Un semis ponctue d'apparition rapide sur la peau d'un adolescent.
CAUCANAS, Marie ; FRANCHIMONT, Claudine ; PIERARD, Gérald
in Revue Médicale de Liège (2012), 67(1), 35-7
In some instances, a dermatosis exhibits a rapid and focal surge of seemingly similar tiny lesions. We report a few typical exemples occurring in adolescents.Detailed reference viewed: 6 (0 ULg)
Tracking and treating malignant melanoma metastases.
PIERARD, Gérald ; ; QUATRESOOZ, Pascale
in Dermatology Research and Practice (2012), 2012(art.173491), 1-2Detailed reference viewed: 17 (7 ULg)
Cell proliferation in malignant melanoma: relationship with neoplastic progression.
in ISRN Dermatology (2012), 2012(art.828146), 1-12Detailed reference viewed: 13 (3 ULg)
Télédermatopathologie et dermatopathologie virtuelle en temps réel.
REGINSTER, Marie-Annick ; Pierard, Sébastien ; PIERARD, Gérald et al
in Dermatologie Actualité (2012), 129Detailed reference viewed: 25 (3 ULg)
Pemetrexed-induced pityriasis lichenoides-like dermatitis.
SABATIELLO, Murielle ; ; et al
in Journal of the European Academy of Dermatology & Venereology (2012), 26(1), 127-9Detailed reference viewed: 32 (2 ULg)
Bioimpact of EGFR antagonists on the pilosebaceous follicles.
PIERARD, Gérald ; FRANCHIMONT, Claudine ;
in European Journal of Dermatology (2012), 22(1), 54-7
Cancer patients under targeted chemotherapy to the epidermal growth factor receptor (EGFR) frequently suffer from unusual skin adverse events. In the past, these changes were globally qualified as a rash ... [more ▼]
Cancer patients under targeted chemotherapy to the epidermal growth factor receptor (EGFR) frequently suffer from unusual skin adverse events. In the past, these changes were globally qualified as a rash. Our aim was to assess objectively by non invasive bioinstrumentation some early structural and functional skin changes associated with EGFR inhibitor treatment. A series of 27 cancer patients aged 58-66 years were assessed using two ultraviolet light emitting CCD cameras, Visioscan((R)) and Visiopor((R)). Assessments were performed on the foreheads at inclusion and therefore at weekly intervals for 2 months at most. No topical treatment was applied during the assessment period. The Visioscan((R)) camera revealed specular light reflectance at the site of follicular plugging. The interfollicular stratum corneum showed occasional focal hyperkeratosis. These features increased in severity with the EGFR inhibitor treatment, indicating follicular involvement as an early adverse event of the therapy. The follicular fluorescence revealed by the Visiopor((R)) camera remained unchanged over the treatment period. The present findings suggest an EGFR inhibitor-induced kerosis (follicular hyperkeratosis) possibly responsible for acneiform reactions. [less ▲]Detailed reference viewed: 18 (0 ULg)
Patterns of aluminum hydroxychloride deposition onto the skin.
; ; PIERARD, Gérald
in Skin Research & Technology (2012), 18(1), 64-9
BACKGROUND: Aluminum hydroxychloride (AlCl(3) ) is an antiperspirant. AIM: To revisit the AlCl(3) deposition in vivo and in vitro on glass slides and stratum corneum (SC) harvested by cyanoacrylate skin ... [more ▼]
BACKGROUND: Aluminum hydroxychloride (AlCl(3) ) is an antiperspirant. AIM: To revisit the AlCl(3) deposition in vivo and in vitro on glass slides and stratum corneum (SC) harvested by cyanoacrylate skin surface strippings (CSSS). METHODS: Transepidermal water loss (TEWL) was assessed following application of 5% AlCl(3) on the forearms. The AlCl(3) -coated skin, glass slides and CSSS were observed using two ultraviolet light-emitting CCD cameras in order to record changes in specular reflectance related to AlCl(3) deposition. In addition, the corneoxenometry bioassay was performed in order to predict AlCl(3) irritation. RESULTS: AlCl(3) deposited on glass slides looked as linear threads and rings of similar sizes. AlCl(3) deposits on skin were almost restricted inside the microrelief lines and as annular deposits at their crossings where acrosyringia are opening. After daily AlCl(3) applications, deposits extended on the CSSS plateaus. At rest in absence of sweating, TEWL was decreased following AlCl(3) applications. During physical exercise, the TEWL increase was limited on the AlCl(3) areas. CSSS appeared unreactive to AlCl(3) at the corneoxenometry bioassay. CONCLUSION: The similar aspect of AlCl(3) deposits on human SC and on glass slides suggested a physical property of AlCl(3) . Repetitive applications of AlCl(3) increased both the deposit area and the barrier function. [less ▲]Detailed reference viewed: 21 (1 ULg)
Ipilimumab, a promising immunotherapy with increased overall survival in metastatic melanoma?
PIERARD, Gérald ; ;
in Dermatology Research and Practice (2012), 2012
Malignant melanoma (MM) is one of the most aggressive skin cancer. The therapeutic options remain limited for advanced MM, and those directed to the neoplastic cells have not brought major survival ... [more ▼]
Malignant melanoma (MM) is one of the most aggressive skin cancer. The therapeutic options remain limited for advanced MM, and those directed to the neoplastic cells have not brought major survival advantage so far. Immunotherapy is another targeted option. Ipilimumab, a monoclonal antibody directed to CTLA-4 present on cytotoxic T cells boosts immunity, particularly its anti-MM activity. Under treatment, the overall survival of patients with MM metastases is moderately but significantly increased. The immuno-related adverse effects may be severe and life threatening. [less ▲]Detailed reference viewed: 12 (0 ULg)
The skin ivory spot. A possible indicator for skinfield photocarcinogenesis in recreational sunbed addicts.
QUATRESOOZ, Pascale ; FRANCHIMONT, Claudine ; PIERARD, Gérald
in International Journal of Environmental Research and Public Health (2012), 9Detailed reference viewed: 14 (1 ULg)
Smouldering malignant melanoma and metastatic dormancy: an update and review.
PIERARD, Gérald ; PIERARD-FRANCHIMONT, Claudine ; REGINSTER, Marie-Annick et al
in Dermatology Research and Practice (2012), 2012
The fund of knowledge regarding the versatility of presentation of MM metastases is still quite incomplete. The recent literature pertaining to the current understanding of the mechanisms underlying two ... [more ▼]
The fund of knowledge regarding the versatility of presentation of MM metastases is still quite incomplete. The recent literature pertaining to the current understanding of the mechanisms underlying two special features of MM metastasis is reviewed. On the one hand, a long disease-free interval (MM dormancy) may occur before the surge of overt metastases. On the other hand, the so-called MM smouldering phenomenon refers to the condition where regional metastases wax and wane for long periods of time on restricted skin regions. It is important to emphasize that local micrometastases often predict sentinel lymph node involvement but may not reflect progression of the primary MM to full-blown visceral metastatic competence. It is likely that a combination of factors impacts the versatile MM metastasic progression. Among the main factors, one has to mention the phenotypic heterogeneity and variability in the phenotype of MM cells, the presence of MM stem cells and MM cells engaged in an amplification proliferation pool, as well as the host immune response, and possibly the induction of a particular stromal structure and vascularity. [less ▲]Detailed reference viewed: 18 (4 ULg)
Molecular dermatopathology in malignant melanoma.
REGINSTER, Marie-Annick ; PIERARD-FRANCHIMONT, Claudine ; PIERARD, Gérald et al
in Dermatology Research and Practice (2012), 2012(684032), 1-6
At present, immunohistochemistry is taken for granted in the establishment of malignant melanoma (MM) diagnosis. In recent years, molecular diagnosis in dermatopathology has benefited from a vast array of ... [more ▼]
At present, immunohistochemistry is taken for granted in the establishment of malignant melanoma (MM) diagnosis. In recent years, molecular diagnosis in dermatopathology has benefited from a vast array of advances in the fields of genomics and proteomics. Sensitive techniques are available for detecting specific DNA and RNA sequences by molecular hybridization. This paper intends to update methods of molecular cytogenetics available as diagnostic adjuncts in the field of MM. Cytogenetics has highlighted the pathogenesis of atypical melanocytic neoplasms with emphasis on the activation of the mitogen-activated protein kinase (MAPK) signalling pathway during the initiation step of the neoplasms. 20 to 40% of MM families have mutations in the tumour suppressor gene p16 or CDKN2A. In addition, somatic mutations in p16, p53, BRAF, and cKIT are present in MM. Genome-wide scan analyses on MM indicate positive associations for genes involved in melanocytic naevi, but MM is likely caused by a variety of common low-penetrance genes. Molecular dermatopathology is expanding, and its use in the assessment of melanocytic neoplasms appears to be promising in the fields of research and diagnosis. Molecular dermatopathology will probably make its way to an increased number of diagnostic laboratories. The expected benefit should improve the patient management. This evolution points to a need for evolution in the training requirements and role of dermatopathologists. [less ▲]Detailed reference viewed: 17 (6 ULg)
Dermal ultrastructure in low Beighton score members of 17 families with hypermobile-type Ehlers-Danlos syndrome.
Hermanns-Lê, Trinh ; REGINSTER, Marie-Annick ; Franchimont, Claudine et al
in Journal of Biomedicine & Biotechnology (2012), 2012
The distinction between the Ehlers-Danlos syndrome hypermobile type (EDSH) and the benign joint hypermobility syndrome (BJHS) is unclear. The aim of the present study was to compare skin ultrastructural ... [more ▼]
The distinction between the Ehlers-Danlos syndrome hypermobile type (EDSH) and the benign joint hypermobility syndrome (BJHS) is unclear. The aim of the present study was to compare skin ultrastructural abnormalities of EDSH and BJHS among different families. Skin of 23 EDSH, 27 BJHS, and 41 asymptomatic subjects from 17 families was examined using transmission electron microscopy. Similar ultrastructural abnormalities were found irrespective of the Beighton score. Flower-like collagen fibrils represented the key change and elastic fibers were altered as well. Beighton score is a clinical parameter rating joint mobility that appeared unrelated to quantitative and qualitative collagen ultrastructural alterations in the skin. Some EDSH family members fit with BJHS diagnosis. BJHS possibly represents a mild variant of EDSH. [less ▲]Detailed reference viewed: 14 (3 ULg)
Miconazole , a pharmacological barrier to skin fungal infections.
Pierard, Gérald ; Hermanns-Lê, Trinh ; Delvenne, Philippe et al
in Expert Opinion on Pharmacotherapy (2012), 13(8), 1187-94
Introduction: Miconazole (MCZ) is a time-honored antifungal of the imidazole class. MCZ exerts a multipronged effect on fungi. It inhibits the cytochrome P450 complex, including the 14alpha-demethylase ... [more ▼]
Introduction: Miconazole (MCZ) is a time-honored antifungal of the imidazole class. MCZ exerts a multipronged effect on fungi. It inhibits the cytochrome P450 complex, including the 14alpha-demethylase enzyme required for ergosterol biosynthesis, in fungal cell membranes. In addition, intracellular accumulation of toxic methylated sterols occurs and the synthesis of triglycerides and phospholipids is altered. Disturbances in oxidative and peroxidative enzyme activities lead to an intracellular toxic concentration of hydrogen peroxide. As a result, intracellular organelle destruction then leads to cell necrosis. Farnesol synthesis stimulated in Candida spp. prevents the yeast-to-mycelium formation. MCZ is further active against Gram-positive bacteria. Areas covered: This review aims at revisiting the MCZ antifungal activity in dermatomycoses. Expert opinion: MCZ's wide spectrum of activity appears noteworthy. The full pharmacological profile of MCZ indicates its fungistatic profile through its effect on ergosterol biosynthesis. In addition, it exhibits a fungicidal effect against a number of fungal species, due to hydrogen peroxide accumulation. MCZ is characterized by high safety, efficacy and versatility, and a unique, multifaceted nature of activity in the treatment of dermatomycoses. [less ▲]Detailed reference viewed: 61 (5 ULg)
Ustekinumab in psoriasis immunopathology with emphasis on the Th17-IL23 axis. A primer.
QUATRESOOZ, Pascale ; Hermanns-Lê, Trinh ; Pierard, Gérald et al
in Journal of Biomedicine & Biotechnology (2012), 2012(147413),
Psoriasis is a chronic relapsing immunoinflammatory dermatosis that is commonly associated with systemic comorbidities. The pathogenic importance of interleukin (IL)-12 and IL-23 is beyond doubt, as well ... [more ▼]
Psoriasis is a chronic relapsing immunoinflammatory dermatosis that is commonly associated with systemic comorbidities. The pathogenic importance of interleukin (IL)-12 and IL-23 is beyond doubt, as well as the involvement of T helper cells (Th)1 and Th17 cells. There is upregulation of the p40 subunit shared by IL-12 and IL-23 and of the IL-23 p19 subunit, but not an increased expression of the IL-12 p35 subunit. This indicates that IL-23 appears more involved than IL-12 in the pathogenesis of psoriatic plaques. Ustekinumab is a fully human monoclonal antibody of the immunoglobulin (Ig) G1 class targeting the p40 subunit common to both IL-12 and IL-23, thus inhibiting both IL-12 and IL-23 receptor-mediated signalling. Ustekinumab is part of the recent biologic therapies active in psoriasis, autoimmune arthritides, and inflammatory bowel diseases. [less ▲]Detailed reference viewed: 27 (3 ULg)
Field melanin mapping of the hairless scalp.
PIERARD, Gérald ; FRANCHIMONT, Claudine ; Quatresooz, Pascale
in Skin Research & Technology (2011), 18(4), 431-5
BACKGROUND: Mottled subclinical melanoderma (MSM) is frequently seen on facial skin using the ultraviolet light enhanced visualization (ULEV) method. The corresponding aspect on the hairless scalp remains ... [more ▼]
BACKGROUND: Mottled subclinical melanoderma (MSM) is frequently seen on facial skin using the ultraviolet light enhanced visualization (ULEV) method. The corresponding aspect on the hairless scalp remains unknown. OBJECTIVE: To explore the field distribution of melanin on the scalp of fair-skinned Caucasian subjects. METHOD: The scalp was examined in 43 men with androgenic alopecia. The Visioscan((R)) camera provided the ULEV pictures. Another optical (Visioface((R)) Quick) device was used under white light illumination followed by colour contrast enhancement. This was reached after specific computer filtration of the cyan hue wavelengths. RESULTS: Under white light illumination, the scalp looked normal. MSM patterns were disclosed by both optical procedures as evenly scattered discrete patchy fields of hypermelanosis. The smaller rounded spots were restricted to the lips of the hair infundibula. Larger irregularly shaped spots predominated in the interfollicular areas. A few hypomelanotic spots were scattered over the scalp. CONCLUSION: The present observations based on dual optical methods possibly provide information about a patterned pathobiology of melanocytes on the scalp. The spotty MSM pattern looked similar to the reported aspects on the face. It somewhat resembled the widespread PUVA-induced lentiginosis. [less ▲]Detailed reference viewed: 11 (3 ULg)
Hypomelanosis of Ito: pigmentary mosaicism with immature melanosome in keratinocytes.
; QUATRESOOZ, Pascale ; Hermanns-Lê, Trinh et al
in International Journal of Dermatology (2011), 50(10), 1234-9
BACKGROUND: Hypomelanosis of Ito is a rare genetic disorder characterized by whorled areas of hypomelanosis. The purpose of the present study was to revisit some aspects of Ito's hypomelanosis. METHODS ... [more ▼]
BACKGROUND: Hypomelanosis of Ito is a rare genetic disorder characterized by whorled areas of hypomelanosis. The purpose of the present study was to revisit some aspects of Ito's hypomelanosis. METHODS: Clinical observations included ultraviolet-light-enhanced visualization (ULEV) method. Histochemistry, immunohistochemistry, and electron microscopy were performed on biopsy samples from the hypopigmented areas and the surrounding skin. RESULTS: Both the ULEV and microscopic examinations revealed the heterogeneity of the pigmentation. Hypomelanosis was characterized by a reduction in melanosomes, both in melanocytes and keratinocytes. These organelles were immature and atypical, showing a weak tyrosinase immunoreactivity. Melanosome macroautophagy was prominent in keratinocytes. Some clusters of the same cells exhibited strong immunoreactivity for the Mac 387 antibody (Ca(2+) -dependent calprotectin). Ulex europaeus agglutinin-1 (UEA-1) decorated the superficial layers of the epidermis. Such features are typically found in functionally altered keratinocytes. A number of dermal cells exhibited intense phagocytic activity linked to lysozyme immunoreactivity. CONCLUSIONS: Both the melanosome depletion and macroautophagy of immature melanosomes in keratinocytes appeared to represent prominent aspects of hypomelanosis of Ito. In sum, Ito's hypomelanosis combines structural and functional changes affecting both the melanocytes and keratinocytes in the skin. [less ▲]Detailed reference viewed: 29 (2 ULg)
Intense pulsed-light therapy for proliferative superficial strawberry haemangiomas of infancy.
CAUCANAS, Marie ; PAQUET, Philippe ; HENRY, Frédérique et al
in Case Reports in Dermatological Medicine (2011), 2011(art.253607), 1-5Detailed reference viewed: 35 (5 ULg)
Traitement par anti-TNF-alpha et cancers de la peau
Pierard, Claudine ; Pierard, Gérald ; Quatresooz, Pascale et al
in ONCO-HEMATO (2011), 5Detailed reference viewed: 17 (2 ULg)
''Malignant melanoma microecosystem'': Immunohistopathological insights into the stromal cell phenotype (Review)
QUATRESOOZ, Pascale ; REGINSTER, Marie-Annick ; PIERARD, Gérald
in Experimental and Therapeutic Medicine (2011), 2Detailed reference viewed: 25 (9 ULg)