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See detailIsolation of insecticidal proteins within the pea seeds (Pisum sativum L.)
Cuartero Diaz, Gaëtan; Francis, Frédéric ULg; Portetelle, Daniel ULg et al

Poster (2006, October)

Consequently with the pressure exerted by chemical pesticides on environment, and the awakening of politics, the demand for bio-pesticides is increasing. Nevertheless, supply is not sufficient, and ... [more ▼]

Consequently with the pressure exerted by chemical pesticides on environment, and the awakening of politics, the demand for bio-pesticides is increasing. Nevertheless, supply is not sufficient, and moreover those products are not competing enough. In this context, the aim of this research is to set up a biological insecticide, which is economic, with vegetal proteins resulting from alimentary industry, here the pea, Pisum sativum L.. A group of proteins, which is quite easy to highlight, is present in relatively important proportions (2%) in pea seeds, it’s the lectins class. Insecticidal effects of lectins from different organisms have already been proved. Indeed, by binding to membrane glycosyl groups of digestive tract cells, lectins can be very toxic for a lot of insects. Thus initially we focus our investigations on Pisum sativum lectins (PSL). First, PSL have been localised within the industrial process among different extraction juices. Then, a chromatography has been performed on the selected juice with FPLC technology. Although the matrix used for this chromatography, sephadex G75, is a banal bed for gel filtration, it is in this case a real combination between classical gel filtration and affinity chromatography. Indeed due the particular properties of lectins, they fixed carbonyl group of the bed and have to be eluted after the filtration part with a solution of glucose. Then the collected fractions corresponding to UV-peaks on the chromatogram were separated by electrophoresis 2D and identified by mass spectrometry (ESI MS/MS) coupled with data bank investigations. Secondly bioassays using artificial diets have been developed on Myzus persicae in the aim to study the aphicid effects of theses fractions with rm and LC50.These estimators show significant mortality rates but also change in the fecundity and in the development of nymph. [less ▲]

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See detailValorisation du colostrum bovin. Rapport Scientifique et Technique Final
Halleux, Caroline; Boudry, Christelle ULg; Portetelle, Daniel ULg et al

Report (2006)

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See detailMapping and polymorphism of bovine ghreline gene
Colinet, Frédéric ULg; Eggen, André; Gengler, Nicolas ULg et al

in Proceedings of the 30th International Conference on Animal Genetics ISAG 2006 - Biodiversity, the future pass through preservation (2006, August)

Bovine ghrelin, a 27-amino-acid peptide has been identified in bovine oxyntic glands of the abomasum. It is an endogenous growth hormone secretagogue. Total mRNA was extracted from abomasum and complete ... [more ▼]

Bovine ghrelin, a 27-amino-acid peptide has been identified in bovine oxyntic glands of the abomasum. It is an endogenous growth hormone secretagogue. Total mRNA was extracted from abomasum and complete ghrelin mRNA was sequenced by rapid amplification of cDNA ends. The gene contains five exons and four introns with a short noncoding first exon of 17 bp similar to mouse and human ghrelin gene. Using a radiation hybrid panel, the gene was mapped to chromosome 22 near microsatellite markers UWCA49, BM4102, BMS1932, BM2613 and URB035 with good LOD Score. Some studies detected different QTLs near these markers like for milk fat percent, milk protein percent and somatic cell score. So, it would be interesting to study the polymorphism on the bovine ghrelin gene. Screening for polymorphisms in the five exons and the introns II and IV on ten Belgian Blue bulls, ten Holsteins bulls and ten Limousin bulls revealed a total of three single nucleotide polymorphisms. In order to evaluate if ghrelin could be involved in genetic variation for milk fat percent, milk protein percent and somatic cell score an association study between SNPs on ghrelin gene and these traits could be performed in a major cattle population. [less ▲]

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See detailMapping and polymorphism of bovine ghreline gene
Colinet, Frédéric ULg; Eggen, André; Halleux, Caroline et al

Poster (2006, August)

Bovine ghrelin, a 27-amino-acid peptide has been identified in bovine oxyntic glands of the abomasum. It is an endogenous growth hormone secretagogue. Total mRNA was extracted from abomasum and complete ... [more ▼]

Bovine ghrelin, a 27-amino-acid peptide has been identified in bovine oxyntic glands of the abomasum. It is an endogenous growth hormone secretagogue. Total mRNA was extracted from abomasum and complete ghrelin mRNA was sequenced by rapid amplification of cDNA ends. The gene contains five exons and four introns with a short noncoding first exon of 17 bp similar to mouse and human ghrelin gene. Using a radiation hybrid panel, the gene was mapped to chromosome 22 near microsatellite markers UWCA49, BM4102, BMS1932, BM2613 and URB035 with good LOD Score. Some studies detected different QTLs near these markers like for milk fat percent, milk protein percent and somatic cell score. So, it would be interesting to study the polymorphism on the bovine ghrelin gene. Screening for polymorphisms in the five exons and the introns II and IV on ten Belgian Blue bulls, ten Holsteins bulls and ten Limousin bulls revealed a total of three single nucleotide polymorphisms. In order to evaluate if ghrelin could be involved in genetic variation for milk fat percent, milk protein percent and somatic cell score an association study between SNPs on ghrelin gene and these traits could be performed in a major cattle population. [less ▲]

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See detailProduction and characterization of monoclonal and polyclonal antibodies against Scd14
Halleux, Caroline; Boudry, Christelle ULg; Thewis, André ULg et al

Poster (2006, May 17)

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See detailEffect Of Bovine Colostrum Supplementation On Cytokine mRNA Expression In Weaned Piglets
Boudry, Christelle ULg; Buldgen, André; Portetelle, Daniel ULg et al

in 10th International symposium on digestive physiology in pigs (2006, May)

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See detailValorisation du colostrum bovin. Rapport Scientifique et technique. Année 3.
Halleux, Caroline; Boudry, Christelle ULg; Portetelle, Daniel ULg et al

Report (2006)

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See detailQuantification of Bifidobacterium spp. and Lactobacillus spp. in rat fecal samples by real-time PCR.
Delroisse, Jean-Marc; Boulvin, Anne-Lise; Parmentier, Isabelle et al

in Microbiological research (2006), (5),

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See detailThe Tre2 Oncoprotein, Implicated In Ewing'S Sarcoma, Interacts With Two Components Of The Cytoskeleton
Dechamps, C.; Bach, S.; Portetelle, Daniel ULg et al

in Biotechnology Letters (2006), 28(4),

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See detailCharacterisation of bio-insecticide proteins in industrial residues from pea seeds (Pisum sativum L.)
Cuertero Diaz, Gaetan; Colinet, Frederic; Portetelle, Daniel ULg et al

in Abstract book (2006)

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See detailEffect of a bovine colostrum supplementation in piglet diet at weaning on growth performances, food ingestion and faecal E. coli concentrations.
Boudry, Christelle ULg; Didderen, Isabelle; Wavreille, José et al

in Proceedings of the British Society of Animal Science (2005, April)

Bovine colostrum supplementation in piglets diet stimulates food intake and reduces the faecal E. coli population during the first week after weaning, inducing a liveweight gain increase. A reduction of ... [more ▼]

Bovine colostrum supplementation in piglets diet stimulates food intake and reduces the faecal E. coli population during the first week after weaning, inducing a liveweight gain increase. A reduction of the food conversion ratio was also observed, suggesting a better nutrient assimilation by the piglets. [less ▲]

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See detailValorisation du colostrum bovin. Rapport Scientifique et technique. Année 2.
Halleux, Caroline; Boudry, Christelle ULg; Portetelle, Daniel ULg et al

Report (2005)

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See detailEffet du colostrum bovin administré à des porcelets au sevrage sur la morphologie de la paroi intestinale et sur le système immunitaire
Boudry, Christelle ULg; Dalle, Marie-Julie; Halleux, Caroline et al

in 37èmes journées de la recherche porcine (2005, February)

Two groups (A and B) of 6 piglets, weaned at 21 days (D0), received ad libitum the same diet during three weeks. The piglets of group A were not submitted to a colostrum supplementation while those of the ... [more ▼]

Two groups (A and B) of 6 piglets, weaned at 21 days (D0), received ad libitum the same diet during three weeks. The piglets of group A were not submitted to a colostrum supplementation while those of the group B received daily and orally 5 g of bovine colostrum. Each week, piglets were weighed and blood was taken on each one of them. One piglet of each group was sacrified on D0 and the rest of the animals on D21. Mesenteric lymph nodes (ML), iléal Peyer patches (iPP), jejunum and spleen biopsies were taken on each piglet before death. The haematological values and the intestinal histo-morphological data between the two groups were not significantly different, but FACS analyses on the lymphocytes in the biopsies and the determination of stimulation index suggested a significative effect of colostrum on the lymphocytes populations, with, in the ML, an increase of the LB population (14 % vs 28 %, P < 0,05) and, in the iPP, a reduction of the LB's (54,99 % vs 8,44 %, P < 0,01) with an increase of the LT cells (16,87 % vs 72,00 %, P < 0,001). An increase of the lymphocyte stimulation index from piglets receiving colostrum was observed the second day of in vitro stimulation with colostrum. In brief, the answer of the immunity system to bovine colostrum seems to be restricted to the intestinal immunity system and is characterised by an activation, a multiplication and a modification of the lymphocytes ratios. [less ▲]

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See detailContribution de la biologie moléculaire au progrès de l'élevage.
Renaville, Robert ULg; Georges, Michel ULg; Smal, Caroline et al

Conference (2005, January 26)

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See detailPCR detection of animal by-products in feeds (WP4)
Brambilla, G.; Fumiere, Olivier; Aarts, H. J. M. et al

in Stratfeed - Strategies and methods to detect and quantify mammalian tissues in feedingstuffs. (2005)

Detailed reference viewed: 10 (4 ULg)