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See detailPurification and Characterization of a 315 Kda Keratinolytic Subtilisin-Like Serine Protease from Microsporum Canis and Evidence of Its Secretion in Naturally Infected Cats
Mignon, Bernard ULg; Swinnen, M.; Bouchara, J. P. et al

in Medical Mycology (1998), 36(6), 395-404

A keratinolytic protease, secreted as the major component by a feline clinical isolate of Microsporum canis cultivated in a minimal medium containing cat keratin, was purified by affinity chromatography ... [more ▼]

A keratinolytic protease, secreted as the major component by a feline clinical isolate of Microsporum canis cultivated in a minimal medium containing cat keratin, was purified by affinity chromatography on bacitracin agarose and gel filtration. The apparent molecular mass of the enzyme was 31.5 kDa and the pI was 11.8. The enzyme was not glycosylated and its first 15 N-terminal amino acids showed numerous similarities with other fungal subtilisins. The optimum pH was around 9 while inactivation of the enzyme was reversible at pH 4, but not at pH 11. The enzyme was stable at 37 degrees C with an apparent optimum temperature around 55 degrees C. PMSF, soybean trypsin inhibitor (SBTI) and chymostatin strongly inhibited the proteinase. The highest affinity (Km of 0.37 mM) and physiological efficiency (k(cat)/Km) were obtained for the synthetic substrate N-Suc-Ala-Ala-Pro-Phe-p-nitroanilide. These results indicate that the keratinase belongs to the subtilisin-like serine protease family. Purified rabbit immunoglobulins G prepared against the keratinase and used in an immunohistochemical test allowed the detection of the keratinase produced by the fungus invading hair structures in naturally infected cats. The in vitro keratinolytic activity of the enzyme and its production in vivo suggest that it may contribute to pathogenicity. [less ▲]

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See detailResponse to 'Comments on Microsporum Canis'
Mignon, Bernard ULg

in Medical Mycology (1998), 36(4), 248

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See detailField Efficacy of Injectable Doramectin against Chorioptes Bovis in Naturally Infected Cattle
Losson, Bertrand ULg; Mignon, Bernard ULg; Bossaert, K. et al

in Veterinary Record : Journal of the British Veterinary Association (1998), 142(1), 18-19

A single subcutaneous injection of doramectin at a dose rate of 200 micrograms/kg bodyweight was effective in controlling an infection of Chorioptes bovis mites in naturally infected cattle. From 14 days ... [more ▼]

A single subcutaneous injection of doramectin at a dose rate of 200 micrograms/kg bodyweight was effective in controlling an infection of Chorioptes bovis mites in naturally infected cattle. From 14 days after treatment, the geometric mean number of live mites was significantly lower (P < 0.001) in the doramectin-treated cattle than in the control group at each sampling until day 35. The percentage efficacy (treated versus controls) of doramectin against C bovis at day 35 was 99.9 per cent and the percentage reduction (day 35 versus day 0) in the treated animals was 99.3 per cent. At day 35, all seven controls were still positive for C bovis whereas five of the eight doramectin-treated animals were free of live mites. [less ▲]

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See detailDonnées récentes sur les trichophyties bovines
Mignon, Bernard ULg

Scientific conference (1998)

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See detailActualités sur les helminthoses des carnivores domestiques
Mignon, Bernard ULg

Conference given outside the academic context (1998)

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See detailActualités sur les helminthoses digestives des carnivores domestiques
Mignon, Bernard ULg

Conference given outside the academic context (1998)

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See detailThe in vitro and in vivo production of a 31.5-kD keratinolytic subtilase from Microsporum canis and the clinical status in naturally infected cats.
Mignon, Bernard ULg; Nikkels, Arjen ULg; Pierard, Gérald ULg et al

in Dermatology : International Journal for Clinical & Investigative Dermatology (1998), 196(4), 438-441

BACKGROUND: Microsporum-canis-infected cats, especially the asymptomatic infected ones, are mainly responsible for the zoonotic disease. The important variability of the clinical signs in cats is poorly ... [more ▼]

BACKGROUND: Microsporum-canis-infected cats, especially the asymptomatic infected ones, are mainly responsible for the zoonotic disease. The important variability of the clinical signs in cats is poorly understood. Recently, a 31.5-kD keratinolytic subtilase was found to be a putative virulence factor. OBJECTIVE: To investigate the possible relationship between the clinical status of dermatophytic cats and the production of the keratinase. METHODS: Seven M. canis strains isolated either from clinically affected, asymptomatic infected or mechanical carrier cats were tested for the in vitro production of the enzyme. The immunohistochemical detection of the enzyme was also assessed in skin biopsies of 4 symptomatic and 7 asymptomatic naturally infected cats. RESULTS: All the strains produced in vitro a 31.5-kD keratinolytic subtilase. The enzyme was present in all but 1 of the infected cats. CONCLUSION: The production of the keratinase is not a factor directly responsible for the clinical picture seen in M.-canis-infected cats. [less ▲]

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See detailLes tiques et leur rôle vectoriel
Losson, Bertrand ULg; Mignon, Bernard ULg

in Pharmasphère (1998), 25

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See detailResearch on epizootology and prevention of hypodermiasis in cattle in Transylvania, Romania
Cozma, V.; Suteu, I.; Losson, Bertrand ULg et al

in Buletinul Universitatii de Stiinte Agricole si Medicina Veterinara Cluj-Napoca (1998), 52

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See detailPrevalence and characterization of Microsporum canis carriage in cats.
Mignon, Bernard ULg; Losson, Bertrand ULg

in Journal of Medical and Veterinary Mycology : Bi-Monthly Publication of the International Society for Human and Animal Mycology (1997), 35(4), 249-256

In order to determine the prevalence and to characterize the carriage of Microsporum canis in cats, different mycological examinations (including a culture obtained by hair brushing and Wood's light ... [more ▼]

In order to determine the prevalence and to characterize the carriage of Microsporum canis in cats, different mycological examinations (including a culture obtained by hair brushing and Wood's light examination) were performed on 632 animals of different origins. Group 1 comprised 467 healthy pet cats belonging to veterinary students. In this group, prevalence of carriage was 2.1%: eight cats were asymptomatic transient carriers and one cat was an asymptomatic infected animal presenting discrete Wood's-positive lesions disseminated on the whole body that were visible after sedation and clipping. The carriage prevalence was higher (15.7%) in group 2 comprising 134 European cats destroyed in a pound and kept together. In two additional groups of cats, it was shown that an infected cat was responsible for the dissemination of fungal material into its environment including the other in-contact animals. When the active source of fungus was removed, the dissemination stopped, resulting in a decrease in the amount of infective material recovered from both the animal carriers and the environmental surfaces. This was also observed in two experimental groups of guinea pigs. No association between feline immunodeficiency virus infection and the M. canis carriage was observed in a retrospective case-control study performed on group 2. None of these cats was feline leukaemia virus positive. [less ▲]

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