References of "Ponthier, Jérôme"
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See detailMyeloperoxidase in Equine Semen: Concentration and Localization during Freezing Processing
Ponthier, Jérôme ULg; Desvals, Maud; Franck, Thierry ULg et al

in Journal of Equine Veterinary Science (2012), (32), 32-37

Myeloperoxidase (MPO) is a pro-oxidant enzyme contained in and released by neutro- phils during degranulation or after lysis. Post-thaw semen contains MPO, and concen- tration of this enzyme is associated ... [more ▼]

Myeloperoxidase (MPO) is a pro-oxidant enzyme contained in and released by neutro- phils during degranulation or after lysis. Post-thaw semen contains MPO, and concen- tration of this enzyme is associated with decreased motility. The aim of this study was to determine kinetics of MPO concentration during freezing, its origin, and its impact on frozenethawed semen. Forty ejaculates were used. Semen was frozen using the classical freezing procedure. MPO concentrations were assayed in fresh semen, after centrifuga- tion, and after cooling down to 4 C. Post-thaw MPO assay results and spermogram characteristics were determined. MPO immunocytochemistry was performed on 4 different ejaculates at each step of freezing procedure. MPO concentration increased after cooling down to 4 C and thawing compared with fresh semen. As temperature decreased, MPO was higher or tended to be higher in post-thaw poor quality samples. Nonsperm cells showed various degrees of MPO immunostaining and were observed as epithelial cells with nuclear pyknosis and keratinization. MPO immunostaining increased in medium and decreased in nonsperm cells during freezing. Our study shows that MPO concentration in equine semen increases when temperature decreases. We hypothesize that nonsperm cells present in fresh semen could release MPO. [less ▲]

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See detailSperm quality analysis in XX, XY and YY males of the Nile tilapia (Oreochromis niloticus).
Gennotte, Vincent ULg; François, Ekniel; Rougeot, Carole ULg et al

in Theriogenology (2012)

In Nile tilapia (Oreochromis niloticus), individuals with atypical sexual genotype are commonly used in farming (use of YY males to produce all-male offsprings), but they also constitute major tools to ... [more ▼]

In Nile tilapia (Oreochromis niloticus), individuals with atypical sexual genotype are commonly used in farming (use of YY males to produce all-male offsprings), but they also constitute major tools to study sex determinism mechanisms. In other species, sexual genotype and sex reversal procedures affect different aspects of biology such as growth, behaviour and reproductive success. The aim of this study was to assess the influence of sexual genotype on sperm quality in Nile tilapia. Milt characteristics were compared in XX (sex-reversed), XY and YY males in terms of gonadosomatic index, sperm count, sperm motility and duration of sperm motility. Sperm motility was measured by computer-assisted sperm analysis (CASA) quantifying several parameters: total motility, progressive motility, curvilinear velocity, straight line velocity, average path velocity and linearity. None of the sperm trait measured differed significantly between the three genotypes. Mean values of gonadosomatic index, sperm concentration and sperm motility duration of XX, XY and YY males respectively ranged from 0.92 to 1.33 %, from 1.69 to 2.22 × 10(9) cells mL-1 and from 18’04’’ to 27’32’’. Mean values of total motility and curvilinear velocity 1 min after sperm activation respectively ranged from 53 to 58 % and from 71 to 76 µm s-1 for the three genotypes. After 3 min of activity, all the sperm motility and velocity parameters dropped by half and continued to slowly decrease thereafter. Seven min after activation, only 9 to 13 % of spermatozoa were still progressive. Our results prove that neither sexual genotype nor hormonal sex reversal treatments affect sperm quality in male Nile tilapias with atypical sexual genotype. [less ▲]

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See detailDystocies équines: Données descriptives et approche de la gestion des soins intensifs
Deleuze, Stefan ULg; de la Rebière de Pouyade, Geoffroy ULg; Sandersen, Charlotte ULg et al

Conference (2011, December 02)

La fréquence de fourbures rencontrées après une dystocie semble faible par rapport à la littérature. L’ajout d’héparines fractionnées aux traitements habituels de la métrite du post-partum (ocytocine ... [more ▼]

La fréquence de fourbures rencontrées après une dystocie semble faible par rapport à la littérature. L’ajout d’héparines fractionnées aux traitements habituels de la métrite du post-partum (ocytocine, lavage utérins, antibiotiques et anti-inflammatoires) semble intéressant pour réduire le risque de fourbure. [less ▲]

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See detailGestion du poulinage
Ponthier, Jérôme ULg; Deleuze, Stefan ULg

Conference given outside the academic context (2011)

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See detailEQUINE SEMEN FREEZING: STATE OF THE ART AND PERSPECTIVES.
Ponthier, Jérôme ULg

Conference (2011, November 19)

Depuis vingt ans, la congélation du sperme a permis de diffuser la génétique des étalons. Les congélations effectuées en dehors des saisons sportives permettent d’inséminer de nombreuses juments pendant ... [more ▼]

Depuis vingt ans, la congélation du sperme a permis de diffuser la génétique des étalons. Les congélations effectuées en dehors des saisons sportives permettent d’inséminer de nombreuses juments pendant que les étalons concourent dans des lieux éloignés. Les éleveurs ont en permanence accès à des doses d’insémination sans risques sanitaires. Cependant, malgré une bonne qualité de sperme frais, 20% des éjaculats équins ne supportent pas la congélation (Vidament, 2005). Le but de cette présentation est de revoir la physiologie du spermatozoïde et d’en discuter les implications dans le contexte de la congélation. Les améliorations des techniques de congélation présentes ou à venir seront aussi évoquées. [less ▲]

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See detailMrozenie nasienia u koni - stan obecny i perspektywy
Ponthier, Jérôme ULg

in Gajewski, Zdsislaw; Malinowski, Edward; Wehrend, Axel (Eds.) Rozrod Koni: aktualne poglady (2011, November 19)

Aim of this paper is to review equine semen freezing procedures. Spermatozoa formation inside the testis, spermatozoa anatomy and physiology will be summarized, highlighting structures and/or functions ... [more ▼]

Aim of this paper is to review equine semen freezing procedures. Spermatozoa formation inside the testis, spermatozoa anatomy and physiology will be summarized, highlighting structures and/or functions injured during freezing/thawing process. Current quality standards will be defined for fresh and thawed semen with a special attention on total and progressive motilities and different definitions of these parameters in literature. New ways of semen analysis (as flow cytometry) will be discussed for their scientific and clinical implications. Freezing procedures will and new freezing procedures will be discussed. Future developments and progress in freezing methods will be exposed, as prediction of post-thaw semen quality on fresh semen basis, improvement of freezing extenders and prevention of reactive oxygen species effects. [less ▲]

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See detailEffect of Deferoxamine Mesylate on Freezability of Blood Supplemented Canine Semen
Van Den Berghe, Femke ULg; Rijsselaere, Tom; Van Soom, Ann et al

Poster (2011, September)

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See detailSuperovulation in the mare with commercially available pFSH
Parilla Hernandez, Sonia; Deleuze, Stefan ULg; Beckers, Jean-François ULg et al

in Reproduction in Domestic Animals (2011), 46(s3), 138

Further studies on Stimufol with larger numbers, should be conducted as it might prove inexpensive and easy to superovulate mares.

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See detailGestion de la jument poulinière et de l'étalon
Ponthier, Jérôme ULg; Deleuze, Stefan ULg

Conference given outside the academic context (2011)

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See detailLa spermatogenèse, la libido et la dose
Ponthier, Jérôme ULg; Lecrenier, Marie-Caroline; Parrilla Hernandez, Sonia ULg et al

Conference given outside the academic context (2010)

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See detailAssociation between Myeloperoxidase concentration in equine frozen semen and post thawing parameters
Ponthier, Jérôme ULg; Franck, Thierry ULg; Detilleux, Johann ULg et al

in Reproduction in Domestic Animals (2010), 45(5), 811-816

Despite improvement of techniques, semen of 20% of stallions remains unfreezable. Recent studies focused on impact of reactive oxygen species and oxidant enzymes on semen characteristics. Myeloperoxidase ... [more ▼]

Despite improvement of techniques, semen of 20% of stallions remains unfreezable. Recent studies focused on impact of reactive oxygen species and oxidant enzymes on semen characteristics. Myeloperoxidase (MPO) is a pro-oxidant enzyme contained in and released by neutrophils during degranulation or after cell lysis. It is responsible for the formation of hypochlorous acid, a strong oxidant agent which could damage spermatozoa. The aim of this study was to determine the relation between MPO concentration and characteristics of frozen semen from stallions. Thirty five straws from different stallions were analyzed. Post-thawing spermatozoal concentration, progressive and total motility were determined by CASA. Freezability was determined according to post-thawing progressive motility (over or under 15%). Percent of alive spermatozoa and abnormal forms were determined after Eosin-Nigrosin and Diff-Quick® staining respectively. Post-thawing MPO concentration was measured by ELISA. Our study shows that frozen thawed semen contains large amounts of free MPO. We also observed that post-thawing MPO ELISA assay can be used as an indicator of equine semen freezability. High MPO concentration samples showed lower total and progressive motility. A higher proportion of abnormal head shape associated with acrosome reaction was observed in our late examinations of the high concentration MPO group. Our results show that MPO adversely affects total and progressive motility of equine semen. A negative correlation between normal motile forms and MPO concentration was also observed. The effect of MPO on dead or abnormal forms remains to be precised. [less ▲]

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See detailCytological diagnosis of endometritis in the mare: a comparative study
Daspet, Sarah-Morgane; Ponthier, Jérôme ULg; Jolly, Sandra ULg et al

in Reproduction in Domestic Animals (2010, September), 45s3

It was concluded that the brush swab was a promising diagnostic tool for use in field conditions.

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See detailIs neutrophil elastase associated with myeloperoxidase concentration and post-thawing parameters in equine frozen semen?
Ponthier, Jérôme ULg; de la Rebière de Pouyade, Geoffroy ULg; Desvals, Maud et al

in Animal Reproduction Science (2010), 121S

Non sperm cells concentration is higher in unfreezable semen. A relation between non-sperm cells in fresh semen and MPO and post thawing quality has been observed. Neutrophil Elastase seems to have no ... [more ▼]

Non sperm cells concentration is higher in unfreezable semen. A relation between non-sperm cells in fresh semen and MPO and post thawing quality has been observed. Neutrophil Elastase seems to have no effect on semen characteristics and to be not associated with on-sperm cells and freezability. [less ▲]

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See detailMicroscopic study of equine oocyte maturation within quaternary follicles.
Ponthier, Jérôme ULg; Beckers, Jean-François ULg; Deleuze, Stefan ULg

Conference (2009, February 19)

In various species a relationship between oocyte maturity and morphological characteristics of oocytes has been established. To our knowledge, immature oocyte aspect under light microscopy has been paid ... [more ▼]

In various species a relationship between oocyte maturity and morphological characteristics of oocytes has been established. To our knowledge, immature oocyte aspect under light microscopy has been paid little attention in the equine. The aim of the study is to give a general description and analyse microscopic parameters of immature oocytes. Follicles from abattoir ovaries are punctured after measurement of their diameter and their cumulus-oocyte complexes are recovered. Different characters such as oocyte diameter, cumulus aspect, granulosity and polarity of ooplasm are observed under light microscopy. No correlation between estral activity, follicle maturity, cumulus cells aspect, polarity and granulosity of ooplasm was observed. Thickness of zona pellucida differs between oocytes with polar or non polar ooplasm. No correlation was observed between follicle diameter and oocyte diameter and none of the other studied parameters showed an influence on oocyte diameter. Results show that no character of oocytes observed under light microscopy can be related to follicular origin or to any other character. Further studies have to be done to compare these immature oocytes with mature oocytes and to find the ultra-structural origin of characters observed under light microscopy. [less ▲]

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See detailAffections du système reproducteur et équitation - quand les hormones s'en mêlent
Ponthier, Jérôme ULg; Deleuze, Stefan ULg

Conference given outside the academic context (2009)

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See detailMicroscopic study of equine oocyte maturation within quaternary follicles
Ponthier, Jérôme ULg; Beckers, Jean-François ULg; Deleuze, Stefan ULg

in Comptes Rendus de l'Académie Bulgare des Sciences = Proceeding of the Bulgarian Academy of Sciences (2009), 62(4), 491-498

In various species a relationship between oocyte maturity and morphological characteristics of oocytes has been established. To our knowledge, little attention has been paid to the immature oocyte aspect ... [more ▼]

In various species a relationship between oocyte maturity and morphological characteristics of oocytes has been established. To our knowledge, little attention has been paid to the immature oocyte aspect under light microscopy in the equine. The aim of the study is to give a general description and analysis of the microscopic parameters of immature oocytes. Follicles from abattoir ovaries are punctured after measurement of their diameter and their cumulus-oocyte complexes are recovered. Different characters such as oocyte diameter, cumulus aspect, granulosity and polarity of ooplasm are observed under light microscopy. No correlation between the estral activity, follicle maturity, cumulus cells aspect, polarity and granulosity of ooplasm was observed. The thickness of zona pellucida differs between oocytes with polar or nonpolar ooplasm. No correlation was observed between the follicle and oocyte diameter and none of the other studied parameters showed an influence on the oocyte diameter. Results show that no character of the observed oocytes under light microscopy can be related to the follicular origin or to any other character. Further studies have to be done to compare these immature oocytes with mature ones and to find the ultrastructural origin of the characters observed under light microscopy. [less ▲]

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See detailReproduction assistée dans l’espèce équine : collecte, évaluation, maturation et utilisations d’ovocytes équins
Deleuze, Stefan ULg; Ponthier, Jérôme ULg; Hanzen, Christian ULg

in Annales de Médecine Vétérinaire (2009), 153(1), 22-30

Malgré le faible nombre d’ovocytes équins disponibles pour la recherche, des techniques de reproduction assistée récemment développées dans cette espèce permettent maintenant de produire des embryons en ... [more ▼]

Malgré le faible nombre d’ovocytes équins disponibles pour la recherche, des techniques de reproduction assistée récemment développées dans cette espèce permettent maintenant de produire des embryons en utilisant des étalons et des juments subfertiles ou même de sauvegarder leur potentiel génétique après leur mort. Cette revue de littérature décrit les aspects cliniques de la collecte d’ovocytes ex vivo ou in vivo, leur évaluation, leur maturation in vitro et leur utilisation pour le transfert intra-folliculaire ou salpyngien et l’injection intra-cytoplasmique de spermatozoïde. [less ▲]

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See detailEquine frozen semen parameters in relation with total myeloperoxidase concentration
Ponthier, Jérôme ULg; Franck, Thierry ULg; Mottart, Evelyne et al

in Animal Reproduction Science (2008, September 15)

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See detailPregnancy diagnosis in the mare by semi quantitative relaxin quick assay kit
Ponthier, Jérôme ULg; van de Weerdt, marie-Lys; Deleuze, Stefan ULg

in Reproduction in Domestic Animals (2008, July), 43(s3), 111

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See detailLes maladies vénériennes chez le cheval
Ponthier, Jérôme ULg; Deleuze, Stefan ULg

in sBs Magazine (2008)

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