References of "Portetelle, Daniel"
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See detailIdentification of bacteria community associated with earthworm gut
Lemtiri, Aboulkacem ULg; Alabi, Taofic; Bodson, Bernard ULg et al

Poster (2012, July 26)

The role of earthworms in soil fertility and transformation of organic waste was regulary cited to be of first importance. Associated to these macro-invertebrates, a large diversity of micro-orgnisms are ... [more ▼]

The role of earthworms in soil fertility and transformation of organic waste was regulary cited to be of first importance. Associated to these macro-invertebrates, a large diversity of micro-orgnisms are found indirectly in their closed environment or directly in their gut. Functional aspects of these interactions and symbiosis in relation with soil characteristics and fertility rates are poorly developed. Here, the micro-organisms diversity and potential related functions of earthworm gut were investigated using a proteiomic approach for both protein and micro-organism identifications. Microbial community investigation was detected by proteomic approach based on bidimensional electrophoresis coupled with mass spectrometry using Matrix Assisted Laser Desorption Ionisation – time of flight (Maldi-Tof). Diversity of gut associated bacterial communities was discussed. Indeed, application of particular crop production practices such as crop residue management at the field level could regulate the gut bacterial communities in earthworm but also microbials in soils. Agricultural systems had to consider the microbial and associated organisms in the soil to enhance fertlility and crop production in sustainable ways. [less ▲]

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See detailImpact of soil management on earthworm diversity according to differential plowing and plant residue incorporation
Lemtiri, Aboulkacem ULg; Alabi, Taofic; Zirbes, Lara ULg et al

Poster (2012, July 02)

Earthworms are largely distributed in terrestrial ecosystems and their abundance and diversity in soils are significantly affected by biotic (macro- and micro-organisms) and abiotic factors: soil ... [more ▼]

Earthworms are largely distributed in terrestrial ecosystems and their abundance and diversity in soils are significantly affected by biotic (macro- and micro-organisms) and abiotic factors: soil properties (pH, texture, structure…); agricultural management system and climate change. Here, tillage effect of earthworm population combined with crops residual management was investigated and correlated with soils properties. From wheat experimental field plots, the diversity of earthworm according to the field crop management was assessed. Application of particular crop production practices such as the integration of different levels of crop residues, diverse parts of wheat straws, at the field level regulate earthworm diversity and population abundance. Indeed, tillage reduced earthworm population with a 35% rate also corresponding to changes in soil properties. Agricultural practices had to be adapted to include consideration on macro-invertebrate abundance and diversity to maintain efficient soil fertility and allow sustainable crop production [less ▲]

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See detailSelection and cultivation of hydrolytic microorganisms extracted from the digestive tract of the termite Reticulitermes santonensis (3DV.1.55)
Tarayre, Cédric ULg; Bauwens, Julien ULg; Matteotti, Christel ULg et al

Poster (2012, June 21)

Biofuel production can be based on the use of fermentable substrates issued from the hydrolysis of lignocellulosic biomass stemming from agricultural residues and by-products. However, such substrates are ... [more ▼]

Biofuel production can be based on the use of fermentable substrates issued from the hydrolysis of lignocellulosic biomass stemming from agricultural residues and by-products. However, such substrates are not easy to degrade. Enzymes (cellulases, xylanases, etc.) can be used for this purpose and pre-treatments can increase their action by providing more available extremities. The digestive tract of the termite Reticulitermes santonensis contains various microorganisms (bacteria, molds, protists) able to degrade the wood components. These microorganisms act as consortia, leading to a better hydrolysis than in the cow rumen. Our purpose is the isolation of microorganisms from termite guts in order to evaluate their potential for hydrolysis of lignocellulosic materials. This approach led us to isolate and to study a bacteria (Bacillus sp.) displaying a xylanase activity, a mold (Aspergillus sp.) displaying a cellulase activity and a chrysophyte (protist) displaying an amylase activity. [less ▲]

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See detailIn vitro determination of prebiotic potential of sugar beet pulp extracted candidates and influence of production method
François, Emmanuelle ULg; Goffin, Dorothée ULg; combo, Agnan Marie-Michelle et al

Poster (2012, June 12)

Various prebiotic hydrolysates were produced starting from sugar beet pulp (SBP) using alternative solution to traditional acidic hydolysis. SBP pectin was first extracted by acid extraction and ethanol ... [more ▼]

Various prebiotic hydrolysates were produced starting from sugar beet pulp (SBP) using alternative solution to traditional acidic hydolysis. SBP pectin was first extracted by acid extraction and ethanol precipitation. Then two technics are used to produced pectic oligosaccharides (POS) : enzymatic hydrolysis (EnzPOS : Rapidase Smart (DSM) ; 50°C ; pH 5) and microwaves-assisted hydrolysis (MW1POS : 104°C/10min/pH 7; MW2POS : 110°C/15min/pH 7). Structural characterization of fractions gave rise to different structural profiles between the four products. Moreover, fermentation parameters obtained in-vitro (A, B, Rmax and Tmax) were also characteristic of the fractions and in favor of a tight relationship between POS structure and POS function. Finally, to avoid solvent use, POS production could be envisaged directly on SBP. Avoiding the step of acidic extraction will allow to meet the green chemistry principles. [less ▲]

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See detailUse of medium without reducing agent for in vitro fermentation studies by bacteria isolated from pig intestine
POELAERT, Christine ULg; Boudry, Christelle ULg; Portetelle, Daniel ULg et al

in XII International Symposium on Digestive Physiology of Pigs (2012, May)

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See detailScaphandre La science rencontre l'art: L'art
Haubruge, Eric ULg; Bay, Daniel ULg; Semal, Jean et al

in Haubruge, Eric; Bay, Daniel; Semal, Jean (Eds.) Scaphandre La science rencontre l'art (2012)

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See detailInteraction network of antimicrobial peptides of Arabidopsis thaliana, based on hith-throughput yeast two-hybrid screening
Damon, Coralie ULg; Dmitrieva, Joelia Borisnova; Muhovski, Yordan et al

in Plant Physiology & Biochemistry (2012)

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See detailChanges in termites feeding diets for gut micro-organisms selection and further cultivation
Bauwens, Julien ULg; Brasseur, Catherine ULg; Matteotti, Christel ULg et al

Poster (2011, October 02)

Termites gut may overcome important dietary perturbations, initial diversity acting as key point buffering effects on host, although termites possess their own enzymatic system. Some artificial diets ... [more ▼]

Termites gut may overcome important dietary perturbations, initial diversity acting as key point buffering effects on host, although termites possess their own enzymatic system. Some artificial diets permitted a simplification of the lower termites gut symbiotic system, which could be used as first step in symbionts isolation and cultivation. Preliminary assay of cultivation actually gave encouraging results. Proteomic proved to be suitable tool to investigate such a complex system. Nevertheless, for some symbionts very few genes are sequenced, which should lead to more targeted proteomic studies. Protein chromatography will allow to split up the proteome and more accurate analysis. [less ▲]

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See detailShifts in microbial population fermenting contrasting indigestible carbohydrate sources measured in the intestines of pigs and using an in vitro model of the pig gastro-intestinal tract
Boudry, Christelle ULg; Bindelle, Jérôme ULg; Portetelle, Daniel ULg et al

in International Scientific Conference on probiotics and prebiotics - IPC 2011 Conference Proceedings (2011, June)

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