References of "Pierard, Gérald"
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See detailFractal Quantification of the Microvasculature Heterogeneity in Cutaneous Melanoma
Heymans, O.; Blacher, Silvia ULg; Brouers, François ULg et al

in Dermatology : International Journal for Clinical & Investigative Dermatology (1999), 198(2), 212-7

BACKGROUND: Recent advances in angiogenesis research have led to the introduction of new prognosis factors. Although the vessel count is effective in breast cancer, the prognosis of primary cutaneous ... [more ▼]

BACKGROUND: Recent advances in angiogenesis research have led to the introduction of new prognosis factors. Although the vessel count is effective in breast cancer, the prognosis of primary cutaneous melanoma (PCM) does not seems to be directly influenced by this parameter. However, the microscopic examination of PCM suggests variability in the repartition of the microvasculature. OBJECTIVE AND METHOD: To explore the possibility of extracting information about the vessel distribution by performing a textural analysis on the grey level of histological sections by means of fractal characterization by both Fourier spectrum and multifractal analysis. RESULTS: Three different patterns of vasculature were identified according to the vessel density and distribution. CONCLUSION: It is possible to differentiate and quantify clearly the differences in the microvessel profile organization using the fractal and multifractal methods. [less ▲]

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See detailImmunohistochemical study of the rectus sheath and transversalis facia in adult groin hernias
PANS, Alain ULg; Pierard, Gérald ULg; Albert, Adelin ULg

in Hernia : the Journal of Hernias & Abdominal Wall Surgery (1999), 3

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See detailHumoral and cellular immune response to a crude exo-antigen and purified keratinase of Microsporum canis in experimentally infected guinea pigs.
Mignon, Bernard ULg; Leclipteux, T.; Focant, Charles ULg et al

in Medical Mycology (1999), 37(2), 123-129

In order to understand better the host-parasite relationship and to compare with previous observations in Microsporum canis naturally infected cats, the humoral and cellular immune responses to both a ... [more ▼]

In order to understand better the host-parasite relationship and to compare with previous observations in Microsporum canis naturally infected cats, the humoral and cellular immune responses to both a crude exo-antigen and a 31.5 kDa purified keratinase were evaluated in 12 M. canis experimentally infected guinea pigs. Humoral and cellular responses were assessed by ELISA from days 0 to 56 postinfection (PI) and by measurement of delayed-type hypersensitivity (DTH) responses on days 14 and 57 PI, respectively. Additionally, immunohistochemical staining was performed and demonstrated that the keratinase was produced in infected guinea pig skin, as previously reported in cats. Despite a marked interindividual variation, all the guinea pigs produced specific IgG to the crude exo-antigen from day 21 PI onwards, but no anti-keratinase IgG was detected. Strongly positive DTH responses to the exo-antigen were observed on both dates, whereas the keratinase elicited no and weak DTH on days 14 and 57 PI, respectively. These results are in agreement with those previously described for naturally infected cats, and indicate that the 31.5 kDa keratinase is not a major antigen in M. canis infection. [less ▲]

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See detailMorphotypes et postures de vie et de mort chez les trilobites.
Pierard, Sébastien ULg; Franchimont, Claudine ULg; Pierard, Gérald ULg

in Revue Verviétoise d'Histoire Naturelle (1999), 56

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See detailLe zona chez l'enfant
Nikkels, Arjen ULg; Tassoudji, Nazli ULg; Pierard, Gérald ULg

in Skin (1999), 2(1), 7-8

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See detailLes mélanomes cutanés, un spectre de cancers émergents chez la femme en région wallonne
Pierard-Franchimont, Claudine ULg; Arrese Estrada, Jorge ULg; Cornil, F. et al

in Revue Médicale de Liège (1999), 54(5), 495-499

The Mosan Study Group of Pigmented Neoplasms was founded about 15 years ago. It has collected more than 20.000 cutaneous malignancies including melanoms and basal and squamous cell carcinomas. The ... [more ▼]

The Mosan Study Group of Pigmented Neoplasms was founded about 15 years ago. It has collected more than 20.000 cutaneous malignancies including melanoms and basal and squamous cell carcinomas. The incidence of these cancers in on the rise in Wallonia. In particular, malignant melanoms represent a spectrum of emerging cancers characterized by a proteiform biological outcome. They mostly affect young women. The major risk factor appears to be iterative and unwise ultraviolet exposures. The prevention of malenomas is basically founded on such a dogma and accordingly relies on sunscreens. However, controversies about their beneficial effects are rife and fueled by axiomas and contradictory sophisms. At the exception of surgery, the therapeutic options for the diverse types of melanomas do not yet fulfill the scope of evidence-based medicine. [less ▲]

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See detailSimultaneous Reactivation of Herpes Simplex Virus and Varicella-Zoster Virus in a Patient with Idiopathic Thrombocytopenic Purpura
Nikkels, Arjen ULg; Frere, P.; Rakic, L. et al

in Dermatology : International Journal for Clinical & Investigative Dermatology (1999), 199(4), 361-364

Simultaneous reactivation of distinct Herpesviridae with development of clinical manifestations is exceptional. We report a 48-year-old woman suffering from idiopathic thrombocytopenic purpura. As the ... [more ▼]

Simultaneous reactivation of distinct Herpesviridae with development of clinical manifestations is exceptional. We report a 48-year-old woman suffering from idiopathic thrombocytopenic purpura. As the disease remained refractory to corticosteroids, immunoglobulins and splenectomy, a cure of vinblastine was administered. An atypical stomatitis developed few days later. Immunohistochemistry on a Tzanck smear and a biopsy evidenced a Herpes simplex virus type 1 (HSV-1) infection. The patient presented simultaneously a single necrotic lesion on the abdomen. Immunohistochemistry on a skin biopsy revealed the presence of the varicella-zoster virus (VZV) gE, gB and IE63 proteins. Intravenous aciclovir was initiated. The present case of simultaneous clinical infections by HSV-I and VZV underlines the importance of complementary viral identification testing in the event of unusual clinical presentations. [less ▲]

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See detailPurification and Characterization of a 315 Kda Keratinolytic Subtilisin-Like Serine Protease from Microsporum Canis and Evidence of Its Secretion in Naturally Infected Cats
Mignon, Bernard ULg; Swinnen, M.; Bouchara, J. P. et al

in Medical Mycology (1998), 36(6), 395-404

A keratinolytic protease, secreted as the major component by a feline clinical isolate of Microsporum canis cultivated in a minimal medium containing cat keratin, was purified by affinity chromatography ... [more ▼]

A keratinolytic protease, secreted as the major component by a feline clinical isolate of Microsporum canis cultivated in a minimal medium containing cat keratin, was purified by affinity chromatography on bacitracin agarose and gel filtration. The apparent molecular mass of the enzyme was 31.5 kDa and the pI was 11.8. The enzyme was not glycosylated and its first 15 N-terminal amino acids showed numerous similarities with other fungal subtilisins. The optimum pH was around 9 while inactivation of the enzyme was reversible at pH 4, but not at pH 11. The enzyme was stable at 37 degrees C with an apparent optimum temperature around 55 degrees C. PMSF, soybean trypsin inhibitor (SBTI) and chymostatin strongly inhibited the proteinase. The highest affinity (Km of 0.37 mM) and physiological efficiency (k(cat)/Km) were obtained for the synthetic substrate N-Suc-Ala-Ala-Pro-Phe-p-nitroanilide. These results indicate that the keratinase belongs to the subtilisin-like serine protease family. Purified rabbit immunoglobulins G prepared against the keratinase and used in an immunohistochemical test allowed the detection of the keratinase produced by the fungus invading hair structures in naturally infected cats. The in vitro keratinolytic activity of the enzyme and its production in vivo suggest that it may contribute to pathogenicity. [less ▲]

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See detailPatterns of the Immunohistochemical Expression of Melanoma-Associated Antigens and Density of Cd45r0+ Activated T Lymphocytes and L1-Protein Positive Macrophages in Primary Cutaneous Melanomas
Pierard-Franchimont, Claudine ULg; Letawe, C.; Nikkels, Arjen ULg et al

in International Journal of Molecular Medicine (1998), 2(6), 721-724

There is ample evidence for spontaneous antimelanoma immune reactivity mediated by melanocyte-differentiation-antigens (MDAs). Our aim was to determine whether MDA immunoreactivity is associated with ... [more ▼]

There is ample evidence for spontaneous antimelanoma immune reactivity mediated by melanocyte-differentiation-antigens (MDAs). Our aim was to determine whether MDA immunoreactivity is associated with increased tumour-infiltrating lymphocytes (TIL) and macrophages (TIM). A retrospective study was conducted in 30 medium and high grade primary cutaneous melanomas (PCM) as identified by CART-analysis. All of the cases had developed clinical evidence for metastasis within 3 years following surgical excision of the PCM. We used immunohistochemistry and computerized image analysis to quantify MDAs positive cells (Melan A/MART-1, gp100/Pmel 17/HMB45, tyrosinase), CD45R0-positive TIL and LI-protein-positive TIM. A stochastic relationship was present between the MDA immuno-reactivities and the densities in TIL and TIM. An inverse relationship was yielded between TIL and TIM. No specific pattern of PCM immunoreactivity for MDAs, TIL and TIM was found to predict metastases. [less ▲]

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See detailPharma clinics. Comment je traite ... ou préviens une douleur par "patches"
Pierard, Gérald ULg

in Revue Médicale de Liège (1998), 53(10), 586-7

There exists two distinct types of patches aiming at increasing the pain threshold either systematically or locally. The first type of analgesic patch is a real transdermal delivery system releasing ... [more ▼]

There exists two distinct types of patches aiming at increasing the pain threshold either systematically or locally. The first type of analgesic patch is a real transdermal delivery system releasing fentanyl. Such potent opioid drug exerts a systemic effect. The other type of patch associates lidocain and prilocain to induce a local anesthesia of the skin without inducing a systemic effect. [less ▲]

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See detailZoonoses cutanées transmises par les chiens et les chats
Pierard, Gérald ULg; Pierard-Franchimont, Claudine ULg; Arrese Estrada, Jorge ULg et al

in Revue Médicale de Liège (1998), 53(9), 532-536

Pets like dogs and cats are responsible for a series of zoonotic disorders. Some of these diseases are inflicted by bites, scratches or licking. Others result from a close contact with fur, or are ... [more ▼]

Pets like dogs and cats are responsible for a series of zoonotic disorders. Some of these diseases are inflicted by bites, scratches or licking. Others result from a close contact with fur, or are transmitted by ectoparasites or by the contaminated environment. Some systemic infectious and parasitic diseases are transmitted from animals to humans and vice versa. Such situation may lead to endemic problems in some communities. [less ▲]

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See detailRelativité et géodiversité des vérités médico-économiques
Pierard, Gérald ULg

in Revue Médicale de Liège (1998), 53(5), 252-4

The economic gestion of health care is difficult to handle. It covers several aspects which cannot pride to be accurate sciences. It strikes difficulties such as the geodiversity of problems to be solved ... [more ▼]

The economic gestion of health care is difficult to handle. It covers several aspects which cannot pride to be accurate sciences. It strikes difficulties such as the geodiversity of problems to be solved and the nuances in the application of principles of social equity and medical ethics. The health-care analysis is also exposed to some politician interpretations biased by doctrinal a priori. Most often, a confrontation ensues between the political cenacle and the professionals directly involved in health-care. [less ▲]

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See detailLe coût des diagnostics empiriques et intuitifs. Regard critque sur l'épreuve thérapeutique et sur les pratiques non conventionnelles
Pierard, Gérald ULg

in Revue Médicale de Liège (1998), 53(5), 249-51

Some health care-related practices are probably on the rise as a response to the political economy. In particular, the therapeutic prescription looking for a diagnostic information allows to escape from a ... [more ▼]

Some health care-related practices are probably on the rise as a response to the political economy. In particular, the therapeutic prescription looking for a diagnostic information allows to escape from a possible overcharge linked to proper diagnostic methods. The cost of such practice is not negligible. In fact, the best way to handle the problem is to reinstate some simple and cheap diagnostic tests in the daily practice of clinicians. The opening of the health care disciplines to new categories of non-physician workers is about to create further problems with regard to the health economics. The unsettled diagnosis and the therapeutic limitation to a quasi-placebo effect will never be positive factors in the global gestion of pathologies. [less ▲]

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See detailComment je traite ... un phénomène de Raynaud
Pierard, Gérald ULg

in Revue Médicale de Liège (1998), 53(3), 119-20

Raynaud's phenomenon is a paroxysmal vasospasm affecting the extremities, particularly the fingers. The idiopathic type is the most frequently encountered and the secondary types have multiple origins ... [more ▼]

Raynaud's phenomenon is a paroxysmal vasospasm affecting the extremities, particularly the fingers. The idiopathic type is the most frequently encountered and the secondary types have multiple origins. The mainstay of treatment relies on hygieno-dietetic measures. When they fail, calcium antagonists, sustained-release transdermal glyceryl trinitrate and a few other vasoactive drugs may prove to be effective. However, side effects may limit their prescription. [less ▲]

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See detailAnneaux, cibles ou cocardes
Pierard-Franchimont, Claudine ULg; Nikkels, Arjen ULg; Arrese Estrada, Jorge ULg et al

in Revue Médicale de Liège (1998), 53(3), 117-118

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See detailMalignant Melanoma Staging Using Whole-Body Positron Emission Tomography
Paquet, Philippe ULg; Hustinx, Roland ULg; Rigo, Pierre ULg et al

in Melanoma Research (1998), 8(1), 59-62

The correct staging of potentially metastatic melanoma is essential for an appropriate therapeutic attitude. Current methods include computed tomography, magnetic resonance imaging, ultrasonography and ... [more ▼]

The correct staging of potentially metastatic melanoma is essential for an appropriate therapeutic attitude. Current methods include computed tomography, magnetic resonance imaging, ultrasonography and scintigraphy. Another tool is whole-body positron tomography using the radiopharmaceutical 2-fluorine-18-fluoro-2-deoxy-D-glucose as an emitter because it accumulates inside neoplasms, especially melanoma. We report two cases of malignant melanoma in which computed tomography and magnetic resonance imaging suggested visceral metastatic spread. In contrast, whole-body positron emission tomography indicated the absence of metastases, and this was confirmed by histological examination of the organs where metastases were suspected. Whole-body positron emission tomography appears to have high specificity and sensitivity for clinical melanoma staging. [less ▲]

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See detailLa couverture 1998 de la Revue medicale de Liège: histoplasmose chez un sidéen
Pierard, Gérald ULg

in Revue Médicale de Liège (1998), 53(1), 2-3

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See detailL'image du mois. Acne gymnasium: une acne fulminante dopee.
Pierard, Gérald ULg

in Revue medicale de Liege (1998), 53(8), 441-3

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See detailL1-protein in incipient lymphocyte-depleted graft-versus-host disease. Expression in keratinocytes and coexpression with Factor XIIIa in dermal cells
Pierard, Gérald ULg; Tassoudji, Nazli ULg; Arrese Estrada, Jorge ULg et al

in Turkish Journal of Dermatology = Dermatopatoloji Dergisi (1998), 7

With the introduction of effective immunosuppressive regimens some cutaneous rashes following bone marrow transplant may appear histologically unremarkable. We report three cases of cyclosporin-abated ... [more ▼]

With the introduction of effective immunosuppressive regimens some cutaneous rashes following bone marrow transplant may appear histologically unremarkable. We report three cases of cyclosporin-abated cutaneous graft-versus-host reaction (GVHR) where the most straightful histologic clue was the epidermal L1-protein (calprotectin) expression revealed by a positive immunolabelling using the Mac 387 antibody. The epidermis looked otherwise normal and inflammatory cells were scant in the dermis. The expression of calprotectin by keratinocytes occurred early in cutaneous GVHR. In addition, CD45RO-positive T memory lymphocytes were exceedingly rare in the dermis while a few Mac 387-positive monocytes coexpressed Factor XIIIa immunoreactivity. Hyperplasia of the Factor XIIIa-positive dendrocytic population was obvious. Calprotectin and Factor XIIIa immunolagellings should be regarded as a diagnostic adjunct in lymphocyte-depleted cutaneous GVHR. Calprotectin positivity of the epidermis is indicative although not specific for such condition. However, its combination with a low lymphocytes/monocytes ratio in the dermis which is enriched in plup dendrocytes is almost diagnostic. [less ▲]

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See detailEpidermal Calprotectin Expression in Lymphocyte-Depleted Cutaneous Graft-versus-Host Reaction
Pierard, Gérald ULg; Nikkels, Nazli ULg; Nikkels, Arjen ULg et al

in Archivos Argentinos de Dermatologia (1998), 48

One of the most important complications associated with bone marrow transplantation (BMT) is graft-versus-host reaction (GVHR) altering different organs. The immunosuppressive regimen frequently abates ... [more ▼]

One of the most important complications associated with bone marrow transplantation (BMT) is graft-versus-host reaction (GVHR) altering different organs. The immunosuppressive regimen frequently abates the severity of cutaneous lesions to a peculiar lymphocyto-depleted GVHR (LD-GVHR) with scant recognizable inflammatory cells and almost absence of epidermal injury. The recently revisited histological criteria for cutaneous GVHR are of little help in diagnosing such LD-GVHR. As calprotectin (L1-protein) has been reported to be expressed in several types of stressed epithelia, we assessed the epidermal calprotectin expression during LD-GVHR. Calprotectin expression was studied by immuno-histochemistry using the Mac 287 moAb in 50 cases of LD-GVHR and 40 cases of toxic reactions due to the conditioning regimens or to post-transplant drugs. Calprotectin was evidenced in normal looking keratinocytes of all cutaneous LD-GVHR cases and in the vast majority of cytotoxic drug-induced dermatitis. It is concluded that calprotectin immunoreactivity appears to be a diagnostic clue in LD-GVHR. The epidermal calprotectin expression occurs early in GVHD, irrespective of the histological grading. However, it cannot be used alone to distinguish early LD-GVHR from drug-induced dermatitis. [less ▲]

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