Démarche diagnostique face aux dermatoses prurigineuses chez le chien

Conference given outside the academic context (2005)

Nouvelles recherches sur la caractérisation des facteurs de virulence de Microsporum canis.

in Bulletin et Mémoires de l'Académie Royale de Médecine de Belgique (2005), 160(5-6), 270-275

Microsporum canis is a worldwide distributed zoophilic and zoonotic dermatophyte which is responsible for most cases of feline ringworm. Its prevalence is on the rise in developed countries. Since 1995 ... [more ▼]

Microsporum canis is a worldwide distributed zoophilic and zoonotic dermatophyte which is responsible for most cases of feline ringworm. Its prevalence is on the rise in developed countries. Since 1995, the author and his Laboratory have developed a research programme to better understand the pathogenesis of M. canis dermatophytosis. Both the potential fungal virulence factors and the host immune response were investigated. A 31.5 kDa keratinolytic subtilase (SUB) and a 43.5 kDa keratinolytic metalloprotease (MEP) were purified from a crude exoantigen consisted in a mixture of inducible proteins secreted by the fungus in a medium enriched with keratin. Additionally, two gene families encoding respectively 3 SUBs and 3 MEPs, including the 2 keratinases previously purified (called SUB3 and MEP3), were characterized. The genes encoding several MEPs and SUBs were shown to be transcribed in vivo during infection by nested RT-PCR. Finally, we demonstrated that a specific cellular immune response towards the crude exoantigen, MEP3 and SUB3 was induced in experimentally infected guinea pigs. This indicates that the potential immunoprotective properties of these antigens are interesting to evaluate in the frame of the development of an efficient vaccine against feline ringworm. [less ▲]

A randomized, double-blinded, placebo-controlled study of the efficacy of selamectin in the control of Chirodiscoides caviae infestation in guinea pigs

Poster (2005)

Mange in cats and dogs

in Merck Veterinary Manual (MVM) (2005)

Anti-fungal therapy Workshop: efficacy of commercial vaccines (Dermatophytosis)

in Advances in Veterinary Dermatology (2005)

Le microscope au quotidien pour le diagnostic des dermatoses parasitaires et infectieuses chez l'animal de compagnie

Conference given outside the academic context (2005)

A case of sylvatic dermatophytosis due to Trichophyton mentagrophytes in a cat

Poster (2005)

Biological control of Psoroptes ovis using entomopathogenic fungi

Poster (2005)

Secreted subtilisin gene family in Trichophyton rubrum

in Gene (2004), 339

Secreted proteases constitute potential virulence factors of dermatophytes. A total of seven genes encoding putative serine proteases of the subtilisin family (SUB) were isolated in Trichophyton rubrum ... [more ▼]

Secreted proteases constitute potential virulence factors of dermatophytes. A total of seven genes encoding putative serine proteases of the subtilisin family (SUB) were isolated in Trichophyton rubrum. Based on sequence data and intron-exon structure, a phylogenetic analysis of subtilisins from T rubrum and other fungi revealed a presumed ancestral lineage comprising T rubrum SUB2 and Aspergillus SUBs. All other SUBs (SUB1, SUB3-7) are dermatophyte-specific and have apparently emerged more recently, through successive gene duplication events. We showed that two subtilisins, Sub3 and Sub4, were detected in culture supernatants of T rubrum grown in a medium containing soy protein as a sole nitrogen source. Both recombinant enzymes produced in Pichia pastoris are highly active on keratin azure suggesting that these proteases play an important role in invasion of keratinised tissues by the fungus. The set of deduced amino acid sequences of T rubrum SUB ORFs allowed the identification of orthologous Subs secreted by other dermatophyte species using proteolysis and mass spectrometry. (C) 2004 Elsevier B.V. All rights reserved. [less ▲]

Recent data on a current zoonosis: alveolar echinococcus due to Echinococcus multilocularis

in Annales de Médecine Vétérinaire (2004), 148(4), 153-167

E. multilocularis is a small tapeworm which uses different species of foxes and rodents as final and intermediate hosts respectively. In humans, infection with the metacestode stage can result in a ... [more ▼]

E. multilocularis is a small tapeworm which uses different species of foxes and rodents as final and intermediate hosts respectively. In humans, infection with the metacestode stage can result in a potentially lethal liver condition named alveolar echinococcosis (AE). Recent studies in central Europe have show that E. multilocularis has a wider geographic range than previously anticipated. By the end of the 1980s, endemic areas of E. multilocularis were know to exist in only four countries, but now the parasite is present in at least 14 European countries. This paper provide recent data on the biology of E. multilocularis, the epidemiology of AE and the different tools which are available to treat or prevent this disease which seems in apparent extension. [less ▲]