References of "Pincemail, Joël"
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See detailEvidence for free radical generation in ischemia-reperfusion
Franssen, Colette ULg; Pincemail, Joël ULg; Defraigne, Jean-Olivier ULg et al

in Teaching and research in intensive care medicine - Proceeding book (1992)

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See detailSelf-Labeling of Human Polymorphonuclear Leucocyte Myeloperoxidase with 125iodine
Deby, Ginette ULg; Pincemail, Joël ULg; Thirion, A. et al

in Experientia (1991), 47(9), 952-7

In order to obtain a radioimmunoassay (RIA) technique for the measurement of human plasma myeloperoxidase (MPO), we purified the enzyme from polymorphonuclear granulocytes (neutrophils), and compared ... [more ▼]

In order to obtain a radioimmunoassay (RIA) technique for the measurement of human plasma myeloperoxidase (MPO), we purified the enzyme from polymorphonuclear granulocytes (neutrophils), and compared three methods of labeling it with 125Iodine:chloramine T, lactoperoxidase, and an original technique of 'self labeling' based on the ability of the enzyme to oxidize and bind 125I in the presence of H2O2. The chloramine T technique produced a degraded protein, as well shown by a high non-specific binding of tracer to antibody. The lactoperoxidase technique did not succeed in labeling MPO with an adequate specific activity. In contrast, the self-labeling method gave a stable tracer with a specific activity of 23 microCi/micrograms MPO (85 MBq), a satisfactory level of immunoreactivity, and a low-specific binding (less than or equal to 3%). After labeling, purification of tracer was achieved by gel filtration chromatography in phosphate buffer (0.05 M; pH7) to which 0.1% poly-L-lysine was added. The labeled molecule remained stable for 40 days and could be used for RIA with a polyclonal antibody raised in rabbits. [less ▲]

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See detailPlasma Vitamin E, Total Lipids and Myeloperoxidase Levels During Spinal Surgery. A Comparison between Two Anesthetic Agents: Propofol and Isoflurane
Hans, Pol ULg; Canivet, Jean-Luc ULg; Pincemail, Joël ULg et al

in Acta Anaesthesiologica Scandinavica (1991), 35(4), 302-5

Plasma levels of vitamin E (Vit. E), total lipids (TL), Vit. E to TL ratio and myeloperoxidase (MPO) were studied in 20 patients undergoing lumbar spinal surgery and randomly allocated to two anesthetic ... [more ▼]

Plasma levels of vitamin E (Vit. E), total lipids (TL), Vit. E to TL ratio and myeloperoxidase (MPO) were studied in 20 patients undergoing lumbar spinal surgery and randomly allocated to two anesthetic groups: propofol (bolus dose + continuous infusion and thiopental/isoflurane. Peripheral blood samples were withdrawn prior to induction, each hour during anesthesia and 1 h after the end of surgery. Mean Vit. E and TL levels as well as mean Vit. E to TL ratios remained in the normal range over the entire period of study whatever the anesthetic regimen. MPO levels rose significantly in the post-operative period only, but without statistical difference between the two groups. Therefore, anesthesia with propofol or thiopental/isoflurane modifies neither total lipid concentrations nor plasma Vit. E, which is a potent endogenous inhibitor of lipid peroxidation bound to lipoproteins. The rise of plasma MPO suggests a moderate post-operative neutrophil activation which is not influenced by anesthetic techniques. [less ▲]

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See detailFast double antibody radioimmunoassay of human granulocyte myeloperoxidase and its application to plasma.
Pincemail, Joël ULg; Deby-Dupont, G.; Deby, Christiane ULg et al

in Journal of Immunological Methods (1991), 137(2), 181-191

The haem enzyme myeloperoxidase (MPO) (EC 1.11.1.7) with a spectral A430/A280 ratio greater than 0.7 and a specific activity of 125 U/mg was purified from isolated human neutrophils. To obtain a ... [more ▼]

The haem enzyme myeloperoxidase (MPO) (EC 1.11.1.7) with a spectral A430/A280 ratio greater than 0.7 and a specific activity of 125 U/mg was purified from isolated human neutrophils. To obtain a radioimmunoassay (RIA) for this enzyme, a specific antiserum against human neutrophil MPO was raised in rabbits and used at an initial dilution of 1/10,000. MPO labelled with 125iodine by a technique of self-labelling in the presence of H2O2, had a specific activity of 24 mCi/mg. After incubation at room temperature (2 h) and separation by double antibody precipitation in the presence of polyethylene glycol, the sensitivity of the RIA was 21 ng/ml. The RIA showed good precision and accuracy with intra- and interassay coefficients of variation of less than 7% for MPO concentrations ranging from 100 to 800 ng/ml, and satisfactory recoveries of known amounts of exogenous MPO in plasma. For the measurement of MPO in blood, the best sampling technique was to collect blood into EDTA. Rapid centrifugation (within 20 min) was necessary for blood collected into heparin. Mean MPO values in normal individuals were 340 +/- 98 ng/ml in EDTA plasma (n = 152) and 332 +/- 82 ng/ml in heparinized plasma (n = 34). When MPO was measured 12-6 h after injury in critically ill patients high values (above 1000 ng/ml) were found in 6/15 patients with multiple injuries. In patients with sepsis (n = 22), MPO values were always above 1000 ng/ml. [less ▲]

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See detailMyeloperoxidase and elastase as markers of leukocyte activation during cardiopulmonary bypass in humans
Faymonville, Marie ULg; Pincemail, Joël ULg; Duchateau, J. et al

in Journal of Thoracic and Cardiovascular Surgery (The) (1991), 102

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See detailActivation of human immunodeficiency virus type 1 by an oxidative stress
Legrand-Poels, Sylvie ULg; VAIRA, Dolorès ULg; PINCEMAIL, Joël ULg et al

in AIDS Research and Human Retroviruses (1990)

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See detailTocopherol mobilization during dynamic exercise after beta-adrenergic blockade
Camus, G.; Pincemail, Joël ULg; Roesgen, Alice ULg et al

in Archives Internationales de Physiologie et de Biochimie (1990), 98

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See detailEquine postanaesthetic myositis: thromboxanes, prostacyclin and prostaglandin E2 production
Serteyn, Didier ULg; Deby, Ginette ULg; Pincemail, Joël ULg et al

in Veterinary Research Communications (1988), 12

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See detailThromboxane and prostacyclin release in adult respiratory distress syndrome
Deby, Ginette ULg; Braun, M.; Lamy, Maurice ULg et al

in Intensive Care Medicine (1987), 13

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See detail1019-Pentane measurement in man as an index of lipoperoxidation
Pincemail, Joël ULg; Deby, C.; Dethier, A. et al

in Bioelectrochemistry and Bioenergetics (Lausanne, Switzerland) (1987), 18

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See detailAction antiradicalaire de flavonoïdes et d'extraits de Chrysanthellum indicum
Brasseur, Thierry; Angenot, Luc ULg; Pincemail, Joël ULg et al

in Plantes médicinales et phytothérapie (1987), 21(2), 131-137

Chrysanthellum indicum DC subsp. americanum ( sold under the name of Chrysanthellum americanum) shows an antiradicalar activity against diphenylpicrylhydrazyl radical (DPPH). the activity seems to be ... [more ▼]

Chrysanthellum indicum DC subsp. americanum ( sold under the name of Chrysanthellum americanum) shows an antiradicalar activity against diphenylpicrylhydrazyl radical (DPPH). the activity seems to be mainly due to unusual flavonoids (e.g. mareine ( a chalcone) and maritimein ( an aurone) .Dilute alcoholic extracts would not be used for extract preparations because the glycosides are rapidly hydrolysed. [less ▲]

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See detailLa troxérutine et la rutine sulfate sodique possèdent-elles encore les propriétés de la rutine?
Brasseur, Thierry; Angenot, Luc ULg; Pincemail, Joël ULg et al

in Annales Pharmaceutiques Françaises (1987), 45(4), 329-334

Rutin, sodium rutin sulfate and troxerutin were tested for their antiradicalar, antilipoperoxidant, antioxygen properties and for their activity on in vivo prostaglandin biosynthesis.In each test, rutin ... [more ▼]

Rutin, sodium rutin sulfate and troxerutin were tested for their antiradicalar, antilipoperoxidant, antioxygen properties and for their activity on in vivo prostaglandin biosynthesis.In each test, rutin is more powerful than its derivatives. This fact results mainly on a free orthodiphenolic group ( 3'-4'-hydroxyls on ring B . [less ▲]

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See detailPropriétés antiradicalaires, antilipoperoxydantes et antioxydantes de flavonoïdes
Brasseur, Thierry; Angenot, Luc ULg; PINCEMAIL, Joël ULg

Poster (1986, October)

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See detailTrypsin-like activity and thromboxane release in adult respiratory distress syndrome.
Deby-Dupont, Ginette; Pincemail, Joël ULg; Faymonville, Marie-Elisabeth ULg et al

in Prostaglandins (1986), 32(1), 106-10

Plasmatic immunoreactive trypsin (IRT), thromboxane and trypsin-like enzymatic activity were measured in 117 patients at risk of developing adult respiratory distress syndrome (ARDS) (53 multiple injury ... [more ▼]

Plasmatic immunoreactive trypsin (IRT), thromboxane and trypsin-like enzymatic activity were measured in 117 patients at risk of developing adult respiratory distress syndrome (ARDS) (53 multiple injury, 30 abdominal surgery, 17 acute pancreatitis, 12 burnt and 5 disseminated intravascular coagulation patients). 69 of these patients developed ARDS. Immunoreactive trypsin and thromboxane were measured by radio-immuno-assay and trypsin-like enzymatic activity by spectrophotometry, using a specific chromogenic substrate. Mean IRT value was 675 ng/ml in ARDS and 265 ng/ml in non ARDS patients (p less than 0.05). Mean IRT value was 685 ng/ml in septic and 170 ng/ml in non septic patients (p less than 0.01). An abnormal trypsin-like enzymatic activity was measured in 26 ARDS patients. In 60 patients (37 ARDS and 23 non ARDS), thromboxane appeared in plasma simultaneously or about 24 hours after the beginning of IRT release. The importance of thromboxane release parallels the intensity of IRT. Originating from pancreas, trypsin can appear in plasma either by absorption from gastrointestinal tract or after pancreatic ischemia. [less ▲]

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See detailPropriétés antiradicalaires, antilipoperoxydantes et antioxydantes de flavonoïdes
Brasseur, Thierry; Angenot, Luc ULg; Pincemail, Joël ULg et al

in Bulletin de Liaison- Groupe Polyphenols (1986)

Nos résultats indiquent que le terme antioxydant ne peut plus être employé au sens large et montrent le rôle considérable que jouent les fonctions phénoliques des cycles B et C et la double liaison du ... [more ▼]

Nos résultats indiquent que le terme antioxydant ne peut plus être employé au sens large et montrent le rôle considérable que jouent les fonctions phénoliques des cycles B et C et la double liaison du cycle C dans l'activité des flavonoïdes. [less ▲]

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See detailBiochemical patways of acute lung injury
Lamy, Maurice ULg; Deby, Ginette ULg; Pincemail, Joël ULg et al

in Bulletin Européen de Physiopathologie Respiratoire (1985), 21

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See detailImmunoreactive trypsin in the adult respiratory distress syndrome
Deby, Ginette ULg; Haas, M.; Pincemail, Joël ULg et al

in Intensive Care Medicine (1984), 10

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