References of "Périlleux, Claire"
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See detailRepression of floral meristem fate is crucial in shaping tomato inflorescence
Thouet, Johanna; Quinet, Muriel; Lutts, Stanley et al

in PLoS ONE (2012), 7(2), 31096

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See detailAnalysis of Root Secreted Proteases in Arabidopsis thaliana and Nicotiana tabacum
Désiron, Carole ULg; De Lemos Esteves, Frédéric ULg; Natalis, Lucie et al

Poster (2011, June 09)

Plants are promising tools to produce complex recombinant proteins like antibodies. When host plants are grown on hydroponics, the production of recombinant proteins that are secreted by the roots ... [more ▼]

Plants are promising tools to produce complex recombinant proteins like antibodies. When host plants are grown on hydroponics, the production of recombinant proteins that are secreted by the roots ('rhizosecretion') greatly simplifies harvest and purification of the product, during whole plant life. However, proteases represent up to 10% of the naturally secreted proteins and are known to significantly decrease the yield of production by rhizosecretion. In this study, we analyzed the rhizosecreted proteases of Arabidopsis thaliana and Nicotiana tabacum. Total rhizosecreted proteins were recovered by salt extraction and the protease activity was assayed in vitro or by zymography. The relative contribution of major protease families to total activity was evaluated with specific inhibitors and revealed significant differences between the two species. The degradation capacity of the root-secreted proteases was further characterized against selected target proteins: BSA and human IgGs. [less ▲]

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See detailGenetic interactions shaping the inflorescence of tomato
Périlleux, Claire ULg

Conference (2011, June)

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See detailTranscriptomic analysis of Arabidopsis roots during floral induction by photoperiod
D'Aloia, Maria ULg; Lamoureux, Thibaut ULg; Tocquin, Pierre ULg et al

Poster (2011, June)

Contribution of the root system to the flowering process remains poorly studied. Part of the problem resides in its difficult isolation from the substrate, especially on adult plants. We used an ... [more ▼]

Contribution of the root system to the flowering process remains poorly studied. Part of the problem resides in its difficult isolation from the substrate, especially on adult plants. We used an hydroponic device that allows synchronous growth and flowering of Arabidopsis and performed global transcript profiling of roots. Samples were harvested during the extension period of a single long day (LD), and in non inductive short day. Microarray data were validated by real-time RT-PCR, and the expression patterns of selected probes were further analyzed in shoots and roots. Some of the genes that were differentially expressed in the roots during the inductive LD did not show the same variations in the shoot, indicating that root transcriptome undergoes specific changes at floral transition. These genes include, for example, GIGANTEA. T-DNA mutants from selected candidate genes are being studied. Both the expression analysis and the reverse genetic approach provide new insights into the contribution of the roots to the flowering process. [less ▲]

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See detailA cytokinin route to flowering in Arabidopsis
Bouché, Frédéric ULg; André, Julie ULg; D'Aloia, Maria ULg et al

Poster (2011, June)

Cytokinins (CKs) are involved in many physiological processes. We observed that the application of N6-benzylaminopurine (BAP) to the roots of hydroponically grown plants of Arabidopsis thaliana promotes ... [more ▼]

Cytokinins (CKs) are involved in many physiological processes. We observed that the application of N6-benzylaminopurine (BAP) to the roots of hydroponically grown plants of Arabidopsis thaliana promotes flowering in non-inductive short days. The response to BAP treatment does no require FLOWERING LOCUS T (FT), but activates its paralogue TWIN SISTER OF FT (TSF), as well as FD and SUPPRESSOR OF OVEREXPRESSION OF CO1 (SOC1) (D'Aloia et al., 2011). We present here complementary data obtained with transgenic plants overexpressing a catalytic CK OXIDASE/DEHYDROGENASE (CKX) in the roots. The high efficiency of BAP in promoting flowering in our experimental system contrasts with the variability that emerges from studies gathered in literature. Many factors, either experimental or inherent to plant material, might explain these discrepancies and we are interested in identifying endogenous regulators that might provide a mechanistic explanation. We are therefore investigating whether the endogenous pathways underlying plant developmental phase changes might regulate the relative contribution of CKs to flowering. [less ▲]

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See detailRoot Signalling at floral transition
Périlleux, Claire ULg

Conference (2011, January 27)

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See detailCytokinin promotes flowering of Arabidopsis via transcriptional activation of the FT paralogue TSF
D'Aloia, Maria ULg; Bonhomme, Delphine ULg; Bouché, Frédéric ULg et al

in Plant Journal (The) (2011), 65

Cytokinins are involved in many aspects of plant growth and development and physiological evidence also indicates that they have a role in floral transition. In order to integrate these phytohormones into ... [more ▼]

Cytokinins are involved in many aspects of plant growth and development and physiological evidence also indicates that they have a role in floral transition. In order to integrate these phytohormones into the current knowledge of genetically defined molecular pathways to flowering, we performed exogenous treatments of adult wild-type and mutant Arabidopsis plants and analysed the expression of candidate genes. We used a hydroponic system that enables synchronous growth and flowering of Arabidopsis and allows precise application of chemicals to the roots for defined periods of time. We show that application of N6-benzylaminopurine (BAP) promotes flowering of plants grown in non-inductive short days. The response to cytokinin treatment does not require FLOWERING LOCUS T (FT) but activates its paralogue TWIN SISTER OF FT (TSF), as well as FD, which encodes a partner protein of TSF, and the downstream gene SUPPRESSOR OF OVEREXPRESSION OF CONSTANS1 (SOC1). Treatment of selected mutants confirmed that TSF and SOC1 are necessary for the flowering response to BAP while the activation cascade might partially act independently of FD. These experiments provide a mechanistic basis for the role of cytokinins in flowering and demonstrate that the redundant genes FT and TSF are differently regulated by distinct floral inducing signals. [less ▲]

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See detailThe promotive impact of high temperature on flowering in root chicory (Cichorium intybus L.)
Mathieu, Anne-Sophie; Vandoorne, Bertrand; Quinet, Muriel et al

Poster (2011)

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See detailGenetic control of flowering time in maize
Périlleux, Claire ULg; Colasanti, J.; Irish, E.

in Prioul, J.-L.; Thévenot, C.; Molnar, T. (Eds.) Advances in Maize (2011)

Flowering in temperate maize occurs largely autonomously after the plant has accumulated a given amount of vegetative growth. Mutants affected in leaf initiation rate or in phyllotaxy however indicate ... [more ▼]

Flowering in temperate maize occurs largely autonomously after the plant has accumulated a given amount of vegetative growth. Mutants affected in leaf initiation rate or in phyllotaxy however indicate that total leaf number can vary independently of flowering time, e.g. in relation with cytokinin signalling. By contrast, heterochronic mutants in which juvenile-to-adult and/or adult vegetative-to-reproductive phase changes are abnormal aided in the identification of key regulators of endogenous developmental timing in maize. These regulators include gibberellins and micro-RNAs, such as miR156 and miR172, which have been identified more recently. Progress towards unravelling the maize flowering time genetic network is also emerging from comparison with other species. Although maize expansion beyond domestication centres implied reduction in photoperiod sensitivity, molecular genetic studies indicated conservation of genes which, in Arabidopsis or rice, act in a signalling cascade whereby flowering is controlled by photoperiod. Several gene sequences are now available to assess functionality of such a pathway in maize and evaluate its contribution to flowering time control. [less ▲]

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See detailTranscriptomic analysis of Arabidopsis roots during flowering
D'Aloia, Maria ULg; Tocquin, Pierre ULg; Périlleux, Claire ULg

Poster (2010, February)

Contribution of the root system to the flowering process remains poorly studied. Part of the problem resides in its difficult isolation from the substrate, especially on adult plants. Taking advantage of ... [more ▼]

Contribution of the root system to the flowering process remains poorly studied. Part of the problem resides in its difficult isolation from the substrate, especially on adult plants. Taking advantage of an hydroponic device that allows synchronous growth and flowering of Arabidopsis thaliana (Tocquin et al., 2003), we performed global transcript profiling of roots during induction of flowering by a single long day (LD). Results were validated by real-time RT-PCR, and the expression patterns of selected probes were further analyzed in shoots and roots. Some of the genes that were identified in the microarray experiment were already known to be involved in the photoperiodic pathway of flowering in Arabidopsis, and hence were activated in both roots and shoots during the LD. These genes include, for example, components of light signaling or circadian machinery (e.g. GIGANTEA). Other genes providing new insights into the control of flowering at the whole plant level will be presented. Tocquin et al., (2003). BMC Plant Biology, 3: 2. [less ▲]

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See detailPhysiologie végétale - BAC3 Bio
Périlleux, Claire ULg

Learning material (2010)

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See detailBiologie du développement - partim. végétal / Master 1 BBMC
Périlleux, Claire ULg

Learning material (2010)

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See detailL'évolution des plantes cultivées: une histoire peu naturelle ... de la domestication aux OGM
Périlleux, Claire ULg

Conference given outside the academic context (2009)

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See detailPartenariat F.N.P.S.M.S. - ULg / Rapport ULg 2008
Périlleux, Claire ULg; Van Kerkhoven, Fabrizio; Gonzalez, Arnaud

Report (2009)

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See detailFunctional analysis of an FLC-LIKE gene in root chicory
Périlleux, Claire ULg; Pieltain, Alexandra ULg; D'Aloia, Maria ULg et al

in Comparative Biochemistry and Physiology. Part A, Physiology (2009), 153A(2/Suppl.), 198-199

Vernalization is known to promote flowering in Arabidopsis thaliana by inhibiting the expression of a strong repressor: FLOWERING LOCUS C (FLC). The recent cloning of an FLC-LIKE gene in sugar beet (Beta ... [more ▼]

Vernalization is known to promote flowering in Arabidopsis thaliana by inhibiting the expression of a strong repressor: FLOWERING LOCUS C (FLC). The recent cloning of an FLC-LIKE gene in sugar beet (Beta vulgaris; BvFL1) and – here – in root chicory (Cichorium intybus; CiFL1) suggests the conservation of FLC biological function during evolution of eudicots. Hence physiological questions that remain difficult to address in Arabidopsis can be studied in other species. We investigated the correlation between CiFL1 expression and plant-age dependent responsiveness to vernalization. We also studied the effect of post-vernalization growing temperature, which can stabilize or erase the vernalized state. [less ▲]

Detailed reference viewed: 71 (25 ULg)