction (R.A.O) that occurs in mature horses stabled in a dusty environment. The long pentraxin PTX3 play an important role in the host defence and over ... [more ▼]

Heaves is a common cause of recurrent airway obstruction (R.A.O) that occurs in mature horses stabled in a dusty environment. The long pentraxin PTX3 play an important role in the host defence and over-expression of PTX3 may contribute to airways injury. Therefore, we hypothesised that PTX3 may have relevance in the understanding of the pathogenesis of recurrent airway obstruction (R.A.O). In the present study, we first investigated the equine PTX3 (ePTX3) structure by cloning, sequencing and western-blotting. A 120pb deletion was found in the second exon of ePTX3 in comparison to human PTX3. Moreover, an alternative splicing occurs with complete deletion of the second exon, resulting in two forms of the protein, “spliced” (32 kD) and “full length” (42 kD). The pentraxin domain is very well conserved in the two forms with 94% of amino-acids conserved between equines and humans. These findings could have implications for the comprehension of the function of each domain of the protein. Then, we studied the effects of dust exposure and subsequent R.A.O crisis on ePTX3 expression in the broncho-alveolar lavage fluid (BALF) and the bronchial epithelial cells. PTX3 could be detected in BALF macrophages, neutrophils, eosinophils and bronchial epithelial cells. It was over-expressed in the BALF supernatant from RAO-affected horses in crisis. Dust exposure induced PTX3 in BALF macrophages either in healthy horses or in R.A.O-affected horses. Conversely, PTX3 was strongly over-expressed in the bronchial epithelial cells from R.A.O-affected horses in crisis. Considering epithelial cells as one of the major cell types in the airways, PTX3 produced at this level may play an important role in inflammatory process. Moreover, it suggests that dust-induced expression of PTX3 is differentially regulated in macrophages and bronchial-epithelial cells. A JNK-dependant, NFκB-independent regulatory pathway has been described in lung epithelial cells. A comparable mechanism could be involved in bronchial epithelial cells. If this is confirmed, PTX3 expression in respiratory epithelial cells could be therapeutically targeted. [less ▲]

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See detailExpression génique et médecine vétérinaire
Ramery, Eve ULiege; Van Erck, Emmanuelle ULiege; Bureau, Fabrice ULiege et al

in Proceedings des 36èmes Journées AVEF (2008)

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See detailHow transcriptomic studies may help to improve the control of bovine diseases : an example with calf pneumonia and endotoxemia
Wallemacq, Hugues ULiege; Ramery, Eve ULiege; Bureau, Fabrice ULiege et al

in Proceedings: XXVth Jubilee World Buiatrics Congress (2008)

Global change in genes expression induced by pathological processes can now be analysed in cattle by new tools called microarrays. These transcriptomic studies may help to better understand the ... [more ▼]

Global change in genes expression induced by pathological processes can now be analysed in cattle by new tools called microarrays. These transcriptomic studies may help to better understand the pathophysiological mechanisms responsible for the disease and may therefore contribute to develop more efficient preventive and curative strategies. An example is given with a model of calf pneumonia and endotoxemia [less ▲]

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See detailUsing a human microarray to highlight new genes of interest for a better understanding of molecular mechanisms that underpin the physiopathology of heaves
Ramery, Eve ULiege; Closset, Rodrigue; Bureau, Fabrice ULiege et al

in Proceedings: Plant and Animal Genome Conference, Equine Workshop, San Diego (2008)

Environmental causes of heaves are well described, but the molecular mechanisms of the disease remain unclear. Using reverse transcription polymerase chain reaction (RT-PCR), disparate results have been ... [more ▼]

Environmental causes of heaves are well described, but the molecular mechanisms of the disease remain unclear. Using reverse transcription polymerase chain reaction (RT-PCR), disparate results have been obtained concerning cytokines expression profile. cDNA microarray appears to be so far the platform of choice for massively parallel gene expression profiling and provides a good tool for exploratory research. However, equine-specific microarrays are not yet available on the market. Because they are commercially available, highly specific and well annotated, human and mouse large-scale microarrays are an exploratory alternative to equine-specific microarrays. In the present study, the purpose was to highlight new targets not previously related to the disease and able to improve our understanding of the molecular mechanisms of the disease. A human microarray was used to study gene expression in nucleated cells originating from peripheral blood and bronchoalveolar lavage fluid in heaves-affected horses. With a four-fold cut-off, a total of 46 candidates were identified with differentially regulated genes between heaves-affected horses and controls. Based on their documented function, five of these genes were selected for the real-time quantitative RT-PCR (RT-qPCR) validation procedure: CYBB, BTG1, MARCKS, PTX3 and PTPRC. The RT-qPCR results confirmed those obtained with the microarray, pointing out these genes as new directions for future experiments. However, the human microarray failed to detect the presence of IL-1beta and Il-8, otherwise confirmed by RT-qPCR, and the expression profile of the disease could not be obtained [less ▲]

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See detailEvaluation de la condition physique chez le cheval d’endurance : comparaison d’un test de terrain adapté et d’un test sur tapis roulant
Fraipont, Audrey ULiege; Van Erck, Emmanuelle; Toussaint, Marie et al

in 36èmes Journées AVEF (2008)

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See detailEtude de l'administration par inhalation de cefquinome chez le cheval sain
Art, Tatiana ULiege; Van Erk, E.; Ramery, Eve ULiege et al

in Pratique Vétérinaire Equine (2008), 40

Cefquinome aerosol is sometimes used in horse suffering from respiratory infections. However, harmlessness and validity of the method has never been studied. This work aimed at controlling the lack of ... [more ▼]

Cefquinome aerosol is sometimes used in horse suffering from respiratory infections. However, harmlessness and validity of the method has never been studied. This work aimed at controlling the lack of pulmonary effects of cefquinome when administered through inhalation in healthy horses and at comparing the cefquinome concentrations obtained in broncho-alveolar lavage after aerosol, intramuscular and intravenous administrations. A single aerosol of cefquinome did not induce any detectable side effect in healthy horses and allowed to obtain broncho-alveolar concentrations higher than obtained with intravenous and intramuscular injection. These results should now be completed by further studies, especially on horses suffering from respiratory disease and bronchial hyperreactivity [less ▲]

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See detailRelevance of using a human microarray to study gene expression in heaves-affected horses.
Ramery, Eve ULiege; Closset, Rodrigue; Bureau, Fabrice ULiege et al

in Veterinary Journal (2008), 177(2), 216-221

Environmental causes of heaves are well described, but the molecular mechanisms of the disease remain unclear. Previous studies have highlighted the implications of variations in gene expression, most ... [more ▼]

Environmental causes of heaves are well described, but the molecular mechanisms of the disease remain unclear. Previous studies have highlighted the implications of variations in gene expression, most using reverse transcription polymerase chain reaction (RT-PCR). This well-known technique limits the number of genes that can be studied in a single assay. Microarray appears to be a valuable tool to by-pass this limitation, but so far there has been no equine-specific microarray available on the market. The present study was performed to determine whether a human microarray could be used to study gene expression in nucleated cells originating from peripheral blood and bronchoalveolar lavage fluid (BALF) in heaves-affected horses. With a four-fold cut-off, a total of 46 candidates were identified with differentially regulated genes between heaves-affected horses and controls. A real-time quantitative RT-PCR (RT-QPCR) conducted on a selection of genes, determined on the basis of previous publications, was used to validate the microarray results. The microarray failed to detect the presence of interleukin (IL)-1beta and IL-8 mRNA in the nucleated cells from BALF otherwise confirmed by real-time RT-QPCR. Although some candidate genes have been identified using this method, a complete expression profile of genes related to heaves could not be obtained with the use of the human microarray. [less ▲]

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See detailUse of a human microarray to highlight new genes of interest for a better understanding of recurrent airway obstruction in horses (heaves)
Ramery, Eve ULiege; Closset, R.; Bureau, Fabrice ULiege et al

in XVIth International Plant & Animal Genome Conference (2008)

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See detailReport of the 3rd Havemeyer workshop on allergic diseases of the Horse, Holar, Iceland, June 2007
Marti, E.; Gerber, V.; Wilson, A. D. et al

in Veterinary Immunology and Immunopathology (2008), 126(3-4), 351-361

Allergic diseases occur in most mammals, although some species such as humans, dogs and horses seem to be more prone to develop allergies than others. In horses, insect bite hypersensitivity (IBH), an ... [more ▼]

Allergic diseases occur in most mammals, although some species such as humans, dogs and horses seem to be more prone to develop allergies than others. In horses, insect bite hypersensitivity (IBH), an allergic dermatitis caused by bites of midges, and recurrent airway obstruction (RAO), a hyperreactivity to stable born dust and allergens, are the two most prevalent allergic diseases. Allergic diseases involve the interaction of three major factors: (i) genetic constitution, (ii) exposure to allergens, and (iii) a dysregulation of the immune response determined by (i) and (ii). However, other environmental factors such as infectious diseases, contact with endotoxin and degree of infestation with endoparasites have been shown to influence the prevalence of allergic diseases in humans. How these factors may impact upon allergic disease in the horse is unknown at this time. The 3rd workshop on Allergic Diseases of the Horse, with major sponsorship from the Havemeyer Foundation, was held in Holar, Iceland, in June 2007 and focussed on immunological and genetic aspects of IBH and RAO. This particular venue was chosen because of the prevalence of IBH in exported Icelandic horses. The incidence of IBH is significantly different between Icelandic horses born in Europe or North America and those born in Iceland and exported as adults. Although the genetic factors and allergens are the same, exported adult horses show a greater incidence of IBH. This suggests that environmental or epigenetic factors may contribute to this response. This report summarizes the present state of knowledge and summarizes important issues discussed at the workshop. [less ▲]

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See detailBilan medico-sportif du yearling a l’entrainement
Toussaint; Fraipont, Audrey ULiege; Wagemans, Marie-Christine et al

in 35èmes Journées AVEF (2007)

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See detailPotential use of micro-array technology (bio-puce) in the diagnosis of inflammatory disorders in the horse
Lekeux, Pierre ULiege; Thomas, A.; Ramery, Eve ULiege et al

in XVII. Tagung über Pferdekrankheiten im Rahmen der EQUITANA (2007)

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See detailIntérêt des tests sportifs dans le dépistage des affections respiratoires sub-cliniques chez le trotteur français à l’entraînement
Richard, E.; Fortier, G.; Fraipont, Audrey ULiege et al

in 35èmes Journées AVEF (2007)

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See detailAdministration par inhalation de cefquinome chez le cheval sain : effets sur la fonction respiratoire et concentrations dans le liquide pulmonaire
Wagemans, M.; Lekeux, Pierre ULiege; Van Erck, E. et al

in 35èmes Journées AVEF (2007)

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See detailEvaluation de l’efficacité du Caprofène lors de bronchoneumonie expérimentale bovine à Mannheimia haemolytica
Wallemacq, Hugues; Boutet, Philippe; Zecchinon, L. et al

in Revue de Médecine Vétérinaire (2007), 158(8-9), 418-424

M. haemolytica serotype A1 (formerly known as Pasteurella) is the most important and commonly isolated bacterial pathogen from fatal cases of bovine fibrinous pleuroneumonia. M. haemolytica and its two ... [more ▼]

M. haemolytica serotype A1 (formerly known as Pasteurella) is the most important and commonly isolated bacterial pathogen from fatal cases of bovine fibrinous pleuroneumonia. M. haemolytica and its two principal toxins, the lipopolysaccharides (LPS) and the leukotoxin (LktA) induce the recruitment, the activation and the necrosis of neutrophils involved in the pathogenicity of bovine pneumonic mannheimiosis (BPM). The objective of this study was to determine whether systemic therapy with carprofène (Rimadyl®*), a non-steroidal anti-inflammatory drug, improves the disease development in an acute experimental model of BPM. The experimental pathology was induced by trans-tracheal inoculation of M. haemolytica and toxins at T0. One hour post-inoculation, six calves were treated intravenously with carprofen (1,4 mg/kg) while six placebo-treated calves received dose-matched volumes of sterile saline. The clinical and biochemical parameters were measured at one (T+1), three (T+3) and seven (T+7) hours after inoculation. Disease scores for carprofen treated calves were significantly lower than those for placebo-treated controls six hours (T+7) after treatment. These results were associated to a significantly oxygen saturation decrease at T+3 and a significantly blood lactate increase at T+7 in the control calves. Moreover, pulmonary lesions were significantly less extensive than those in the control group. Taken together, this finding suggest that pharmacological modulation by carprofen of pulmonary inflammation after appearance of acute BPM clinical signs leads to calves’ health enhancement and reduces the extent of gross pneumonic lesion. [less ▲]

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See detailCaractériser un profil inflammatoire grâce à l'utilisation du microdamier
Ramery, Eve ULiege; Closset, Rodrigue; Bureau, Fabrice ULiege et al

Poster (2007)

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See detailEtude transcriptomique sur des cellules nucléées du sang et du lavage broncho-alveolaire chez des chevaux poussifs
Ramery, Eve ULiege

Master of advanced studies dissertation (2006)

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See detailIdentification de gènes d'intérêt pour le traitement du cheval poussif
Ramery, Eve ULiege; Closset, Rodrigue; Salinas, Emmanuelle et al

in Proceedings: AVEF, Versailles, France (2006)

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