References of "Moonen, Gustave"
     in
Bookmark and Share    
See detailNeonatal rat organ of Corti forms supernumerary hair cells in response to growth factor treatment
Lefebvre, P; Malgrange, B; Thiry, Marc ULg et al

Conference (1999)

Detailed reference viewed: 4 (0 ULg)
See detailNeonatal rat organ of Corti forms supernumerary hair cells in response to growth factor treatment
Malgrange, B; Lefebvre, P; Rigo, J-M et al

Poster (1999)

Detailed reference viewed: 7 (0 ULg)
See detailNeonatal rat organ of Corti forms supernumerary hair cells in response to growth factor treatment
Lefèbvre, P; Malgrange, B; Thiry, Marc ULg et al

Conference (1999)

Detailed reference viewed: 5 (0 ULg)
See detailA 295 kDa intermediate filament-associated protein in radial glial and developing muscle cells in vivo and in vitro
Leprince, P; Chanas-Sacré, G; Thiry, Marc ULg et al

Poster (1999)

Detailed reference viewed: 4 (0 ULg)
Full Text
Peer Reviewed
See detailCultured Oligodendrocyte Progenitors Derived from Cerebral Cortex Express a Glycine Receptor Which Is Pharmacologically Distinct from the Neuronal Isoform
Belachew, Shibeshih ULg; Rogister, Bernard ULg; Rigo, Jean-Michel et al

in European Journal of Neuroscience (1998), 10(11), 3556-64

Using the whole-cell patch-clamp technique, we demonstrate glycine-induced currents in oligosphere-derived oligodendrocyte progenitors cultured from newborn rats. Similar inward currents are also ... [more ▼]

Using the whole-cell patch-clamp technique, we demonstrate glycine-induced currents in oligosphere-derived oligodendrocyte progenitors cultured from newborn rats. Similar inward currents are also triggered by beta-alanine and taurine, two established glycine receptor agonists. In our recording conditions, glycine-gated currents in oligodendrocyte progenitors reverse about 0 mV and are reversibly inhibited by the glycine competitive antagonist strychnine, the Cl- channel blocker picrotoxinin and the non-competitive antagonist cyanotriphenylborate. The oligodendrocyte progenitors glycine receptor (GlyR) differs from the corresponding neuronal receptor: [3H]strychnine binding data and the strychnine inhibition curve of glycine-induced currents in oligodendrocyte progenitor cultures suggest the existence of two strychnine binding sites on the oligodendroglial GlyR. Using total RNA isolated from oligodendrocyte progenitors cultures, reverse transcription-polymerase chain reaction analysis of glycine receptor subunit expression shows the presence of alpha2 and beta subunits and immunocytochemical stainings confirm that this GlyR contains an alpha subunit which is not alpha1. The molecular structure of the oligodendroglial GlyR could be either homopentameric alpha2 or heteromeric alpha2beta but in both cases, the sequence of the alpha2 or beta subunits have to be different from the known neuronal sequences in order to explain, respectively, the cyanotriphenylborate (alpha2) and picrotoxinin (beta) sensitivities. This work thus demonstrates that GlyR are expressed by oligodendrocytes obtained not only from spinal cord but also from supraspinal structures. The pharmacological properties and presumably the molecular structure of oligodendroglial GlyR are original. The physiological meaning of the presence of such receptors on developing and mature oligodendrocytes remains unknown. [less ▲]

Detailed reference viewed: 52 (4 ULg)
Peer Reviewed
See detailExpression of Growth Factors and Their Receptors in the Postnatal Rat Cochlea
Malgrange, Brigitte ULg; Rogister, Bernard ULg; Lefebvre, P. P. et al

in Neurochemical Research (1998), 23(8), 1133-8

RT-PCR was used to assay for growth factors and receptors from seven different protein families in cochlea tissues of the juvenile rat. There was a broad representation of the growth factor families in ... [more ▼]

RT-PCR was used to assay for growth factors and receptors from seven different protein families in cochlea tissues of the juvenile rat. There was a broad representation of the growth factor families in all the cochlea tissues examined, though the organ of Corti and stria vascularis expressed a greater variety than the spiral ganglion. This broad expression suggests that a variety of known growth factors play significant roles in the development, maintenance, and repair of the inner ear. The results of this survey serve as a basis for the design of future in vitro experiments that will address the ability of growth factors to protect hair cells from damage and to evoke a repair-regeneration response by injured hair cells. [less ▲]

Detailed reference viewed: 23 (6 ULg)
Full Text
Peer Reviewed
See detailDevelopmental Regulation of Neuroligand-Induced Responses in Cultured Oligodendroglia
Belachew, Shibeshih ULg; Malgrange, Brigitte ULg; Rigo, Jean-Michel et al

in Neuroreport (1998), 9(6), 973-80

Using whole-cell patch-clamp techniques, we show that oligosphere-derived oligodendrocyte progenitor cells (OP) display GABA-, glutamate-, 5-HT-, glycine- and acetylcholine-gated inward currents. When OP ... [more ▼]

Using whole-cell patch-clamp techniques, we show that oligosphere-derived oligodendrocyte progenitor cells (OP) display GABA-, glutamate-, 5-HT-, glycine- and acetylcholine-gated inward currents. When OP differentiate into oligodendrocytes (ODC), the amplitude of peak currents elicited by saturating concentrations of these transmitters decreases except for 5-HT. Intracellular Ca2+ concentration changes induced by microperfusion of glutamate, 5-HT, TRH, met-enkephalin and substance P were monitored using a fluo-3-based calcium imaging system. When OP cells differentiate into ODC, a global decrease of the proportion of responding cells is observed. During type-2 astrocytes commitment, this proportion decreases for 5-HT, TRH- and metenkephalin stimulations whereas it remains constant for substance P and glutamate. These data demonstrate a development regulation of neurotransmitter- and neuropeptide-induced responses within the oligodendroglial lineage. [less ▲]

Detailed reference viewed: 34 (10 ULg)
Full Text
Peer Reviewed
See detailEffects of Macrophage Transplantation in the Injured Adult Rat Spinal Cord: A Combined Immunocytochemical and Biochemical Study
Franzen, Rachelle ULg; Schoenen, Jean ULg; Leprince, Pierre ULg et al

in Journal of Neuroscience Research (1998), 51(3), 316-27

Early and robust invasion by macrophages may be one of the reasons why axonal regeneration is more effective in the PNS than in the CNS. Therefore, we have grafted autologous peritoneal macrophages ... [more ▼]

Early and robust invasion by macrophages may be one of the reasons why axonal regeneration is more effective in the PNS than in the CNS. Therefore, we have grafted autologous peritoneal macrophages labeled with fluorescent latex microspheres into spinal cord compression lesions. At various survival times, we have studied their effect on the expression of neuronal (neurofilaments [NF], calcitonin gene-related peptide [CGRP], 5-hydroxytryptamine [5-HT]) and nonneuronal markers (myelin-associated glycoprotein [MAG], glial fibrillary acidic protein [GFAP], laminin) by using semiquantitative Western blot and immunohistochemical techniques. After 1 month, we observed a significant decrease of the expression of MAG as well as an important invasion of the lesion site by neurites, chiefly peptidergic axons of presumed dorsal root origin, in macrophage-grafted animals compared with controls. In addition, angiogenesis and Schwann cell infiltration were more pronounced after macrophage grafts, providing an increase in laminin, a favorable substrate for axonal regrowth. By using reverse transcription-polymerase chain reaction (RT-PCR), mRNAs for tumor necrosis factor-alpha (TNF-alpha) were detected in the transplanted cells, whereas results were negative for nerve growth factor (NGF), neurotrophin-3 (NT-3), brain-derived neurotrophic factor (BDNF), or acidic fibroblast growth factor (aFGF) and basic fibroblast growth factor (bFGF). Thus, macrophage grafts may represent an interesting strategy to promote axonal regeneration in the CNS. Our study suggests that they may exert their beneficial effects by degrading myelin products, which inhibit axonal regrowth, and by promoting a permissive extracellular matrix containing notably laminin. No evidence for a direct synthesis of neurotrophic factors by the transplanted macrophages was found in this study, but resident glial cells could secrete such factors as a result of stimulation by macrophage-released cytokines. [less ▲]

Detailed reference viewed: 18 (1 ULg)
Full Text
Peer Reviewed
See detailIn vitro ototoxicity of aminoglycosides and platin derivatives. A semi-automatic assay for sensory hair cell damage in explanted rat organ of corti.
Malgrange, B.; LEFEBVRE, Philippe ULg; van de Water, T.R. et al

in Toxicology in Vitro (1998), 12 (6)

The ototoxic damage that drugs such as neomycin, kanamycin, colistin, cisplatin, transplatin and carboplatin cause on outer and inner hair cells in postnatal day 3 rat cochlear explants was investigated ... [more ▼]

The ototoxic damage that drugs such as neomycin, kanamycin, colistin, cisplatin, transplatin and carboplatin cause on outer and inner hair cells in postnatal day 3 rat cochlear explants was investigated. Phalloidin-fluorescein conjugate-stained stereocilia bundles of sensory hair cells were quantified by video image analysis as a measurement of ototoxic effect. The video image quantification system established dose-response curves for ototoxic drugs (e.g. calculation of an IC50) and allowed comparisons between several ototoxins from the same family. This methodology provided the means to assess the efficacy of otoprotectant agents in preventing ototoxicity. Poly-l-aspartate (10-5M) and poly-l-glutamate (10-5M) protected auditory hair cells from neomycin (10-3M) toxicity while reduced glutathione (10-3M) provided protection against cisplatin (10-4M)-induced hair cell damage. [less ▲]

Detailed reference viewed: 25 (1 ULg)
See detailThe radial glial cell antigen recognized by the RC2 antibody is an intermediate-filament associated protein
Chanas-Sacré, G; Leprince, P; Lewin, M et al

Poster (1998)

Detailed reference viewed: 2 (0 ULg)
Peer Reviewed
See detailSpontaneous longitudinally orientated axonal regeneration is associated with the Schwann cell framework within the lesion site following spinal cord compression injury of the rat.
Brook, G. A.; Plate, D.; Franzen, Rachelle ULg et al

in Journal of Neuroscience Research (1998), 53(1), 51-65

Spontaneous cellular reorganisation at the lesion site has been investigated following massive spinal cord compression injury in adult rats. By 2 days post operation (p.o.), haemorrhagic necrosis ... [more ▼]

Spontaneous cellular reorganisation at the lesion site has been investigated following massive spinal cord compression injury in adult rats. By 2 days post operation (p.o.), haemorrhagic necrosis, widespread axonal degeneration, and infiltration by polymorphnuclear granulocytes and OX42-positive macrophages were observed in the lesion site. By 7 days p.o., low affinity nerve growth factor receptor-positive Schwann cells, from activated spinal roots, were identified as they migrated far into the lesion. Between 7 and 14 days p.o., the overlapping processes of Schwann cells within the macrophage-filled lesion formed a glial framework which was associated with extensive longitudinally orientated ingrowth by many neurofilament-positive axons. Relatively few of these axons were calcitonin gene-related peptide (CGRP)-, substance P (SP)-, or serotonin (5HT)-positive; however, many were glycinergic or gamma aminobutyric acid (GABA)ergic. At 21 and 28 days p.o. (the longest survival times studied), a reduced but still substantial amount of orientated Schwann cells and axons could be detected at distances of up to 5 mm within the lesion. Glial fibrillary acidic protein (GFAP) immunoreactivity demonstrated the slow formation of astrocytic scarring which only became apparent at the lesion interface between 21 and 28 days p.o. The current data suggest the possibility of developing future therapeutic strategies designed to maintain or even enhance these spontaneous and orientated regenerative events. [less ▲]

Detailed reference viewed: 19 (0 ULg)
Peer Reviewed
See detailSpontaneous axonal regeneration into the lesioned site following closed contusion injury to the adult rat spinal cord.
Brook, G.; Plate, D.; Schmitt, A. B. et al

Conference (1997, October)

Detailed reference viewed: 4 (0 ULg)
Peer Reviewed
See detailSpinal injury and spinal cord injury
Martin, Didier ULg; Moonen, Gustave ULg

Conference (1997, September 12)

Detailed reference viewed: 2 (0 ULg)
Full Text
Peer Reviewed
See detailDiazepam-Insensitive Gabaa Receptors on Postnatal Spiral Ganglion Neurones in Culture
Malgrange, Brigitte ULg; Rigo, Jean-Michel; Lefebvre, Philippe ULg et al

in Neuroreport (1997), 8(3), 591-6

Using dissociated spiral ganglion cell cultures obtained from 3-day-old rat cochlea, we investigated the response of auditory neurones to gamma-aminobutyric acid (GABA) using patch-clamp techniques. In ... [more ▼]

Using dissociated spiral ganglion cell cultures obtained from 3-day-old rat cochlea, we investigated the response of auditory neurones to gamma-aminobutyric acid (GABA) using patch-clamp techniques. In our recording conditions, GABA elicited inward currents in > 95% of the neurones which reversed around 0 mV. Similar inward currents were measured using isoguvacin, a specific agonist of GABAA receptors. GABA-gated currents were reversibly inhibited by the channel blocker picrotoxin and the GABA competitive antagonist bicuculline. These functional GABAA receptors are characterized by an insensitivity to benzodiazepines and a relatively high sensitivity to beta-carbolines and barbiturates. These results show that the GABAA receptor pharmacological properties of spiral ganglion neurones are close to those of cerebellar granule cells. [less ▲]

Detailed reference viewed: 62 (9 ULg)
Peer Reviewed
See detailEffect of neuropeptides on cultured postnatal auditory neurons.
Malgrange, Brigitte ULg; Lefebvre, P.P.; Rigo, J.M. et al

Conference (1997)

Detailed reference viewed: 19 (7 ULg)