References of "PINCEMAIL, Joël"
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See detailInvestigation of blood oxidant/antioxidant markers in healthy competition horses of different breeds
Kirschvink, Nathalie; de Moffarts, Brieuc; Farnir, Frédéric ULg et al

in Equine Veterinary Journal. Supplement (2006), 36

REASONS FOR PERFORMING STUDY: There is increasing evidence that the equine athlete is exposed to exercise-induced changes of its oxidant/antioxidant balance and antioxidant supplementation is frequently ... [more ▼]

REASONS FOR PERFORMING STUDY: There is increasing evidence that the equine athlete is exposed to exercise-induced changes of its oxidant/antioxidant balance and antioxidant supplementation is frequently recommended. However, it is unknown whether there is a specific need for antioxidants according to performance, breed, gender or age. OBJECTIVES: To assess whether breed-, gender- and age-related differences of blood oxidant/antioxidant markers occur in competition horses. METHODS: Healthy horses (n = 493) underwent oxidant/ antioxidant blood marker determination. Vitamin E, lipophilic antioxidant capacity (ACL), ascorbic acid (AA), glutathione (GSH, GSSG), gluthione peroxidase (GPx), superoxide dismutase (SOD), selenium (Se), copper (Cu), zinc (Zn), lipid peroxides (Pool), oxidised proteins (Protox) were determined, as well as magnesium (Mg), creatine phosphokinase (CPK), lactate dehydrogenase (LDH), packed cell volume (PCV) and haemoglobin (Hb). A mixed linear model assessed the effect of breed, gender and age category. P<0.05 was considered significant. RESULTS: Thoroughbreds showed the highest values of vitamin E, ACL, GPx, PCV and Hb, whilst standardbreds had the highest values of AA and LDH. Jumping horses had the highest Protox values. Females had significantly higher SOD values, whereas most of the other markers were higher in stallions and geldings. Horses age 2-6 years had higher AA, SOD and LDH values than horses age >6 years. Correlation analyses were positive and significant between vitamin E and GPx, VitE and ACL, Se and GPx, Cu and Pool and negative between Pool and vitamin E, Pool and ACL, Protox and GPx, Protox and vitamin E. CONCLUSIONS: Blood oxidant/ antioxidant status of horses is influenced by breed, gender and age. The correlation analyses suggest synergistic relations between GPx, vitamin E and Se and an antagonistic relation between Protox-GPx, Protox-vitamin E, and Pool-vitamin E. POTENTIAL RELEVANCE: The results of this investigation provide definition of the specific need for antioxidants and vitamins in competition horses. [less ▲]

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See detailInvestigation of blood oxidant/antioxidant markers in healthy competition horses : effect of discipline and gender.
Kirschvink, N.; De Moffarts, Brieuc; Farnir, Frédéric ULg et al

in 7th International Conference on Equine Exercise Physiology (2006)

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See detailImpact physiologique et pathologique du stress oxydant chez le cheval
De Moffarts, Brieuc; Kirschvink, Nathalie; Pincemail, Joël ULg et al

in Annales de Médecine Vétérinaire (2005), 149(1), 1-9

Oxidative stress has become of increasing interest in research and in equine and human medicine. If the pro-oxidant burden overwhelms the endogenous antioxidant defence of the organism, the arising ... [more ▼]

Oxidative stress has become of increasing interest in research and in equine and human medicine. If the pro-oxidant burden overwhelms the endogenous antioxidant defence of the organism, the arising imbalance between pro- and antioxidants is defined as oxidative stress. Different pathways might increase the generation of reactive oxygen species ( ROS) and results in oxidative stress. In physiological conditions, like during moderated exercise, the balance between ROS production and antioxidants allows to maintain an optimal organic function. In the light of the potentially deleterious role of excessive ROS production and the evidence that exercise-induced oxidative stress occurs in horses, the assessment of the antioxidant status should be considered in sport horses. Recent researches indicate that oxidative stress may play a role in physiopathology of several pathological syndromes in horses. Therefore, with classical treatment, the controlled administration of antioxidants appears interesting for the modulation of these processes, as well as in sport horses during intense exercise period. [less ▲]

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See detailEffect of oral antioxidant supplementation on blood antioxidant status in trained thoroughbred horses
De Moffarts, Brieuc; Kirschvink, Nathalie; Art, Tatiana ULg et al

in Veterinary Journal (2005), 169(1), 65-74

The oxidant/antioxidant equilibrium of trained thoroughbred horses (n = 40) was assessed on three occasions during a period of three months under field conditions by blood antioxidant markers analysis, i ... [more ▼]

The oxidant/antioxidant equilibrium of trained thoroughbred horses (n = 40) was assessed on three occasions during a period of three months under field conditions by blood antioxidant markers analysis, i.e. plasma ascorbic acid (AA), plasma antioxidant capacity of water-soluble components (ACW), whole blood (GSH) and oxidised (GSSG) glutathione, plasma alpha-tocopherol and beta-carotene, plasma antioxidant capacity of lipid-soluble components (ACL), red blood cell superoxide dismutase (SOD) and glutathione-peroxidase activity (GPx) and plasma trace-elements, i.e. selenium (Se), copper (Cu), zinc (Zn). A control group of ten horses receiving a placebo and an antioxidant group of 30 horses orally supplemented with an antioxidant mixture were randomly formed. An antioxidant imbalance was observed after three months in the control group, reflected by a significant decrease in GSH, SOD, GPx, Se (P < 0.05) and a significant increase in GSSG (P < 0.05). The antioxidant supplement prevented GPx and Se decrease and significantly increased ACW, alpha-tocopherol, beta-carotene and ACL (P < 0.05). Significant sex- or age-related differences were found for AA, ACW, alpha-tocopherol, SOD, GPx and Se, and there were significant correlations between ACW-AA, ACL-alpha-tocopherol, GPx-Se, CPK-Se, CPK-alpha-tocopherol and CPK-Cu. This field study has shown that trained thoroughbred horses undergo significant changes of several blood antioxidant markers and that oral antioxidant supplementation might partially counterbalance these changes by improving the hydrophilic, lipophilic and enzymatic antioxidant blood capacity. (C) 2004 Elsevier Ltd. All rights reserved. [less ▲]

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See detailEffect of an oral antioxidant supplementation enriched in (n-3) fatty acids on erythrocyte membrane fluidity in horses.
De Moffarts, Brieuc; Portier, K.; Kirschvink, Nathalie et al

in Proceedings: Congrès de médecine et de chirurgie équine, Genève 2005 (2005)

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See detailAssessment of the oxidant-antioxidant blood balance in a field exercise test in Standardbred and eventing horses
De Moffarts, Brieuc; Kirschvink, Nathalie; van Erck, Emmanuelle et al

in Equine & Comparative Exercise Physiology (2005), 2(4), 253-261

The aim of this study was to determüie which oxidant-antioxidant blood markers are of interest for a field exercise test (ET) performed on a racetrack. Healthy Standardbred herses (S: n = 12) and healthy ... [more ▼]

The aim of this study was to determüie which oxidant-antioxidant blood markers are of interest for a field exercise test (ET) performed on a racetrack. Healthy Standardbred herses (S: n = 12) and healthy eventing horses (E: n = 12) were investigated. Exercice was monitored by measuring velocity (V), heart rate (HR), and plasma lactate (LA). Whilst maximal LA did sot Biffer (11.8 ± 0.88 mmoll-1), maximal V (S: 12.3 ± 0.17ms-1 versus E: 11.1 ±0.24ms-1, P < 0,05) and final HR (S: 222 ±1 versus E: 203 ± 8 beats min-1, P < 0.03) were significantly différent between groups. Venous was collected at rest (R) prior to ET and The following oxidant-antioxidant markers were detennitted: uric acid (UA), ascorbic acid (AA), alpha-tocopherol (Vit E). vitamin A (Vit A), superoxide cüsmutase (SOB), glutaYhione peroxidase (GPx), glutathione (reduced: GSH and oxidized: GSS(r), glutathione redox ratio (GRR), copper (Cu), zinc (Zn) and sélénium (Se), uxidized proteins (Protox), lipid prrosides (Pool), antioxidant capacity of water-soluble comporteras (ACW) and antioxidant capacity of lipid-soluble components (ACW). The following markers were further determined 15 min (E15) after the ET:UA, ACW; AA, GSH, Proton, Pool, ACL. Standardbreds had significantly higher concentrations of ACW, GSH, ACL and Protox, whilst Se, Zn and SOI) were significantly lover than in eventing horses. Exercice induced a significant increase in ACW and UA. GSH decreaced in eventing horses and Pool significantly decreased in both horse groups. This study describes a field ET of high intensity for Standardbred and eventing horses, which could be performed by all animals tested. By sampling blond al rest and at E15, changes of thé hydrophilic antioxidant defence were partially assessed, whereas no interpretable changes of the lipophilic antioxidants and of oxidation markers (Protox, Pool) could be detected [less ▲]

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See detailOxidative stress in the liver and the brain of rats in fulminant hepatic failure
Detry, Olivier ULg; Gaspar, Yves; Cheramy-Bien, Jean-Paul ULg et al

in Transplantation Proceedings (2005), 37(6, Jul-Aug), 2883-2885

The etiological mechanisms of brain edema in fulminant hepatic failure are incompletely understood. In a surgical model of fulminant hepatic failure in the rat, we tested whether oxidative stress may be ... [more ▼]

The etiological mechanisms of brain edema in fulminant hepatic failure are incompletely understood. In a surgical model of fulminant hepatic failure in the rat, we tested whether oxidative stress may be involved in the early steps of brain edema. Moreover, we took advantage of this model to determine if oxidative stress may be involved in the hepatocyte dysfunction observed in the setting of fulminant hepatic failure. Oxidative stress was evaluated by measurement of tissue ascorbic acid in the brain and liver of rats at 6 hours after induction of fulminant hepatic failure versus in control or partially hepatectomized rats. After 6 hours, the level of ascorbic acid was not different in the brain tissue of the various groups, indicating no oxidative stress. The liver showed a significant decrease in ascorbic acid levels, both in ischemic and nonischemic liver tissue, suggesting that oxidative stress might be involved in the failure of liver regeneration in fulminant hepatic failure. In this rat model no oxidative stress was demonstrated in the brain during the early phase of fulminant liver failure. [less ▲]

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See detailGlutathione system deficiency in racehorses
De Moffarts, Brieuc; Kirschvink, N.; Art, Tatiana ULg et al

in Handbook of the 43rd Congress of the British Equine Veterinary Association (2004)

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See detailDéficience du système glutathion chez le cheval de course
De Moffarts, Brieuc; Kirschvink, N.; Art, Tatiana ULg et al

in 30ème Journée de la Recherche Equine, Les Haras Nationaux (2004)

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See detailEffet de l’entraînement et de l’intensité de l’exercice sur les marqueurs sanguins du stress oxydant chez le trotteur
De Moffarts, Brieuc; Kirschvink, Nathalie; Art, Tatiana ULg et al

in Proceedings: Congrès de l'Association Vétérinaire Equine Française (AVEF) (2004)

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See detailEffect of exercise on blood glutathione in healthy race horses
De Moffarts, Brieuc; Kirschvink, Nathalie; Art, Tatiana ULg et al

in Pflügers Archiv : European Journal of Physiology (2004), 447

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See detailImpact of training and exercise intensity on blood antioxidant markers in healthy Standardbred horses
De Moffarts, Brieuc; Kirschvink, Nathalie; Art, Tatiana ULg et al

in Equine & Comparative Exercise Physiology (2004), 1(3), 211-220

This study investigated the effect of training and exercise intensity on blood antioxidant markers in six healthy Standardbred horses. Markers studied were uric acid (UA), ascorbic acid (AA), α-tocopherol ... [more ▼]

This study investigated the effect of training and exercise intensity on blood antioxidant markers in six healthy Standardbred horses. Markers studied were uric acid (UA), ascorbic acid (AA), α-tocopherol, vitamin A (Vit A), superoxide dismutase (SOD), glutathione peroxidase (GPx), glutathione – reduced (GSH) and oxidized (GSSG), glutathione redox ratio (GRR), copper (Cu), zinc (Zn) and selenium (Se). The horses performed a standardized exercise test (SET) when they were untrained (T0), after 4 weeks of light training (T4) and after 8 weeks of interval training (T12). Forty-eight hours after SET T4 and SET T12, a SET with run up to fatigue (TTF4 and TTF12) was performed. Maximal oxygen consumption (V˙O2max) was determined within 3–5 days after TTF4 and TTF12. At each test (SET T(i) and TTF(i)), venous blood was sampled at rest (R), peak-exercise (Emax), 15 (E15) and 60 (E60) min after the test. UA, AA and GRR were increased significantly by the exercise, whereas GSH and Vit A were decreased significantly (P<0.05). Training-related increases (T0 vs. T4 vs. T12) were observed for UA, SOD, GPx and Se, whereas GSH, α-tocopherol and Zn decreased (P<0.05). Exercise intensity (TTF(i) vs. T(i)) increased UA and AA significantly and decreased GSH significantly (P<0.05). A significant correlation between ΔSOD and ΔV˙O2max (r=0.86, p<0.05) determined at SET T4 and SET T12 was found. Taken together, these results indicate that training and exercise intensity significantly influence blood antioxidant markers in healthy Standardbred horses [less ▲]

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See detailEffet d’un complément antioxydant sur les marqueurs sanguins du stress oxydant chez des galopeurs entraînés
De Moffarts, Brieuc; Kirschvink, N.; Art, Tatiana ULg et al

in Congrès de l'Association Vétérinaire Equine Française (AVEF) (2003)

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See detailIntracellular free iron content of rat liver tissue after cold ischemia
Pincemail, Joël ULg; Sergent, O.; Detry, Olivier ULg et al

in Transplantation Proceedings (2002), 34(3), 759-761

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See detailEffect of perioperative infusion of antioxidants on neutrophil activation during liver transplantation in humans
Biasi, F.; Poli, G.; Salizzoni, M. et al

in Transplantation Proceedings (2002), 34(3), 755-758

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See detailLevels of inflammatory markers in the blood processed by autotransfusion devices during cardiac surgery associated with cardiopulmonary bypass circuit
Amand, T.; Pincemail, Joël ULg; Blaffart, Francine ULg et al

in Perfusion (2002), 17(2), 117-123

Intraoperative blood salvage devices allowing a reinfusion of red blood cells (RBCs) after processing of shed blood and stagnant blood in the mediastinal cavity are more and more used to reduce homologous ... [more ▼]

Intraoperative blood salvage devices allowing a reinfusion of red blood cells (RBCs) after processing of shed blood and stagnant blood in the mediastinal cavity are more and more used to reduce homologous blood requirements in cardiac surgery with cardiopulmonary bypass (CPB). As the proinflammatory activity of the shed blood also contributes to morbidity during CPB, we conducted a prospective study in order to examine the quality of autologous blood before and after processing with five different devices [BRAT2, Sequestra, Compact Advanced, Cell Saver 5 (CS5), Continuous Autologous Transfusion System (CATS)]. All systems resulted in an excellent haemoconcentration, ranging from 53.7% (Compact) to 68.9% (CATS). The concentrations and elimination rates of several inflammatory markers [IL-1beta, IL-2, IL-8, TNFalpha, myeloperoxidase (MPO), elastase] were examined. Except for the Sequestra, an important increase in concentration of IL-1beta (between 30% and 220%) has been observed after processing with each device. In contrast, the attenuation rate of IL-6 and TNFalpha (95%) was optimal for all investigated blood salvages systems. Regarding IL-8, only the CATS and CS5 systems were able to attenuate this biological parameter with an excellent efficacy. The rate of attenuation in MPO and elastase, as markers of leukocyte activation, was higher than 80% for all devices. In conclusion, the different RBC washing systems tested in this study resulted in a significant attenuation of the inflammatory response. Increased levels of IL-1beta after processing remained, however, unclear. According to the type of protocol, based on inlet haematocrit, fill and wash speeds, and wash volumes, small variations in reducing the inflammatory response have been observed from one device to another. [less ▲]

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See detailS-acetyl-glutathione selectively induces apoptosis in human lymphoma cells through a GSH-independent mechanism
Locigno, Roberto; Pincemail, Joël ULg; Henno, Audrey et al

in International Journal of Oncology (2002), 20(1), 69-75

Reduced apoptosis is associated to cancer development. Agents able to restore the programmed cell death responsiveness of cancer cells are foreseen as potential effective cancer therapies. In this study ... [more ▼]

Reduced apoptosis is associated to cancer development. Agents able to restore the programmed cell death responsiveness of cancer cells are foreseen as potential effective cancer therapies. In this study, we report that a glutathione-S-derivative, S-acetyl-glutathione (Sag), induces significant apoptosis in three human lymphoma cell lines, including Daudi, Raji and Jurkat cells while it had no or little effect on either Hut-78 lymphoma cells or the normal BT lymphocytes. We used Annexin-V FACS analysis and DNA laddering to demonstrate that Sag activated apoptosis in the three sensitive cell lines in a dose- and time-dependent-fashion. Using mercury orange staining and FACS analysis, we showed that Sag generated an intracellular GSH depletion in Daudi, Raji and Jurkat cells but not in Hut-78 or the normal BT cells. These data provide direct evidence that Sag specifically activates programmed cell death in lymphoma cells through, at least in part, a depletion of intracellular GSH rather than an increase, as previously suggested. Because of its selective effect on cancer cells, Sag appears as a promising new lymphoma cell apoptosis inducer with potential clinical value for lymphoma patients. [less ▲]

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See detailOxidative stress status in top soccer players
Pincemail, Joël ULg; Bonjean, K.; Cayeux, C. et al

in Free Radical Biology & Medicine (2002), 33(Suppl. 1), 249-249

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