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See detailEffect Of Bovine Colostrum Supplementation On Cytokine mRNA Expression In Weaned Piglets
Boudry, Christelle ULg; Buldgen, André; Portetelle, Daniel ULg et al

in Livestock Science (2007), 108(1-3), 295-298

The aim of this study was to evaluate the influence of a bovine colostrum supplementation on the immune Th1/Th2 response in weaned piglets. After weaning at 21 days, 21 piglets were fed daily ad libitum ... [more ▼]

The aim of this study was to evaluate the influence of a bovine colostrum supplementation on the immune Th1/Th2 response in weaned piglets. After weaning at 21 days, 21 piglets were fed daily ad libitum with a starter diet supplemented for 3 weeks with 0, 1 or 5 g of bovine colostrum. Growth performances were measured weekly. Spleen and gut-associated lymphoid tissues (GALT) (ileal Peyer’s patch (iPP), jejunum wall (JW) and mesenteric lymph nodes (MLN)) biopsies were collected on each piglet after the 3 weeks treatment and analyzed for their cytokine mRNA expression (IL-2, IL-4, IL-10, IL-12 and IFN) by RT-PCR. Growth performances were not affected by bovine colostrum (P > 0.05). In the colostrum groups, the iPP cells showed higher IL-4, IL-10 and IL-12 cytokine levels (P < 0,05), associated with a decrease of IFN-γ (P < 0,01) and no effect on IL-2 production (P > 0,05). IL-10, IL-2 and IL-12 productions increased (P < 0,05) in MLN, with no effect on IFN-γ and IL-4 levels (P > 0,05). The IL-12 production was also increased (P < 0,01) in JW cells with no modification for the other cytokine. No modification of cytokine production was observed in the spleen (P > 0,05). These results suggest an immunomodulatory effect of bovine colostrum on the GALT which answered by producing, at different levels, both Th1 pro-inflammatory cytokines - IL-2, IFN-γ and IL-12 - and Th2 anti-inflammatory cytokines - IL-4 and IL-10. This bipolarity of the Th1/Th2 response protects the weaned piglets from both allergic (food) as well as infectious (pathogens) diseases. [less ▲]

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See detailLipase-catalyzed interesterification of butterfat with rapeseed oil: new approaches for the monitoring of the reaction.
Hanon, Emilien ULg; Aguedo, Mario ULg; Danthine, Sabine ULg et al

Poster (2007)

Butterfat (BF) is one main source of diet fats. However, it has been less and less well perceived due to its poor spreadability when refrigerated and cholesterol and saturated fatty acids, promoters of ... [more ▼]

Butterfat (BF) is one main source of diet fats. However, it has been less and less well perceived due to its poor spreadability when refrigerated and cholesterol and saturated fatty acids, promoters of coronary heart diseases. Thus, consumer’s demand for healthy palatable fat spreads with good development of modified butter-based spreads. One ordinary method used by manufacturers for such modifications is enzymatic interesterification of a lipase to restructure triacylglycerides (TAG), i.e. to induce the exchange of fatty acid residues amongst glycerol backbones. This leads to changes in TAG species and in physical properties of the fat, namely in solid fat content (SFC) and in melting profile. Rapeseed oil (RO) contains a large amount of oleic acid and has significant contents of linoleic and linolenic acids, i.e. a high global content of unsaturation-rich residues. Thus, EIE of BF with RO may bring nutritional improvements to the reaction product, when compared to BF alone. The EIE of BF and canola oil (a low-erucic acid RO) catalyzed by the immobilized sn-1,3 specific Rhizopus arrhizus lipase in solvent-free batch and micro-aqueous systems, was previously studied. The aim of the present study was first to assess the evolution of chemical, physical and thermal modifications occurring during solvent-free batch EIE of BF and RO, with the use of lipozyme TL IM. The evolution of TAG profiles, interesterification degree, dropping point, solid fat content and free fatty acids was monitored during the reaction, especially during the first hours. Differential scanning calorimetry was also applied to follow the formed product. Then the establishment of relations between the DP and differential scanning calorimetry data and the interesterification degree was emphasized. [less ▲]

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See detailHuman T-cell leukemia virus type-1 Tax oncoprotein regulates G-protein signaling.
Twizere, Jean-Claude ULg; Springael, Jean-Yves; Boxus, Mathieu ULg et al

in Blood (2007), 109(3), 1051-60

Human T-cell leukemia virus type-1 (HTLV-1) is associated with adult T-cell leukemia (ATL) and neurological syndromes. HTLV-1 encodes the oncoprotein Tax-1, which modulates viral and cellular gene ... [more ▼]

Human T-cell leukemia virus type-1 (HTLV-1) is associated with adult T-cell leukemia (ATL) and neurological syndromes. HTLV-1 encodes the oncoprotein Tax-1, which modulates viral and cellular gene expression leading to T-cell transformation. Guanine nucleotide-binding proteins (G proteins) and G protein-coupled receptors (GPCRs) constitute the largest family of membrane proteins known and are involved in the regulation of most biological functions. Here, we report an interaction between HTLV-1 Tax oncoprotein and the G-protein beta subunit. Interestingly, though the G-protein beta subunit inhibits Tax-mediated viral transcription, Tax-1 perturbs G-protein beta subcellular localization. Functional evidence for these observations was obtained using conditional Tax-1-expressing transformed T-lymphocytes, where Tax expression correlated with activation of the SDF-1/CXCR4 axis. Our data indicated that HTLV-1 developed a strategy based on the activation of the SDF-1/CXCR4 axis in the infected cell; this could have tremendous implications for new therapeutic strategies. [less ▲]

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See detailGenetic variability of lactoferrin content estimated by mid-infrared spectrometry in bovine milk
Soyeurt, Hélène ULg; Colinet, Frédéric ULg; Arnould, Valérie ULg et al

in Journal of Dairy Science (2007), 90(9), 4443-4450

The effects of lactoferrin (LF) on the immune system have already been shown by many studies. Unfortunately, the current methods used to measure LF levels in milk do not permit the study of the genetic ... [more ▼]

The effects of lactoferrin (LF) on the immune system have already been shown by many studies. Unfortunately, the current methods used to measure LF levels in milk do not permit the study of the genetic variability of lactoferrin or the performance of routine genetic evaluations. The first aim of this research was to derive a calibration equation permitting the prediction of LF in milk by mid-infrared spectrometry (MIR). The calibration with partial least squares on 69 samples showed a ratio of standard error of cross-validation to standard deviation equal to 1.98. Based on this value, the calibration equation was used to establish an LF indicator trait (predicted LF; pLF) on a large number of milk samples (n = 7,690). A subsequent study of its variability was conducted, which confirmed that stage of lactation and lactation number influence the overall pLF level. Small differences in mean pLF among 7 dairy breeds were also observed. The pLF content of Jersey milk was significantly higher than that in Holstein milk. Therefore, the choice of breed could change the expected LF level. Heritability estimated for pLF was 19.7%. The genetic and phenotypic correlations between somatic cell score and pLF were 0.04 and 0.26, respectively. As somatic cell score increases in presence of mastitis, this observation seems to indicate that pLF, or a function of observed pLF, compared with expected LF might have potential as an indicator of mastitis. The negative genetic correlation (−0.36) between milk yield and pLF could indicate an undesirable effect of selection for high milk production on the overall LF level. [less ▲]

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See detailEffects Of Oral Supplementation With Bovine Colostrum On The Immune System Of Weaned Piglets
Boudry, Christelle ULg; Buldgen, André; Portetelle, Daniel ULg et al

in Research In Veterinary Science (2007), 83(1), 91-101

The aim of this study was to evaluate the influence of bovine colostrum supplementation on the immune system of weaned piglets in a context of a full ban on in-feed antibiotics. After weaning at 21 days ... [more ▼]

The aim of this study was to evaluate the influence of bovine colostrum supplementation on the immune system of weaned piglets in a context of a full ban on in-feed antibiotics. After weaning at 21 days, 24 outbred piglets were fed with a diet supplemented daily for three weeks with 0, 1 or 5 g of colostrum. Feed intake, growth performance, haematological parameters, and serum and local anti-colostrum immunoglobulin levels were examined. Lymphocytes from the blood, spleen and gut-associated lymphoid tissues were analysed for phenotype as well as for their ability to produce cytokines. The stimulation index (SI) of mononuclear cells from different organs was obtained after colostral or mitogenic stimulation. Feed intake, growth and haematological parameters were not significantly affected by colostrum. Total serum IgA levels were increased after colostrum supplementation, with a transient decrease in total IgG. Local anti-colostrum immunization was observed in colostrum-fed piglets. The CD21+/CD3+ cells populations of the ileal Peyer’s patch (iPP) were markedly affected. The SI of lymphocyte populations changed significantly whereas, naive blood lymphocytes were not stimulated in vitro in the presence of bovine colostrum, suggesting local anti-colostrum immunization and an absence of direct mitogenic effects of the colostrum. Both Th1 and Th2 cytokine production was present in the different organs of colostrum-fed piglets. Bovine colostrum especially stimulated iPP cells. [less ▲]

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See detailMapping of the bovine growth hormone secretagogue receptor (GHS-R) and polymorphism study in cattle
Colinet, Frédéric ULg; Eggen, André; Gengler, Nicolas ULg et al

Poster (2006, December)

A third control pathway of the Growth Hormone (GH) secretion has come into picture since the development of synthetic compounds known as Growth Hormone Secretagogues (GHSs). The GHS Receptor (GHS-R) and ... [more ▼]

A third control pathway of the Growth Hormone (GH) secretion has come into picture since the development of synthetic compounds known as Growth Hormone Secretagogues (GHSs). The GHS Receptor (GHS-R) and its subtype are abundantly located in the hypothalamus-pituitary unit, but are also distributed in other central areas and peripheral tissues. The GHS-R belongs to the G-protein coupled receptor family with seven transmembrane domain architecture. In order to determine the GHS-R gene sequence, total mRNA was extracted from abomasum and two types of GHS-R cDNA were identified. These two types are transcript variants (1a and 1b) of the same GHS-R gene. The gene encompasses two exons and a single intron. Using a 3000 Rad hybrid panel, the GHS-R gene was mapped to Bos taurus autosome 1 (BTA 1). This localization on BTA 1 agrees totally with comparative data between cattle and human since BTA 1 corresponds to part of human chromosome 3 where human GHSR is also mapped. By two-point analysis, most significantly linked marker are BL26 and BMS4031 (both LOD score : 5,66). Some studies detected different QTLs near these markers like for growth rate, carcass yield, milk portein and milk yield. In the cattle industry, it is of economical importance to increase plasma GH secretion because it is associated with faster growth, less fat stores and improved milk production. Being of economical importance and the detected QTLs near the GHS-R gene, it would be interesting to study the polymorphism on the bovine GHS-R gene. Screening for polymorphisms in the two exons on ten Belgian Blue bulls, ten Holsteins bulls and ten Limousin bulls revealed a total of four single nucleotide polymorphisms (SNPs): three SNPs are in the first exon and one SNP in the second exon. In order to evaluate if GHS-R could be involved in genetic variation for growth rate, carcass yield, milk portein and milk yield, an association study between SNPs on GHS-R gene and these traits could be performed in a major cattle population. [less ▲]

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See detailIsolation of insecticidal proteins within the pea seeds (Pisum sativum L.)
Cuartero Diaz, Gaëtan; Francis, Frédéric ULg; Portetelle, Daniel ULg et al

Poster (2006, October)

Consequently with the pressure exerted by chemical pesticides on environment, and the awakening of politics, the demand for bio-pesticides is increasing. Nevertheless, supply is not sufficient, and ... [more ▼]

Consequently with the pressure exerted by chemical pesticides on environment, and the awakening of politics, the demand for bio-pesticides is increasing. Nevertheless, supply is not sufficient, and moreover those products are not competing enough. In this context, the aim of this research is to set up a biological insecticide, which is economic, with vegetal proteins resulting from alimentary industry, here the pea, Pisum sativum L.. A group of proteins, which is quite easy to highlight, is present in relatively important proportions (2%) in pea seeds, it’s the lectins class. Insecticidal effects of lectins from different organisms have already been proved. Indeed, by binding to membrane glycosyl groups of digestive tract cells, lectins can be very toxic for a lot of insects. Thus initially we focus our investigations on Pisum sativum lectins (PSL). First, PSL have been localised within the industrial process among different extraction juices. Then, a chromatography has been performed on the selected juice with FPLC technology. Although the matrix used for this chromatography, sephadex G75, is a banal bed for gel filtration, it is in this case a real combination between classical gel filtration and affinity chromatography. Indeed due the particular properties of lectins, they fixed carbonyl group of the bed and have to be eluted after the filtration part with a solution of glucose. Then the collected fractions corresponding to UV-peaks on the chromatogram were separated by electrophoresis 2D and identified by mass spectrometry (ESI MS/MS) coupled with data bank investigations. Secondly bioassays using artificial diets have been developed on Myzus persicae in the aim to study the aphicid effects of theses fractions with rm and LC50.These estimators show significant mortality rates but also change in the fecundity and in the development of nymph. [less ▲]

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See detailValorisation du colostrum bovin. Rapport Scientifique et Technique Final
Halleux, Caroline; Boudry, Christelle ULg; Portetelle, Daniel ULg et al

Report (2006)

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See detailMapping and polymorphism of bovine ghreline gene
Colinet, Frédéric ULg; Eggen, André; Gengler, Nicolas ULg et al

in Proceedings of the 30th International Conference on Animal Genetics ISAG 2006 - Biodiversity, the future pass through preservation (2006, August)

Bovine ghrelin, a 27-amino-acid peptide has been identified in bovine oxyntic glands of the abomasum. It is an endogenous growth hormone secretagogue. Total mRNA was extracted from abomasum and complete ... [more ▼]

Bovine ghrelin, a 27-amino-acid peptide has been identified in bovine oxyntic glands of the abomasum. It is an endogenous growth hormone secretagogue. Total mRNA was extracted from abomasum and complete ghrelin mRNA was sequenced by rapid amplification of cDNA ends. The gene contains five exons and four introns with a short noncoding first exon of 17 bp similar to mouse and human ghrelin gene. Using a radiation hybrid panel, the gene was mapped to chromosome 22 near microsatellite markers UWCA49, BM4102, BMS1932, BM2613 and URB035 with good LOD Score. Some studies detected different QTLs near these markers like for milk fat percent, milk protein percent and somatic cell score. So, it would be interesting to study the polymorphism on the bovine ghrelin gene. Screening for polymorphisms in the five exons and the introns II and IV on ten Belgian Blue bulls, ten Holsteins bulls and ten Limousin bulls revealed a total of three single nucleotide polymorphisms. In order to evaluate if ghrelin could be involved in genetic variation for milk fat percent, milk protein percent and somatic cell score an association study between SNPs on ghrelin gene and these traits could be performed in a major cattle population. [less ▲]

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See detailMapping and polymorphism of bovine ghreline gene
Colinet, Frédéric ULg; Eggen, André; Halleux, Caroline et al

Poster (2006, August)

Bovine ghrelin, a 27-amino-acid peptide has been identified in bovine oxyntic glands of the abomasum. It is an endogenous growth hormone secretagogue. Total mRNA was extracted from abomasum and complete ... [more ▼]

Bovine ghrelin, a 27-amino-acid peptide has been identified in bovine oxyntic glands of the abomasum. It is an endogenous growth hormone secretagogue. Total mRNA was extracted from abomasum and complete ghrelin mRNA was sequenced by rapid amplification of cDNA ends. The gene contains five exons and four introns with a short noncoding first exon of 17 bp similar to mouse and human ghrelin gene. Using a radiation hybrid panel, the gene was mapped to chromosome 22 near microsatellite markers UWCA49, BM4102, BMS1932, BM2613 and URB035 with good LOD Score. Some studies detected different QTLs near these markers like for milk fat percent, milk protein percent and somatic cell score. So, it would be interesting to study the polymorphism on the bovine ghrelin gene. Screening for polymorphisms in the five exons and the introns II and IV on ten Belgian Blue bulls, ten Holsteins bulls and ten Limousin bulls revealed a total of three single nucleotide polymorphisms. In order to evaluate if ghrelin could be involved in genetic variation for milk fat percent, milk protein percent and somatic cell score an association study between SNPs on ghrelin gene and these traits could be performed in a major cattle population. [less ▲]

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See detailProduction and characterization of monoclonal and polyclonal antibodies against Scd14
Halleux, Caroline; Boudry, Christelle ULg; Thewis, André ULg et al

Poster (2006, May 17)

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See detailEffect Of Bovine Colostrum Supplementation On Cytokine mRNA Expression In Weaned Piglets
Boudry, Christelle ULg; Buldgen, André; Portetelle, Daniel ULg et al

in 10th International symposium on digestive physiology in pigs (2006, May)

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See detailValorisation du colostrum bovin. Rapport Scientifique et technique. Année 3.
Halleux, Caroline; Boudry, Christelle ULg; Portetelle, Daniel ULg et al

Report (2006)

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See detailQuantification of Bifidobacterium spp. and Lactobacillus spp. in rat fecal samples by real-time PCR.
Delroisse, Jean-Marc; Boulvin, Anne-Lise; Parmentier, Isabelle et al

in Microbiological research (2006), (5),

Detailed reference viewed: 57 (4 ULg)