References of "Portetelle, Daniel"
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See detailUse of medium without reducing agent for in vitro fermentation studies by bacteria isolated from pig intestine
POELAERT, Christine ULg; Boudry, Christelle ULg; Portetelle, Daniel ULg et al

in Journal of Animal Science (2012, December), 90(Supplement 4), 387-389

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See detailUtilisation des termites comme source de microorganismes dans la filière de production du bioéthanol de seconde génération
Tarayre, Cédric ULg; Bauwens, Julien ULg; Brasseur, Catherine ULg et al

Poster (2012, November 14)

Les termites abritent une microflore symbiotique qui intervient dans la dégradation des fibres constitutives du bois, synthétisant des enzymes capables d’hydrolyser ses composants. Les sucres ... [more ▼]

Les termites abritent une microflore symbiotique qui intervient dans la dégradation des fibres constitutives du bois, synthétisant des enzymes capables d’hydrolyser ses composants. Les sucres fermentescibles libérés suite à cette hydrolyse sont utilisables dans le cadre de la production du bioéthanol de seconde génération. [less ▲]

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See detailSTUDY OF BACTERIAL DIVERSITY IN AN AGRICULTURAL SOIL
Stroobants, Aurore ULg; Bodson, Bernard ULg; Portetelle, Daniel ULg et al

Poster (2012, August 19)

Bacterial growth in soil is dependent on soil characteristics. In this experiment, we have studied the evolution of bacterial diversity during a winter wheat crop and the impacts caused by the tillage and ... [more ▼]

Bacterial growth in soil is dependent on soil characteristics. In this experiment, we have studied the evolution of bacterial diversity during a winter wheat crop and the impacts caused by the tillage and residue incorporation. Three growth stages of wheat was chosen for this work : germination, tillering and booting. The analyse of bacterial diversity in these conditions was performed by the Next Generation Sequencing technology. Results obtained by this method indicate that the soil is composed, in average, by 38,02 (±4,81)% Proteobacteria; 19,71(±3,88)% Actinobacteria; 7,77(±1,44)% Firmicutes; 6,94(±1,58)% Fibrobacteres/Acidobacteria group; 5(±3,21)% Bacteroidetes/Chlorobi group; 3,89(±1,36)% Chloroflexi; 2,96(±0,67)% Planctomycetes; 2,87(±1,58)% Verrucomicrobia; 1,42(±0,41)% Cyanobacteria and 15,38(±2,64)% others. The tillage influences mostly the Deltaproteobacteria, Gammaproteobacteria, Actinobacteridae, Bacteroidetes/Chlorobi group and Verrucomicrobia. Residue incorporation has an impact on Betaproteobacteria, Gammaproteobacteria, Actinobacteridae, Acidimicrobidae, Firmicutes and Bacteroidetes/Chlorobi group. The wheat growth stages affect especially Betaproteobacteria, Deltaproteobacteria, Actinomycetales, Acidibacteria, Fibrobacteres and Bacillales. The results will be presented and discussed on the poster. [less ▲]

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See detailIMPACT OF DEPTH AND SOIL COMPACTION ON BACTERIAL DIVERSITY IN SOIL
Stroobants, Aurore ULg; Degrune, Florine; Olivier, Claire et al

Poster (2012, August 19)

Bacteria are the most abundant and diverse microorganisms in soils. The amount of bacteria in soils can reach 10^10 cells per gram of soil. These organisms are involved in various processes in ... [more ▼]

Bacteria are the most abundant and diverse microorganisms in soils. The amount of bacteria in soils can reach 10^10 cells per gram of soil. These organisms are involved in various processes in agroecosystems such as nutrient cycling, contributing to plant nutrition, plant health and soil structure. The knowledge about this diversity is limited because only one percent of these organisms can be cultured by laboratory methods. During the last decades, many molecular-based techniques have been developed to assess the diversity of bacterial communities. The aim of this study was to determine the quantity and diversity of bacteria in two agricultural soils with differents soil management practices (tillage and no tillage) at different depths (10, 30 and 45 centimeters) and different compaction levels (high and low). Quantity was evaluated by real time PCR and diversity was analysed by the DGGE (Denaturing Gradient Gel Electrophoresis) technique. The results show that soil management has an impact on bacterial quantity at 45 centimeters and quantity is higher in till soil. Compaction level affects the bacterial quantity in till soil, quantity is higher in low compaction. And finally, depth influences the bacterial quantity in till and no till soil. In both soils, quantity decreases with the depth. The results will be presented and discussed on the poster. [less ▲]

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See detailTermites artificially-fed on unusual diet and resulting enzymatic switches
Bauwens, Julien ULg; Tarayre, Cédric ULg; Matteotti, Christel et al

Poster (2012, August)

Wood-feeding termites as Reticulitermes santonensis generally feed on cellulose, hemicelluloses and lignin. However, these opportunistic insects are also able to degrade other carbohydrates, such as ... [more ▼]

Wood-feeding termites as Reticulitermes santonensis generally feed on cellulose, hemicelluloses and lignin. However, these opportunistic insects are also able to degrade other carbohydrates, such as starch. The production of putative endogenous α - amylase has been previously shown in R. flavipes, as the disappearance of the major symbiotic flagellates from the hindgut. Here, we compared enzymatic activities (CMCase, MCCase, xylanase, amylase, α- and β-glucosidase) between different fractions of the digestive tract of starch-, cellulose-, and wood-fed termites. Main compounds of the artificial diets, namely starch or MCC, resulted in differential enzymatic activity. Even the substitution of wood by artificial diets itself seemed to induce changes in enzymatic activities, regardless of the main substrate in the diet, as we observed strong midgut α-glucosidase activity only for artificially-fed termites. Preliminary assays to isolate and characterize enzymes were performed using proteomic methods. [less ▲]

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See detailIdentification of bacteria community associated with earthworm gut
Lemtiri, Aboulkacem ULg; Alabi, Taofic; Bodson, Bernard ULg et al

Poster (2012, July 26)

The role of earthworms in soil fertility and transformation of organic waste was regulary cited to be of first importance. Associated to these macro-invertebrates, a large diversity of micro-orgnisms are ... [more ▼]

The role of earthworms in soil fertility and transformation of organic waste was regulary cited to be of first importance. Associated to these macro-invertebrates, a large diversity of micro-orgnisms are found indirectly in their closed environment or directly in their gut. Functional aspects of these interactions and symbiosis in relation with soil characteristics and fertility rates are poorly developed. Here, the micro-organisms diversity and potential related functions of earthworm gut were investigated using a proteiomic approach for both protein and micro-organism identifications. Microbial community investigation was detected by proteomic approach based on bidimensional electrophoresis coupled with mass spectrometry using Matrix Assisted Laser Desorption Ionisation – time of flight (Maldi-Tof). Diversity of gut associated bacterial communities was discussed. Indeed, application of particular crop production practices such as crop residue management at the field level could regulate the gut bacterial communities in earthworm but also microbials in soils. Agricultural systems had to consider the microbial and associated organisms in the soil to enhance fertlility and crop production in sustainable ways. [less ▲]

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See detailImpact of soil management on earthworm diversity according to differential plowing and plant residue incorporation
Lemtiri, Aboulkacem ULg; Alabi, Taofic; Zirbes, Lara ULg et al

Poster (2012, July 02)

Earthworms are largely distributed in terrestrial ecosystems and their abundance and diversity in soils are significantly affected by biotic (macro- and micro-organisms) and abiotic factors: soil ... [more ▼]

Earthworms are largely distributed in terrestrial ecosystems and their abundance and diversity in soils are significantly affected by biotic (macro- and micro-organisms) and abiotic factors: soil properties (pH, texture, structure…); agricultural management system and climate change. Here, tillage effect of earthworm population combined with crops residual management was investigated and correlated with soils properties. From wheat experimental field plots, the diversity of earthworm according to the field crop management was assessed. Application of particular crop production practices such as the integration of different levels of crop residues, diverse parts of wheat straws, at the field level regulate earthworm diversity and population abundance. Indeed, tillage reduced earthworm population with a 35% rate also corresponding to changes in soil properties. Agricultural practices had to be adapted to include consideration on macro-invertebrate abundance and diversity to maintain efficient soil fertility and allow sustainable crop production [less ▲]

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See detailSelection and cultivation of hydrolytic microorganisms extracted from the digestive tract of the termite Reticulitermes santonensis (3DV.1.55)
Tarayre, Cédric ULg; Bauwens, Julien ULg; Matteotti, Christel ULg et al

Poster (2012, June 21)

Biofuel production can be based on the use of fermentable substrates issued from the hydrolysis of lignocellulosic biomass stemming from agricultural residues and by-products. However, such substrates are ... [more ▼]

Biofuel production can be based on the use of fermentable substrates issued from the hydrolysis of lignocellulosic biomass stemming from agricultural residues and by-products. However, such substrates are not easy to degrade. Enzymes (cellulases, xylanases, etc.) can be used for this purpose and pre-treatments can increase their action by providing more available extremities. The digestive tract of the termite Reticulitermes santonensis contains various microorganisms (bacteria, molds, protists) able to degrade the wood components. These microorganisms act as consortia, leading to a better hydrolysis than in the cow rumen. Our purpose is the isolation of microorganisms from termite guts in order to evaluate their potential for hydrolysis of lignocellulosic materials. This approach led us to isolate and to study a bacteria (Bacillus sp.) displaying a xylanase activity, a mold (Aspergillus sp.) displaying a cellulase activity and a chrysophyte (protist) displaying an amylase activity. [less ▲]

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See detailIn vitro determination of prebiotic potential of sugar beet pulp extracted candidates and influence of production method
François, Emmanuelle ULg; Goffin, Dorothée ULg; combo, Agnan Marie-Michelle et al

Poster (2012, June 12)

Various prebiotic hydrolysates were produced starting from sugar beet pulp (SBP) using alternative solution to traditional acidic hydolysis. SBP pectin was first extracted by acid extraction and ethanol ... [more ▼]

Various prebiotic hydrolysates were produced starting from sugar beet pulp (SBP) using alternative solution to traditional acidic hydolysis. SBP pectin was first extracted by acid extraction and ethanol precipitation. Then two technics are used to produced pectic oligosaccharides (POS) : enzymatic hydrolysis (EnzPOS : Rapidase Smart (DSM) ; 50°C ; pH 5) and microwaves-assisted hydrolysis (MW1POS : 104°C/10min/pH 7; MW2POS : 110°C/15min/pH 7). Structural characterization of fractions gave rise to different structural profiles between the four products. Moreover, fermentation parameters obtained in-vitro (A, B, Rmax and Tmax) were also characteristic of the fractions and in favor of a tight relationship between POS structure and POS function. Finally, to avoid solvent use, POS production could be envisaged directly on SBP. Avoiding the step of acidic extraction will allow to meet the green chemistry principles. [less ▲]

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See detailUse of medium without reducing agent for in vitro fermentation studies by bacteria isolated from pig intestine
POELAERT, Christine ULg; Boudry, Christelle ULg; Portetelle, Daniel ULg et al

in XII International Symposium on Digestive Physiology of Pigs (2012, May)

Detailed reference viewed: 38 (10 ULg)