References of "Portetelle, Daniel"
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See detailEffect Of A Bovine Colostrum Whey Supplementation On Growth Performance, Faecal Escherichia Coli Population And Systemic Immune Response Of Piglets At Weaning
Boudry, Christelle ULg; Dehoux, Jean-Paul; Wavreille, José et al

in Animal (2008), 2(5), 730-737

This study examined the effect of a bovine colsotrum whey supplementation on growth performance, feed intake, faecal Escherichia coli population and systemic immune response of piglets.

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See detailLes progrès de la microbiologie: de Louis Pasteur à la "microbiomique"
Portetelle, Daniel ULg

Conference given outside the academic context (2008)

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See detailSurvival of yolk’s immunoglobulins directed against Salmonella Enteritidis and Salmonella Typhimurium in the gastro-intestinal tract of the broiler chicken
Marcq, Christopher ULg; Graide, Virginie; Beckers, Yves ULg et al

in Proceedings of the Thirteenth Conference on Food Microbiology (2008)

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See detailBovine colostrum as a natural growth promoter for newly weaned piglets: a review.
Boudry, Christelle ULg; Dehoux, Jean-Paul; Portetelle, Daniel ULg et al

in Biotechnologie, Agronomie, Société et Environnement = Biotechnology, Agronomy, Society and Environment [=BASE] (2008), 12(2), 157-170

The aim of this review is to present the potential of bovine colostrum as growth promoter in piglet-weaner diet. The consequences of weaning on the growth performance, on the gastro-intestinal tract and ... [more ▼]

The aim of this review is to present the potential of bovine colostrum as growth promoter in piglet-weaner diet. The consequences of weaning on the growth performance, on the gastro-intestinal tract and on the metabolic and endocrine systems of the piglet are described in the first part of this review. The second part is dedicated to the bovine colostrum, with a description of the actions due to its main growth promoters and antimicrobial factors. Finally, the reported effects of bovine specific components or bovine fractions on the growth performance and on the structure and function of the gastro-intestinal tract of piglets in the early postweaning period are presented. They show clearly the potential of bovine colostrum to reduce the growth-check related to the weaning of the piglet. [less ▲]

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See detailImmunomodulatory Properties Of Two Wheat Bran Fractions - Aleurone-Enriched And Crude Fractions - In Obese Mice Fed A High Fat Diet
Neyrinck, Am.; De Backer, F.; Cani, Pd. et al

in International Immunopharmacology (2008), 8(10),

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See detailProduction Of Hen Egg Yolk Immunoglobulins Simultaneously Directed Against Salmonella Enteritidis And Salmonella Typhimurium In The Same Egg Yolk
Chalghoumi, Raja ULg; Thewis, André ULg; Portetelle, Daniel ULg et al

in Poultry Science (2008), 87(1), 32-40

The present study was an attempt to raise hen egg yolk Ig (IgY) simultaneously directed against Salmonella Enteritidis (SE) and Salmonella Typhimurium (ST) in the same egg yolk. The immunopotentiating ... [more ▼]

The present study was an attempt to raise hen egg yolk Ig (IgY) simultaneously directed against Salmonella Enteritidis (SE) and Salmonella Typhimurium (ST) in the same egg yolk. The immunopotentiating effect of 2 different adjuvants—Freund’s adjuvants (FA) and immunostimulating complexes matrix (IM)—on antibody response was also evaluated. Bacterial outer membrane proteins (OMP) were selected as target antigens. The ISA Brown hens, specific-Salmonella spp.-free status, divided into 6 groups were intramuscularly injected with a monocompound antigen preparation: SE-OMP (treatment SEFA or SE-IM) or ST-OMP (treatment ST-FA or ST-IM), or a combined antigen preparation: ¹⁄₂ SE-OMP and ¹⁄₂ STOMP (treatment SEST-FA or SEST-IM). Titers of antibodies in yolk were evaluated biweekly with ELISA. There was no antigen × adjuvant interaction on antibody titers. Anti-SE IgY titers in hens that received treatment SESTFA or SEST-IM were statistically similar (P > 0.05) as compared with those obtained from hens immunized with treatment SE-FA or SE-IM. Anti-ST IgY titers in hens immunized with treatment SEST-FA or SEST-IM were slightly lower than those of hens that received treatment ST-FA or ST-IM. The cross-reactivity of anti-SE IgY, induced by treatment SE-FA or SE-IM, with ST-OMP antigen and that of anti-ST IgY, induced by ST-FA or ST-IM, with SE-OMP antigen were arbitrarily assessed on d 43 and 155 by ELISA. The average cross-reactivity of anti-SE IgY with ST-OMP antigen was 71.7%. The average cross-reactivity of anti-ST IgY with SE-OMP antigen was 78.8%. In FA groups, antibody titers were found higher (P < 0.05) than those in IM groups. Furthermore, no extensive lesions or clinical abnormalities were detected in hens injected with FA. These findings showed the opportunity to raise IgY antibody against 2 Salmonella serovars in the same yolk and that FA was more efficient than IM in mediating antibody response. [less ▲]

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See detailEffect Of Bovine Colostrum Supplementation On Cytokine mRNA Expression In Weaned Piglets
Boudry, Christelle ULg; Buldgen, André; Portetelle, Daniel ULg et al

in Livestock Science (2007), 108(1-3), 295-298

The aim of this study was to evaluate the influence of a bovine colostrum supplementation on the immune Th1/Th2 response in weaned piglets. After weaning at 21 days, 21 piglets were fed daily ad libitum ... [more ▼]

The aim of this study was to evaluate the influence of a bovine colostrum supplementation on the immune Th1/Th2 response in weaned piglets. After weaning at 21 days, 21 piglets were fed daily ad libitum with a starter diet supplemented for 3 weeks with 0, 1 or 5 g of bovine colostrum. Growth performances were measured weekly. Spleen and gut-associated lymphoid tissues (GALT) (ileal Peyer’s patch (iPP), jejunum wall (JW) and mesenteric lymph nodes (MLN)) biopsies were collected on each piglet after the 3 weeks treatment and analyzed for their cytokine mRNA expression (IL-2, IL-4, IL-10, IL-12 and IFN) by RT-PCR. Growth performances were not affected by bovine colostrum (P > 0.05). In the colostrum groups, the iPP cells showed higher IL-4, IL-10 and IL-12 cytokine levels (P < 0,05), associated with a decrease of IFN-γ (P < 0,01) and no effect on IL-2 production (P > 0,05). IL-10, IL-2 and IL-12 productions increased (P < 0,05) in MLN, with no effect on IFN-γ and IL-4 levels (P > 0,05). The IL-12 production was also increased (P < 0,01) in JW cells with no modification for the other cytokine. No modification of cytokine production was observed in the spleen (P > 0,05). These results suggest an immunomodulatory effect of bovine colostrum on the GALT which answered by producing, at different levels, both Th1 pro-inflammatory cytokines - IL-2, IFN-γ and IL-12 - and Th2 anti-inflammatory cytokines - IL-4 and IL-10. This bipolarity of the Th1/Th2 response protects the weaned piglets from both allergic (food) as well as infectious (pathogens) diseases. [less ▲]

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See detailLipase-catalyzed interesterification of butterfat with rapeseed oil: new approaches for the monitoring of the reaction.
Hanon, Emilien ULg; Aguedo, Mario ULg; Danthine, Sabine ULg et al

Poster (2007)

Butterfat (BF) is one main source of diet fats. However, it has been less and less well perceived due to its poor spreadability when refrigerated and cholesterol and saturated fatty acids, promoters of ... [more ▼]

Butterfat (BF) is one main source of diet fats. However, it has been less and less well perceived due to its poor spreadability when refrigerated and cholesterol and saturated fatty acids, promoters of coronary heart diseases. Thus, consumer’s demand for healthy palatable fat spreads with good development of modified butter-based spreads. One ordinary method used by manufacturers for such modifications is enzymatic interesterification of a lipase to restructure triacylglycerides (TAG), i.e. to induce the exchange of fatty acid residues amongst glycerol backbones. This leads to changes in TAG species and in physical properties of the fat, namely in solid fat content (SFC) and in melting profile. Rapeseed oil (RO) contains a large amount of oleic acid and has significant contents of linoleic and linolenic acids, i.e. a high global content of unsaturation-rich residues. Thus, EIE of BF with RO may bring nutritional improvements to the reaction product, when compared to BF alone. The EIE of BF and canola oil (a low-erucic acid RO) catalyzed by the immobilized sn-1,3 specific Rhizopus arrhizus lipase in solvent-free batch and micro-aqueous systems, was previously studied. The aim of the present study was first to assess the evolution of chemical, physical and thermal modifications occurring during solvent-free batch EIE of BF and RO, with the use of lipozyme TL IM. The evolution of TAG profiles, interesterification degree, dropping point, solid fat content and free fatty acids was monitored during the reaction, especially during the first hours. Differential scanning calorimetry was also applied to follow the formed product. Then the establishment of relations between the DP and differential scanning calorimetry data and the interesterification degree was emphasized. [less ▲]

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See detailHuman T-cell leukemia virus type-1 Tax oncoprotein regulates G-protein signaling.
Twizere, Jean-Claude ULg; Springael, Jean-Yves; Boxus, Mathieu ULg et al

in Blood (2007), 109(3), 1051-60

Human T-cell leukemia virus type-1 (HTLV-1) is associated with adult T-cell leukemia (ATL) and neurological syndromes. HTLV-1 encodes the oncoprotein Tax-1, which modulates viral and cellular gene ... [more ▼]

Human T-cell leukemia virus type-1 (HTLV-1) is associated with adult T-cell leukemia (ATL) and neurological syndromes. HTLV-1 encodes the oncoprotein Tax-1, which modulates viral and cellular gene expression leading to T-cell transformation. Guanine nucleotide-binding proteins (G proteins) and G protein-coupled receptors (GPCRs) constitute the largest family of membrane proteins known and are involved in the regulation of most biological functions. Here, we report an interaction between HTLV-1 Tax oncoprotein and the G-protein beta subunit. Interestingly, though the G-protein beta subunit inhibits Tax-mediated viral transcription, Tax-1 perturbs G-protein beta subcellular localization. Functional evidence for these observations was obtained using conditional Tax-1-expressing transformed T-lymphocytes, where Tax expression correlated with activation of the SDF-1/CXCR4 axis. Our data indicated that HTLV-1 developed a strategy based on the activation of the SDF-1/CXCR4 axis in the infected cell; this could have tremendous implications for new therapeutic strategies. [less ▲]

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See detailGenetic variability of lactoferrin content estimated by mid-infrared spectrometry in bovine milk
Soyeurt, Hélène ULg; Colinet, Frédéric ULg; Arnould, Valérie ULg et al

in Journal of Dairy Science (2007), 90(9), 4443-4450

The effects of lactoferrin (LF) on the immune system have already been shown by many studies. Unfortunately, the current methods used to measure LF levels in milk do not permit the study of the genetic ... [more ▼]

The effects of lactoferrin (LF) on the immune system have already been shown by many studies. Unfortunately, the current methods used to measure LF levels in milk do not permit the study of the genetic variability of lactoferrin or the performance of routine genetic evaluations. The first aim of this research was to derive a calibration equation permitting the prediction of LF in milk by mid-infrared spectrometry (MIR). The calibration with partial least squares on 69 samples showed a ratio of standard error of cross-validation to standard deviation equal to 1.98. Based on this value, the calibration equation was used to establish an LF indicator trait (predicted LF; pLF) on a large number of milk samples (n = 7,690). A subsequent study of its variability was conducted, which confirmed that stage of lactation and lactation number influence the overall pLF level. Small differences in mean pLF among 7 dairy breeds were also observed. The pLF content of Jersey milk was significantly higher than that in Holstein milk. Therefore, the choice of breed could change the expected LF level. Heritability estimated for pLF was 19.7%. The genetic and phenotypic correlations between somatic cell score and pLF were 0.04 and 0.26, respectively. As somatic cell score increases in presence of mastitis, this observation seems to indicate that pLF, or a function of observed pLF, compared with expected LF might have potential as an indicator of mastitis. The negative genetic correlation (−0.36) between milk yield and pLF could indicate an undesirable effect of selection for high milk production on the overall LF level. [less ▲]

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See detailEffects Of Oral Supplementation With Bovine Colostrum On The Immune System Of Weaned Piglets
Boudry, Christelle ULg; Buldgen, André; Portetelle, Daniel ULg et al

in Research In Veterinary Science (2007), 83(1), 91-101

The aim of this study was to evaluate the influence of bovine colostrum supplementation on the immune system of weaned piglets in a context of a full ban on in-feed antibiotics. After weaning at 21 days ... [more ▼]

The aim of this study was to evaluate the influence of bovine colostrum supplementation on the immune system of weaned piglets in a context of a full ban on in-feed antibiotics. After weaning at 21 days, 24 outbred piglets were fed with a diet supplemented daily for three weeks with 0, 1 or 5 g of colostrum. Feed intake, growth performance, haematological parameters, and serum and local anti-colostrum immunoglobulin levels were examined. Lymphocytes from the blood, spleen and gut-associated lymphoid tissues were analysed for phenotype as well as for their ability to produce cytokines. The stimulation index (SI) of mononuclear cells from different organs was obtained after colostral or mitogenic stimulation. Feed intake, growth and haematological parameters were not significantly affected by colostrum. Total serum IgA levels were increased after colostrum supplementation, with a transient decrease in total IgG. Local anti-colostrum immunization was observed in colostrum-fed piglets. The CD21+/CD3+ cells populations of the ileal Peyer’s patch (iPP) were markedly affected. The SI of lymphocyte populations changed significantly whereas, naive blood lymphocytes were not stimulated in vitro in the presence of bovine colostrum, suggesting local anti-colostrum immunization and an absence of direct mitogenic effects of the colostrum. Both Th1 and Th2 cytokine production was present in the different organs of colostrum-fed piglets. Bovine colostrum especially stimulated iPP cells. [less ▲]

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