References of "Piette, Jacques"
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See detailAn in vivo model of varicella-zoster virus latent infection of dorsal root ganglia
Sadzot-Delvaux, Catherine ULg; Merville, Marie-Paule ULg; Delrée, P. et al

in Journal of Neuroscience Research (1990), 26(1), 83-89

We describe here the first in vivo model of varicella-zoster virus (VZV) latent infection in the adult rat peripheral nervous system. Infected Mewo cells were injected subcutaneously along the spine of ... [more ▼]

We describe here the first in vivo model of varicella-zoster virus (VZV) latent infection in the adult rat peripheral nervous system. Infected Mewo cells were injected subcutaneously along the spine of healthy adult rats. No clinical sign of infection was observed even 9 months after inoculation. Humoral immune response to VZV was detected in all infected animals throughout the study (9 months). The presence of viral material in dissociated and cultured dorsal root ganglia (DRG) from inoculated animals was studied by immunoperoxidase and in situ hybridization. When DRGs from infected animals were plated in culture from 1 month and up to 9 months after inoculation, viral nucleic acids and proteins were detected in neurons. Furthermore, trypsinization and subcultivation of infected neurons in culture is needed to reactivate infectious virus at least in some of the neurons. This model provides a useful tool for studying 1) the molecular mechanisms leading to an in vivo latency, 2) the role of the immune system, in particular cellular immunity, on the establishment, maintenance, and reactivation of latency, 3) the neurotropism of mutant viruses, and 4) the effects of antiviral agents. [less ▲]

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See detailAcute and persistent varicella-zoster virus infection of human and murine neuroblastoma cell lines
Bourdon-Wouters, C.; Merville, Marie-Paule ULg; Sadzot-Delvaux, Catherine ULg et al

in Journal of Neuroscience Research (1990), 26(1), 90-97

Human and murine neuroblastoma cell lines were infected in vitro with varicella-zoster virus (VZV). Infected human neuroblastoma cells (IMR-32) supported the synthesis of abundant viral antigens as ... [more ▼]

Human and murine neuroblastoma cell lines were infected in vitro with varicella-zoster virus (VZV). Infected human neuroblastoma cells (IMR-32) supported the synthesis of abundant viral antigens as detected by indirect immunoperoxidase labeling using human serum rich in anti-VZV antibodies and did not survive the infection. In situ hybridization (ISH) with VZV-cloned probes revealed a strong hybridization signal in these infected cells. During cultivation, the virus was released in the culture medium, and viral polypeptides were revealed by Western blotting of infected cells, using either a monoclonal anti-gpI antibody or a rabbit antiserum. All these findings indicate that IMR-32 cells support a productive and lytic infection by VZV, whether infected by cell-free virus or by cocultivation with infected cells. Murine neuroblastoma cells (neuro-2A) survived VZV infection and did not produce any infectious virus. No VZV-specific proteins were detected in infected cells either by immunolabeling or by Western blotting. However, viral nucleic acids could be detected by ISH, indicating that mouse neuroblastoma cells displayed a nonproductive, nonlytic infection. Infected neuro-2A cells have been examined by ISH using probes corresponding to immediate early (IE) genes 4, 62, and 63 and late (L) gene 31 encoding gpII. A strong hybridization signal was detected when infected cells were probed with a fragment containing the IE genes 62 and 63. Lower levels of hybridization were detected with the other probes, corresponding to IE or L genes. These systems allow comparative molecular analysis of persistent and acute infection of nerve cells by VZV. [less ▲]

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See detailTyping of human papillomaviruses in genital specimens by DNA hybridization
Lauricella, M.-A.; Piette, Jacques ULg; Lifrange, Eric ULg et al

in Archives Internationales de Physiologie et de Biochimie (1990), 98(2), 34

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See detailActivation of human immunodeficiency virus type 1 by an oxidative stress
Legrand-Poels, Sylvie ULg; VAIRA, Dolorès ULg; PINCEMAIL, Joël ULg et al

in AIDS Research and Human Retroviruses (1990)

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See detailReactivation of HIV-1 after an oxidative stress
Piette, Jacques ULg; Legrand-Poels, Sylvie; Vaira, Dolorès ULg et al

in Free Radicals in Biology and Medicine (1990), 9(Suppl. A), 5

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See detailDe la photophysique des molécules aromatiques en solution aux mécanismes de leurs effets biologiques
Piette, Jacques ULg; Hoebeke, Maryse ULg; Seret, Alain ULg

in Physicalia Magazine (1990), 12

Les études des interactions entre la lumière et la matière relèvent du domaine de la physique mais elles ont des implications importantes en chimie et en biologie. Dans cet article, nous présenterons ... [more ▼]

Les études des interactions entre la lumière et la matière relèvent du domaine de la physique mais elles ont des implications importantes en chimie et en biologie. Dans cet article, nous présenterons trois exemples, dans lesquels le phénomène de l'absorption lumineuse par certains chromophores et les réactions photochimiques, initiées à partir des différents états excités, constituent les étapes primaires de réactions photobiologiques qui peuvent avoir des conséquences aussi diverses que l'apparition de mutations dans les chromosomes et l'oxydation des constituants de membranes. Ces réactions biologiques sont appelées réactions de photosensibilisation et elles ont des implications importantes dans de nombreux domaines des sciences fondamentales et appliquées. [less ▲]

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See detailRose Bengal triplet state behaviour in sodium dodecyl sulfate micelles
Seret, Alain ULg; Piette, Jacques ULg; Van de Vorst, Albert

Poster (1989, August)

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See detailIntérêt du typage viral des papillomavirus humains (HPV) en relation avec les neoplasies des muqueuses genitales
Lifrange, Eric ULg; Lauricella, M.; Rentier, Bernard ULg et al

in Revue Médicale de Liège (1989), 44(1), 1-14

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See detailVaricella-zoster virus infection of adult rat sensory neurons in vitro.
Merville-Louis, M. P.; Sadzot-Delvaux, Catherine ULg; Delree, P. et al

in Journal of Virology (1989), 63(7), 3155-60

We report here an in vitro model of neuronal infection by varicella-zoster virus (VZV). Such a model has been achieved by using dissociated adult rat dorsal root ganglia cells infected by cocultivation ... [more ▼]

We report here an in vitro model of neuronal infection by varicella-zoster virus (VZV). Such a model has been achieved by using dissociated adult rat dorsal root ganglia cells infected by cocultivation with VZV-infected MRC5 cells or with cell-free virus. Indirect VZV immunolabeling, in situ hybridization, and neuron-specific immunolabeling demonstrated that VZV infection occurred selectively in neurons. VZV-specific immunolabeling detected a few neurons 1 or 2 days postinfection but not later. Genome detection using cloned VZV DNA probes revealed a hybridization signal primarily with RNA. Within 1 to 6 days postinfection, a progressive increase of VZV-specific hybridization was observed in up to 50% of the neurons. RNAs corresponding to immediate-early, early, and late genes were found, and transcripts of immediate-early gene 63 were particularly abundant. [less ▲]

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See detailImmunomodulation of varicella-zoster virus antigens
Marc, Ph.; Sadzot-Delvaux, Catherine ULg; Merville-Louis, M. P. et al

Poster (1989)

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See detailIn vivo model of varicella-zoster virus latency in the nervous system
Sadzot-Delvaux, Catherine ULg; Merville-Louis, Marie Paule; Delrée, P. et al

Conference (1989)

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See detailLatent infection in vivo by the varicella-zoster virus (VZV) in the rat nervous system
Sadzot-Delvaux, Catherine ULg; Merville, Marie-Paule ULg; Delrée, P. et al

in Archives Internationales de Physiologie et de Biochimie (1989), 97

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See detailAntibodies to varicella-zoster virus modulate antigen distribution in cultured infected cells
Marc, Ph.; Sadzot-Delvaux, Catherine ULg; Merville, Marie-Paule ULg et al

in Archives Internationales de Physiologie et de Biochimie (1989), 97

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See detailVaricella-Zoster virus infection in the nervous system: in vitro and in vivo models
Sadzot-Delvaux, Catherine ULg; Merville, Marie-Paule; Bourdon-Wouters, Christine et al

in Acta Neurologica Belgica (1989), 88

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See detailSinglet Oxygen Production and Photoisomerization: Two Competitive Processes for Merocyanine 540 Irradiated with Visible Light
Hoebeke, Maryse ULg; Seret, Alain ULg; Piette, Jacques ULg et al

in Journal of Photochemistry and Photobiology B : Biology (1988), 1(4), 437-446

The quantum yields of singlet oxygen production by merocyanine 540 have been measured during visible light irradiation performed in methanol and ethanol. These appear to be one hundred times smaller than ... [more ▼]

The quantum yields of singlet oxygen production by merocyanine 540 have been measured during visible light irradiation performed in methanol and ethanol. These appear to be one hundred times smaller than the quantum yield for rose bengal measured under the same conditions. Flash photolysis experiments demonstrate the ability of merocyanine 540 molecules to isomerize under visible light irradiation: the isomerization quantum yields are about 0.65 in both ethanol and methanol. This information combined with the fluorescence quantum yield data account for the low values for singlet oxygen production. [less ▲]

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See detailPersistent varicella-zoster virus infection in the nervous system: in vitro and in vivo models
Sadzot-Delvaux, Catherine ULg; Merville, Marie-Paule ULg; Bourdon-Wouters, C. et al

in Acta Neurologica Belgica (1988), 88

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See detailIn vitro neuroblastoma cell infection by varicella-zoster virus
Bourdon-Wouters, C.; Merville, Marie-Paule ULg; Piette, Jacques ULg et al

Poster (1988)

Detailed reference viewed: 5 (0 ULg)