Télédermatopathologie et dermatopathologie virtuelle en temps réel.
REGINSTER, Marie-Annick ; Pierard, Sébastien ; PIERARD, Gérald et al
in Dermatologie Actualité (2012), 129Detailed reference viewed: 25 (3 ULg)
The skin ivory spot. A possible indicator for skinfield photocarcinogenesis in recreational sunbed addicts.
QUATRESOOZ, Pascale ; FRANCHIMONT, Claudine ; PIERARD, Gérald
in International Journal of Environmental Research and Public Health (2012), 9Detailed reference viewed: 14 (1 ULg)
Smouldering malignant melanoma and metastatic dormancy: an update and review.
PIERARD, Gérald ; PIERARD-FRANCHIMONT, Claudine ; REGINSTER, Marie-Annick et al
in Dermatology Research and Practice (2012), 2012
The fund of knowledge regarding the versatility of presentation of MM metastases is still quite incomplete. The recent literature pertaining to the current understanding of the mechanisms underlying two ... [more ▼]
The fund of knowledge regarding the versatility of presentation of MM metastases is still quite incomplete. The recent literature pertaining to the current understanding of the mechanisms underlying two special features of MM metastasis is reviewed. On the one hand, a long disease-free interval (MM dormancy) may occur before the surge of overt metastases. On the other hand, the so-called MM smouldering phenomenon refers to the condition where regional metastases wax and wane for long periods of time on restricted skin regions. It is important to emphasize that local micrometastases often predict sentinel lymph node involvement but may not reflect progression of the primary MM to full-blown visceral metastatic competence. It is likely that a combination of factors impacts the versatile MM metastasic progression. Among the main factors, one has to mention the phenotypic heterogeneity and variability in the phenotype of MM cells, the presence of MM stem cells and MM cells engaged in an amplification proliferation pool, as well as the host immune response, and possibly the induction of a particular stromal structure and vascularity. [less ▲]Detailed reference viewed: 18 (4 ULg)
Molecular dermatopathology in malignant melanoma.
REGINSTER, Marie-Annick ; PIERARD-FRANCHIMONT, Claudine ; PIERARD, Gérald et al
in Dermatology Research and Practice (2012), 2012(684032), 1-6
At present, immunohistochemistry is taken for granted in the establishment of malignant melanoma (MM) diagnosis. In recent years, molecular diagnosis in dermatopathology has benefited from a vast array of ... [more ▼]
At present, immunohistochemistry is taken for granted in the establishment of malignant melanoma (MM) diagnosis. In recent years, molecular diagnosis in dermatopathology has benefited from a vast array of advances in the fields of genomics and proteomics. Sensitive techniques are available for detecting specific DNA and RNA sequences by molecular hybridization. This paper intends to update methods of molecular cytogenetics available as diagnostic adjuncts in the field of MM. Cytogenetics has highlighted the pathogenesis of atypical melanocytic neoplasms with emphasis on the activation of the mitogen-activated protein kinase (MAPK) signalling pathway during the initiation step of the neoplasms. 20 to 40% of MM families have mutations in the tumour suppressor gene p16 or CDKN2A. In addition, somatic mutations in p16, p53, BRAF, and cKIT are present in MM. Genome-wide scan analyses on MM indicate positive associations for genes involved in melanocytic naevi, but MM is likely caused by a variety of common low-penetrance genes. Molecular dermatopathology is expanding, and its use in the assessment of melanocytic neoplasms appears to be promising in the fields of research and diagnosis. Molecular dermatopathology will probably make its way to an increased number of diagnostic laboratories. The expected benefit should improve the patient management. This evolution points to a need for evolution in the training requirements and role of dermatopathologists. [less ▲]Detailed reference viewed: 17 (6 ULg)
Field melanin mapping of the hairless scalp.
PIERARD, Gérald ; FRANCHIMONT, Claudine ; Quatresooz, Pascale
in Skin Research & Technology (2011), 18(4), 431-5
BACKGROUND: Mottled subclinical melanoderma (MSM) is frequently seen on facial skin using the ultraviolet light enhanced visualization (ULEV) method. The corresponding aspect on the hairless scalp remains ... [more ▼]
BACKGROUND: Mottled subclinical melanoderma (MSM) is frequently seen on facial skin using the ultraviolet light enhanced visualization (ULEV) method. The corresponding aspect on the hairless scalp remains unknown. OBJECTIVE: To explore the field distribution of melanin on the scalp of fair-skinned Caucasian subjects. METHOD: The scalp was examined in 43 men with androgenic alopecia. The Visioscan((R)) camera provided the ULEV pictures. Another optical (Visioface((R)) Quick) device was used under white light illumination followed by colour contrast enhancement. This was reached after specific computer filtration of the cyan hue wavelengths. RESULTS: Under white light illumination, the scalp looked normal. MSM patterns were disclosed by both optical procedures as evenly scattered discrete patchy fields of hypermelanosis. The smaller rounded spots were restricted to the lips of the hair infundibula. Larger irregularly shaped spots predominated in the interfollicular areas. A few hypomelanotic spots were scattered over the scalp. CONCLUSION: The present observations based on dual optical methods possibly provide information about a patterned pathobiology of melanocytes on the scalp. The spotty MSM pattern looked similar to the reported aspects on the face. It somewhat resembled the widespread PUVA-induced lentiginosis. [less ▲]Detailed reference viewed: 11 (3 ULg)
Comment j'explore...le meli-melo des ichtyoses.
Hermanns-Lê, Trinh ; REGINSTER, Marie-Annick ; QUATRESOOZ, Pascale et al
in Revue Médicale de Liège (2011), 66(2), 102-8
Ichthyoses are hereditary and sometimes acquired diseases of keratinisation. They are heterogeneous according to their clinical and histopathological presentations, as well as to the nature of their ... [more ▼]
Ichthyoses are hereditary and sometimes acquired diseases of keratinisation. They are heterogeneous according to their clinical and histopathological presentations, as well as to the nature of their molecular and genetic alterations. We present the most frequent types of hereditary ichthyoses. [less ▲]Detailed reference viewed: 23 (2 ULg)
Hypomelanosis of Ito: pigmentary mosaicism with immature melanosome in keratinocytes.
; QUATRESOOZ, Pascale ; Hermanns-Lê, Trinh et al
in International Journal of Dermatology (2011), 50(10), 1234-9
BACKGROUND: Hypomelanosis of Ito is a rare genetic disorder characterized by whorled areas of hypomelanosis. The purpose of the present study was to revisit some aspects of Ito's hypomelanosis. METHODS ... [more ▼]
BACKGROUND: Hypomelanosis of Ito is a rare genetic disorder characterized by whorled areas of hypomelanosis. The purpose of the present study was to revisit some aspects of Ito's hypomelanosis. METHODS: Clinical observations included ultraviolet-light-enhanced visualization (ULEV) method. Histochemistry, immunohistochemistry, and electron microscopy were performed on biopsy samples from the hypopigmented areas and the surrounding skin. RESULTS: Both the ULEV and microscopic examinations revealed the heterogeneity of the pigmentation. Hypomelanosis was characterized by a reduction in melanosomes, both in melanocytes and keratinocytes. These organelles were immature and atypical, showing a weak tyrosinase immunoreactivity. Melanosome macroautophagy was prominent in keratinocytes. Some clusters of the same cells exhibited strong immunoreactivity for the Mac 387 antibody (Ca(2+) -dependent calprotectin). Ulex europaeus agglutinin-1 (UEA-1) decorated the superficial layers of the epidermis. Such features are typically found in functionally altered keratinocytes. A number of dermal cells exhibited intense phagocytic activity linked to lysozyme immunoreactivity. CONCLUSIONS: Both the melanosome depletion and macroautophagy of immature melanosomes in keratinocytes appeared to represent prominent aspects of hypomelanosis of Ito. In sum, Ito's hypomelanosis combines structural and functional changes affecting both the melanocytes and keratinocytes in the skin. [less ▲]Detailed reference viewed: 29 (2 ULg)
Traitement par anti-TNF-alpha et cancers de la peau
Pierard, Claudine ; Pierard, Gérald ; Quatresooz, Pascale et al
in ONCO-HEMATO (2011), 5Detailed reference viewed: 17 (2 ULg)
''Malignant melanoma microecosystem'': Immunohistopathological insights into the stromal cell phenotype (Review)
QUATRESOOZ, Pascale ; REGINSTER, Marie-Annick ; PIERARD, Gérald
in Experimental and Therapeutic Medicine (2011), 2Detailed reference viewed: 25 (9 ULg)
Traitement par anti-TNF-alpha et cancers de la peau.
FRANCHIMONT, Claudine ; PIERARD, Gérald ; QUATRESOOZ, Pascale et al
in Onco-Hémato (2011), 5Detailed reference viewed: 15 (1 ULg)
Le cornéocyte immature et fragile, un phénotype marqueur d'un environnement hostile.
QUATRESOOZ, Pascale ; PIERARD, Gérald ; FRANCHIMONT, Claudine
in Dermatologie Actualité (2011), 126Detailed reference viewed: 10 (0 ULg)
Predictive methods exploring sensory irritation to surfactant-based products.
Pierard, Gérald ; ; QUATRESOOZ, Pascale
in Household and Personal Care (2011), 7Detailed reference viewed: 15 (1 ULg)
Identification d'une dermatose provoquée par la lumière
PIERARD-FRANCHIMONT, Claudine ; CAUCANAS, Marie ; QUATRESOOZ, Pascale et al
in Revue Médicale de Liège (2011), 66Detailed reference viewed: 20 (0 ULg)
Revisiting cutaneous adverse reactions to pemetrexed
PIERARD-FRANCHIMONT, Claudine ; QUATRESOOZ, Pascale ; REGINSTER, Marie-Annick et al
in Oncology Letters (2011), (2), 769-772Detailed reference viewed: 15 (2 ULg)
Micropapules paroxysmales prurigineuses tronculaires.
CAUCANAS, Marie ; PIERARD, Gérald ; FRANCHIMONT, Claudine et al
in Dermatologie Actualité (2011), 127Detailed reference viewed: 26 (0 ULg)
La peau du patient sous chimiothérapie anticancéreuse.
PIERARD, Gérald ; PAQUET, Philippe ; FRANCHIMONT, Claudine et al
in Dermatologie Actualité (2011), 124
Des effets cutanés indésirables peuvent survenir lors de chimiothérapies anticancéreuses. La morbidité peut être handicapante à des degrés divers. Certaines manifestations sont graves comme celles ... [more ▼]
Des effets cutanés indésirables peuvent survenir lors de chimiothérapies anticancéreuses. La morbidité peut être handicapante à des degrés divers. Certaines manifestations sont graves comme celles rencontrées lors de nécroses épithéliales et/ou vasculaires étendues. D’autres sont spécifiques à l’agent administré. Tel est le cas pour des éruptions acnéiformes induites par les antagonistes de l’EGFR. Dans la grande majorité des cas, les altérations de la structure de la couche cornée et de ses fonctions se résument seulement à un état de xérose. Parfois aussi, les altérations ne se décèlent qu’à l’examen histopathologique sans répercussion clinique manifeste. Pour chaque patient concerné, il convient d’identifier avec précision les effets indésirables de la chimiothérapie anticancéreuse afin de choisir les moyens les plus adéquats pour prévenir leur aggravation et les traiter au mieux. L’apport de l’examen dermatopathologique standard et fonctionnel est précieux au stade du diagnostic. [less ▲]Detailed reference viewed: 53 (4 ULg)
Malignant melanoma: from cell kinetics to micrometastases.
QUATRESOOZ, Pascale ; PIERARD, Gérald
in American Journal of Clinical Dermatology (2011), 12(2), 77-86
Malignant melanoma (MM) micrometastases are basically seen in three locations inside the peritumoral dermis. They are localized (i) inside the interstitial sector of the dermal stroma; (ii) abutted to the ... [more ▼]
Malignant melanoma (MM) micrometastases are basically seen in three locations inside the peritumoral dermis. They are localized (i) inside the interstitial sector of the dermal stroma; (ii) abutted to the external surface of the microvasculature; and (iii) more rarely present inside vascular channels. Single-cell and paucicellular micrometastases may be disclosed using immunohistochemistry even in the absence of larger microsatellites, which represent micronodular nests of metastatic cells. The presence of microsatellites is frequently tied to markers of MM aggressiveness including thickness and the Ki-67 index. Micrometastases may be present in the same conditions, but even as early as thin MM showing a small growth fraction. Microsatellites as well as micrometastases appear to predict locoregional extension and decreased relapse-free interval, but not distant metastasis and overall survival. These considerations have implications for patient care since patients with microsatellites and micrometastases are now included in the clinical stage III category of the disease. Their implication as a prognostic factor is not fully dependent on or linked to other markers of MM aggressiveness. [less ▲]Detailed reference viewed: 14 (3 ULg)
Dynamics of skin barrier repair following preconditioning by a biotechnology-driven extract from samphire (Crithmum maritimum) stem cells.
CAUCANAS, Marie ; ; Pierard, Gérald et al
in Journal of Cosmetic Dermatology (2011), 10(4), 288-93
Background With aging, the barrier repair kinetics following any weakening of the epidermal permeability barrier function is commonly slowed down. Objective To assess the recovery rate of the epidermal ... [more ▼]
Background With aging, the barrier repair kinetics following any weakening of the epidermal permeability barrier function is commonly slowed down. Objective To assess the recovery rate of the epidermal permeability barrier function following controlled stripping and applications of samphire and control formulations. Method In 12 healthy subjects older than 50 years, controlled stratum corneum (SC) strippings were used to increase the transepidermal water loss (TEWL) just above 15 g/m(2) /h. This procedure followed a 14-day skin preconditioning by daily applications of formulations enriched or not with a samphire (Crithmum maritimum) biomass. An untreated skin site served as control. The epidermal permeability repair kinetics was assessed for 14 days by daily measurements of both TEWL and the colorimetric value a*. Results A rapid (96 h) recovery to lower TEWL values was obtained at each of the samphire-preconditioned sites (0.1% serum, 0.05% cream, the serum-cream association, and 0.5% silicone oil). This process was significantly (P < 0.001) faster than that on both the placebo-preconditioned (silicone oil) and the untreated sites. No adverse inflammatory and sensory reactions were recorded. At the sites preconditioned by samphire formulations, the SC moisture (capacitance) was higher at completion of the study compared to inclusion. Conclusions The present experimental pilot study brings some clues supporting a beneficial boosting effect of samphire cell biomass on the kinetics of epidermal permeability barrier repair. [less ▲]Detailed reference viewed: 15 (2 ULg)