References of "Périlleux, Claire"
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See detailFLC activity is conserved between root chicory (Cichorium intybus) and Arabidopsis
Périlleux, Claire ULg

Scientific conference (2013, January 11)

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See detailAn online database for plant image analysis software tools
Lobet, Guillaume ULg; Draye, Xavier; Périlleux, Claire ULg

in Plant Methods (2013), 9(98),

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See detailA root chicory MADS-box sequence and the Arabidopsis flowering repressor FLC share common features that suggest conserved function in vernalization and devernalization responses
Périlleux, Claire ULg; Pieltain, Alexandra; Jacquemin, Guillaume et al

in Plant Journal (The) (2013), 75

Root chicory (Cichorium intybus var. sativum) is a biennial crop, but is harvested for root inulin at the end of the first growing season before flowering. However, cold temperatures might vernalize seeds ... [more ▼]

Root chicory (Cichorium intybus var. sativum) is a biennial crop, but is harvested for root inulin at the end of the first growing season before flowering. However, cold temperatures might vernalize seeds or plantlets, leading to incidental early flowering and hence understanding the molecular basis of vernalization is important. A MADS-box sequence was isolated by RT-PCR and named FLC-LIKE1 (CiFL1) because of its phylogenetic positioning within the same clade as the floral repressor Arabidopsis FLOWERING LOCUS C (AtFLC). Moreover, overexpression of CiFL1 in Arabidopsis caused late flowering and prevented up-regulation of the AtFLC target FLOWERING LOCUS T gene by photoperiod, suggesting functional conservation between root chicory and Arabidopsis. Like AtFLC in Arabidopsis, CiFL1 was repressed during vernalization of seeds or plantlets of chicory, but repression of CiFL1 was unstable whether the post-vernalization temperature was favorable to flowering or whether it devernalized the plants. Instability of CiFL1 repression might be linked to bienniality of root chicory versus the annual life cycle of Arabidopsis. However, reactivation of AtFLC was also observed in Arabidopsis when a high temperature treatment was given straight after seed vernalization, erasing the promotive effect of cold on flowering. Cold-induced downregulation of a MADS-box floral repressor and its reactivation by high temperature thus appear as conserved features of the vernalization and devernalization responses in distant species.This article is protected by copyright. All rights reserved. [less ▲]

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See detailHigh temperatures limit plant growth but hasten flowering in root chicory (Cichorium intybus) independently of vernalisation.
Mathieu, Anne-Sophie; Lutts, Stanley; Vandoorne, Bertrand et al

in Journal of Plant Physiology (2013), in press

An increase in mean and extreme summer temperatures is expected as a consequence of climate changes and this might have an impact on plant development in numerous species. Root chicory (Cichorium intybus ... [more ▼]

An increase in mean and extreme summer temperatures is expected as a consequence of climate changes and this might have an impact on plant development in numerous species. Root chicory (Cichorium intybus L.) is a major crop in northern Europe, and it is cultivated as a source of inulin. This polysaccharide is stored in the tap root during the first growing season when the plant grows as a leafy rosette, whereas bolting and flowering occur in the second year after winter vernalisation. The impact of heat stress on plant phenology, water status, photosynthesis-related parameters, and inulin content was studied in the field and under controlled phytotron conditions. In the field, plants of the Crescendo cultivar were cultivated under a closed plastic-panelled greenhouse to investigate heat-stress conditions, while the control plants were shielded with a similar, but open, structure. In the phytotrons, the Crescendo and Fredonia cultivars were exposed to high temperatures (35 °C day/ 28 °C night) and compared to control conditions (17 °C) over 10 weeks. In the field, heat reduced the root weight, the inulin content of the root and its degree of polymerisation in non-bolting plants. Flowering was observed in 12% of the heat stressed plants during the first growing season in the field. In the phytotron, the heat stress increased the total number of leaves per plant, but reduced the mean leaf area. Photosynthesis efficiency was increased in these plants, whereas osmotic potential was decreased. High temperature was also found to induce flowering of up to 50% of these plants, especially for the Fredonia cultivar. In conclusion, high temperatures induced a reduction in the growth of root chicory, although photosynthesis is not affected. Flowering was also induced, which indicates that high temperatures can partly substitute for the vernalisation requirement for the flowering of root chicory [less ▲]

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See detailEffects of cold temperatures on the early stages of maize inbreds
Riva-Roveda, Laetitia ULg; Escale, Brigitte; Giauffret, Catherine et al

Poster (2012, July)

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See detailComment les plantes fleurissent-elles ?
Périlleux, Claire ULg

Conference given outside the academic context (2012)

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See detailRooting the flowering process
D'Aloia, Maria ULg; Bouché, Frédéric ULg; Tamseddak, Karim et al

Poster (2012, May)

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See detailWhat does tomato flowering tell us about Arabidopsis ?
Périlleux, Claire ULg

Scientific conference (2012, April 23)

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See detailDegradation of recombinant IgG by root-secreted proteases of Arabidopsis thaliana and Nicotiana tabacum
Désiron, Carole ULg; Lallemand, Jérôme ULg; Périlleux, Claire ULg et al

Poster (2012, April 18)

Plants are promising hosts for the production of complex recombinant pharmaceuticals, such as antibodies (mAbs), because they offer an inexpensive and safer alternative to traditional production systems ... [more ▼]

Plants are promising hosts for the production of complex recombinant pharmaceuticals, such as antibodies (mAbs), because they offer an inexpensive and safer alternative to traditional production systems. The plant-based production of mAbs, which are multimeric glycoproteins, require their targeting to the secretory pathaway where they are properly folded and matured. However, co-secretion of endogenous proteases, which can represent up to 10% of the extracellular proteins (secretome), is known to significantly alter the yield and quality of secreted mAbs. In this study, we analyzed the proteolytic activities in root-secretome of Arabidopsis thaliana and Nicotiana tabacum. Root-secretomes were recovered by salt extraction and the protease activity was assayed in vitro or by zymography, in a range of pH. The relative contribution of protease classes was evaluated with specific inhibitors. [less ▲]

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See detailOptimization of recombinant root-secreted IgGs production in Arabidopsis thaliana by screening cell wall mutants
Boulanger, Benoit ULg; Périlleux, Claire ULg; Tocquin, Pierre ULg

Poster (2012, April 18)

The production of complex heterologous proteins (e.g. monoclonal antibodies, mAbs) in plants has several advantages animal based systems such as low cost, scalability and limited risk of contamination by ... [more ▼]

The production of complex heterologous proteins (e.g. monoclonal antibodies, mAbs) in plants has several advantages animal based systems such as low cost, scalability and limited risk of contamination by human pathogens. mAbs are glycoproteins that require to be targeted to the plant secretory pathway in order to be properly folded and matured. They are ultimately delivered in the cell wall and are expected to be freely released in the extracellular space and the external medium, which would greatly simplify downstream processing. However, a significant part of plant produced and secreted mAbs remains bound to the cell wall, therefore hindering recovery. In this study, we evaluated the extra-cellular release of root-secreted proteins of wild-type plants and cell wall mutants of Arabidopsis thaliana. Recovered protein were either analyzed by SDS-PAGE for full proteome profiling or by gelatin zymography to reveal the activity of cell wall-bound proteases. The production, secretion and release of recombinant IgG will be eventually studied in transgenic hairy-roots generated from selected mutants. [less ▲]

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See detailWater stress drastically reduces root growth and inulin yield in Cichorium intybus (var. sativum) independently of photosynthesis
Vandoorne, Bertrand; Mathieu, Anne-Sophie; Van den Ende, Wim et al

in Journal of Experimental Botany (2012), 63(12), 4359-4373

Root chicory (Cichorium intybus var. sativum) is a cash crop cultivated for inulin production in Western Europe. This plant could be exposed to severe water stress during the three last months of their ... [more ▼]

Root chicory (Cichorium intybus var. sativum) is a cash crop cultivated for inulin production in Western Europe. This plant could be exposed to severe water stress during the three last months of their six months growing period. The aim of this study was to quantify the effect of a progressive decline in water availability on plant growth, photosynthesis and sugar metabolism and to determine its impact on inulin production. Water stress drastically decreased root fresh and dry weight, leaf number, total leaf area and stomatal conductance. Stressed plants, however, increased their water use efficiency, decreased the shoot to root ratio and lowered their osmotic potential through soluble sugar accumulation. Despite a decrease in photosynthetic pigments, the light phase of the photosynthesis remained unaffected under water stress. Water stress increased sucrose phosphate synthase (SPS) activity in the leaves, but not in the roots. Water stress inhibited sucrose:sucrose 1-fructosyltransferase (1-SST) and fructan:fructan 1 fructosyltransferase (1—FFT) after 19 weeks of culture and slightly increased fructan 1-exohydrolase activities (1-FEH). The root inulin concentration and the mean degree of polymerisation (DP) of the inulin chain remained however unaffected by water stress. It is concluded that root chicory displayed resistance to water stress, but that such a resistance is obtained at the expense of growth which, in turn, leads to significant decrease in inulin production. [less ▲]

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See detailRepression of floral meristem fate is crucial in shaping tomato inflorescence
Thouet, Johanna; Quinet, Muriel; Lutts, Stanley et al

in PLoS ONE (2012), 7(2), 31096

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See detailAnalysis of Root Secreted Proteases in Arabidopsis thaliana and Nicotiana tabacum
Désiron, Carole ULg; De Lemos Esteves, Frédéric ULg; Natalis, Lucie et al

Poster (2011, June 09)

Plants are promising tools to produce complex recombinant proteins like antibodies. When host plants are grown on hydroponics, the production of recombinant proteins that are secreted by the roots ... [more ▼]

Plants are promising tools to produce complex recombinant proteins like antibodies. When host plants are grown on hydroponics, the production of recombinant proteins that are secreted by the roots ('rhizosecretion') greatly simplifies harvest and purification of the product, during whole plant life. However, proteases represent up to 10% of the naturally secreted proteins and are known to significantly decrease the yield of production by rhizosecretion. In this study, we analyzed the rhizosecreted proteases of Arabidopsis thaliana and Nicotiana tabacum. Total rhizosecreted proteins were recovered by salt extraction and the protease activity was assayed in vitro or by zymography. The relative contribution of major protease families to total activity was evaluated with specific inhibitors and revealed significant differences between the two species. The degradation capacity of the root-secreted proteases was further characterized against selected target proteins: BSA and human IgGs. [less ▲]

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See detailGenetic interactions shaping the inflorescence of tomato
Périlleux, Claire ULg

Conference (2011, June)

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See detailTranscriptomic analysis of Arabidopsis roots during floral induction by photoperiod
D'Aloia, Maria ULg; Lamoureux, Thibaut ULg; Tocquin, Pierre ULg et al

Poster (2011, June)

Contribution of the root system to the flowering process remains poorly studied. Part of the problem resides in its difficult isolation from the substrate, especially on adult plants. We used an ... [more ▼]

Contribution of the root system to the flowering process remains poorly studied. Part of the problem resides in its difficult isolation from the substrate, especially on adult plants. We used an hydroponic device that allows synchronous growth and flowering of Arabidopsis and performed global transcript profiling of roots. Samples were harvested during the extension period of a single long day (LD), and in non inductive short day. Microarray data were validated by real-time RT-PCR, and the expression patterns of selected probes were further analyzed in shoots and roots. Some of the genes that were differentially expressed in the roots during the inductive LD did not show the same variations in the shoot, indicating that root transcriptome undergoes specific changes at floral transition. These genes include, for example, GIGANTEA. T-DNA mutants from selected candidate genes are being studied. Both the expression analysis and the reverse genetic approach provide new insights into the contribution of the roots to the flowering process. [less ▲]

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See detailA cytokinin route to flowering in Arabidopsis
Bouché, Frédéric ULg; André, Julie ULg; D'Aloia, Maria ULg et al

Poster (2011, June)

Cytokinins (CKs) are involved in many physiological processes. We observed that the application of N6-benzylaminopurine (BAP) to the roots of hydroponically grown plants of Arabidopsis thaliana promotes ... [more ▼]

Cytokinins (CKs) are involved in many physiological processes. We observed that the application of N6-benzylaminopurine (BAP) to the roots of hydroponically grown plants of Arabidopsis thaliana promotes flowering in non-inductive short days. The response to BAP treatment does no require FLOWERING LOCUS T (FT), but activates its paralogue TWIN SISTER OF FT (TSF), as well as FD and SUPPRESSOR OF OVEREXPRESSION OF CO1 (SOC1) (D'Aloia et al., 2011). We present here complementary data obtained with transgenic plants overexpressing a catalytic CK OXIDASE/DEHYDROGENASE (CKX) in the roots. The high efficiency of BAP in promoting flowering in our experimental system contrasts with the variability that emerges from studies gathered in literature. Many factors, either experimental or inherent to plant material, might explain these discrepancies and we are interested in identifying endogenous regulators that might provide a mechanistic explanation. We are therefore investigating whether the endogenous pathways underlying plant developmental phase changes might regulate the relative contribution of CKs to flowering. [less ▲]

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See detailRoot Signalling at floral transition
Périlleux, Claire ULg

Conference (2011, January 27)

Detailed reference viewed: 8 (3 ULg)