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See detailEffect of sampling method and incubation temperature on fungal culture in canine sino-nasal aspergillosis
Billen, Frédéric ULg; Clercx, Cécile ULg; Le Garérrès, Alain et al

in Journal of Small Animal Practice (2009), 50(2), 67-72

OBJECTIVES: To evaluate the most appropriate sampling procedure and the effect of incubation temperature on fungal culture in the diagnosis of canine sinonasal aspergillosis (SNA). METHODS: Sixteen dogs ... [more ▼]

OBJECTIVES: To evaluate the most appropriate sampling procedure and the effect of incubation temperature on fungal culture in the diagnosis of canine sinonasal aspergillosis (SNA). METHODS: Sixteen dogs with SNA and 20 dogs with non-fungal nasal disease entered a prospective study. Nasal secretions and mucosal biopsies were collected in all dogs. Fungal plaques were also sampled in dogs with SNA. Each specimen was taken in duplicate from each dog and incubated at room temperature and 37 degrees C. RESULTS: In dogs with SNA, nasal secretions, mucosal biopsies and fungal plaques yielded fungal growth at room temperature in one, one and seven dogs, respectively, whereas fungal growth was obtained at 37 degrees C in three, 12 and 14 dogs, respectively. No specimen collected from any dog with non-fungal nasal disease yielded fungal growth at room temperature or at 37 degrees C. CLINICAL SIGNIFICANCE: The diagnosis of canine SNA is more likely to be confirmed following culture of mucosal biopsies or fungal plaques than nasal secretions sampled blindly with swabs. Incubating cultures at 37 degrees C is more likely to provide a diagnostic outcome than when samples are cultured at room temperature. Fungal culture of nasal specimens has good specificity for the diagnosis of SNA in dogs. [less ▲]

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See detailConcentration of allergen-specific IgE in serum and bronchoalveolar lavage fluid in dogs with eosinophilic bronchopneumopathy and dogs with chronic bronchitis.
Peyron, Clémence ULg; Derer, Michal; Day, M. J. et al

in Proceedings of the 19th Annual Congress of the ECVIM-CA (2009)

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See detailClinical efficacy of intrasinusal administration of bifonazole cream through perendoscopically placed catheters alone or in combination with enilconazole irrigation in canine sinonasal aspergillosis.
Billen, Frédéric ULg; Guieu, Liz-Valérie; Bernaerts, Frédérique et al

in Proceedings of the 18th ECVIM-CA Congress (2008, September)

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See detailComparison of three serological tests for the diagnosis of canine sino-nasal aspergillosis.
Billen, Frédéric ULg; Peeters, Dominique ULg; Peters, Iain et al

in Proceedings of the 18th ECVIM-CA Congress (2008, September)

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See detailComparison of bronchoalveolar lavage cytospins and smears in dogs and cats.
Dehard, Sandrine ULg; Bernaerts, Frederique ULg; Peeters, Dominique ULg et al

in Journal of the American Animal Hospital Association (2008), 44(6), 285-94

Differences in the cytological interpretation of bronchoalveolar lavage fluid (BALF) after cytospin preparation (CP) or manual smearing of pelleted cells preparation (MSP) were investigated in client ... [more ▼]

Differences in the cytological interpretation of bronchoalveolar lavage fluid (BALF) after cytospin preparation (CP) or manual smearing of pelleted cells preparation (MSP) were investigated in client-owned dogs and cats with inflammatory or infectious lower respiratory disease. Bronchoalveolar lavage fluid from healthy cats was also examined. With MSP, cell lysis was more frequently observed, and cellular distribution was more heterogeneous throughout the slide. When samples from healthy and diseased animals were considered together, a significantly greater percentage of neutrophils was seen on CP than on MSP slides (P<0.002). Cytospin preparations were considered of better quality in all individual comparisons. Cytospin preparation is advised in the evaluation of BALF with low total cell count. When only MSPs are evaluated, clinicians should be aware that differential neutrophil counts may underestimate the counts found on CP slides. [less ▲]

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See detailFamilial glomerulopathy in seven French Mastiff dogs.
Lavoué, Rachel ULg; Day, MJ; Busoni, Valeria ULg et al

in Proceedings of the 18th Annual Congress of the ECVIM-CA (2008)

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See detailWhole Blood and Tissue Fungal DNA Quantification in the Diagnosis of Canine Sino-Nasal Aspergillosis
Peeters, Dominique ULg; Peters, I. R.; Helps, C. R. et al

in Veterinary Microbiology (2008), 128(1-2), 194-203

Various combinations of tests are used to confirm the diagnosis of canine sino-nasal aspergillosis (SNA) because false-positive and false-negative results can occur with each test. Therefore, the aim of ... [more ▼]

Various combinations of tests are used to confirm the diagnosis of canine sino-nasal aspergillosis (SNA) because false-positive and false-negative results can occur with each test. Therefore, the aim of this study was to evaluate whether detection of fungal DNA in blood and nasal tissue samples was of value in the clinical diagnosis of this disease. Four groups were included in the study (dogs with SNA, lymphoplasmacytic rhinitis or nasal neoplasia, and control animals). Real-time PCR assays detecting DNA from all Penicillium and Aspergillus species (PenAsp assay) or species-specific DNA from A. fumigatus, A. terreus, A. flavus and A. niger were applied to whole blood and nasal tissue samples. Results obtained by PCR were compared between the groups. Sensitivity, specificity, positive and negative predictive values (PPV and NPV) for fungal DNA detection were compared with those for alternative diagnostic procedures including histopathology, serology and fungal culture. Significantly more fungal DNA was detected by the PenAsp assay in tissue biopsies from dogs with SNA than in the three other groups. Sensitivity, specificity, PPV and NPV for this method were 1.00, 0.06, 0.32 and 1.00. A. fumigatus DNA was detected in seven tissue biopsies from dogs with SNA and in one biopsy from a dog with a nasal tumour. Sensitivity, specificity, PPV and NPV for this diagnostic test were 0.50, 0.97, 0.87 and 0.82. No significant difference was found between the groups with respect to the amount of DNA detected in blood by the PenAsp assay. Sensitivity, specificity, PPV and NPV for this method were 0.71, 0.24, 0.31 and 0.64. A. fumigatus DNA was detected in the blood of three dogs with SNA and sixteen dogs without SNA. Sensitivity, specificity, PPV and NPV for this diagnostic tool were 0.21, 0.45, 0.15 and 0.54. Detection of A. fumigatus DNA in nasal tissue had the highest specificity, PPV and NPV but sensitivity of this method was low. Detection of fungal DNA in whole blood was of no value in the diagnosis of SNA. [less ▲]

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See detailDetection of antigenic heterogeneity in feline coronavirus nucleocapsid in feline pyogranulomatous meningoencephalitis.
Poncelet, Luc; Coppens, Angélique; Peeters, Dominique ULg et al

in Veterinary Pathology (2008), 45(2)

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See detailLe point sur le diagnostic de l’aspergillose naso-sinusale chez le chien.
Billen, Frédéric ULg; Clercx, Cécile ULg; Peeters, Dominique ULg

in Annales de Médecine Vétérinaire (2008), 152(2)

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See detailPrimary ciliary dyskinesia in a family of Old English Sheepdogs
Billen, Frédéric ULg; Binst, Dominique; Geyskens, Valerie et al

in Proceedings of the 17th ECVIM-CA congress (2007, September)

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See detailWall stent placement to prevent coils migration during intrahepatic embolisation of porto-systemic shunt in a dog
Bolen, Géraldine ULg; De Busscher, Virginie; Cavrenne, Romain et al

Poster (2007, September)

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See detailDevelopment and application of multiple internal reference (housekeeper) gene assays for accurate normalisation of canine gene expression studies
Peters, I. R.; Peeters, Dominique ULg; Helps, C. R. et al

in Veterinary Immunology and Immunopathology (2007), 117(1-2), 55-66

Measurement of mRNA expression by real-time RT-PCR (QRT-PCR) has proven to be an important and powerful tool for the investigation of the pathogenesis of inflammatory and immune-mediated diseases in many ... [more ▼]

Measurement of mRNA expression by real-time RT-PCR (QRT-PCR) has proven to be an important and powerful tool for the investigation of the pathogenesis of inflammatory and immune-mediated diseases in many species. This methodology has proven particularly valuable in the dog, a species for which there are currently few specific antibodies for measurement of relevant proteins. Internal control (housekeeper) mRNAs are widely used for normalisation of QRT-PCR results. The validation and use of multiple internal control mRNAs for increased accuracy of normalisation has been described for humans and rodents. The aims of this study were to develop QRT-PCR assays for 11 potential internal control mRNAs in the dog (ACTB, B2M, G3PDH, HMBS, HPRT1, RPL 13A, RPL32, RPS 18, SDHA, TBP and YWAZ) and validate their use with bone marrow, colon, duodenum, heart, kidney, liver, lung, lymph node, skeletal muscle, pancreas, spleen and stomach from seven dogs. Endoscopic biopsies of the superficial duodenal mucosa were also obtained from nine dogs suffering from chronic gastro-oesophageal disease. The most stably expressed genes varied in the tissues examined. RPL13A and RPL32 (both components of the 60S ribosomal subunit) were the most stably expressed genes in the majority of the tissues examined, whereas ACTB and B2M were the least stable. Distinct internal control genes were shown to be most appropriate for use in full-thickness versus superficial mucosal biopsies of the duodenum. The results of this study indicate that there are no universal control genes for gene expression studies in canine tissues. It is important to use multiple internal control genes based upon a survey of potential control genes applied to representative samples from different disease groups, culture conditions and/or time points in an experimental study. (C) 2007 Elsevier B.V. All rights reserved. [less ▲]

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See detailChyloabdomen caused by a thrombus in the portal vein in a dog with immune-mediated hemolytic anemia and thrombocytopenia
Cavrenne, Romain ULg; Lavoué, Rachel; Peeters, Dominique ULg et al

in Proceedings of the first Belgium International Congress for Small Animal Veterinarians (Canifelis 2007) (2007, January)

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See detailVaccination in dogs and cats: between adverse reactions and duration of immunity
Peeters, Dominique ULg

in Proceedings of the first Belgium International Congress for Small Animal Veterinarians (Canifelis 2007) (2007, January)

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See detailHow to face failure to growth in dogs and cats?
Peeters, Dominique ULg

in Proceedings of the first Belgium International Congress for Small Animal Veterinarians (Canifelis 2007) (2007, January)

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See detailUpdate on Canine Sinonasal Aspergillosis
Peeters, Dominique ULg; Clercx, Cécile ULg

in Veterinary Clinics of North America. Small Animal Practice (2007)

Sinonasal aspergillosis is a frequent cause of nasal discharge that occurs in otherwise healthy, young to middle-aged dogs. A local immune dysfunction is suspected in affected animals, and the role of ... [more ▼]

Sinonasal aspergillosis is a frequent cause of nasal discharge that occurs in otherwise healthy, young to middle-aged dogs. A local immune dysfunction is suspected in affected animals, and the role of increased interleukin-10 mRNA expression in the nasal mucosa of affected dogs is currently under investigation. Despite recent advances in imaging techniques, the "gold standard" for diagnosing the disease is direct visualization of fungal plaques during endoscopy or observation of fungal elements on cytology or histopathologic examination. Treatment can be challenging; however, the use of topical enilconazole or clotrimazole through noninvasive techniques has increased the success of treatment and decreased the morbidity and duration of hospitalization. [less ▲]

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See detailCanine eosinophilic bronchopneumopathy
Clercx, Cécile ULg; Peeters, Dominique ULg

in Veterinary Clinics of North America. Small Animal Practice (2007)

Eosinophilic bronchopneumopathy (EBP) is a disease characterized by eosinophilic infiltration of the lung and bronchial mucosa, as demonstrated by examination of bronchoalveolar lavage fluid cytologic ... [more ▼]

Eosinophilic bronchopneumopathy (EBP) is a disease characterized by eosinophilic infiltration of the lung and bronchial mucosa, as demonstrated by examination of bronchoalveolar lavage fluid cytologic preparations or histologic examination of the bronchial mucosa. Although the precise cause of EBP is unknown, a hypersensitivity to aeroallergens is suspected. The diagnosis relies on typical history and clinical signs, demonstration of bronchopulmonary eosinophilia by cytology or histopathologic examination, and exclusion of known causes of lower airway eosinophilia. Most dogs display an excellent response to oral corticosteroid therapy; however, side effects of this treatment can be limiting. New therapeutic approaches are being studied, including the use of aerosol therapy, cyclosporine, or drugs interfering with T helper 2 immune response. [less ▲]

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See detailImmunohistochemical characterisation of the inflammatory infiltrate associated with canine nasal carcinoma.
Vanherberghen, Morgane ULg; Day, M. J.; Gabriel, Alexandra et al

in Proceedings of the 17th Annual Congress of the ECVIM-CA (2007)

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See detailDistinct tissue cytokine and chemokine mRNA expression in canine sino-nasal aspergillosis and idiopathic lymphoplasmacytic rhinitis
Peeters, Dominique ULg; Peters, I. R.; Helps, C. R. et al

in Veterinary Immunology and Immunopathology (2007), 117

Idiopathic lymphoplasmacytic rhinitis (LPR) and sino-nasal aspergillosis (SNA) are among the most common causes of nasal discharge in dogs. The pathogenesis of both diseases is poorly understood. Some ... [more ▼]

Idiopathic lymphoplasmacytic rhinitis (LPR) and sino-nasal aspergillosis (SNA) are among the most common causes of nasal discharge in dogs. The pathogenesis of both diseases is poorly understood. Some have proposed that LPR is a chronic inflammatory response to an inhaled irritant, pollutant or allergen, but others suggest that most cases of LPR constitute undiagnosed cases of SNA. Local immune dysfunction is thought to permit opportunist infection in canine SNA. This study investigates the nature of the local tissue immune response mounted in canine LPR and SNA in order to determine whether these diseases have similar or distinct pathogenesis. Quantitative reverse transcriptase polymerase chain reaction was carried out on RNA isolated from nasal biopsies from diseased and control dogs, using specific assays designed to amplify messenger RNA (mRNA), encoding a panel of cytokines and chemokines. SNA was associated with significantly increased expression of mRNA encoding interleukin (IL)-6, IL-8, IL-10, IL-12p19, IL-12p35, IL-12p40, IL-18, IFN-gamma, TNF-alpha, TGF-beta, eotaxin-2 and all four monocyte chemoattractant proteins (MCPs) relative to controls. LPR was associated with significantly increased expression of mRNA encoding IL-5, IL-8, IL-10, IL-12p19, IL12p40, IL-18, TNF-alpha, TGF-(3, MCP-2 and MCP-3 relative to controls. There was significantly more expression of mRNA encoding IL-6, IL-8, IL-10, IL-12p35, IL-12p40, IL-18, IFN-gamma, TNF-alpha, TGF-(3 and all MCPs, and significantly less expression of IL-5 in dogs with SNA than in dogs with LPR. Thus, the profile of cytokine and chemokine gene expression in the nasal mucosa is different in dogs with LPR when compared to dogs with SNA. A partial Th2 immune response appears to be mounted in the nasal mucosa of dogs with LPR, whereas the mucosal immune response in canine SNA is of the Th1 type. Increase in IL-10 and TGF-(3 transcripts in dogs with SNA is thought to be implicated in the failure to clear the Aspergillus infection. These results constitute the first evidence that the pathogenesis of canine LPR and SNA is distinct. (C) 2007 Elsevier B.V All rights reserved. [less ▲]

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See detailInfluence of age on bronchoscopical findings in healthy beagle dogs
Mercier, Elise ULg; Bolognin, Myriam; Delvaux, François ULg et al

in Proceedings of the 17th Annual Congress of the ECVIM-CA (2007)

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