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Showing results 161 to 176 of 176
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Tilapia growth hormone: molecular cloning of cDNA and expression in Escherichia coliRentier-Delrue, Françoise  ; Swennen, D.; Philippart, Jean-Claude  et al in DNA (1989), 8(4), 271-8 A cDNA library was prepared from poly(A)+RNA extracted from tilapia Oreochromis niloticus anterior pituitaries. The recombinant clones carrying the cDNA sequence of tilapia growth hormone (tiGH) were ... [more ▼] A cDNA library was prepared from poly(A)+RNA extracted from tilapia Oreochromis niloticus anterior pituitaries. The recombinant clones carrying the cDNA sequence of tilapia growth hormone (tiGH) were selected using a fragment of the trout growth hormone (tGH) cDNA as hybridization probe. The nucleotide sequence of the full-length tiGH cDNA was determined. This cDNA encodes a protein of 204 amino acids, including the putative signal peptide of 17 amino acids. Mature tiGH cDNA was inserted in an Escherichia coli expression vector which led to the production of tiGH protein with a yield estimated to be 20% of the total bacterial proteins. [less ▲] Detailed reference viewed: 33 (7 ULg)
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