References of "Peeters, Dominique"
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See detailDiagnosis of canine sino-nasal aspergillosis: is quantification of Aspergillus DNA a useful technique?
Peeters, Dominique ULg; Peters, I. R.; Helps et al

in Proceedings of the 24th VCRS meeting (2006)

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See detailCytokine and chemokine expression in canine sino-nasal aspergillosis and idiopathic lymphoplasmacytic rhinosinusitis.
Peeters, Dominique ULg; Peters, I. R.; Helps, C. et al

in Proceedings of the 16th Annual Congress of the ECVIM-CA (2006)

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See detailDistribution of leucocyte subsets in the canine pharyngeal tonsil
Billen, Frédéric ULg; Peeters, Dominique ULg; Dehard, Sandrine ULg et al

in Journal of Comparative Pathology (2006), 135(2-3, Aug-Oct), 63-73

This report describes the distribution and nature of lymphoid tissue in the nasopharyngeal mucosa of six puppies (mean age +/- SD, 0.3 +/- 0.25 years) and eight adult dogs (mean age +/- SD, 8.8 +/- 2.67 ... [more ▼]

This report describes the distribution and nature of lymphoid tissue in the nasopharyngeal mucosa of six puppies (mean age +/- SD, 0.3 +/- 0.25 years) and eight adult dogs (mean age +/- SD, 8.8 +/- 2.67 years) without respiratory disease. A non-encapsulated area of organized mucosa-associated lymphoid tissue was observed in the caudal part of the posterior wall of the nasopharynx, distal to the openings of the auditory tubes. This structure was consistent with the pharyngeal tonsil and was microscopically more extensive in puppies than in adult dogs. Histochemistry and immunohistochemistry were used to characterize and enumerate the leucocyte subsets in this part of the nasopharynx. Mast cells were found immediately beneath the respiratory epithelium but were also scattered in the glandular and muscular tissue. IgA(+) plasma cells outnumbered IgG(+) and IgM(+) plasma cells, especially in the glandular tissue. All classes of plasma cells were present in significantly greater numbers in adults than in puppies. MHC class II+ cells were mainly observed in areas containing diffuse and follicular aggregates of lymphoid cells. Both MHC class II+ cells and CD1c(+) cells with a dendritic morphology were predominantly found immediately beneath or within the epithelium, and cells expressing these markers were more abundant in puppies than in adult dogs. The anti-L1 marker labelled low numbers of cells with a neutrophilic morphology, which were significantly more abundant in puppies than in adult dogs. The majority of lymphoid cells were CD3(+) T lymphocytes and these were particularly abundant in areas containing aggregates of lymphold cells; CD4(+), CD8(+) and TCR alpha beta(+) cells had the same distribution as the CD3(+) cells. CD4(+) cells were more numerous than CD8(+) cells. The quantitative and qualitative data obtained will enable comparisons to be made with similar studies in dogs suffering from nasopharyngeal diseases, or when the local immune system needs to be investigated. (c) 2006 Elsevier Ltd. All rights reserved. [less ▲]

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See detailQuantification of mRNA encoding cytokines and chemokines in nasal biopsies from dogs with sino-nasal aspergillosis
Peeters, Dominique ULg; Peters, I. R.; Clercx, Cécile ULg et al

in Veterinary Microbiology (2006), 114(3-4), 318-326

Canine sino-nasal aspergillosis is usually caused by Aspergillus fumigatus and is similar to human chronic erosive non-invasive fungal sinusitis. The pathogenesis of the disease is poorly understood. We ... [more ▼]

Canine sino-nasal aspergillosis is usually caused by Aspergillus fumigatus and is similar to human chronic erosive non-invasive fungal sinusitis. The pathogenesis of the disease is poorly understood. We investigated the nature of the local immune response mounted in canine sino-nasal aspergillosis. Quantitative RT-PCR was carried out on RNA isolated from nasal biopsies from diseased and control dogs, using specific assays designed to amplify mRNA encoding a panel of cytokines and chemokines. Canine sino-nasal aspergillosis was associated with significantly increased expression of mRNA encoding MCP-1, -2, -3 and -4, IL-8, IL-10, EL-18 and TNF-alpha relative to controls (P < 0.01) but there was no difference between groups with respect to IL-4, IL-5, IL-6, IL-12, TGF-beta, and eotaxin-2 and -3. The up-regulation of proinflammatory cytokines and chemokines related to the influx of phagocytic cells might account for the localisation of this infection to the upper respiratory tract. The up-regulation of the expression of the immunomodulatory cytokine IL-10 in nasal tissue from affected dogs might be important in limiting the extent of local tissue destruction, but might also account for the fact that infected dogs are generally unable to clear this infection spontaneously. (c) 2005 Elsevier B.V. All rights reserved. [less ▲]

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See detailEffect of sampling method and culture conditions on the result of fungal culture in the diagnosis of canine sino-nasal aspergillosis.
Legarrérès, A.; Clercx, Cécile ULg; Mignon, Bernard ULg et al

in Proceedings of the 24th VCRS meeting (2006)

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See detailReal-time RT-PCR quantification of mRNA encoding cytokines, CC chemokines and CCR3 in bronchial biopsies from dogs with eosinophilic bronchopneurnopathy
Peeters, Dominique ULg; Peters, I. R.; Clercx, Cécile ULg et al

in Veterinary Immunology and Immunopathology (2006), 110(1-2), 65-77

Idiopathic canine eosinophilic bronchopneumopathy (EBP) is a disease characterized by eosinophilic infiltration of the pulmonary interstitium and bronchial mucosa, a cause for which has not yet been ... [more ▼]

Idiopathic canine eosinophilic bronchopneumopathy (EBP) is a disease characterized by eosinophilic infiltration of the pulmonary interstitium and bronchial mucosa, a cause for which has not yet been discovered. A recent study, examining the relative proportion of various lymphocyte cell subsets within bronchoalveolar lavage fluid from dogs with EBP, has shown a selective increase in CD4(+) T-cells and a selective decrease in CD8(+) T-cells, suggesting that a similar Th2 immune response might occur in EBP. The aim of the present study was to determine the profile of cytokine, chemokine and CC chemokine receptor 3 (CCR3) messenger RNA (mRNA) expression in bronchial tissue from dogs with EBP. Real-time RT-PCR assays were used for the quantification of mRNA encoding for a panel of cytokines, CC chemokines and CCR3 in perendoscopic bronchial biopsies from eight dogs with EBP and seven age-matched control dogs. Messenger RNA transcribed from the housekeeping gene glyceraldehyde-3 -phosphate dehydrogenase was used for normalisation of the threshold cycle in order to determine the relative copy numbers of the transcripts. No significant difference in the expression of any cytokine, MCP-1, -2, -4 and CCR3 was found between control and EBP dogs. The expression of transcript for MCP-3, eotaxin-2 and -3 was significantly greater in bronchial biopsies from dogs with EBP than in samples from control dogs while there was significantly less mRNA encoding RANTES in the mucosa of dogs with EBP. In conclusion, the cytokine mRNA expression profile in perendoscopic bronchial biopsies is similar in dogs with EBP and dogs without respiratory disease. Further studies on the quantification of mRNA encoding cytokines in isolated T lymphocytes from bronchoalveolar lavage fluid or bronchial biopsies are needed before any conclusion on the cytokine profile in canine EBP can be drawn. Eotaxin-2, -3 and MCP-3 appear to be implicated in the pathogenesis of the disease. (c) 2005 Elsevier B.V. All rights reserved. [less ▲]

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See detailSuspected drug-induced destructive cholangitis in a young dog.
Gabriel, Alexandra; van den Ingh, Ted; Clercx, Cécile ULg et al

in Journal of Small Animal Practice (2006), 47(6)

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See detailLaryngeal paralysis-polyneuropathy complex in young related Pyrenean Mountain dogs.
Gabriel, Alexandra; Poncelet, Luc; Van Ham, Luc et al

in Journal of Small Animal Practice (2006), 47(3)

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See detailHistochemical and immunohistochemical characterization of canine nasopharyngeal lymphoid tissue
Billen, Frédéric ULg; Peeters, Dominique ULg; Dehard, Sandrine ULg et al

in 15th ESVIM Meeting - Glasgow - Ecosse - Septembre 2005 (2005, September)

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See detailComparison of bronchoalveolar lavage cytospins ans smears in small animals
Dehard, Sandrine ULg; Bernaerts, Frederique ULg; Peeters, Dominique ULg et al

in 15th ESVIM Meeting - Glasgow - UK - 2005 (2005, September)

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See detailHistochemical and immunohistochemical characterisation of canine nasopharyngeal lymphoid tissue
Billen, Frédéric ULg; Peeters, Dominique ULg; Dehard, Sandrine ULg et al

in Proceedings of the 15th ECVIM-CA Congress (1-3/09/2005), Glasgow, Scotland (2005, September)

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See detailQuantification of mRNA encoding cytokines, chemokines and CCR3 in bronchial biopsies from dogs with eosinophilic bronchopneumopathy.
Peeters, Dominique ULg; Peters, I. R.; Clercx, Cécile ULg et al

in Proceedings of the 15th Annual Congress of the ECVIM-CA (2005)

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See detailQuantification of mRNA encoding cytokines and chemokines in nasal biopsies from dogs with sino-nasal aspergillosis.
Peeters, Dominique ULg; Peters, I. R.; Clercx, Cécile ULg et al

in Proceedings of the 15th Annual Congress of the ECVIM-CA (2005)

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See detailInhaled and systemic corticosteroids decrease Matrix Metalloproteinase-9 in bronchoalveolar lavage fluid of asthmatic cats
Leemans, Jérôme ULg; Kirschvink, Nathalie; Delvaux, François ULg et al

in In Proceedings:15th Congress European College of Veterinary Internal Medicine – companion animals (2005)

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See detailEffect of oral and inhaled corticosteroids on bronchial reactivity and airway inflammation in a feline model of asthma
Kirschvink, Nathalie; Leemans, Jérôme ULg; Delvaux, François ULg et al

in In proceedings: 15th Congress European College of Veterinary Internal Medicine – companion animals (2005)

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See detailAn immunohistochemical study of canine nasal aspergillosis
Peeters, Dominique ULg; Day, M. J.; Clercx, Cécile ULg

in Journal of Comparative Pathology (2005), 132(4), 283-288

In this study, histochemistry and immunohistochemistry were used to characterize the phenotype and distribution of leucocytes in the distal nasal mucosa of 15 dogs with nasal aspergillosis. The most ... [more ▼]

In this study, histochemistry and immunohistochemistry were used to characterize the phenotype and distribution of leucocytes in the distal nasal mucosa of 15 dogs with nasal aspergillosis. The most consistent histopathological finding was a severe, predominantly lymphoplasmacytic, inflammatory infiltration of the lamina propria. Fungal hyphae were not observed to invade the mucosa but were found at the mucosal surface and within material collected from the nasal cavity. The main immunohistochemical findings were (1) a predominance of IgG(+) plasma cells over IgA(+) and IgM(+) plasma cells, (2) significant numbers of macrophages and dendritic cells expressing MHC class 11 molecules, (3) macrophages and neutrophils expressing L1 antigen and (4) a mixture of CD4(+) and CD8(+) T cells. These findings are consistent with a dominant Th1-regulated cell-mediated immune response. The nature of the inflammatory infiltrate and the lack of invasiveness of the mucosa by the fungus, together with the clinical course of the disease and the apparent immunocompetence of the affected dogs, suggest that canine nasal aspergillosis resembles the chronic erosive non-invasive fungal sinusitis described in human patients. © 2004 Elsevier Ltd. All rights reserved. [less ▲]

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See detailReal-time RT-PCR quantification of mRNA encoding cytokines and chemokines in histologically normal canine nasal, bronchial and pulmonary tissue
Peeters, Dominique ULg; Peters, I. R.; Farnir, Frédéric ULg et al

in Veterinary Immunology and Immunopathology (2005), 104(3-4), 195-204

Cytokines and chemokines are likely to be involved in the pathogenesis of inflammatory diseases of the canine respiratory tract. The roles and relative amounts of these molecules have not yet been defined ... [more ▼]

Cytokines and chemokines are likely to be involved in the pathogenesis of inflammatory diseases of the canine respiratory tract. The roles and relative amounts of these molecules have not yet been defined in the respiratory mucosa of normal dogs or dogs with naturally acquired respiratory inflammation. In the present study, real-time reverse transcriptase polymerase chain reaction (RT-PCR) assays were employed to quantify messenger RNA (mRNA) encoding the chemokines monocyte chernotactic protein (MCP)-2, eotaxin-2 and eotaxin-3, and the cytokines interleukin-4 (IL-4), IL-5, IL-6, IL-10, IL-12p40, IL-18, interferon-gamma (IFN-gamma), tumour necrosis factor-alpha (TNF-alpha) and transforming growth factor-beta (TGF-beta) in normal nasal, bronchial and pulmonary tissues from puppies (n = 4) and from adult dogs (n = 7). There was no significant difference in the expression of any transcript between puppies and adult dogs at any of the anatomical sites examined. The expression of mRNA encoding eotaxin-2 and eotaxin-3 increased significantly with progression from the nasal mucosa to pulmonary parenchyma but expression of MCP-2 mRNA did not show this trend. At all levels of the respiratory mucosa, the most abundant transcripts were those encoding IL-18 and TGF-beta. Transcripts encoding IL-6, IL-10, IL-12 and TNF-alpha were approximately ten-fold less abundant, and IL-4, IL-5 and IFN-gamma were the least abundant templates. There was significantly different amount of mRNA encoding IL-5, IL- 18 and TNF-alpha between particular anatomical levels of the respiratory mucosa while the mRNA expression of the other cytokines was similar at all anatomical sites. The results of the present study will enable comparisons to be made with results obtained from similar samples obtained from dogs with nasal, bronchial or pulmonary diseases. (c) 2004 Elsevier B.V. All rights reserved. [less ▲]

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See detailDistribution of leucocyte subsets in the canine respiratory tract
Peeters, Dominique ULg; Day, M. J.; Farnir, Frédéric ULg et al

in Journal of Comparative Pathology (2005), 132(4), 261-272

Histochemistry and immunohistochemistry were used to characterize leucocyte subsets in the respiratory tract of 15 outbred dogs (five aged <6 months and 10 aged >1 year) that had no evidence of ... [more ▼]

Histochemistry and immunohistochemistry were used to characterize leucocyte subsets in the respiratory tract of 15 outbred dogs (five aged <6 months and 10 aged >1 year) that had no evidence of respiratory disease. No organized nose- or bronchus-associated lymphoid tissue was observed in any of the sections examined. IgA(+) plasma cells predominated in nasal mucosa and in all parts of the bronchial tree, with fewer IgG(+) and IgM(+) plasma cells. The numbers of IgA(+) and IgM(+) cells were significantly greater in the nasal mucosa than in any other part of the respiratory mucosa. There were significantly fewer IgA(+), IgG(+) and IgM(+) cells in all parts of the respiratory tract in the puppies than in the adults. The number and distribution of mast cells and cells expressing MHC class II, L1 or CD1c were recorded. Mast cells were mainly found in the subepithelial lamina propria of nasal and bronchial mucosa and in the alveolar interstitium, and cells expressing IgE had a similar distribution. Mast cells were also present within muscle layers of the bronchial tree. The numbers of mast cells and MHC class II(+) cells were significantly greater in the nasal mucosa than in any other part of the respiratory mucosa. In the nose, carina and primary and secondary bronchus, there were significantly more mast cells and MHC class II(+) cells in puppies than in adult dogs, whereas the numbers of L1(+) cells and CD1c(+) cells in most sites were significantly greater in older dogs. There were significantly more CD3(+) and CD8(+) cells in the nasal mucosa than in any part of the bronchial mucosa. In most parts of the respiratory mucosa, CD4(+), CD8(+) and TCR alphabeta(+) cells were present in significantly greater numbers in adults than in puppies. All parts of the respiratory tract had similar numbers of mucosal CD4(+) and CD8(+) T lymphocytes. TCR gammadelta(+) cells were absent or sparse in all samples. These data, obtained from dogs without respiratory disease, will enable comparisons to be made with dogs suffering from infectious or inflammatory nasal, bronchial and pulmonary diseases. [less ▲]

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See detailDistribution of leucocyte subsets in bronchial mucosa from dogs with eosinophilic bronchopneumopathy
Peeters, Dominique ULg; Day, M. J.; Clercx, Cécile ULg

in Journal of Comparative Pathology (2005), 133(2-3, Aug-Oct), 128-135

Immunohistochemistry was used to characterize the distribution of leucocyte subsets in the bronchial mucosa of 11 dogs with idiopathic eosinophilic bronchopneumopathy (EBP). Formalin-fixed tissues from ... [more ▼]

Immunohistochemistry was used to characterize the distribution of leucocyte subsets in the bronchial mucosa of 11 dogs with idiopathic eosinophilic bronchopneumopathy (EBP). Formalin-fixed tissues from all dogs were included in the study, but frozen tissue from only one dog was available. MHC class II(+) cells were found in moderate numbers in the lamina propria (LP). These cells were morphologically either dendritic-like cells or macrophages, but many macrophages did not express MHC class II. Such molecules were expressed by occasional fibroblasts. L1(+) cells, which formed a relatively small component of the LP inflammatory infiltrate, were morphologically either macrophages or polymorphonuclear cells (probably eosinophils). IgA(+) plasma cells were found in varying numbers in the LP, mostly in association with glandular tissue. IgG(+) plasma cells were less common, and IgM(+) plasma cells were present in low numbers. Many CD3(+) cells were present in the LP. In the single case from which frozen tissue was available, most of the lymphocytes were labelled with CD4 marker, while smaller numbers were CD8(+) T cells. Most of the lymphocytes in this case were positively labelled with T-cell receptor (TCR)-alphabeta marker. TCR-gammadelta(+) cells, although less common, were present in significant numbers throughout the LP. CDlc(+) dendritic cells were numerous in the epithelium and in the LP, immediately beneath the basement membrane. These findings, which were similar to those described in human asthma, are suggestive of a Th2 dominant immune response in canine EBP. As in human asthma, this provides a possible basis for new forms of treatment in canine EBP. [less ▲]

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