Regulation of the expression of the c-erbB2 gene, an adenocarcinoma specific oncogene, in cultured human mammary cancer cell lines : a molecular approach.

Conference (1991, September)

Half-life of the c-erbB2 mRNA synthesized in human mammary adenocarcinoma cell lines.

Poster (1991, June)

Cloning and sequencing the upstream regulatory regions of the c-erbB2 gene.

in Archives Internationales de Physiologie, de Biochimie et de Biophysique (1990), 98(B40),

Towards the design and the synthesis of an artificial alpha/beta protein.

Conference (1988, February)

A comparison of bovine growth hormone expression directed by bGH genomic or intronless DNA in transiently transfected eukaryotic cells.

in Gene. (1987), 57

Two recombinant DNA plasmids were constructed with identical transcriptional and translational regulatory elements controlling expression of the bovine growth hormone (bGH) gene or the bGH gene lacking ... [more ▼]

Two recombinant DNA plasmids were constructed with identical transcriptional and translational regulatory elements controlling expression of the bovine growth hormone (bGH) gene or the bGH gene lacking introns. Transient expression of these plasmids in cultured eukaryotic cells, monitored by assaying secretion of bGH into the culture medium, was employed to examine the relative importance of introns in the expression of this gene. The bGH gene lacking introns is expressed more efficiently than the bGH gene in avian and mammalian cells. [less ▲]

Location of transcriptional regulatory sequences within the prolactin family genes.

in Neuroendocrine Perspectives. (1986)

Location of transcriptional and regulatory sequences within the prolactin family genes

in Müller, E. E.; McLeod, R. M. (Eds.) Neuroendocrine Perspectives (1986)

Growth hormone gene expression in eukaryotic cells directed by the rous sarcoma virus long terminal repeat or cytomegalovirus immediate early promoter-regulator.

Poster (1985, April)

Genetic regulation of hepatic steroid 16 alpha-hydroxylase activities in inbred strains of mice.

in Endocrinology (1984), 115

Steroid 16 alpha-hydroxylase activities and properties were studied in C57Bl/6J, 129/J, AKR/R, DBA/2J, C3H/I, and BALB/c mouse liver using four different substrates. The highest enzymatic activities were ... [more ▼]

Steroid 16 alpha-hydroxylase activities and properties were studied in C57Bl/6J, 129/J, AKR/R, DBA/2J, C3H/I, and BALB/c mouse liver using four different substrates. The highest enzymatic activities were measured in the female mice, with the exception of the 129/J females. As in the rat liver, the sexual differentiation of the steroid 16 alpha-hydroxylation observed in adult male and female mice took place at puberty. In the adult mouse liver, two steroid 16 alpha-hydroxylase activities (forms I and II) could be differentiated on the basis of their relative affinities for the various steroid substrates and their relative proportions in male and female mouse livers. In the immature mouse liver, no sexual differences could be detected, and the mice of both sexes presented phenotypes identical to those of the adult female. The adult 129/J females appeared genetically deficient with respect to the form I of the steroid 16 alpha-hydroxylase and presented a phenotype identical to that of the adult male mice of the various strains tested. Differences in hydroxylase activities between the C57Bl/6J and 129/J strains were investigated using standard genetic breeding protocols. Steroid 16 alpha-hydroxylase seemed to be inherited additively in the liver of the female mice obtained by crossing the C57Bl/6J male and the 129/J female or the 129/J male and the C57Bl/6J female. In the male mice, regardless of genotype, the observed phenotype was always identical to the two male parental types. Both hormonal and genetic regulations were responsible for the different phenotypes occurring in adult male and female C57Bl/6J and 129/J mouse livers. [less ▲]

Genetic regulation of hepatic steroid 16alpha-hydroxylase activities in inbred strains of mice.

Poster (1982, September)