References of "Ongena, MARC"
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See detailNatural functions of cyclic lipopeptides from Bacillus and Pseudomonas: more than surfactants and antibiotics
Raaijmakers, Jos; De Bruin, Irene; Nybroe, Ole et al

in FEMS Microbiology Reviews (2010), 34

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See detailBeneficial effects of Bacillus subtilis on field-grown tomato in Burundi : reduction of local Fusarium disease and growth promotion.
Nihorimbere, V.; Ongena, Marc ULg; Cawoy, Hélène ULg et al

in African Journal of Microbiology Research [=AJMR] (2010), 4

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See detailProduction of three anti-listerial peptides by Lactobacillus curvatus in MRS broth.
Ghalfi, H.; Benkerroum, N.; Ongena, Marc ULg et al

in Food Research International (2010), (43), 33-39

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See detailInsights into the plant defense mechanisms induced by Bacillus lipopeptides.
Ongena, Marc ULg; Henry, Guillaume ULg; Adam, Akram et al

Poster (2009, July)

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See detailPilot scale biotransformation of vegetal oil into natural green note flavor using sugar beet leaves as sources of hydroperoxide lyase
Gigot, Cédric ULg; Ongena, Marc ULg; Fauconnier, Marie-Laure ULg et al

Poster (2009, June 02)

Natural green note aromas (GLVs) are highly attractive flavors commonly used in the food industry. These are produced in extremely low levels upon physiological stress in plant organs of any sort. This ... [more ▼]

Natural green note aromas (GLVs) are highly attractive flavors commonly used in the food industry. These are produced in extremely low levels upon physiological stress in plant organs of any sort. This weak sporadic presence entails a very expensive extraction step to obtain pure GLVs. Therefore catalytic biotransformations of fatty acid sources, the initial substrate for GLVs, have been developed. Enzymatic defense pathways and particularly the LOX pathway produce the major part of GLVs. Unlike GLV molecules that are emitted in the atmosphere, the enzymes are extractible from the plant material. Thus, a combination of plant enzyme extracts and substrate preparations provides all the ingredients for GLV production. Besides, sugar beet leaves present high levels of hydroperoxide lyase among plant sources and are available in large amounts during three months. In this enzymatic pathway, fatty acids are successively transformed by lipase, lipoxygenase and hydroperoxide lyase into aldehydes and alcohols, final compounds of GLVs pathway. Limiting and problematic steps occur with the action of hydroperoxide lyase, when enzymatic catalysis is followed by an enzyme destabilization. Alternative substrates bind irreversibly to the heme group of the enzyme and end the reaction. This poster briefly describes the development of a complete bioprocess for natural GLV production, from hydrolysis to purification. A high level of biotransformation could be achieved using optimum experimental conditions and a cheap source of plant materials. [less ▲]

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See detailBacillus-based biocontrol of Fusarium disease on tomato cultures in Burundi
Nihorimbere, Venant; Ongena, Marc ULg; Cawoy, Hélène ULg et al

Poster (2009, May 19)

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See detailPlant growth promotion of tomato under field conditions in Burundi
Cawoy, Hélène ULg; Nihorimbere, Venant; Kakana, Pascal et al

Poster (2009, April 02)

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See detailBacillus amyloliquefaciens GA1 as a source of potent antibiotics and other secondary metabolites for biocontrol of plant pathogens.
Arguelles-Arias, A.; Ongena, MARC ULg; Halimi, B. et al

in Microbial Cell Factories (2009), 8(1), 63

ABSTRACT: BACKGROUND: Phytopathogenic fungi affecting crop and post-harvested vegetables are a major threat to food production and food storage. To face these drawbacks, producers have become increasingly ... [more ▼]

ABSTRACT: BACKGROUND: Phytopathogenic fungi affecting crop and post-harvested vegetables are a major threat to food production and food storage. To face these drawbacks, producers have become increasingly dependent on agrochemicals. However, intensive use of these compounds has led to the emergence of pathogen resistance and severe negative environmental impacts. There are also a number of plant diseases for which chemical solutions are ineffective or non-existent as well as an increasing demand by consumers for pesticide-free food. Thus, biological control through the use of natural antagonistic microorganisms has emerged as a promising alternative to chemical pesticides for more rational and safe crop management. RESULTS: The genome of the plant-associated B. amyloliquefaciens GA1 was sample sequenced. Several gene clusters involved in the synthesis of biocontrol agents were detected. Four gene clusters were shown to direct the synthesis of the cyclic lipopeptides surfactin, iturin A and fengycin as well as the iron-siderophore bacillibactin. Beside these non-ribosomaly synthetised peptides, three additional gene clusters directing the synthesis of the antibacterial polyketides macrolactin, bacillaene and difficidin were identified. Mass spectrometry analysis of culture supernatants led to the identification of these secondary metabolites, hence demonstrating that the corresponding biosynthetic gene clusters are functional in strain GA1. In addition, genes encoding enzymes involved in synthesis and export of the dipeptide antibiotic bacilysin were highlighted. However, only its chlorinated derivative, chlorotetaine, could be detected in culture supernatants. On the contrary, genes involved in ribosome-dependent synthesis of bacteriocin and other antibiotic peptides were not detected as compared to the reference strain B. amyloliquefaciens FZB42. CONCLUSION: The production of all of these antibiotic compounds highlights B. amyloliquefaciens GA1 as a good candidate for the development of biocontrol agents. [less ▲]

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See detailEcological fitness of Bacillus subtilis BGS3 regarding production of the surfactin lipopeptide in the rhizosphere
Nihorimbere, Venant ULg; Fickers, Patrick ULg; Thonart, Philippe ULg et al

in Environmental Microbiology Reports (2009), 1(2), 124-130

Cyclic lipopeptides and particularly surfactins produced by Bacillus species retain antibacterial, antiviral, biofilm-forming and plant resistance-inducing activities. In most cases, their role in ... [more ▼]

Cyclic lipopeptides and particularly surfactins produced by Bacillus species retain antibacterial, antiviral, biofilm-forming and plant resistance-inducing activities. In most cases, their role in biological control of plant diseases was evoked on the basis of in vitro assays or by using non-producing/ overproducing mutants but there is a need for more direct evidence of an efficient lipopeptide biosynthesis in the rhizosphere. In this work, we coupled LC-MS quantification of the lipopeptides secreted by cells colonizing tomato plants with the use of psrfA– lacZ reporter system integrated within the BGS3 chromosome to study the expression of the surfactin operon in planta. Results showed that a higher level of psrfA induction was observed upon the establishment of a stable BGS3 population on roots and surfactins extracted from the rhizosphere were produced in biologically significant quantities. Our results also demonstrate that BGS3 efficiently utilizes the main substrates from plant exudates to produce surfactins. This synthesis is also efficient in cells forming colonies and the production may be favoured in bacteria developing slowly in the rhizosphere. This provides a first understanding of how environmental factors may influence lipopeptide production by beneficial Bacillus strains. [less ▲]

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See detailPlant defense reactions stimulated following perception of Bacillus lipopeptides
Ongena, Marc ULg; Henry, G.; Jourdan, E. et al

Conference (2009)

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See detailInfluence of rhizosphere-specific parameters on surfactin production by Bacillus subtilis.
Ongena, MARC ULg; Nihorimbere, Venant; Fickers, Patrick ULg et al

in Bulletin OILB/SROP = IOBC/WPRS Bulletin (2009), (43), 317-320

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See detailBacillus lipopeptides as MAMPs for non-pathogenic bacteria perception and defense responses elicitation in plant cells.
Henry, Guillaume; Ongena, MARC ULg; Jourdan, Emmanuel ULg et al

in Bulletin OILB/SROP = IOBC/WPRS Bulletin (2009), 43

Detailed reference viewed: 77 (36 ULg)