References of "Peulen, Olivier"
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See detailDifferential effect of dietary spermine on alkaline phosphatase activity in jejunum and ileum of unweaned rats
Peulen, Olivier ULg; Gharbi, Myriam ULg; Powroznik, Brigitte et al

in Biochimie (2004), 86(7), 487-493

Spermine is a low molecular weight polyamine involved in the postnatal maturation of the gut. When it is administered orally to suckling rats it induces the maturation of their spleen, liver, pancreas ... [more ▼]

Spermine is a low molecular weight polyamine involved in the postnatal maturation of the gut. When it is administered orally to suckling rats it induces the maturation of their spleen, liver, pancreas, and small intestine. We showed that this polyamine modulates differently the activity of alkaline phosphatase in jejunum and ileum in suckling rat. In 14-day-old rat which had received spermine orally for 3 days, once daily, an increase of alkaline phosphatase activity in the jejunum and a decrease of this activity in the ileum was observed. Alkaline phosphatase was located at the bottom of the villus in the control jejunum and in the whole length of the villus in spermine-treated rats. On the contrary, in ileum of controls, this enzyme was present in the whole length of the villus but disappeared in the spermine-treated animals. An enzyme mass shift was observed in the small intestine after spermine administration. Spermine administration did not change the expression of genes coding for alkaline phosphatase, suggesting a post-transcriptional modification. (C) 2004 Elsevier SAS. All rights reserved. [less ▲]

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See detailSpermine-induced maturation in Wistar rat intestine: A cytokine-dependent mechanism
Peulen, Olivier ULg; Dandrifosse, Guy ULg

in Journal of Pediatric Gastroenterology and Nutrition (2004), 38(5), 524-532

Objectives: Polyamines are of great importance in biologic processes such as cell proliferation and differentiation. The ingestion of spermidine or spermine by suckling rats induces the precocious ... [more ▼]

Objectives: Polyamines are of great importance in biologic processes such as cell proliferation and differentiation. The ingestion of spermidine or spermine by suckling rats induces the precocious maturation of the small intestine. In a previous article, the authors hypothesized that this phenomenon could be mediated by interleukins. This work was performed to examine the role of IL-1, IL-2, and tumor necrosis factor (TNF)-alpha in the spermine-induced maturation of the small intestine. Methods: Wistar suckling rats were treated with spermine, FR167653 (inhibitor of IL-1beta/TNF-alpha production), IL-1beta/TNF-alpha neutralizing antibodies, lipopolysaccharide, or IL-2. Intestinal disaccharidase-specific activities, polyamine content, and IL-2 plasma concentration were analyzed. Comparisons were made with untreated control animals. Results: Spermine-induced maturation of the small intestine was decreased by FR167653 but not by the neutralizing antibodies. Lipopolysaccharide injection induced an increase in disaccharidase-specific activity. IL-2 induced a decrease of the intestinal lactase-specific activity. Spermine administration led to a similar decrease of lactase activity and to an increase of IL-2 plasma concentration. Conclusions: The authors conclude that IL-1beta and TNF-alpha are involved in the spermine effects on maltase- and sucrase- specific activities and suggest that IL-2 is involved in the spermine-induced decrease of lactase activity. (C) 2004 Lippincott Williams Wilkins. [less ▲]

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See detailPolyamines in gut inflammation and allergy
Peulen, Olivier ULg; Deloyer, Patricia; Deville, Christelle ULg et al

in Current Medicinal Chemistry - Anti-Inflammatory & Anti-Allergy Agents (2004), 3

The natural polyamines, named 1,4-diaminobutane, N-aminopropyl-1,4-diaminobutane and N,N'-bisaminopropyl- 1,4-diaminobutane, are also designated respectively as putrescine, spermidine and spermine. They ... [more ▼]

The natural polyamines, named 1,4-diaminobutane, N-aminopropyl-1,4-diaminobutane and N,N'-bisaminopropyl- 1,4-diaminobutane, are also designated respectively as putrescine, spermidine and spermine. They are polycationic compounds found in all eukaryotic cells. As they are deeply involved in cell functions, e.g. cellular growth, their concentration and their equilibrium in the intracellular pool are maintained in very narrow limits by regulatory mechanisms acting in a very fast, sensitive and precise way. These compounds are involved in gut inflammatory processes and in allergy. Indeed, they control the production of inflammatory mediators in several cell lines or tissues. Polyamine metabolism could be considered as a putative target for inflammation, allergy prevention or therapy. [less ▲]

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See detailPotentialisation de l'immunité naturelle du rat nouveau-né par les polyamines exogènes
Powroznik, Brigitte; Gharbi, Myriam ULg; Dandrifosse, Guy ULg et al

Conference (2004)

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See detailPolyamine as innate immunity modulator
Peulen, Olivier ULg; Powroznik, Brigitte; Gharbi, Myriam ULg et al

in Pflügers Archiv : European Journal of Physiology (2004), 449

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See detailSpermine-induced modifications of intestinal urea cycle
Peulen, Olivier ULg; Gharbi, Myriam ULg; Powroznik, Brigitte et al

in Pflügers Archiv : European Journal of Physiology (2004)

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See detailShort-term effects of spermine ingestion on the small intestine: a comparison of suckling and weaned rats
Peulen, Olivier ULg; Deloyer, Patricia; Dandrifosse, Guy ULg

in Reproduction Nutrition Development (2004), 44(4, Jul-Aug), 353-364

We have previously shown that spermine, shortly after its ingestion, can induce the alteration of the morphology of the small intestine of suckling rats. It was proposed that this alteration is due to ... [more ▼]

We have previously shown that spermine, shortly after its ingestion, can induce the alteration of the morphology of the small intestine of suckling rats. It was proposed that this alteration is due to polyamine accumulation inside the epithelial cells. This could also be related to the fact that the intestine of the suckling rat is in an immature state. To shed light on this issue, disaccharidase and alkaline phosphatase activity assays, protein, DNA and RNA content measurements and polyamine concentration analysis were performed on the small intestine of suckling and weaned Wistar rats treated with spermine. Spermine did not induce the same intestinal alterations in weaned rats compared to suckling animals. Indeed, in sucklings, spermine administration induced a decrease of the protein, DNA, putrescine and spermidine intestinal content, suggesting a cell loss. The cell loss impaired the activity of intestinal enzymes: lactase, maltase and alkaline phosphatase. In weaned rats, the same treatment did not alter these parameters. Exogenous spermine by itself is not sufficient to induce the alterations described here and previously. The maturity degree of the small intestine could be the basis of this process. [less ▲]

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See detailEnhancement of lysozyme stability and activity by polyamines
Powroznik, Brigitte; Gharbi, Myriam ULg; Dandrifosse, Guy ULg et al

in Biochimie (2004), 86(9-10, Sep-Oct), 651-656

Spermine, a low molecular weight polyamine, administered orally to suckling rats induces the maturation of the small intestine. In this organ, lysozyme is an important component of the innate immunity. In ... [more ▼]

Spermine, a low molecular weight polyamine, administered orally to suckling rats induces the maturation of the small intestine. In this organ, lysozyme is an important component of the innate immunity. In this report, we analysed the binding of spermine to lysozyme and its effect on thermal inactivation of the protein by spectroscopy techniques. The activity of the enzyme was analysed in presence of spermine by lysoplate technique. We studied the effects of spermine ingestion by suckling rats on intestinal lysozyme activity and gene expression. We reported that spermine binds to lysozyme and increases in vitro the thermal stability and the activity of the protein. When administered orally to suckling rats, spermine increases the lysozyme activity in jejunum, but not in ileum. This increase is not due to a modification of the gene expression. The observed effects lead us to postulate that spermine could be used in some mammals as a promoter of the innate immunity. (C) 2004 Elsevier SAS. All rights reserved. [less ▲]

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See detailAge, sex, and weight at weaning influence organ weight and gastrointestinal development of weanling pigs
Pluske, J. R.; Kerton, D. J.; Cranwell, P. D. et al

in Australian Journal of Agricultural Research (2003), 54(5), 515-527

The present study was designed to determine the interrelationships between sex, weaning age, and weaning weight on aspects of physiological and gastrointestinal development in pigs. Forty-eight Large ... [more ▼]

The present study was designed to determine the interrelationships between sex, weaning age, and weaning weight on aspects of physiological and gastrointestinal development in pigs. Forty-eight Large White x Landrace pigs were used in a factorial arrangement with the respective factors being: age at weaning ( 14 or 28 days), weight at weaning ( heavy or light), sex ( boar or gilt), and time after weaning ( 1, 7, and 14 days). At weaning, 48 pigs were removed from the sow; 16 pigs were then fasted for 24 h before euthanasia for determination of organ weights, gut histology, and enzymology, and 32 pigs were offered a high quality pelleted weaner diet ad libitum for subsequent assessment of organ weights, histology, and enzymology at 7 and 14 days after weaning. On Day 6 and 13 after weaning, 2 pigs from each group had their feed removed, and 24 h later were euthanased and similar measurements were taken. In general, the data highlighted the overall gastrointestinal underdevelopment of pigs weaned at 2 weeks of age and of pigs weaned light-for-age at either 2 or 4 weeks. Heavier body organs, gastrointestinal organs, and accessory digestive organs observed after weaning, except for the spleen, presumably reflected the increase in substrates available for cellular growth as feed intake increased after weaning, and the development of organs required to process this feed. Interestingly, the relative weights (% of liveweight) of the stomach and small intestine and, to a lesser extent, the caecum and colon, were greater in the light, 14-day-old weaned pigs, but these differences diminished with increasing time after weaning. Consistent effects due to age, weight, and sex were not observed for villous height and crypt depth, or for the specific activities of the brush-border and pancreatic enzymes measured. However, increases (P < 0.001) in the activities of maltase (P < 0.001), glucoamylase ( P < 0.001), and sucrase (P = 0.020) ( all expressed per gram of mucosa), and that of trypsin ( per gram of pancreas), occurred by 14 days after weaning. This most likely reflected the inducible nature of these enzymes in response to the increasing intake of substrates provided in the diet. In contrast, the specific activity of lactase declined (P = 0.012) in the first 14 days after weaning. These data suggest that pigs weaned at 2 weeks of age and pigs weaned light-for-age at either 2 or 4 weeks have a less developed gastrointestinal tract, and that its development after weaning might proceed differently to that of pigs weaned older and heavier. [less ▲]

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See detailSpermine induces precocious development of the spleen in mice
Jolois, Olivier ULg; Peulen, Olivier ULg; Collin, Serge ULg et al

in Experimental Physiology (2002), 87(1), 69-75

Spermine is a low molecular weight polyamine involved in the postnatal maturation of the gut. When it is administered orally to suckling rats, it induces maturation of the intestinal tract (liver ... [more ▼]

Spermine is a low molecular weight polyamine involved in the postnatal maturation of the gut. When it is administered orally to suckling rats, it induces maturation of the intestinal tract (liver, pancreas and small intestine). Here we show that this polyamine is able to induce precocious intestinal and splenic development in suckling mice. In fact, in 15-day-old mice which had received spermine orally twice daily for 3 days we observed an increase in the ratio of white pulp surface to total spleen surface in comparison with untreated mice. The two macrophage subsets of the marginal zone and the B-cell population were more developed and reached the development level of 5- or 10-week-old mice. The proliferation rate of B-cells was increased by spermine administration to pups. These observations suggest that spermine might play a role in immune system development; further investigation of its effects are intended, namely the evaluation of its capacity to enhance defence during the neonatal period. [less ▲]

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See detailEffect of dietary polyamines and amino acids on the polyamine content of rat milk
Peulen, Olivier ULg; Dandrifosse, Guy ULg

in Morgan, D.; Hirvi, T.; Dandrifosse, Guy (Eds.) et al COST 917 Biologically active amines in food. Volume VI: Biologically active amines: metabolism and physiology and Biologically active amines in food processing and production of biologically active amines by bacteria (2002)

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See detailPolyamines as essential substances in metabolism
Dandrifosse, Anne-catherine; Deloyer, Patricia; Loret, Suzanne et al

in Trends in Comparative Biochemistry and Physiology (2002), 9

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See detailSpermine-Induced Alteration of Small Intestine in Suckling Rat: Involvement of Apoptosis or Zn2+ Enzymes?
Peulen, Olivier ULg; Denis, Ghislaine; Defresne, Marie-Paule ULg et al

in Digestive Diseases & Sciences (2001), 46(11), 2490-8

Polyamines are of great importance in several physiological processes, such as cell proliferation and differentiation. The ingestion of spermine by suckling rats induces precocious maturation of their ... [more ▼]

Polyamines are of great importance in several physiological processes, such as cell proliferation and differentiation. The ingestion of spermine by suckling rats induces precocious maturation of their small intestine. Shortly after ingestion, spermine produces cell elimination at the villous top. The origin of this exfoliation was investigated to determine whether it was due to apoptosis. Wistar rats were orally treated with spermine. Apoptosis was analyzed in their small intestine by Tdt-mediated dUTP-fluorescein nick-end labeling reaction, caspase-3-like analysis, and DNA laddering. Polyamine content was measured by HPLC. The intestinal transitory alteration appeared as soon as 2 hr after spermine administration. Apoptosis events increased strongly at the same moment in the small intestine. They were evidenced by Tdt-mediated dUTP-fluorescein nick-end labeling analysis, DNA laddering, and caspase-3-like activity. Changes observed are consistent with apoptosis, but caspase inhibitor did not reduce intestinal alteration, as did Zn2+ chelator. [less ▲]

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See detailDietary Polyamines and Non-Neoplastic Growth and Disease
Deloyer, Patricia; Peulen, Olivier ULg; Dandrifosse, Guy ULg

in European Journal of Gastroenterology & Hepatology (2001), 13(9), 1027-32

This review presents the data that are now available concerning the effects of dietary polyamines at either postnatal or adult stages in non-neoplastic growth and disease. Polyamines provided by food have ... [more ▼]

This review presents the data that are now available concerning the effects of dietary polyamines at either postnatal or adult stages in non-neoplastic growth and disease. Polyamines provided by food have a potential role in growth and development of the digestive system in neonatal mammals (and fishes). In humans, this property could be of importance in preventing the appearance of food allergies. Dietary polyamines also seem necessary for the maintenance of normal growth and general properties of adult digestive tract. Their possible therapeutic effects have been investigated in gastric, intestinal, and, more recently, whole-body healing. [less ▲]

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See detailSpermine induces precocious development of the spleen in Wistar rat
Peulen, Olivier ULg; Jolois, Olivier ULg; Galopin, Catherine et al

in Trends in Comparative Biochemistry and Physiology (2001), 8

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See detailMaturation of the small intestine is induced by spermine but not by other similar amines
Peulen, Olivier ULg; Grandfils, Christian ULg; Dandrifosse, Guy ULg

in Pflügers Archiv : European Journal of Physiology (2000, September 19), 440

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See detailCyclosporine a Inhibits Partially Spermine-Induced Differentiation but Not Cell Loss of Suckling Rat Small Intestine
Peulen, Olivier ULg; Dandrifosse, Guy ULg

in Digestive Diseases & Sciences (2000), 45(4), 750-4

The polyamines are of great importance in several biological processes, such as cell proliferation, and differentiation. The ingestion of spermine by suckling rats induces the precocious maturation of ... [more ▼]

The polyamines are of great importance in several biological processes, such as cell proliferation, and differentiation. The ingestion of spermine by suckling rats induces the precocious maturation of their small intestine. This phenomenon is preceded by a cell elimination at the villus tip. We hypothesize that these two phenomena could be mediated by the immune system and thus inhibited by an immunosuppressive agent such as cyclosporine A. Cyclosporine A inhibits, at least partially, the spermine-induced increase of the maltase- and sucrase-specific activities in the small intestine but failed to inhibit lactase-specific activity decrease and cell loss. Spermine does not act by the same mechanism in differentiation and in cell loss. Moreover, spermine acts in a different way on lactase-specific activity compared to maltase- or sucrase-specific activity. We hypothesize that spermine acts on differentiation by a T-cell/IL-2-dependent mechanism. [less ▲]

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See detailAre Milk Polyamines Preventive Agents against Food Allergy?
Dandrifosse, Guy ULg; Peulen, Olivier ULg; El Khefif, N. et al

in Proceedings of the Nutrition Society (2000), 59(1), 81-6

Insufficient polyamine intake could play a role in the induction of sensitization to dietary allergens. This proposal is based essentially on investigations made in sucking rats and in children. In ... [more ▼]

Insufficient polyamine intake could play a role in the induction of sensitization to dietary allergens. This proposal is based essentially on investigations made in sucking rats and in children. In sucking rats it has been established that oral administration of spermine can induce all the modifications occurring in the digestive tract at weaning. In the intestine events occur in two phases. The early event consists of desquamation of the epithelium resulting from an activation of apoptosis. The late event appears to involve an hormonal cascade in which adrenocorticotropic hormone, cytokines, bombesin and corticosterone are included. Observations in human subjects show that: (1) the spermine and spermidine concentrations are generally lower in infant formulas than in human breast milk. Mothers seem consistently to have relatively high or relatively low concentrations of spermine and spermidine in their milk. These individual variations may be due to diet, lifestyle or genetic background; (2) the probability of developing allergy can reach 80 % if the mean spermine concentration in the milk is lower than 2 nmol/ml milk. It is approximately 0 % if the mean spermine concentration is higher than 13 nmol/ml milk; (3) preliminary results show that the intestinal permeability to macromolecules differs in premature babies when they are fed on breast milk compared with infant formulas (J Senterre, J Rigo, G Forget, G Dandrifosse and N Romain, unpublished results). This difference does not seem to be present when powdered milk is supplemented with polyamines at the concentration found in breast milk; (4) spermine increases proliferation and differentiation of lymphocytes isolated from the tonsils of children. [less ▲]

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See detailAre there arguments suggesting that polyamines are preventive agents of food allergy ?
Dandrifosse, Guy ULg; Peulen, Olivier ULg; Khefif, N. El et al

in Proceeding of the COST 917 Meeting in Malaga (2000)

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