Plant defense reactions stimulated following perception of Bacillus lipopeptides

Conference (2009)

Plant defense reactions stimulated following perception of elicitors from beneficial rhizobacteria

Conference (2009)

Bacillus lipopeptides as MAMPs for non-pathogenic bacteria perception and defense responses elicitation in plant cells.

in Bulletin OILB/SROP = IOBC/WPRS Bulletin (2009), 43

Insights into the Defense-Related Events Occuring in Plant Cells Following Perception of Surfactin-Type Lipopeptide from Bacillus subtilis

in Molecular Plant-Microbe Interactions [=MPMI] (2009), 22(4), 456-468

Multiple strains of Bacillus subtilis were demonstrated to stimulate plant defense responses, and cyclic lipopeptides may be involved in the elicitation of this induced systemic resistance phenomenon ... [more ▼]

Multiple strains of Bacillus subtilis were demonstrated to stimulate plant defense responses, and cyclic lipopeptides may be involved in the elicitation of this induced systemic resistance phenomenon. Here, we further investigated molecular events underlying the interaction between wuch lipopeptides and plant cells. Addition of surfactin but not fengycin or iturin in the micromolar range to tobacco cell suspensions induced defense-related early events such as extracellular medium alkalinization coupled with ion fluxes and reactive oxygen species production. Surfactin also stimulated the defense enzymes phenylalanine ammonia lyase and lipoxygenase and modified the pattern of phenolics produced by the elicited cells. The occurence of these surfactin-elicited early events is closely related to Ca2+ influx and dynamic changes in protein phosphorylation but is not associed with any marked phytotoxicity or adverse effect on the integrity and growth potential of the treated tobacco cells. [less ▲]

Bacillus amyloliquefaciens GA1 as a source of potent antibiotics and other secondary metabolites for biocontrol of plant pathogens.

in Microbial Cell Factories (2009), 8(1), 63

ABSTRACT: BACKGROUND: Phytopathogenic fungi affecting crop and post-harvested vegetables are a major threat to food production and food storage. To face these drawbacks, producers have become increasingly ... [more ▼]

ABSTRACT: BACKGROUND: Phytopathogenic fungi affecting crop and post-harvested vegetables are a major threat to food production and food storage. To face these drawbacks, producers have become increasingly dependent on agrochemicals. However, intensive use of these compounds has led to the emergence of pathogen resistance and severe negative environmental impacts. There are also a number of plant diseases for which chemical solutions are ineffective or non-existent as well as an increasing demand by consumers for pesticide-free food. Thus, biological control through the use of natural antagonistic microorganisms has emerged as a promising alternative to chemical pesticides for more rational and safe crop management. RESULTS: The genome of the plant-associated B. amyloliquefaciens GA1 was sample sequenced. Several gene clusters involved in the synthesis of biocontrol agents were detected. Four gene clusters were shown to direct the synthesis of the cyclic lipopeptides surfactin, iturin A and fengycin as well as the iron-siderophore bacillibactin. Beside these non-ribosomaly synthetised peptides, three additional gene clusters directing the synthesis of the antibacterial polyketides macrolactin, bacillaene and difficidin were identified. Mass spectrometry analysis of culture supernatants led to the identification of these secondary metabolites, hence demonstrating that the corresponding biosynthetic gene clusters are functional in strain GA1. In addition, genes encoding enzymes involved in synthesis and export of the dipeptide antibiotic bacilysin were highlighted. However, only its chlorinated derivative, chlorotetaine, could be detected in culture supernatants. On the contrary, genes involved in ribosome-dependent synthesis of bacteriocin and other antibiotic peptides were not detected as compared to the reference strain B. amyloliquefaciens FZB42. CONCLUSION: The production of all of these antibiotic compounds highlights B. amyloliquefaciens GA1 as a good candidate for the development of biocontrol agents. [less ▲]

Influence of rhizosphere-specific parameters on surfactin production by Bacillus subtilis.

Conference (2008, September)

Ecological fitness of plant beneficial Bacillus subtilis strains in soil: influence of rhizosphere-specific parameters on surfactin synthesis

Poster (2008, August)

Valorisation des produits issus des feuilles de betteraves : extraction de molécules à note verte - Rapport final

Report (2008)

Systemic resistance induction in tomato by Pseudomonas putida BTP1: investigation of defense pathways.

in Journal of Plant Pathology [=JPP] (2008), 90

Defence responses induced in tobacco cells by Bacillus lipopeptides acting as elicitors of systemic resistance in plants

in Journal of Plant Pathology [=JPP] (2008), 90