References of "NISOLLE, Michelle"
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See detailComment je traite...un uterus didelphe associe a un hemi-vagin borgne
Loumaye, F.; Cheruy, Charlotte ULg; Cristinelli, S. et al

in Revue Médicale de Liège (2007), 62(7-8, Jul-Aug), 480-3

Didelphys uterus with imperforated obstructed hemivagina is a rare condition. Most often, it is diagnosed a few months after the menarche. Hematocolpos, hematometria and sometimes hematosalpinx are ... [more ▼]

Didelphys uterus with imperforated obstructed hemivagina is a rare condition. Most often, it is diagnosed a few months after the menarche. Hematocolpos, hematometria and sometimes hematosalpinx are responsible for pelvic pain and dysmenorrhea. Symptoms can be delayed when a fistulisation from the hematocolpos to permeable contralateral vagina is present. Sometimes, diagnosis is not made before adulthood during an infection of the hematocolpos. Useful additional exams include pelvic ultrasound and, in some cases, MRI, which is the best exam to precisely describe the type of malformation. An ipsilateral agenesia of the kidney is always associated. Hysterography can usually demonstrate the fistulous courses. Treatment consists in a large resection of the vaginal septum in order to allow a permanent drainage of the hemiuterus. A laparoscopy should be performed to check for the presence of associated tubal or pelvic damage. [less ▲]

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See detailComment je traite ... une grossesse extra-uterine
Degee, S.; Dricot, J. F.; Lamborelle, S. et al

in Revue Médicale de Liège (2006), 61(12), 797-803

Nowadays, the management of ectopic pregnancy should be codified as often as possible. Two types of treatment are possible. One surgical and the other medical. Our study is concerned with a series of 97 ... [more ▼]

Nowadays, the management of ectopic pregnancy should be codified as often as possible. Two types of treatment are possible. One surgical and the other medical. Our study is concerned with a series of 97 patients hospitalized for suspicion of ectopic pregnancy for whom the risk factors and treatment were analyzed. Our conclusion is that the medical treatment should be more frequently proposed and surgery should prefer the conservative treatment whenever it is still possible. [less ▲]

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See detailLe cas clinique du mois. Sterilet en position annexielle: une complication rare
Cristinelli, S.; Nisolle, Michelle ULg; Foidart, Jean-Michel ULg

in Revue Médicale de Liège (2006), 61(11), 747-9

The intrauterine device (IUD) is a widely used, effective and reversible means of birth control. Its insertion can be associated with perforation of the utuerus. This is a rare complication, but with ... [more ▼]

The intrauterine device (IUD) is a widely used, effective and reversible means of birth control. Its insertion can be associated with perforation of the utuerus. This is a rare complication, but with possible serious clinical consequences. There is an increased risk of uterine perforation if the IUD is inserted during 6 months of post-partum or after uterine evacuation. A follow up by sonography is essential. The surgical removal of the ectopic IUD is imperious. [less ▲]

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See detailL'image du mois. Une malformation uterine evoquant une masse annexielle
Wauters, Odile ULg; Pintiaux, Axelle ULg; Foidart, Jean-Michel ULg et al

in Revue Médicale de Liège (2006), 61(10), 665-6

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See detailLe cas clinique du mois: un dysgerminome bilateral: une presentation rare du syndrome de Swyer
Nisolle, Michelle ULg; Kridelka, Frédéric ULg; Fridman, Viviana ULg et al

in Revue Médicale de Liège (2005), 60(9), 703-6

Swyer syndrome is a pure gonad dysgenesis associating 46 XY karyotype, primary amenorrhea, presence of female internal genital tract and bilateral streak gonads in a phenotypic female. The diagnosis is ... [more ▼]

Swyer syndrome is a pure gonad dysgenesis associating 46 XY karyotype, primary amenorrhea, presence of female internal genital tract and bilateral streak gonads in a phenotypic female. The diagnosis is usually made at adolescence when the primary amenorrhea is investigated. We report the case of a 25-year-old XY woman who developed a bilateral dysgerminoma from undifferentiated gonads. [less ▲]

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See detailEndometriose stade I et II: implications physiopathologiques, cliniques et therapeutiques
Nisolle, Michelle ULg; Nervo, Patricia ULg

in Journal de Gynécologie, Obstétrique et Biologie de la Reproduction (2003), 32(8, Pt 2), 11-4

Stage I and II endometriosis is defined by a r-AFS score respectively ranging from 1 to 5 and from 6 to 15. This mild, superficial endometriosis is a very common pathology occurring in infertile women ... [more ▼]

Stage I and II endometriosis is defined by a r-AFS score respectively ranging from 1 to 5 and from 6 to 15. This mild, superficial endometriosis is a very common pathology occurring in infertile women. Nevertheless, these women with stage I/II endometriosis have usually few pelvic pain. This review summarizes the recent literature concerning new data on the pathogenesis of peritoneal endometriosis and its clinical management. Retrograde menstruation, peritoneal adhesion of shed endometrial tissue, and outgrowth of endometrial cells, glands and stroma, are essential elements in the pathogenesis of endometriosis according to Sampson's classic implantation theory. Nevertheless, exact pathophysiology of endometriosis remains unknown. Superficial endometriotic lesions observed by laparoscopy have to be treated. Surgical procedure is not difficult for stage I and II of endometriosis. Surgical procedure remains controversial. Carbon dioxide (CO2) Laser can be used for laparoscopic destruction of endometriosis. Newer procedures, such as SurgiTouch (Lumenis), are more effective in vaporization and decrease the risk thermal damage of contiguous structures. The monopolar scissors can also be used in order to excise the peritoneal endometriotic lesions. Medical treatment may be usefull if surgical treatment is not complete or if the pelvic cavity is hypervascularized. In these cases, Gonadotropin-Releasing Hormone agonists (Gn-RHa) are the most common and effective treatment. [less ▲]

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See detailIs laparoscopy still the gold standard in infertility assessment? A comparison of fertiloscopy versus laparoscopy in infertility. Results of an international multicentre prospective trial: the 'FLY' (Fertiloscopy-LaparoscopY) study.
Watrelot, A.; NISOLLE, Michelle ULg; Chelli, H. et al

in Human Reproduction (2003), 18(4), 834-9

BACKGROUND: The aim of this prospective multicentre study was to compare the two endoscopic techniques of laparoscopy and fertiloscopy in routine evaluation of the pelvis in infertile women. METHODS: A ... [more ▼]

BACKGROUND: The aim of this prospective multicentre study was to compare the two endoscopic techniques of laparoscopy and fertiloscopy in routine evaluation of the pelvis in infertile women. METHODS: A total of 92 women was selected in 14 University Hospitals to undergo fertiloscopy followed by transabdominal laparoscopy by a team of two surgeons in each hospital. RESULTS: A high degree of concordance was observed between these two techniques, in that if fertiloscopy did not detect any abnormalities, this was also confirmed by laparoscopy. Discordance was observed in similar numbers of cases: eight after laparoscopy and nine after fertiloscopy. The diagnostic index for fertiloscopy and laparoscopy was calculated; sensitivity (86 and 87% respectively) and negative predictive value (64 and 67% respectively) were similar. The kappa index was also calculated for each of the six structures/regions (right/left tube; right/left ovary; peritoneum of pouch of Douglas; posterior uterus), and concordance (0.78 to 0.91) was considered almost complete. CONCLUSIONS: These results confirm fertiloscopy as a minimally invasive safe procedure that may be considered as an alternative to diagnostic laparoscopy in the routine assessment of women without clinical or ultrasound evidence of pelvic disease. On the basis of the additional advantages of fertiloscopy, namely salpingoscopy or microsalpingoscopy, it is considered that fertiloscopy could replace laparoscopy as a routine procedure in such women. [less ▲]

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See detailOvarian endometriosis and peritoneal endometriosis: are they different entities from a fertility perspective?
Nisolle, Michelle ULg

in Current Opinion in Obstetrics & Gynecology (2002), 14(3), 283-288

This review summarizes the recent literature concerning new data on the pathogenesis of peritoneal endometriosis and its natural evolution. Indeed, the main concern in endometriosis is the choice of ... [more ▼]

This review summarizes the recent literature concerning new data on the pathogenesis of peritoneal endometriosis and its natural evolution. Indeed, the main concern in endometriosis is the choice of treatment in cases of infertility problems: medical or surgical. This concern could hypothetically be avoided if endometriosis were considered as a spontaneously regressive phenomenon. The present paper also discusses the risk of recurrence and the results of in-vitro fertilization and embryo transfer in cases of ovarian endometriosis. Whatever type of surgery is performed, the results of in-vitro fertilization and embryo transfer are not impaired, especially if damage to the ovarian cortex is avoided. Further studies are required to determine if oocytes from endometriosis patients are altered and could be responsible for the development of lower quality embryos. [less ▲]

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See detailUreteral endometriosis: a complication of rectovaginal endometriotic (adenomyotic) nodules
Donnez, Jacques; NISOLLE, Michelle ULg; Squifflet, Jean

in Fertility and Sterility (2002), 77(1), 32-37

Objective: To present data from 18 cases of ureteral endometriosis. Design: Prospective clinical study. Setting: Department of gynecology at a university hospital. Patient(s): Four hundred and five ... [more ▼]

Objective: To present data from 18 cases of ureteral endometriosis. Design: Prospective clinical study. Setting: Department of gynecology at a university hospital. Patient(s): Four hundred and five patients with severe dysmenorrhea or deep dyspareunia due to a rectovaginal endometriotic (adenomyotic) nodule. Intervention(s): Patients were prospectively evaluated using intravenous pyelography. All patients underwent laparoscopic surgery to remove rectovaginal adenomyosis and ureterolysis. Main Outcome Measure(s): Presurgical and postsurgical evaluation and histologic analysis. Result(s): Preoperative intravenous pyelography revealed ureteral stenosis with ureterohydronephrosis in 18 patients (4.4%). A significantly higher prevalence (11.2%) was observed in nodules ≥ 3 cm in diameter. Five women (20%) had complete ureteral stenosis. Kidney scintigraphy revealed damaged kidney parenchymal function, which ranged from 18% to 42%. Laparoscopic ureterolysis was done in 16 women; 2 women underwent ureteral resection and uretero-ureterostomy. A significant postoperative decrease in ureterohydronephrosis was noted in all patients; however, renal function improved only slightly. Conclusion(s): Ureteral endometriosis was found in 4.4% of patients with rectovaginal endometriotic (adenomyotic) nodules. Ureterolysis and removal of associated adenomyotic lesions was sufficient therapy in most patients; two required resection of the ureteral stenotic segment. Intravenous pyelography should be performed in all women with rectovaginal nodules ≥ 3 cm to prevent nonreversible loss of renal function. [less ▲]

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See detailThe efficacy of medical and surgical treatment of endometriosis-associated infertility: arguments in favour of a medico-surgical aproach
Donnez, Jacques; Chantraine, Frédéric ULg; Nisolle, Michelle ULg

in Human Reproduction Update (2002), 8(1), 89-94

This review discusses the efficacy of a combined, medical (GnRH agonist) and surgical, therapy in endometriosis- associated infertility. Because of the limited information currently available on the ... [more ▼]

This review discusses the efficacy of a combined, medical (GnRH agonist) and surgical, therapy in endometriosis- associated infertility. Because of the limited information currently available on the activity of lesions in minimal and mild endometriosis, any absolute statement is inappropriate at this time, although some arguments exist in favour of treating endometriosis at laparoscopy. In moderate and severe endometriosis, this review provides arguments in favour of a medico-surgical approach and discusses the possibility of combining medical and surgical therapy. [less ▲]

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See detailAttachment of “menstrual” endometrium to peritoneum?
Donnez, Jacques; NISOLLE, Michelle ULg

in Fertility and Sterility (2001), 76(3), 642

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See detailDoes ovarian surgery for endometriomas impair the ovarian response to gonadotropin?
Donnez, Jacques; Wyns, Christine; NISOLLE, Michelle ULg

in Fertility and Sterility (2001), 76(4), 662-5

Objective: To evaluate the ovarian response to stimulation conducted for IVF treatment in women who have undergone conservative surgery for endometriomas. Design: Retrospective study with prospective ... [more ▼]

Objective: To evaluate the ovarian response to stimulation conducted for IVF treatment in women who have undergone conservative surgery for endometriomas. Design: Retrospective study with prospective selection of participants and controls. Setting: University infertility clinic. Patient(s): A series of 374 women who underwent in vitro fertilization (IVF). The study group consisted of 85 patients with ovarian endometriomas who had undergone laparoscopic surgery in an attempt to become pregnant, but had failed within a year of surgery. The control group consisted of 289 patients with tubal factor infertility. Intervention(s): IVF-embryo transfer procedures. Main Outcome Measure(s): Stimulation parameters, fertilization, implantation, and pregnancy rates were analyzed in both groups. Result(s): There was no significant difference between the two groups in stimulation parameters or IVF outcome. Conclusion(s): A total of 820 cycles were analyzed. A similar IVF-ET outcome was observed in patients with endometriosis after ablation of endometriomas compared to women with tubal factors. In conclusion, endometrioma surgery by internal wall vaporization does not impair IVF outcome. The clinical pregnancy rate was respectively 37.4% and 34.6% in the endometriosis group and the control group. [less ▲]

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See detailComparison of G1.2/G2.2 and Sydney IVF cleavage/blastocyst sequential media for the culture of human embryos: a prospective, randomized, comparative study.
Van Langendonckt, Anne; Demylle, Dominique; Wyns, Christine et al

in Fertility and Sterility (2001), 76(5), 1023-31

Objective: To compare two commercially available sequential media, G1.2/G2.2 and Sydney IVF cleavage/blastocyst media, as supports for human embryo culture. Design: Prospective randomized study. Setting ... [more ▼]

Objective: To compare two commercially available sequential media, G1.2/G2.2 and Sydney IVF cleavage/blastocyst media, as supports for human embryo culture. Design: Prospective randomized study. Setting: University-based IVF clinic. Patient(s): Two hundred forty-nine patients undergoing IVF treatment for the first or second time, randomly allocated at the time of oocyte retrieval, to either culture in G1.2/G2.2 or Sydney IVF media. Intervention(s): Oocyte recovery, IVF or intracytoplasmic sperm injection, embryo culture, transfer on day 3 or day 5/6. Main Outcome Measure(s): Developmental stage on day 3, blastocyst rate, pregnancy outcome as assessed by βhCG positive test, implantation rates, and ongoing pregnancies. Result(s): Embryos cultured in G1.2/G2.2 media displayed a faster kinetics of cleavage, compaction, blastulation, and hatching, but a lower day 3 embryo quality than those grown in Sydney IVF media. For patients with at least five embryos, G1.2/G2.2 media yielded higher implantation rates (26.2%) in our day 3 embryo transfer program when compared to Sydney IVF medium (15.5%), whereas similar implantation rates were obtained for day 5/6 embryo transfer for both media (43.1% and 36.1%, respectively). Conclusion(s): In our day 3 embryo transfer program, G1.2/G2.2 media were superior to Sydney IVF media, whereas both media yielded similar outcomes in our blastocyst transfer program. [less ▲]

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See detailDistribution and epidermal growth factor receptor expression of primordial follicles in human ovarian tissue before and after cryopreservation.
Qu, Jian Ping; Godin, Pierre-Arnaud; NISOLLE, Michelle ULg et al

in Human Reproduction (2000), 15(2), 303-10

The freezing of ovarian tissue and the growth of immature oocytes from primordial follicles is an interesting concept in ovarian tissue transplantation and in-vitro fertilization. In this study, the ... [more ▼]

The freezing of ovarian tissue and the growth of immature oocytes from primordial follicles is an interesting concept in ovarian tissue transplantation and in-vitro fertilization. In this study, the morphology and distribution of primordial follicles were studied in ovarian tissue from 24 women before and after cryopreservation. Cryopreservation did not significantly change either the morphology or number per unit volume of morphologically normal follicles in frozen ovarian tissue. Primordial follicles were predominant, accounting for 78.6% and 82.6% of total follicles in fresh and frozen ovarian tissues respectively. The distribution of follicles was extremely uneven in ovarian tissue. A large variation in follicle numbers was observed in ovarian tissue samples from patient to patient, and even in the same patient, indicating that the number of follicles counted in one sample of ovarian tissue may not represent the number of follicles in other tissue samples. Ovarian tissue could be frozen in the form of strips instead of fragments for fast processing and better viability of ovarian tissue in cryopreservation. The number of follicles in ovarian tissue declined with the increasing age of the patients. An immunohistochemical study showed that immunoreactivity for the epidermal growth factor (EGF) receptor was detected in primordial follicles of adult ovarian tissue. EGF receptor staining was most intense in the oocytes of primordial follicles. Weak staining for EGF receptor was observed in some surrounding pregranulosa cells. Immunohistochemical staining for EGF receptor was also present in the stromal cells of ovarian tissue, but to a much lesser degree. There was no significant difference in the immunohistochemical staining for EGF receptor in ovarian tissue before and after cryopreservation. [less ▲]

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See detailExpression of receptors for insulin-like growth factor-I and transforming growth factor-beta in human follicles.
Qu, Jian Ping; GODIN, Pierre-Arnaud ULg; NISOLLE, Michelle ULg et al

in Molecular Human Reproduction (2000), 6(2), 137-45

The in-vitro growth of immature oocytes in early follicles from cryopreserved human ovarian tissues is a new concept in in-vitro fertilization programmes for the treatment of infertile and cancer patients ... [more ▼]

The in-vitro growth of immature oocytes in early follicles from cryopreserved human ovarian tissues is a new concept in in-vitro fertilization programmes for the treatment of infertile and cancer patients. To better understand the regulatory mechanism of follicular development, immunohistochemistry was used to study the expression of insulin-like growth factor (IGF) type I receptor (IGF-IR) and transforming growth factor-β (TGFβ) type I (TβR-I) and type II (TβR-II) receptors in fresh and frozen ovarian tissues from 14 women. Immunoreactivities for IGF-IR and TβR-I were present simultaneously in the oocytes of primordial, pre-antral and antral follicles. Staining for both IGF-IR and TβR-I was also observed in granulosa cells of primordial, pre-antral and antral follicles. IGF-IR and TβR-I also stained in thecal cells of pre-antral and antral follicles. Stromal cells in surrounding ovarian tissue expressed IGF-IR and TβR-I at various follicular stages. Unlike TβR-I, TβR-II was expressed only in the oocytes of primordial and primary follicles, and with weak staining intensity in thecal cells. No significant staining for TβR-II was found in oocytes and granulosa cells of antral follicles. There was no difference in staining patterns for IGF-IR, TβR-I and TβR-II between fresh and frozen ovarian tissues, indicating that cryopreservation might not significantly alter the immunoreactivities of these receptors in frozen ovarian tissue. The results suggest that IGF-I and TGFβ may participate in the regulation of follicular growth by binding to their receptors through an autocrine or paracrine mechanism. IGF-I and TGFβ may be useful in regulating the in-vitro or in-vivo maturation of oocytes not only in later follicles but also very early follicles, from cryopreserved ovarian tissues for clinical use in the future. [less ▲]

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See detailPredictive value of ovarian endometriosis?
NISOLLE, Michelle ULg; Donnez, Jacques

in Fertility and Sterility (2000), 73(2), 419-20

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See detailTransplantation of cultured explants of human endometrium into nude mice
NISOLLE, Michelle ULg; Casanas-Roux, Françoise; Marbaix, Etienne et al

in Human Reproduction (2000), 15(3), 572-7

The objective of this study was to analyse the histological and immunohistochemical characteristics of cultured explants of human endometrium transplanted into nude mice. Biopsies of eutopic endometrium ... [more ▼]

The objective of this study was to analyse the histological and immunohistochemical characteristics of cultured explants of human endometrium transplanted into nude mice. Biopsies of eutopic endometrium were taken from six patients during laparoscopic surgery and classified according to the phase of the cycle. All the explants were cultured with oestrogen and progesterone for 24 h before transplantation into 15 mice. Four mice were grafted with explants of menstrual endometrium, four with explants of late proliferative endometrium, and seven with explants of late secretory-premenstrual endometrium. Typical endometrial glands and stroma were observed in 87% of cases 3 weeks after the transplantation. All the grafts revealed histological characteristics of the proliferative phase, even when the endometrial biopsy was taken during the late secretory phase. Immunohistochemical studies revealed that the proliferation index was high, whatever the menstrual phase of the endometrial biopsy. An extensive vascular network developed at the interface between the graft and the surrounding tissue. Vascular endothelial growth factor (VEGF) positive stained cells were observed in all grafts, the VEGF score being significantly higher in epithelial cells than in stromal cells. In conclusion, human endometrial explants, cultured for 24 h, could be successfully transplanted into nude mice. Immunohistochemical studies proved that human cultured endometrial tissue could not only survive in nude mice but could also become very active and develop characteristics different from the tissue of origin. An active vascular network is a necessary condition for the survival of the graft and may be explained by the high VEGF content. [less ▲]

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See detailExpression of transforming growth factor-alpha, epidermal growth factor, and epidermal growth factor receptor in follicles of human ovarian tissue before and after cryopreservation.
Qu, Jian Ping; NISOLLE, Michelle ULg; Donnez, Jacques

in Fertility and Sterility (2000), 74(1), 113-121

Objective: To study the expression of transforming growth factor-alpha (TGF-α), epidermal growth factor (EGF), and EGF receptor in follicles of human ovarian tissue. Design: A retrospective, controlled ... [more ▼]

Objective: To study the expression of transforming growth factor-alpha (TGF-α), epidermal growth factor (EGF), and EGF receptor in follicles of human ovarian tissue. Design: A retrospective, controlled comparative study. Setting: In vitro fertilization laboratory of a university hospital. Patient(s): Fifteen women with regular menstrual cycles who underwent laparoscopy and the biopsy of ovarian tissue. Intervention(s): Paraffin sections were prepared from ovarian tissues, followed by immunohistochemical staining of TGF-α, EGF, and EGF receptor. Main Outcome Measure(s): Immunostaining for TGF-α, EGF, and EGF receptor in follicles of fresh and frozen ovarian tissues. Result(s): Immunoreactivities for TGF-α and EGF receptor were observed simultaneously in the oocytes of primordial, primary, preantral, and antral follicles. Strong staining for TGF-α and EGF receptor was present in thecal cells. The TGF-α and EGF receptor was also expressed in some granulosa cells of primary to antral follicles. The EGF only stained weakly in the oocytes of primordial and primary follicles and in thecal cells. There was no difference in staining patterns for TGF-α, EGF, and EGF receptor between fresh and frozen ovarian tissues. Conclusion(s): The TGF-α and EGF receptor was expressed in primordial to antral follicles, indicating a role of TGF-α in regulating follicular development through binding to the EGF receptor. Freeze-thawing did not substantially alter immunoreactivites for TGF-α, EGF, and EGF receptor in frozen ovarian tissue. [less ▲]

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See detailHistologic and ultrastructural evaluation of fresh and frozen-thawed human ovarian xenografts in nude mice
NISOLLE, Michelle ULg; Casanas-Roux, Françoise; Qu, Jianping et al

in Fertility and Sterility (2000), 74(1), 122-129

Objective: To compare histologic and ultrastructural characteristics of fresh and frozen-thawed human ovarian cortical tissue grafted into nude mice. Design: Experimental prospective study. Setting: An ... [more ▼]

Objective: To compare histologic and ultrastructural characteristics of fresh and frozen-thawed human ovarian cortical tissue grafted into nude mice. Design: Experimental prospective study. Setting: An academic research environment. Patient(s): Ovarian biopsy specimens were obtained from 13 women undergoing laparoscopy for tubal ligation or infertility. Animal(s): Forty nude mice. Intervention(s): A minilaparotomy was performed to place fresh and frozen-thawed ovarian grafts subcutaneously (sc) or intraperitoneally (ip). Removal of the ovarian grafts was performed at 24 days. Main Outcome Measure(s): [1] the follicular population, [2] fibrosis, [3] vascularization of the grafted tissue, and [4] ultrastructural evaluation. Result(s): A greater fibrosis relative surface area was noted in frozen-thawed transplanted tissue than in fresh transplants. Regardless of this fibrosis, a similar follicular density was observed in fresh and frozen-thawed ovarian tissue 24 days after transplantation. Active angiogenesis was proved by both immunohistochemical study of the vascular endothelial growth factor and morphometric study of the vascular network. Normal ultrastructural characteristics were noted in frozen-thawed ovarian biopsies. Conclusion(s): Angiogenesis allows implantation of the graft even if it has been cryopreserved and thawed similarly to implantation of fresh tissue. The greater fibrosis observed in grafts after cryopreservation and implantation does not seem to affect the primordial and primary ovocyte population and their ultrastructural characteristics, but further studies must be conducted to prove that after cryopreservation and transplantation, ovocytes may achieve full maturation and fertilization. [less ▲]

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See detailLaparascopic management of a unicornuate uterus with two cavitated, non-communicating rudimentary horns
NISOLLE, Michelle ULg; Donnez, Jacques

in Human Reproduction (2000), 15(8), 1873-4

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